Hostname: page-component-8448b6f56d-cfpbc Total loading time: 0 Render date: 2024-04-16T13:13:07.036Z Has data issue: false hasContentIssue false
  • English
  • Français

A System for Studying Carbon Allocation in Plants Using 11C-Labeled Carbon Dioxide

Published online by Cambridge University Press:  18 July 2016

Youhanna Fares ,
J D Goeschl ,
C E Magnuson ,
C E Nelson ,
B R Strain ,
C H Jaeger  and
E G Bilpuch
Youhanna Fares
Affiliation:
Biosystems Research Division, Industrial Engineering Department, Texas A&M University, College Station, Texas 77843
J D Goeschl
Affiliation:
Biosystems Research Division, Industrial Engineering Department, Texas A&M University, College Station, Texas 77843
C E Magnuson
Affiliation:
Botany Department and Phytotron, Duke University, Durham, North Carolina 27706
C E Nelson
Affiliation:
Department of Radiology and Physics, Duke University
B R Strain
Affiliation:
Botany Department and Phytotron, Duke University, Durham, North Carolina 27706
C H Jaeger
Affiliation:
Botany Department and Phytotron, Duke University, Durham, North Carolina 27706
E G Bilpuch
Affiliation:
Physics Department, Duke University
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The effects of environmental factors such as water stress, elevated CO2, or temperature on carbon assimilation and allocation in plants have been studied extensively (Gifford and Evans, 1981; Loomis, Rabbinge, and Ng, 1979; Neales and Incoll, 1968). However, the interactions of these processes are not well understood and cannot be predicted with any degree of confidence. Continuous and simultaneous measurements of photosynthesis, transport, and sink activity have never been made during the short- and long-term responses of live, intact plants to step changes in environmental factors. Thus, direct environmental effects and adaptive responses of plants are generally not distinguished. This results in part from limitation in experimental techniques and protocol used in past studies and the lack of experimental validation of hypotheses and models (eg, Goeschl et al, 1976; Magnuson et al, 1979; Smith et al, 1970) dealing with these problems. This paper describes in detail the components of an integrated technique for studying carbon assimilation, transportation and allocation in intact live plants under any set of environmental conditions, using continuously produced 11CO2.

Type
III. 14C Applications
Information
Radiocarbon , Volume 25 , Issue 2: 11th International Radiocarbon Conference , 1983 , pp. 429 - 439
Copyright
Copyright © The American Journal of Science 

References

Cirilov, SD, Newton, JD, and Schapira, JP, 1966, Total cross sections for the reaction 12C (3He, 4He) 11C and 12C (3He, n) 14O: Nuclear Physics, v 77, p 472–476.CrossRefGoogle Scholar
Downs, RJ, Hellmers, H, and Kramer, PJ, 1972, Engineering problems in the design and operation of phytotrons: Am Soc of Heating Refrig and Air Conditioning Engineers Jour, June 1972, p 47–55.Google Scholar
Fares, Y, DeMichele, DW, Goeschl, JD, and Baltuskonis, DA, 1978, Continuously produced, high specific activity 11C for studies of photosynthesis, transport and metabolism: Internatl Jour Appl Radiation Isotopes, v 29, p 431–441.CrossRefGoogle Scholar
Gifford, RM, and Evans, LT, 1981, Photosynthesis, carbon partitioning, and yield: Ann Rev Plant Physiol, v 32, p 485–509.Google Scholar
Goeschl, JD, Magnuson, CE, DeMichele, DW and Sharpe, PJH, 1976, Concentration dependent unloading as a necessary assumption for a closed-form mathematical model of osmotically driven pressure-flow in phloem: Plant Physiol, v 58, p 556–562.CrossRefGoogle ScholarPubMed
Kramer, PJ, Hellmers, H, and Downs, RJ, 1970, SEPEL: New phytotrons for environmental research: Bioscience, v 20, no. 22, p 1201–1208.Google Scholar
Loomis, RS, Rabbinge, R, and Ng, E, 1979, Explanatory models in crop physiology: Ann Rev Plant Physiol, v 30, p 339–367.CrossRefGoogle Scholar
Magnuson, CE, Goeschl, JD, Sharpe, PJH and DeMichele, DW, 1979, Consequences of insufficient equations in model of the Munch hypothesis of phloem transport: Plant Cell and Environm, v 2, p 181–188.Google Scholar
Magnuson, CE, Fares, Y, Goeschl, JD, Nelson, CE, Strain, DR, Jaeger, CH and Bilpuch, EG, 1982, An integrated tracer kinetics system for studying carbon uptake and allocation in plants using continuously produced 11CO2 : Rad and Environm Biophys, in press.Google Scholar
Neales, TF, and Incoll, LD, 1968, The control of leaf photosynthesis rate by level of assimilate concentration in the leaf: A review of the hypothesis: Bot Rev, v 34, p 107–125.Google Scholar
Smith, KC, Magnuson, CE, Goeschl, JD and DeMichele, DW, 1980, A time-dependent mathematical expression of the Munch hypothesis of phloem transport: Jour Theor Biol, v 86, p 493–505.Google Scholar