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Impulsivity is a multidimensional trait associated with substance use disorders (SUDs), but the relationship between distinct impulsivity facets and stages of substance use involvement remains unclear.
Methods
We used genomic structural equation modeling and genome-wide association studies (N = 79,729–903,147) to examine the latent genetic architecture of nine impulsivity traits and seven substance use (SU) and SUD traits.
Results
We found that the SU and SUD factors were strongly genetically inter-correlated (rG=0.77) but their associations with impulsivity facets differed. Lack of premeditation, negative and positive urgency were equally positively genetically correlated with both the SU (rG=.0.30–0.50) and SUD (rG=0.38–0.46) factors; sensation seeking was more strongly genetically correlated with the SU factor (rG=0.27 versus rG=0.10); delay discounting was more strongly genetically correlated with the SUD factor (rG=0.31 versus rG=0.21); and lack of perseverance was only weakly genetically correlated with the SU factor (rG=0.10). After controlling for the genetic correlation between SU/SUD, we found that lack of premeditation was independently genetically associated with both the SU (β=0.42) and SUD factors (β=0.21); sensation seeking and positive urgency were independently genetically associated with the SU factor (β=0.48, β=0.33, respectively); and negative urgency and delay discounting were independently genetically associated with the SUD factor (β=0.33, β=0.36, respectively).
Conclusions
Our findings show that specific impulsivity facets confer risk for distinct stages of substance use involvement, with potential implications for SUDs prevention and treatment.
We investigate experimentally the planar paths displayed by cylinders falling freely in a thin-gap cell containing liquid at rest, by varying the elongation ratio and the Archimedes number of the cylinders, and the solid-to-fluid density ratio. In the investigated conditions, the oscillatory falling motion features two main characteristics: the mean fall velocity $\overline {u_v}$ does not scale with the gravitational velocity, which overestimates $\overline {u_v}$ and is unable to capture the influence of the density ratio on it; and high-amplitude oscillations of the order of $\overline {u_v}$ are observed for both translational and rotational velocities. To model the body behaviour, we propose a force balance, including proper and added inertia terms, the buoyancy force and vortical contributions accounting for the production of vorticity at the body surface and its interaction with the cell walls. Averaging the equations over a temporal period provides a mean force balance that governs the mean fall velocity of the cylinder, revealing that the coupling between the translational and rotational velocity components induces a mean upward inertial force responsible for the decrease of $\overline {u_v}$. This mean force balance also provides a normalization for the frequency of oscillation of the cylinder in agreement with experimental measurements. We then consider the instantaneous force balance experienced by the body, and propose three contributions for the modelling of the vortical force. These can be interpreted as drag, lift and history forces, and their dependence on the control parameters is adjusted on the basis of the experimental measurements.
This study explores the relationship between maternal working hours and a child's emotional well-being using data from the UK Millennium Cohort Study. Child well-being is assessed through self-reported happiness and a well-being index that includes concerns, temperament, bullying, and behaviour. Results show a positive association between maternal employment and child well-being, supported by factor analysis combining child, mother, and teacher reports. The association remains consistent across income levels and is unaffected by commuting time or cohabitation status. These findings highlight the importance of maternal employment and contextual factors in shaping child well-being.
The use of long-acting treatments is a common clinical practice in psychiatry. No disease insight and the risk of treatment discontinuation in a significant portion of our patients, increase the demand for psychiatric emergency and hospital admissions. Treatment adherence must be facilitated, taking into account possible side effects and patient´s subjective satisfaction.
Objectives
-Evaluate the type of long-acting intramuscular treatment in selected patients. -Evaluate the differences in treatment satisfaction between different types of long-acting intramuscular treatments as well as frequency of psychiatric emergency and hospital admissions in the last year.
