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A single nucleotide polymorphism in the promoter region of river buffalo stearoyl CoA desaturase gene (SCD) is associated with milk yield

Published online by Cambridge University Press:  20 September 2012

Alfredo Pauciullo ,
Gianfranco Cosenza ,
Roberto Steri ,
Angelo Coletta ,
Antonio La Battaglia ,
Dino Di Berardino ,
Nicolò P P Macciotta  and
Luigi Ramunno
Alfredo Pauciullo*
Affiliation:
Department of Soil, Plant, Environment and Animal Production Science, University of Naples ‘Federico II’, Portici (NA), Italy
Gianfranco Cosenza*
Affiliation:
Department of Soil, Plant, Environment and Animal Production Science, University of Naples ‘Federico II’, Portici (NA), Italy
Roberto Steri
Affiliation:
Department of Agricultural Sciences, University of Sassari, Sassari, Italy
Angelo Coletta
Affiliation:
Associazione Nazionale Allevatori Specie Bufalina, Località Centurano, Caserta, Italy
Antonio La Battaglia
Affiliation:
Comunità Montana Alto Agri, Villa D'Agri di Marsicovetere (PZ), Italy
Dino Di Berardino
Affiliation:
Department of Soil, Plant, Environment and Animal Production Science, University of Naples ‘Federico II’, Portici (NA), Italy
Nicolò P P Macciotta
Affiliation:
Department of Agricultural Sciences, University of Sassari, Sassari, Italy
Luigi Ramunno
Affiliation:
Department of Soil, Plant, Environment and Animal Production Science, University of Naples ‘Federico II’, Portici (NA), Italy
*
*For correspondence; e-mail: apauciul@unina.it; giacosen@unina.it
*For correspondence; e-mail: apauciul@unina.it; giacosen@unina.it
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Abstract

An association study between the milk yield trait and the stearoyl-CoA desaturase (SCD) polymorphism (g.133A > C) in Italian Mediterranean river buffalo was carried out. A full characterization of the river buffalo SCD promoter region was presented. Genotyping information was provided and a quick method for allelic discrimination was developed. The frequency of the C allele was 0·16. Test-day (TD) records (43 510) of milk production belonging to 226 lactations of 169 buffalo cows were analysed with a mixed linear model in order to estimate the effect of g.133A > C genotype, as well as the effect of parity and calving season. The SCD genotype was significantly associated with milk yield (P = 0·02). The genotype AC showed an over-dominance effect with an average daily milk yield approximately 2 kg/d higher than CC buffaloes. Such a difference represents about 28% more milk/d. The effect of the genotype was constant across lactation stages. The contribution of SCD genotype (r2SCD) to the total phenotypic variance in milk yield was equal to 0·12. This report is among the first indications of genetic association between a trait of economic importance in river buffalo. Although such results need to be confirmed with large-scale studies in the same and other buffalo populations, they might offer useful indications for the application of MAS programmes in river buffalo and in the future they might be of great economic interest for the river buffalo dairy industry.

Keywords

Mediterranean river buffalostearoyl CoA desaturase gene (SCD)milk yieldmarker assisted selection
Type
Research Article
Information
Journal of Dairy Research , Volume 79 , Issue 4 , November 2012 , pp. 429 - 435
Copyright
Copyright © Proprietors of Journal of Dairy Research 2012

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