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The TcTASV proteins are novel promising antigens to detect active Trypanosoma cruzi infection in dogs

Published online by Cambridge University Press:  13 May 2016

N. FLORIDIA-YAPUR ,
M. MONJE RUMI ,
P. RAGONE ,
J. J. LAUTHIER ,
N. TOMASINI ,
A. ALBERTI D'AMATO ,
P. DIOSQUE ,
R. CIMINO ,
J. D. MARCO  and
P. BARROSO
...Show all authors
N. FLORIDIA-YAPUR
Affiliation:
Cátedra de Química Biológica, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina Instituto de Investigaciones de Enfermedades Tropicales (IIET), Sede Regional Orán, Universidad Nacional de Salta, Alvarado 751, Orán-Salta (4530), Argentina
M. MONJE RUMI
Affiliation:
Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
P. RAGONE
Affiliation:
Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
J. J. LAUTHIER
Affiliation:
Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
N. TOMASINI
Affiliation:
Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
A. ALBERTI D'AMATO
Affiliation:
Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
P. DIOSQUE
Affiliation:
Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
R. CIMINO
Affiliation:
Cátedra de Química Biológica, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina Instituto de Investigaciones de Enfermedades Tropicales (IIET), Sede Regional Orán, Universidad Nacional de Salta, Alvarado 751, Orán-Salta (4530), Argentina
J. D. MARCO
Affiliation:
Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
P. BARROSO
Affiliation:
Instituto de Patología Experimental (IPE-CONICET), Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
D. O. SANCHEZ
Affiliation:
Instituto de Investigaciones Biotecnológicas “Dr. Rodolfo A. Ugalde” (IIB-INTECH), Universidad Nacional de San Martín, UNSAM-CONICET, Av. 25 de Mayo, Partido de San Martín, Buenos Aires (1650), Argentina
J. R. NASSER
Affiliation:
Cátedra de Química Biológica, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina Instituto de Investigaciones de Enfermedades Tropicales (IIET), Sede Regional Orán, Universidad Nacional de Salta, Alvarado 751, Orán-Salta (4530), Argentina
V. TEKIEL*
Affiliation:
Instituto de Investigaciones Biotecnológicas “Dr. Rodolfo A. Ugalde” (IIB-INTECH), Universidad Nacional de San Martín, UNSAM-CONICET, Av. 25 de Mayo, Partido de San Martín, Buenos Aires (1650), Argentina
*
*Corresponding author: Instituto de Investigaciones Biotecnológicas “Dr. Rodolfo A. Ugalde” (IIB-INTECH), Universidad Nacional de San Martín, UNSAM-CONICET, Av. 25 de Mayo y Francia, Partido de San Martín, Buenos Aires (1650), Argentina. E-mail: valet@iib.unsam.edu.ar or vtekiel@gmail.com
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Summary

In regions where Chagas disease is endemic, canine Trypanosoma cruzi infection is highly correlated with the risk of transmission of the parasite to humans. Herein we evaluated the novel TcTASV protein family (subfamilies A, B, C), differentially expressed in bloodstream trypomastigotes, for the detection of naturally infected dogs. A gene of each TcTASV subfamily was cloned and expressed. Indirect enzyme-linked immunosorbent assays (ELISA) were developed using recombinant antigens individually or mixed together. Our results showed that dogs with active T. cruzi infection differentially reacted against the TcTASV-C subfamily. The use of both TcTASV-C plus TcTASV-A proteins (Mix A+C-ELISA) enhanced the reactivity of sera from dogs with active infection, detecting 94% of the evaluated samples. These findings agree with our previous observations, where the infected animals exhibited a quick anti-TcTASV-C antibody response, coincident with the beginning of parasitaemia, in a murine model of the disease. Results obtained in the present work prove that the Mix A+C-ELISA is a specific, simple and cheap technique to be applied in endemic areas in screening studies. The Mix A+C-ELISA could help to differentially detect canine hosts with active infection and therefore with high impact in the risk of transmission to humans.

Keywords

Trypanosoma cruzidogsTcTASV antigensimmunodiagnosis
Type
Research Article
Information
Parasitology , Volume 143 , Issue 11 , September 2016 , pp. 1382 - 1389
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Copyright
Copyright © Cambridge University Press 2016 

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