Methods
We select patients with different severe mental disorders who stay in a Medium Stay Unit, Sociosanitary Community Residence, Supervise house and Residence for the elderly in Albacete (Spain); all of them, with intramuscular neuroleptic treatment (zuclopenthixol dihydrochloride, aripiprazole long acting, palmitate paliperidone monthly, 3-monthly and 6-monthly) at least 1 year.
We evaluate their sociodemographic characteristics, the satisfaction questionnaire with the treatment (TSQM-9) and the rate of psychiatric emergencies and admissions after current intramuscular treatment in last year.
Results
We have selected 57 patients with an average age of 45.86. 78.94% with a diagnosis of schizophrenia, 12.28% with schizoaffective disorder, 5.26% bipolar disorder and 3.5% unspecified psychotic disorder.
We can see in the graphics below that the longer duration of the intramuscular treatment, the greater satisfaction in all the items of the TSQM-9 questionnaire.
31% of the patients with zuclopenthixol dihydrochloride treatment, have gone to psychiatric emergencies and 28% of psychiatric admissions in the last year.18% of the patients with aripiprazole long acting, 17% with paliperidone palmitate long acting-monthly and 12% de 3-monthly have gone to psychiatric emergencies and 15%, 12% and 12% needed psychiatric admissions respectively. Patients with palmitate long acting-monthly have not emergencies or psychiatric admissions in the last year.
Image:
Image 2:
Image 3:
Conclusions
- The longer long acting of the intramuscular treatments, the better patient satisfaction.
- With the longer duration treatment (Palmitate paliperidone LD 6 month), we have lower psychiatric emergencies and hospital admissions.
Covid-19 has profoundly altered festive practices all around the world. Public health regulations have imposed restrictions on public celebrations, compromising social rhythm and community relationships, and calling the real and symbolic objects connected with the lockdown period into question (Appadurai 1996). The “normality” of mass public rituals has had to be suspended due to restrictions connected with lockdown, social distancing, and new forms of social control (Mansilla 2020). This has translated into feelings of social frustration and community mourning caused by the suspension of festivities (Bindi 2021). Faced with the impossibility of performing their collective practices, communities have reacted in different ways, from cancelling festivals to holding them using alternative smaller or digital formulas, and in some cases conflicts of greater or lesser intensity have appeared. One could even say that the pandemic has resulted in many communities reacting with an attitude of resilience with new forms of use and negotiation of intangible heritage, stressing its political nature.
This chapter examines the social effects, debates, and conflicts experienced in festivals in Catalonia through ethnographic research performed from a dual perspective: on the one hand, online analysis of the debates and alternatives used to hold festivities; and, on the other hand, an ethnographic analysis of some cases studies in Catalonia through participant observation and interviews with people connected with these celebrations. Examination of the public actions performed during this short period of time is fertile ground to analyze the changes made to the concept of festivities, through a process of reconfiguration of traditions (Testa and Isnart 2020).
Our objective is to use the case of Catalonia to set out the theoretical scope and importance of processes to recreate heritage in relation to festive practices. We aim to show how festive practices are changeable and how human activities establish and manipulate their own differentiations and purposes in a ritual context (Bell 2007). From this point of view, we have formulated the hypothesis that the processes of change in festivals adopted in the past year have come at the expense of conflicts between the logic of lockdown and the logic of festivities. In this sense, festive practices have sometimes been stages not just for cohesion and solidarity but also the dramatization of social tensions.
THE IMPACT OF COVID-19 ON FESTIVITIES: FROM ALTERING COMMUNITY RHYTHMS TO FORMULATING NEW RITUAL PRACTICES
All festivals undergo constant processes of change and adaptation.
Mirror exposure therapies (MET) have been proposed to reduce symptomatology in patients with Anorexia Nervosa. However, most MET protocols or related studies do not specify the patients’ distance to the mirror, or when they do so, such a distance may differ significantly (from 0,5 to 3 meters). Such modifications of mirror positioning could imply variations in patients’ fixation patterns on different body parts (i.e., attentional bias between weight-related and non-weight related body parts), since previous studies shown that dissociated neural systems (either in left or right cerebral hemispheres) are involved in the attentional patterns and scanning strategies depending on the distance (i.e., in near and far space). Furthermore, as the body-related attentional bias (AB) has been shown to be a part of the maintenance mechanism of AN symptomatology, any modification of attentional patterns due to mirror’s distance variations may influence the efficacy of MET.
