Hostname: page-component-8448b6f56d-wq2xx Total loading time: 0 Render date: 2024-04-23T20:21:01.868Z Has data issue: false hasContentIssue false
  • English
  • Français

Urinary 3-methylhistidine excretion in man: the role of protein-bound and soluble 3-methylhistidine

Published online by Cambridge University Press:  09 March 2007

Gabor Huszar ,
George Golenwsky ,
John Maiocco  and
Edward Davis
Gabor Huszar
Affiliation:
Departments of Obstetrics and Gynecology and Pediatrics, Yale University School of Medicine, 333 Cedar Street, PO Box 3333, New Haven, CT 06510, USA
George Golenwsky
Affiliation:
Departments of Obstetrics and Gynecology and Pediatrics, Yale University School of Medicine, 333 Cedar Street, PO Box 3333, New Haven, CT 06510, USA
John Maiocco
Affiliation:
Departments of Obstetrics and Gynecology and Pediatrics, Yale University School of Medicine, 333 Cedar Street, PO Box 3333, New Haven, CT 06510, USA
Edward Davis
Affiliation:
Departments of Obstetrics and Gynecology and Pediatrics, Yale University School of Medicine, 333 Cedar Street, PO Box 3333, New Haven, CT 06510, USA
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. The influence of dietary meat and meat stock intake on urinary excretion of 3-methylhistidine (3MH) was examined in human adults.

2. In the absence of 3MH ingestion for 48 h, the study subjects adjusted to an intrinsic urinary 3MH: creatinine value. If the meat and meat stock-free diet was maintained on subsequent days, only minute diurnal variations occurred, and the values of random urine samples during the day were representative of the 24 h 3MH: creatinine value.

3. The mean 3MH: creatinine value (SD) for a group of adults (n 7) was 0·105 ± 0·023 (μmol of 3MH/mg creatinine), which is approximately 35% lower than the corresponding value in healthy growing infants (0·148 ± 0·039) (Seashore et al. 1981).

4. Ingestion of meat soup and meat causes different patterns of urinary excretion of 3MH which are consistent with the finding that meat extracts, such as soup and stock, contain considerable amounts of 3MH. The 3MH contents of beef, chicken and turkey were 3·8 ± 0·15, 3·0 ± 0·09 and 2·3 ± 0·29 μmol/g dry wt meat respectively. All three meats contained a water-soluble 3MH-fraction (% total 3MH: beef 8, chicken 21, turkey 23). Amino acid analysis of the soluble fraction with or without hydrochloric acid hydrolysis demonstrated free 3MH in chicken and turkey (5·2 and 2·8% of the total respectively) but not in beef.

5. Patients undergoing urinary 3MH measurements should maintain a diet that is free not only of solid meats, but also of meat stock. The ingestion of commercial food products (e.g. frozen or canned meals, sauces, pizza, etc.) may impair the validity of such measurements because of their meat-stock content.

6. A dietary regimen is presented which is based on a shorter 12 h urine collection. The shorter collection time is satisfactory in the light of the steady rate of 3MH-excretion after 2 d of a diet free of meat and meat stock.

Type
Paper of diract relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 49 , Issue 3 , May 1983 , pp. 287 - 294
Copyright
Copyright © The Nutrition Society 1983

