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SELENIUM FORTIFICATION AND PRO/ANTI OXIDANT RESPONSES IN ALLIUM CEPA (ONION) CULTIVATED IN Se SUPPLEMENTED SOILS

Published online by Cambridge University Press:  21 July 2010

N. TEJO PRAKASH*
Affiliation:
Department of Biotechnology and Environmental Sciences, Thapar University, Patiala – 147004, India
NEETU SHARMA
Affiliation:
Department of Biotechnology and Environmental Sciences, Thapar University, Patiala – 147004, India
RANJANA PRAKASH
Affiliation:
School of Chemistry and Biochemistry, Thapar University, Patiala – 147004, India
T. N. NATHANIEL
Affiliation:
Radiochemistry Division, Bhabha Atomic Research Centre, Mumbai – 400085, India
R. ACHARYA
Affiliation:
Radiochemistry Division, Bhabha Atomic Research Centre, Mumbai – 400085, India
A. V. R. REDDY
Affiliation:
Analytical Chemistry Division, Bhabha Atomic Research Centre, Mumbai – 400085, India
*
Corresponding author. ntejoprakash@thapar.edu

Summary

Native plantlets of Allium cepa (onion) were cultivated in selenium (Se)-supplemented soils to observe dose- and time-dependent uptake and associated pro/anti-oxidant activity. Allium plantlets were grown in soils supplemented with Se at 2.5, 5.0 and 7.5 mg kg−1 as selenite (Na2SeO3) and selenate (Na2SeO4) for 40–120 days. The effect of different concentrations of Se oxyanions on the growth and accumulation profile in plants in relation to glutathione peroxidase activity and thiobarbituric acid reactive substances was studied. Total Se concentrations in plantlets were determined by instrumental neutron activation analysis. The accumulation of Se in plant was observed to be dose dependent in the case of selenite, but, no definite correlation between accumulation levels and exposed concentration was observed. The changes in pro/anti-oxidant properties were observed to be dependent on the accumulation of Se in plant.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2010

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