Objectives
This study aims to use Virtual Reality (VR) and Eye-Tracking (ET) technologies to precisely analyse the effect of the distance to the mirror on the attentional patterns.
Methods
137 female college students were immersed in a VR environment in which they could look in the mirror at their respective avatars created from the measurements and photos of their real bodies. The mirror was positioned at 3.30m in front of the participants in “group 1” (n1 = 54), and at 1.54m in front of the participants in “group 2” (n2 = 83). Eye-Tracking feature and OGAMA software (Freie Universität, Berlin, Germany) were used to record and process the visual attentional pattern of each participant, during a 30-second free viewing task at her avatar. Complete Fixation Time (CFT) was assessed as the fixation time difference between weight- and non-weight- related body parts, defined from the weight scale of the PASTAS questionnaire. Independent Sample t-Test was conducted to analyse CFT mean difference between both groups.
Results
Independent Samples t-Test shows statistically significant CFT mean difference (F (1, 135) = 1.571, p < 0.001, 95% IC [1717; 5581]) between both groups. While fixation pattern of the group positioned further to the mirror (group 1) was more focused on weight-related body parts (CFT mean = 2282ms, SD = 809), the fixation pattern of the group positioned closer to the mirror (group 2) was more focused on non-weight-related body parts (CFT mean = -1367ms, SD = 587).
Conclusions
This study shows new opportunities to use VR and ET technologies to precisely analyse the variations of fixation patterns as a function of mirror position in MET. Such information may contribute to adapt and develop new MET’s protocols for AN patients, optimizing the distance to the mirror. It also underscores the importance of specifying the distance to the mirror in MET-related studies to improve replicability.
We investigate experimentally the coalescence cascade process for a confined swarm of deformable bubbles immersed in a bidimensional vertical cell filled with water. For different gas volume fractions, air bubbles of size $D_0$ larger than the cell thickness are injected at the bottom of the cell. The bubbles swarms transformation is explored using high-speed visualizations. The time evolution of each bubble in the swarm is determined using a specifically developed algorithm, enabling bubble tracking and coalescence detection. We determine the evolution of the bubble size distribution downstream from the injection point, and show that the stages of the coalescence cascade are characterized by the diameter, $D_{V90}$, representative of the largest bubbles. The collision frequency of pairs of bubbles of sizes $D_k$ and $D_{k'}$, $h(D_k, D_{k'})$, and their coalescence efficiency, $\lambda$, are obtained from the experiments. The efficiency is nearly constant, independently of the bubble sizes and of the gas volume fraction. Concerning collision frequency, our results reveal the existence of two different coalescence regimes depending on the capability of the bubbles to deform. Models describing $h(D_k, D_{k'})$ for both regimes are provided. They take into account the specific response of the bubble pair, which depends on the reduced diameter $D_p = 2 D_k D_{k'} / (D_k + D_{k'})$, to the global swarm-induced agitation governed by $D_{V90}$ and the gas volume fraction. In the first regime, occurring for smaller $D_p$, bubbles are brought together by agitation and rapidly coalesce, while for sufficiently large $D_p$, both bubbles are able to deform and spend more time adapting mutually their shapes before coalescing.
The Research Domain Criteria (RDoC) approach proposes a novel psychiatric nosology using transdiagnostic dimensional mechanistic constructs. One candidate RDoC indicator is delay discounting (DD), a behavioral economic measure of impulsivity, based predominantly on studies examining DD and individual conditions. The current study sought to evaluate the transdiagnostic significance of DD in relation to several psychiatric conditions concurrently.
