This study investigates screening practices for antimicrobial-resistant organisms (AROs) in seventy-five hospitals participating in the Canadian Nosocomial Infection Surveillance Program (CNISP). Screening practices varied with widespread MRSA screening, selective carbapenemase-producing organisms (CPO) screening, and limited vancomycin-resistant Enterococcus (VRE) screening. These findings may help interpret ARO rates within CNISP hospitals and inform screening practices.