Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-cb9f654ff-hn9fh Total loading time: 0 Render date: 2025-08-08T02:44:35.684Z Has data issue: false hasContentIssue false

Chapter 11 - Zona Binding: Competitive Sperm-Binding Assay

Published online by Cambridge University Press:  05 April 2021

By
De Yi Liu ,
Ying Zhong  and
Zi-Na Wen
Edited by
Ashok Agarwal ,
Ralf Henkel  and
Ahmad Majzoub
Ashok Agarwal
Affiliation:
The Cleveland Clinic Foundation, Cleveland, OH
Ralf Henkel
Affiliation:
University of the Western Cape, South Africa
Ahmad Majzoub
Affiliation:
Hamad Medical Corporation, Doha
Get access

Summary

Today, about 10–15 percent of couples at reproductive age worldwide are infertile and they are unable to conceive naturally without medical assistance. Infertility can be caused by male-only factors, female-only factors or a combination of both. However, the cause of infertility is currently unidentifiable in about 20–30 percent of patients, who are classified as “unexplained infertility”. Currently, there is a lack of effective medical treatment for most infertile couples to achieve natural pregnancy. Although assisted reproductive technology (ART) such as in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are popular and effective procedures to treat both female and male factor infertility, they are very expensive and not always successful. Some patients require several attempts of treatment cycles to achieve a pregnancy and bear huge financial and emotional costs in the process.

Information

Type
Chapter
Information
Manual of Sperm Function Testing in Human Assisted Reproduction , pp. 93 - 99
Publisher: Cambridge University Press
Print publication year: 2021

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Book purchase

Temporarily unavailable

References

World Health Organization. (2010) WHO Laboratory Manual for the Examination and Processing of Human Semen, 5th ed. Geneva: The WHO Press.Google Scholar
Liu, DY, Baker, HWG. Tests of human sperm function and fertilization in vitro. Fertil Steril 1992; 58: 465–83.Google Scholar
Liu, DY, Garrett, C, Baker, HWG. Clinical application of sperm-oocyte interaction tests in in vitro fertilization-embryo transfer and intracytoplasmic sperm injection programs. Fertil Steril 2004; 82: 1251–63.Google Scholar
Liu, DY, Baker, HWG. A new test for the assessment of sperm-zona pellucida penetration: relationship with results of other sperm tests and fertilization in vitro. Hum Reprod 1994; 9: 489–96.Google Scholar
Liu, DY, Baker, HWG. Defective sperm-zona pellucida interaction: a major cause of failure of fertilization in clinical in-vitro fertilization. Hum Reprod 2000; 15: 702–8.Google Scholar
Liu, DY, Lopata, A, Johnston, WIH, Baker, HWG. A human sperm-zona pellucida binding test using oocytes that failed to fertilize in vitro. Fertil Steril 1988; 50: 782–8.Google Scholar
Liu, DY, Baker, HWG. A simple method for assessment of the human acrosome reaction of spermatozoa bound to the zona pellucida: lack of relationship with ionophore A23187-induced acrosome reaction. Hum Reprod 1996; 11: 551–5.CrossRefGoogle Scholar
Liu, DY, Baker, HWG. Relationship between the zona pellucida (ZP) and ionophore A23187 induced acrosome reaction and the ability of sperm to penetrate the ZP in men with normal sperm-ZP binding. Fertil Steril 1996; 66: 312–15.CrossRefGoogle ScholarPubMed
Liu, DY, Clarke, GN, Lopata, A, Johnston, WIH, Baker, HWG. A sperm-zona pellucida binding test and in vitro fertilization. Fertil Steril 1989; 52: 281–7.CrossRefGoogle ScholarPubMed
Liu, DY, Baker, HWG. Disordered acrosome reaction of sperm bound to the zona pellucida (ZP): a newly discovered sperm defect causing infertility with reduced sperm-ZP penetration and reduced fertilization in vitro. Hum Reprod 1994; 9: 1694–770.CrossRefGoogle Scholar
Liu, DY, Clarke, GN, Martic, M, Garrett, C, Baker, HWG. Frequency of disordered zona pellucida (ZP)-induced acrosome reaction in infertile men with normal semen analysis and normal sperm-ZP binding. Hum Reprod 2001; 16: 1185–90.CrossRefGoogle Scholar
Liu, DY, Baker, HWG. Disordered zona pellucida induced acrosome reaction and failure of in vitro fertilization in patients with unexplained infertility. Fertil Steril 2003; 79: 74–80.Google Scholar
Liu, DY, Liu, ML, Garrett, C, Baker, HWG. Comparison of the frequency of defective sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction between subfertile men with normal and abnormal semen. Hum Reprod 2007; 22: 1878–84.CrossRefGoogle ScholarPubMed
Yanagimachi, R. Mammalian fertilisation. (1994) In Knobil, E, Neill, J, eds., The Physiology of Reproduction, 2nd ed. New York: Raven Press, pp. 189–317.Google Scholar
Liu, DY, Stewart, T, Baker, HWG. Normal range and variation of the zona pellucida-induced acrosome reaction in fertile men. Fertil Steril 2003; 80: 384–9.Google Scholar
Liu, DY, Bourne, H, Baker, HWG. Fertilization and pregnancy with acrosome intact sperm by intracytoplasmic sperm injection in patients with disordered zona pellucida induced acrosome reaction. Fertil Steril 1995; 64: 116–21.Google Scholar
Liu, DY, Garrett, C, Baker, HWG. Low proportions of spermatozoa can bind to the zona pellucida of human oocytes. Hum Reprod 2003; 18: 2382–9.Google Scholar
Liu, DY, Baker, HWG. Acrosome status and morphology of human sperm bound to the zona pellucida and oolemma determined using oocytes that failed to fertilize in vitro. Hum Reprod 1994; 9: 673–9.CrossRefGoogle Scholar
Liu, DY, Baker, HWG. Human sperm bound to the zona pellucida have normal nuclear chromatin assessed by Acridine Orange fluorescence. Hum Reprod 2007; 22: 1597–1602.Google Scholar
Liu, DY, Liu, ML, Baker, HWG. Defective protein kinase A and C pathways are common causes of disordered zona pellucida-induced (ZP) acrosome reaction in normozoospermic infertile men with normal sperm-ZP binding. Fertil Steril 2013; 99: 86–91.Google Scholar

Accessibility standard: Unknown

Accessibility compliance for the PDF of this book is currently unknown and may be updated in the future.

Save book to Kindle

To save this book to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×