Methods
Participants were 1388 community adults (18–65) who completed an in-person assessment, including measures of DD, substance use, depression, anxiety, posttraumatic stress disorder, and attention-deficit hyperactivity disorder (ADHD). Relations between DD and psychopathology were examined with three strategies: first, examining differences by individual condition using clinical cut-offs; second, examining DD in relation to latent psychopathology variables via principal components analysis (PCA); and third, examining DD and all psychopathology simultaneously via structural equation modeling (SEM).
Results
Individual analyses revealed elevations in DD were present in participants screening positive for multiple substance use disorders (tobacco, cannabis, and drug use disorder), ADHD, major depressive disorder (MDD), and an anxiety disorder (ps < 0.05–0.001). The PCA produced two latent components (substance involvement v. the other mental health indicators) and DD was significantly associated with both (ps < 0.001). In the SEM, unique significant positive associations were observed between the DD latent variable and tobacco, cannabis, and MDD (ps < 0.05–0.001).
Conclusions
These results provide some support for DD as a transdiagnostic indicator, but also suggest that studies of individual syndromes may include confounding via comorbidities. Further systematic investigation of DD as an RDoC indicator is warranted.
Electroconvulsive therapy is a highly effective treatment for severe psychopharmacological resistant patients but it is also a procedure that involves open airway management and has been considered as an aerosol generating procedure. The COVID-19 pandemic, has resulted in reduction in ECT services internationally. The COVID-19 pandemic has dramatically and rapidly transformed hospitals in heavily affected areas, decreasing mental health services. The need to locate critical patients in spaces intended for anesthesia, where we usually administered ECT, has forced us to decrease the number of procedures and be highly selective. In the same way, continuation and maintenance ECT (m-ECT) have also been dramatically reduced. The risk of contagion urged us to develop a protocol involving other areas of the hospital
Objectives
To create a safe circuit from admission to the hospital to the ECT including emergency room and psychiatric Ward
Methods
Review of the tliterature and published protocols Workshops with Preventive Medicine, Anaesthesia and Emergency Service to elaborate a protocol Submission of the protocol to Management of the Hospital
Results
The protocol (Figure 1) began with the screening for COVID-19 in every patient. If the PCR was (+) the patient was not excluded. We moved treatment from the PACU into the OR and if a patient tested positive It was determined that the ECT was administered in the OR
That was provided with negative pressure. Circuits were established within the Psychiatric Ward and in the areas of the hospital involved to reduce risks and patients remained isolated until negative test was confirmed The number of persons present in the treatment room was kept to the absolute minimum required and appropriate personal protective equipment was used, as prescribed by the WHO
Conclusions
We must keep in mind treating the most vulnerable of our patients. ECT should be seen as an essential medical procedure and made available
A considerable percentage of Health Care Workers (HCW) have experienced psychological distress during the COVID-19 pandemic. Data from previous pandemics suggest that HCW might develop psychiatric disorders. Psychosocial and workplace measures can improve mental wellbeing of the MHW. As part of the program of the Hospital to give support to the HCW, five support weekly open dynamic groups have been carried out with HCW from the COVID Areas of our Hospital including the ICU
Objectives
Identify recurrent contents in the group that express areas ofconcern Identify HCW in risk of develop a psuchiatric disorder and refer them to their apropiate level o
Methods
The sessions were carried out in a freely open group and the contents expressed in the sessions were recorded and analyzed ina narrative way. Special attention was given to the the more stresfull activities identified, to Signs of overload and to the ability to seek relief, as well as signs of disruption of personal life outside of work. Four sessions of 90 minutes, with staff of the same area were established and after these four sessions booster sessions was offered through continuity groups to members with need of more long term care as well as individual care.
