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Dietary phenolics other than anthocyanins inhibit PTP1B; an in vitro and in silico validation

Published online by Cambridge University Press:  07 March 2019

S.K. Barik
Affiliation:
The Rowett Institute, University of Aberdeen, Aberdeen, AB25 2ZD, United Kingdom
W.R. Russell
Affiliation:
The Rowett Institute, University of Aberdeen, Aberdeen, AB25 2ZD, United Kingdom
B. Dehury
Affiliation:
RMRC, Indian Council of Medical Research, Bhubaneswar, 751023, Odisha, India
M. Cruickshank
Affiliation:
The Rowett Institute, University of Aberdeen, Aberdeen, AB25 2ZD, United Kingdom
K.M. Moar
Affiliation:
The Rowett Institute, University of Aberdeen, Aberdeen, AB25 2ZD, United Kingdom
D. Thapa
Affiliation:
The Rowett Institute, University of Aberdeen, Aberdeen, AB25 2ZD, United Kingdom
N. Hoggard
Affiliation:
The Rowett Institute, University of Aberdeen, Aberdeen, AB25 2ZD, United Kingdom
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Abstract

Type
Abstract
Copyright
Copyright © The Authors 2019 

Previous reports suggest that berries rich in dietary anthocyanins are able to inhibit protein tyrosine phosphatase (PTP1B), an important target in the prevention of type 2 diabetes(Reference Xiao, Guo and Sun1, Reference Zhao, Duc Hung and Le2). Black and green currants (Ribes sp.) were selected due to their significant difference in anthocyanin content(Reference Mattila, Hellstrom and Karhu3), which allowed us to assess if the metaboloites produced following in vitro gastrointestinal digestion (IVGD) are responsible for PTP1B inhibition.

Freeze-dried black and green currant extracts underwent IVDG(Reference Minekus, Alminger and Alvito4). The predominant metabolites produced were identified by LC-MS/MS and these and the digested extracts were screened for their ability to inhibit human PTP1B in vitro(Reference Uddin, Sharma and Yang5). For the most effective inhibitor, Lineweaver-Burk and Dixon plots were employed at mean reciprocal of initial velocity for n = 3 replicates at several substrate concentrations. Further, targeted molecular docking was used to study the ligand-protein interaction of the same compound using AutoDock Vina V4.2(Reference Dehury, Behera and Mahapatra6).

IVGD black and green currant extracts at a physiological relevant concentration inhibited PTP1B by 60.2 % (P < 0.001), IC50 11.42 ± 0.16 μg/mL and 45.7  % (P < 0.001), IC50 19.98 ± 0.21 μg/mL respectively. This suggests that anthocyanins may not be the key inhibitors of PTP1B. Phytochemical analysis identified several predominant phenolics, which were further studied to identify their individual ability to inhibit PTP1B. Gallic acid at a physiological relevant concentration inhibited PTP1B by 52.9 % (P < 0.001, IC50 56.37 ± 1.03 μg/mL), followed by 4-hydroxybenzaldehyde (38.9 %, P > 0.001), ferulic acid (37.7 %, P < 0.001) resveratrol (37.3 %, P < 0.001) and chlorogenic acid (36.1 %, P < 0.001). Sodium orthovanadate (positive inhibitor) at the same concentration inhibited PTP1B by 56.5 % (P = 0.0034, IC50 4.65 ± 0.80 μg/mL). Co-incubation of the phenolics did not increase PTP1B inhibition when compared to their individual compounds suggesting a non-synergistic effect. Phytochemical analysis identified that gallic acid was the most abundant phenolic (14.9 %) compound measured in green currants. Moreover, enzyme kinetics presented that gallic acid inhibits PTP1B non-competitively (Ki = 33.3 ± 1.67 μg/mL) while the in silico study identified the binding affinity of gallic acid to PTP1B's active site to be -6.37 kcal/mole. In conclusion, we have shown that several phenolic compounds from the currants are able to inhibit human PTP1B in vitro with gallic acid having the highest activity.

The University of Aberdeen and Nutricia Research Foundation funded the doctoral studentship to SKB. The research was supported by the Rural Affairs Food and the Environment Strategic Research (RESAS). Authors are thankful to the James Hutton Institute, Dundee for providing the currants and Dr Graham Horgan (BioSS) for his assistance during statistical analysis of the study.

References

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