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Resistance Mechanisms to an Acetolactate Synthase (ALS) Inhibitor in Water Starwort (Myosoton aquaticum) Populations from China

Published online by Cambridge University Press:  20 January 2017

Weitang Liu ,
Cuixia Wu ,
Wenlei Guo ,
Long Du ,
Guohui Yuan  and
Jinxin Wang
Weitang Liu
Affiliation:
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai'an 271018, PR China
Cuixia Wu
Affiliation:
Taian Academy of Agricultural Sciences, Tai'an 271000, Shandong, PR China, PR China
Wenlei Guo
Affiliation:
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai'an 271018, PR China
Long Du
Affiliation:
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai'an 271018, PR China
Guohui Yuan
Affiliation:
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai'an 271018, PR China
Jinxin Wang*
Affiliation:
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Shandong Tai'an 271018, PR China
*
Corresponding author's E-mail: wangjx@sdau.edu.cn
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Abstract

Overreliance on tribenuron has resulted in resistance evolution in water starwort. This study investigates the resistance mechanisms to tribenuron in water starwort populations from China. The cytochrome P450 monooxygenase (P450) inhibitor malathion increased tribenuron sensitivity in all populations. The decrease in the amount of herbicide dose that causes 50% growth reduction (GR50) for the sensitive (S) population JS24 and the resistant (R) populations JS16 and JS17 were 2.3-, 2.5-, and 4.1-fold, respectively. However, the GR50 values for the R populations were still much higher than those of the S population. This observation indicates that P450-mediated enhanced metabolism is one mechanism for resistance in water starwort. The glutathione-S-transferase (GST) activity could be induced by tribenuron for all tested populations. In particular, the GST activity of JS16 is inherently greater and is more rapidly induced than that of JS17 or JS24. Resistance attributed to mutant acetolactate synthase (ALS) alleles was identified by sequence analysis for each population. Pro197Ser substitution was detected in JS16 and JS17. Molecular markers were also developed to rapidly identify resistance as well as individuals carrying the specific Pro197Ser mutation in water starwort populations. The resistance patterns experiment revealed that the R populations exhibited different levels of resistance to pyrithiobac sodium salt, florasulam, pyroxsulam, and flucarbazone-Na; however, R populations were sensitive to imazethapyr, fluroxypyr-meptyl, 2,4-D butylate, isoproturon, and diflufenican. This study establishes that either one or at least two resistance mechanisms are involved in herbicide resistance in water starwort. Moreover, these mechanisms might contribute to the different levels of resistance to tribenuron among water starwort populations.

Keywords

2,4-D butylatediflufenicanflorasulamflucarbazone-Nafluroxypyr-meptylimazethapyrisoproturonpyrithiobac sodium saltpyroxsulamtribenuronwater starwort, Myosoton aquaticum (L.) Moench.ALSdCAPSherbicide resistanceresistance mechanismsresistance pattern
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 63 , Issue 4 , December 2015 , pp. 770 - 780
Copyright
Copyright © Weed Science Society of America 

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