Results
The recurrent areas identified were Concern about inadequite Personal Protective Equipment Concern about spreading the infection in their own families Need for relief and avoid double turn Uncertainty about the course of the illness Exposure to patients suffering and dying
Conclusions
HCW need nor only psychological support but also pragmatic measures
Moradisaurine captorhinid eureptiles were a successful group of high-fibre herbivores that lived in the arid low latitudes of Pangaea during the Permian. Here we describe a palaeoassemblage from the Permian of Menorca (Balearic Islands, western Mediterranean), consisting of ichnites of small captorhinomorph eureptiles, probably moradisaurines (Hyloidichnus), and parareptiles (cf. Erpetopus), and bones of two different taxa of moradisaurines. The smallest of the two is not diagnostic beyond Moradisaurinae incertae sedis. The largest one, on the other hand, shows characters that are not present in any other known species of moradisaurine (densely ornamented maxillar teeth), and it is therefore described as Balearosaurus bombardensis gen. et sp. nov. Other remains found in the same outcrop are identified as cf. Balearosaurus bombardensis gen. et sp. nov., as they could also belong to the newly described taxon. This species is sister to the moradisaurine from the lower Permian of the neighbouring island of Mallorca, and is also closely related to the North American genus Rothianiscus. This makes it possible to suggest the hypothesis that the Variscan mountains, which separated North America from southern Europe during the Permian, were not a very important palaeobiogeographical barrier to the dispersion of moradisaurines. In fact, mapping all moradisaurine occurrences known so far, it is shown that their distribution area encompassed both sides of the Variscan mountains, essentially being restricted to the arid belt of palaeoequatorial Pangaea, where they probably outcompeted other herbivorous clades until they died out in the late Permian.
Silvia Roig explores the narrative of Aurora Bertrana (1892-1974), an unknown writer today, but a successful and recognized female author in Catalonia and Spain during the 20th century. Aurora Bertrana's works are almost never mentioned in manuals of literature. Her rich, intellectual work has not received the attention it deserves, relegated almost to absolute oblivion. The author reviews and studies twenty-four of Bertrana's novels written in Catalan and Spanish, including: Ariatea (1960), "El pomell de les violes" (MS), L'inefable Philip (MS), La aldea sin hombres (mn.), La madrecitade los cerdos (MS), Entre dos silencis (1958), La ninfa d'argila (1959), Fracás (1966) and La ciutat dels joves: reportatge fantasia (1971). She studies her work, published and unpublished, from a feminist approach, taking into account the intellectual history of Spain and Catalonia. Bertana's strong commitment to social issues reveals her association with the Modernist and Noucentists trends of her time. Bertrana's novels reveal a unique interest in non-Western cultures and lifestyles and her work undertakes controversial topics and socio-cultural issues, while she observes and draws special attention to the situation of women in different circumstances and cultural geographies. This book is therefore anchored on interpretive and theoretical parameters that intersect with consideration of gender, such as travel-and-gender and war-and-gender. Roig uses the work of feminists such as Simone De Beauvoir, Shulamith Firestone, Jelke Boesten, Margaret and PatriceHigonnet, Michelle Zimbalist Rosaldo and Julia Kristeva to help assess Bertrana's engagement with gender and socio-political issues. This approach is particularly well suited for a writer like Bertrana, a Catalan and Republican intellectual woman forced into self-exile during the Spanish Civil War and the dictatorship of Francisco Franco. Silvia Roig is a Faculty Member, BMCC Department of Modern Languages, The City University of New York.
We present the first report of Amblycerus dispar (Sharp) attacking stored almonds [Prunus dulcis (Mill.) D. A. Webb] in Argentina. A summarized diagnosis, illustrations, and photographs of the adult and mature larva are provided to facilitate identification. We performed species distribution models for A. dispar and its main host plant Geoffroea decorticans (Gillies ex Hook. & Arn.) Burkart. We include A. dispar into a previous morphological character matrix and conduct a phylogenetic analysis to infer its phylogenetic position. The evolution of host plant associations of the genus Amblycerus is herein re-analyzed. A. dispar and its main host shows high suitability areas especially in central-west Argentina and Chile, whereas for the USA, high suitability areas were found for the south-western which include the area of almond production in this country. Although the presence of A. dispar in the USA region is very unlikely, we recommend some awareness as other bruchines are present in the area. Although A. dispar is unlikely to become an economically important risk, monitoring for early detection is recommended to avoid productivity loss, especially when the native host is nearby cultivated areas. A. dispar is hypothesized to be the sister species of A. schwarzi Kingsolver. The colonization of a Rosaceae species is a novelty for this genus, being host shifts known as an important factor affecting both natural and agricultural systems.
