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Rapid detection of cow milk adulteration/contamination in goat milk by a lateral flow colloidal gold immunoassay strip

Published online by Cambridge University Press:  07 February 2019

Bochao Liu ,
Jinhong Si ,
Fang Zhao ,
Qi Wang ,
Yu Wang ,
Jinfeng Li ,
Chengyao Li  and
Tingting Li
Bochao Liu
Affiliation:
Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China
Jinhong Si
Affiliation:
Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China
Fang Zhao
Affiliation:
Shenzhen Key Research Laboratory of Detection Technology R&D on Food Safety, Technical Centre for Food Inspection and Quarantine, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, China Shenzhen Academy of Inspection and Quarantine, Shenzhen, China
Qi Wang
Affiliation:
Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China
Yu Wang
Affiliation:
Nanjing Entry-exit Inspection and Quarantine Bureau, Nanjing, China
Jinfeng Li
Affiliation:
Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China
Chengyao Li
Affiliation:
Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China
Tingting Li*
Affiliation:
Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China Shenzhen Key Research Laboratory of Detection Technology R&D on Food Safety, Technical Centre for Food Inspection and Quarantine, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, China Shenzhen Academy of Inspection and Quarantine, Shenzhen, China Nanjing Entry-exit Inspection and Quarantine Bureau, Nanjing, China School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China
*
Author for correspondence: Tingting Li, Email: apple-ting-007@163.com
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Abstract

Current available methods to detect cow milk adulteration or accidental contamination of goat milk are both laborious and time consuming. The aim of this technical research communication was to develop a simple, rapid, specific and sensitive method for quantitative detection of cow milk in goat milk. A competitive lateral flow immunoassay (LFIA) strip was developed using a specific monoclonal antibody (mAb) labeled with colloidal gold nanoparticles (GNPs) for specifically binding to cow milk casein. The detection limit of this rapid detection was 0.07% of cow milk in goat milk, providing equal specificity and higher sensitivity when compared with a commercial enzyme-linked immunosorbent assay (ELISA). These result suggest that the established rapid GNPs-LFIA strip could be used for monitoring cow milk adulteration/contamination of goat milk.

Keywords

Colloidal gold nanoparticlescontamination or adulterationcow milkgoat milklateral flow immunoassay strip
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 1 , February 2019 , pp. 94 - 97
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

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Footnotes

†

These authors contributed equally to this work.

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