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A molecular marker of leaf rust resistance gene Lr37 in wheat

Published online by Cambridge University Press:  12 February 2007

Zhang Li-Rong ,
Xu Da-Qing ,
Yang Wen-Xiang  and
Liu Da-Qun
Zhang Li-Rong
Affiliation:
Biological Centre of Plant Pathogens and Plant Pest, Department of Plant Pathology, College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China
Xu Da-Qing
Affiliation:
Biological Centre of Plant Pathogens and Plant Pest, Department of Plant Pathology, College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China
Yang Wen-Xiang
Affiliation:
Biological Centre of Plant Pathogens and Plant Pest, Department of Plant Pathology, College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China
Liu Da-Qun*
Affiliation:
Biological Centre of Plant Pathogens and Plant Pest, Department of Plant Pathology, College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China
*
*Corresponding author: E-mail: ldq@mail.hebau.edu.cn
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Abstract

Inter-simple sequence repeat (ISSR) analysis was carried out in Thatcher, 20 near-isogenic lines (NILs) containing respectively different genes conferring resistance against wheat leaf rust (Puccinia recondite f.sp. tritici), three materials carrying Lr37 and three materials without Lr37. All of the 100 ISSR primers showed clear amplification products. Two of them amplified the polymorphic DNA bands in the NILs, Thatcher and Lr37/6*Thatcher. The polymorphic bands were named UBC812-1200 and UBC848-700, respectively. The three materials with and without Lr37 were detected in tests using the two primers UBC812 and UBC848. Results also showed that only band UBC812-1200 was amplified in all resistant and absent in all susceptible materials. This suggests that UBC812-1200 marker is linked to the resistance gene Lr37. The genetic linkage of the polymorphic marker with Lr37 was tested using a segregating F2 population (128 plants) derived from a cross between the leaf rust-resistant Lr37/6*Thatcher and the susceptible cultivar Thatcher. The ISSR marker UBC812-1200 showed co-segregation to the Lr37 resistance gene. It could be used in molecular marker-assisted selection in a wheat breeding programme for leaf rust resistance.

Keywords

wheat leaf rustresistance gene Lr37inter-simple sequence repeat marker
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 1 , Issue 3 , December 2004 , pp. 139 - 142
Copyright
Copyright © China Agricultural University and Cambridge University Press 2004

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References

Chen, WQ and Wang, JX (1997) Genes for leaf and stem rust resistance in 76 wheat genetic resources. Acta Agronomica Sinica 23, 655–663 in Chinese with English abstract.Google Scholar
Gill, KS, Lubbers, EL and Raupp, WJ et al. (1991) A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome 34, 362–374.CrossRefGoogle Scholar
Kloppers, FJ and Pretorius, ZA (1997) Effects of combinations among Lr 13, Lr 34, and Lr 37 on components of adult plant resistance in wheat to leaf rust. Plant Pathology 46, 737–750.CrossRefGoogle Scholar
Kolmer, JA (1996) Genetics of resistance to wheat leaf rust. Annual Review of Phytopathology 34, 435–455.CrossRefGoogle ScholarPubMed
Liu, ZY, Wang, XL and Ni, ZF et al. (2000) Molecular marker assisted selection (MAS) of leaf rust ( Puccinia recondita f.sp. tritici ) resistance genes, Lr 9 and Lr 24, in wheat. Journal of Agricultural Biotechnology 8, 13–16 in Chinese with English abstract.Google Scholar
Seyfarth, R, Feuillet, C and Schachermayr, G et al. (1999) Development of a molecular marker for the adult plant leaf rust resistance gene Lr35 in wheat. Theoretical and Applied Genetics 99, 554–560CrossRefGoogle ScholarPubMed