An outbreak of SARS-CoV2 infection in a Barcelona prison was studied. One hundred and forty-eight inmates and 36 prison staff were evaluated by rt-PCR, and 24.1% (40 prisoners, two health workers and four non-health workers) tested positive. In all, 94.8% of cases were asymptomatic. The inmates were isolated in prison module 4, which was converted into an emergency COVID unit. There were no deaths. Generalised screening and the isolation and evaluation of the people infected were key measures. Symptom-based surveillance must be supplemented by rapid contact-based monitoring in order to avoid asymptomatic spread among prisoners and the community at large.
This article on Old Norse represents a fundamental departure from the previous literature on loaned material by examining multilingual documents written in Medieval Latin rather than in monolingual English, namely the Durham Account Rolls (DAR). The potential of this richer and more complex interplay between languages will be further addressed throughout the article, which assesses the different kinds of evidence available for establishing the relative plausibility for a word being derived from ON. Dance's (2013, 2018, 2019) taxonomy will be discussed and applied to multilingual material for the first time. The article concludes with some notes on the main semantic fields to which ON-derived lexis contributed within the multilingual lexical networks of the DAR.
Compulsory admission procedures of patients with mental disorders vary between countries in Europe. The Ethics Committee of the European Psychiatric Association (EPA) launched a survey on involuntary admission procedures of patients with mental disorders in 40 countries to gather information from all National Psychiatric Associations that are members of the EPA to develop recommendations for improving involuntary admission processes and promote voluntary care.
Methods.
The survey focused on legislation of involuntary admissions and key actors involved in the admission procedure as well as most common reasons for involuntary admissions.
Results.
We analyzed the survey categorical data in themes, which highlight that both medical and legal actors are involved in involuntary admission procedures.
Conclusions.
We conclude that legal reasons for compulsory admission should be reworded in order to remove stigmatization of the patient, that raising awareness about involuntary admission procedures and patient rights with both patients and family advocacy groups is paramount, that communication about procedures should be widely available in lay-language for the general population, and that training sessions and guidance should be available for legal and medical practitioners. Finally, people working in the field need to be constantly aware about the ethical challenges surrounding compulsory admissions.
In a 2016 article published in this journal (Roig–Marín, 2016), I argued that the coinage of cyber-blends reflects our blended digital/physical relationships in today's world. The current pandemic has put a halt to our everyday lives and all forms of physical contact, and so technologies and digital experiences now play a more conspicuous role than ever. We have gone online and got used to vocabulary whose usage prior to COVID-19 was very limited (e.g. quarantine and pandemic) or known to very few (coronavirus, super-spreader, or the abbreviations PPE ‘personal protective equipment’ or WFH ‘working from home’), while coming to terms with the implications of others such as self-isolation, lockdown, or social distancing (which should be better called physical distancing as social closeness, albeit non-physically, is very much needed to get through these difficult times). Short pieces on coroneologisms have attested to the rise of many new lexical formations, mostly blends. According to Thorne (2020; also cited in CBC, 2020), more than 1,000 new words – both non-specialised and technical terminology – have been created during the current pandemic. Journalists and Twitter users are particularly prone to coin words displaying a high level of linguistic ingenuity; yet, the circulation of that lexis may be very limited. The present note overviews some of the most widely spread vocabulary related to our new COVID-19 reality, coming from the laity rather than from medical or scientific professionals. Alongside terms like social distancing and lockdown, less technical and more playful vocabulary has transcended linguistic boundaries. Particular attention will be paid to examples from European languages whose word-stocks share a common Latinate substratum, likewise central to scientific communication.
