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Spatial Marking and Quantitative Counting Cell Membrane αIIbβ3 Integrin in Single Cells via Peptide-Au Cluster Probes

Published online by Cambridge University Press:  25 May 2015

Lina Zhao  and
Xueyun Gao
Lina Zhao
Affiliation:
Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China
Xueyun Gao*
Affiliation:
Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China
*
*email: gaoxy@ihep.ac.cn
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Abstract

We propose a theoretical designed peptide-Au cluster probe and realize spatially marking and quantitatively counting αIIbβ3 integrin via this novel nanoprobe. On single cell level, we find the number of αIIbβ3 integrin ranges from 5.75 to 9.11×10-17 mol for the heteroexpression of individual cells. Because the variation of proteins (including integrin and other biomarkers) on single cell has a tight correlation with many serious human diseases, our quantifying protein method on a single cell level is helpful for estimating the disease progression for diagnosis and detecting the drug response for therapy.

Keywords

biomedicalnanostructureAu
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1802: Symposium PP – Gold-Based Materials and Applications , 2015 , pp. 25 - 31
Copyright
Copyright © Materials Research Society 2015 

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References

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