References

Bilmazes, C., Uauy, R., Haverberg, L. N., Munro, H. N. & Young, V. R. (1978). Metabolism 27, 525.CrossRefGoogle Scholar
Block, W. D., Hubbard, R. W. & Steele, B. F. (1965). Nutrition 85, 419.Google Scholar
Elia, M., Carter, A., Bacon, S., Winearls, C. G. & Smith, R. (1981). Br. med. J. 282, 351.CrossRefGoogle Scholar
Elzinga, M., Collins, J. H., Kuehl, W. M. & Adelstein, R. S. (1973). Proc. natn Acad. Sci., USA 70, 2687.CrossRefGoogle Scholar
Graystone, J. E. (1968). In Human Growth, p. 182 [Cheek, D. B., editor]. Philadelphia: Lea & Febiger.Google Scholar
Griggs, R. C., Moxley, R. T. III & Forbes, G. B. (1980). Neurology 30, 1262.CrossRefGoogle Scholar
Gross, I., Holbrook, I. B. & Irving, M. H. (1978). Br. J. Surg. 65, 663.CrossRefGoogle Scholar
Harris, C. I. (1981). Biochem. J. 194, 1011.CrossRefGoogle Scholar
Harris, C. I. & Milne, G. (1980). Biochem. Soc. Trans. 8, 552.CrossRefGoogle Scholar
Harris, C. I. & Milne, G. (1981). Br. J. Nutr. 45, 423.CrossRefGoogle Scholar
Hillgartner, F. B., Williams, A. S., Flanders, J. A., Morin, D. & Hansen, R. J. (1981). Biochemistry 196, 591.Google Scholar
Huszar, G. (1972).Natur., New Biol. 240, 260.CrossRefGoogle Scholar
Huszar, G. & Elzinga, M. (1971). Biochemistry, 10, 229.CrossRefGoogle Scholar
Huszar, G. & Golenwsky, G. (1978). A. Mtg Soc. Gynec. Invest. Abstr. 25.Google Scholar
Huszar, G., Koivisto, V., Davis, E. M. & Felig, P. (1982). Metabolism 31, 188.CrossRefGoogle Scholar
Jaffe, M. (1886). Zeitschr Physiol. Chem. 10, 391.Google Scholar
Long, C. L., Birkhahn, R. H., Geiger, J. W., Betts, J. E., Schiller, W. R. & Blakemore, W. S. (1981). Am. J. clin. Nutr. 30, 756.Google Scholar
Long, C. L., Birkhahn, R. H., Geiger, J. W. & Blakemore, W. S. (1981). Am. J. clin. Nutr. 34, 1087.CrossRefGoogle Scholar
Long, C. L., Haverberg, L. N., Young, V. R., Kinney, J. M. & Munro, H. N. (1975). Metabolism 24, 929.CrossRefGoogle ScholarPubMed
Lukaski, H. C., Mendez, J., Buskirk, E. R. & Cohn, S. H. (1981). J. Am. Physiol. 302.Google Scholar
McKeran, R. O., Halliday, D. & Purkiss, P. (1978). Clin. Sci. Mol. Med. 54, 471.Google Scholar
Millward, D. J., Bates, P. C., Grimble, G. K. & Brown, J. G. (1980). Biochem. J. 190, 225.CrossRefGoogle Scholar
Seashore, J. H., Huszar, G. & Davis, E. M. (1981). Metabolism 30, 959.CrossRefGoogle Scholar
Tomas, F. M., Ballard, F. J. & Pope, L. M. (1979). Clin. Sci. 56, 341.CrossRefGoogle Scholar
Wannemacher, R. W. J., Dinterman, R. E., Pekarek, R. S., Bartellom, P. J. & Beisel, W. R. (1975). Am. J. clin. Nutr. 28, 110.CrossRefGoogle Scholar
Winterer, J., Bistrian, B. R., Bilmazes, C., Blackburn, G. L. & Young, V. R. (1980). Metabolism 29, 575.CrossRefGoogle Scholar
Young, V. R., Alexis, S. D., Baliga, B. S., Munro, H. N. & Muecke, W. (1972). J. biol. Chem. 217, 3592.CrossRefGoogle Scholar
Young, V. R., Haverberg, L. N., Bilmazes, C. & Munro, H. N. (1973). Metabolism 22, 1429.CrossRefGoogle Scholar
Young, V. R. & Munro, H. N. (1978). Fedn Proc. Fedn Am. Socs exp. Biol. 37, 2291.Google Scholar