Aim of the study: to examine the role of endocannabinoids and CB1 receptors in psychosocial (PS) stress in mice. PS stress was induced in C57Bl/6 mice by resident-intruder paradigm (Brzózka et al. 2010). After 3 weeks PS stress anandamide (AEA), 2-arachidonoylglycerol (2-AG), N-oleoylethanolamine (OEA) and palmitoylethanolamide (PEA) were estimated in hippocampus, prefrontal cortex, striatum and cerebellum. Identically stressed and control mice (N = 15) were injected with WIN55212.2 (3 mg/kg) ± Rimonabant (3 mg/kg). Functional Observational Battery (FOB) (Golub et al., 2004), Open Field (OF), Prepulse Inhibition test (PPI) were studied. All behavioral recordings were done at night. Stressed mice showed significantly lowered AEA and OEA in Hippocampus, significant increase of 2-AG in Cortex, decrease of OEA in Striatum and increase of 2-AG in Cerebellum. Stressed mice displayed significantly lowered body weight gain, higher scratching activity, decrease of righting reflex time in FOB, higher distance travelled, time moving and hyperactivity in OF. In stressed mice WIN55212.2 significantly lowered rearings, increased righting reflex time, reduced distance travelled, time moving and hyperactivity in OF. Rimonabant did not significantly antagonize the effect of WIN55212.2 in stressed mice, but in controls. In controls WIN55212.2 significantly increased the number of scratches, reduced distance travelled, time moving and climbing and increased the startle response amplitude in PPI. The latter effect was significantly antagonized by Rimonabant. To sum up significant stress effects could be recorded in behavior, but less in PPI. PPI seems to be dependent on CB1-receptor processes but in case of stress endocannabinoids-activities may contribute.
to investigate the consequences of chronic psychosocial stress on behavior, endocannabinoids and CBR expression in prefrontal cortex (PFC) and striatum of mice.
Materials and Methods
Psychosocial stress was induced in adult C57Bl/6 mice by resident-intruder paradigm (Brzózka et al. 2011). After 3 weeks daily exposure to psychosocial stress for 1 hour, animals were studied during the rodent active phase (night) by behavioral tests such as Functional Observational Battery (FOB), Rota-Rod (R-R), Open Field (OF), Prepulse Inhibition test (PPI). After behavioral testing, mice were sacrificed. 4 mice brains (prefrontal cortex, dorsal striatum) were studied by LC-MS to estimate the concentration of anandamide (AEA), 2- arachidonoylglycerol (2-AG), N-oleoylethanolamine (OEA), palmitoylethanolamide (PEA) (coll. di Marzo). In Situ Hybridization (ISH)and Immunohistochemistry (IHCH) against CB1 receptor were performed on free floating brain coronal sections fixed by 4% paraformaldehyde (coll del Río).
Results
1. After psychosocial stress, mice displayed lower body weight (p<0.01), higher scratching and miccions activity compared to controls (p<0.05), decreased number of falls (p<0.01) and increased latency (p<0.05) in Rotarod. No effects in PPI were found. 2. In the same mice psychosocial stress reduced AEA levels in dorsal striatum and PFC (p<0.05). Endocannabinoids significantly showed an inverse relationship in PFC compared to striatum in control mice (AEA, p<0.001; 2-AG, p<0.001; OEA, p<0.001) and in psychosocially stressed mice (PEA, p<0.001; OEA, p<0.001). 3. Psychosocial stress increased the protein CBR1 expression in striatum (p<0.05) but not in prefrontal cortex.
Conclusion
Chronic psychosocial stress significantly changes behavior, endocannabinoids, CB receptor function and the striatal-cortical connectivity. These changes may contribute to vulnerability for psychosis and addiction.