Hostname: page-component-7c8c6479df-p566r Total loading time: 0 Render date: 2024-03-28T12:37:10.830Z Has data issue: false hasContentIssue false

Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis

Published online by Cambridge University Press:  22 February 2007

A. Ito*
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
M.I. Putra
Affiliation:
Animal Clinic of Hom-Hom, Veterinary Unit of Jayawijaya District, Irian Jaya, Indonesia
R. Subahar
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
M.O. Sato
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
M. Okamoto
Affiliation:
Department of Experimental Animal Sciences, Faculty of Veterinary Medicine, School of Agriculture, Tottori, Tottori University, Japan
Y. Sako
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
M. Nakao
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
H. Yamasaki
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
K. Nakaya
Affiliation:
Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa, Japan
P.S. Craig
Affiliation:
Cestode Zoonoses Research Group, Biosciences Research Institute, University of Salford, Salford, M5 4WT, UK
S.S. Margono
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
*
*Fax: +81 166 68 2429 E-mail: akiraito@asahikawa-med.ac.jp

Abstract

Serology (ELISA and immunoblot) using native glycoproteins, affinity purified glycoproteins, and a recombinant antigen is known to be highly specific to Taenia solium cysticercosis in humans and pigs. These techniques were applied for dogs in the highly endemic area of cysticercosis in Papua (Irian Jaya), Indonesia. Analysis of dog sera by both ELISA and immunoblot revealed 7 of 64 dogs were highly positive. Examination of two sero-positive dogs revealed cysticerci of T. solium in the brain and heart of these dogs. Mitochondrial DNA analysis confirmed that they were the same as T. solium previously confirmed from pigs and biopsies from local people from Irian Jaya. It is suggested that the life cycle of T. solium may be completed not only between humans and pigs but also between humans and dogs.

Type
Research Article
Copyright
Cambridge University Press 2002

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bowles, J. & McManus, D.P. (1994) Genetic characterization of the Asian Taenia, a newly described taeniid cestode of humans. American Journal of Tropical Medicine and Hygiene 50, 3344.CrossRefGoogle ScholarPubMed
Ito, A., Plancarte, A., Ma, L., Kong, Y., Flisser, A., Cho, S.Y., Liu, Y.H., Kamhawi, S., Lightowlers, M.W. & Schantz, P.M. (1998) Novel antigens for neurocysticercosis: simple method for preparation and evaluation for serodiagnosis. American Journal of Tropical Medicine and Hygiene 59, 291294.CrossRefGoogle ScholarPubMed
Ito, A., Plancarte, A., Nakao, M., Nakaya, K., Ikejima, T., Piao, Z.X., Kanazawa, T. & Margono, S.S. (1999) ELISA and immunoblot using purified glycoproteins for serodiagnosis of cysticercosis in pigs naturally infected with Taenia solium. Journal of Helminthology 73, 363365.CrossRefGoogle ScholarPubMed
Ito, A., Sako, Y., Nakao, M. & Nakaya, K. (2002a) Neurocysticercosis in Asia: serology/seroepidemiology in humans and pigs. pp. 2531 in Craig, P.S. & Pawlowski, Z. (Eds) Cestode zoonoses: echinococcosis and cysticercosis – anemergentandglobalproblem. Amsterdam, IOS Press.Google Scholar
Ito, A., Nakao, M., Okamoto, M., Sako, Y. & Yamasaki, H. (2002b) Mitochondrial DNA of Taenia solium: from basic to applied science. Chapter 5 in Singh, G. & Prabhakar, S. (Eds) Taenia solium cysticercosis: from basic to clinical science. Wallingford, Oxon, CABI Publishing.Google Scholar
McManus, D.P. (1997) Molecular genetic variation in Echinococcus and Taenia: an update. Southeast Asian Journal of Tropical Medicine and Public Health 28 (Suppl 1), 110116.Google ScholarPubMed
Meijer, W.C.P. (1933) Extracten unit de maand – en jaarverslagen der gouvernements en provinciale veeartsen. No. 62, I. Cysticercosis. II. Trichinosis. Nederlandsch-Indische Bladen voor Diergeneeskunde en Dierenteelt 14 (3), 165175 (see Helminthological Abstracts 1933, 2, 88).Google Scholar
Nakao, M., Okamoto, M., Sako, Y., Yamasaki, H., Nakaya, K. & Ito, A. (2002) A phylogenetic hypothesis for the distribution of two genotypes of the pig tapeworm Taenia solium worldwide. Parasitology 124, 657662.CrossRefGoogle ScholarPubMed
Okamoto, M., Bessho, Y., Kamiya, M., Kurosawa, T. & Horii, T. (1995) Phylogenetic relationships within Taenia taeniaeformis variants and other taeniid cestodes inferred from the nucleotide sequences of the cytochrome c oxidase subunit I gene. Parasitology Research 81, 451458.CrossRefGoogle ScholarPubMed
Okamoto, M., Nakao, M., Sako, Y. & Ito, A. (2001) Molecular variation of Taenia solium in the world. Southeast Asian Journal of Tropical Medicine and Public Health 32 (Suppl 2), 9093.Google ScholarPubMed
Okolo, M.I.O. (1986) Observations on Cysticercus cellulosae in the flesh of rural dogs. International Journal of Zoonoses 13, 286289.Google ScholarPubMed
Sako, Y., Nakao, M., Ikejima, T., Piao, Z.X., Nakaya, K. & Ito, A. (2000) Molecular characterization and diagnostic value of Taenia solium low-molecular-weight antigen genes. Journal of Clinical Microbiology 38, 44394444.CrossRefGoogle ScholarPubMed
Simanjuntak, G.M., Margono, S.S., Okamoto, M. & Ito, A. (1997) Taeniasis/cysticercosis in Indonesia as an emerging disease. Parasitology Today 13, 321323.CrossRefGoogle Scholar
Subahar, R., Hamid, A., Purba, W., Wandra, T., Karma, C., Sako, Y., Margono, S.S., Craig, P.S. & Ito, A. (2001) Taenia solium infection in Irian Jaya (West Papua), Indonesia: a pilot serological survey of human and porcine cysticercosis in Jayawijaya District. Transactions of the Royal Society of Tropical Medicine and Hygiene 95, 388390.CrossRefGoogle ScholarPubMed
Theis, J.H., Cleary, M., Syvanen, M., Gilson, A., Swift, P., Banks, J. & Johnson, E. (1996) DNA-confirmed Taenia solium cysticercosis in black bears (Ursus americanus ) from California. American Journal of Tropical Medicine and Hygiene 55, 456458.CrossRefGoogle ScholarPubMed
Verster, A. (1967) Redescription of Taenia solium Linnaeus, 1758 and Taenia saginata Goeze, 1782. Zeitschrift für Parasitenkunde 29, 313328.CrossRefGoogle ScholarPubMed
Wandra, T., Subahar, R., Simanjuntak, G.M., Margono, S.S., Suroso, T., Okamoto, M., Nakao, M., Sako, Y., Nakaya, K., Schantz, P.M. & Ito, A. (2000) Resurgence of cases of epileptic seizures and burns associated with cysticercosis in Assologaima, Jayawijaya, Irian Jaya, Indonesia, 1991–95. Transactions of the Royal Society of Tropical Medicine and Hygiene 94, 4650.CrossRefGoogle ScholarPubMed