The GINI project investigates the dynamics of inequality among populations over the long term by synthesising global archaeological housing data. This project brings archaeologists together from around the world to assess hypotheses concerning the causes and consequences of inequality that are of relevance to contemporary societies globally.

Most earthen burial mounds of eastern North America have been destroyed—or have they? We review geophysical methods for assessing whether leveled mounds retain intact deposits or features. Magnetic survey holds promise for locating and evaluating leveled mounds because it is rapid and sensitive to magnetic variations associated with anticipated features such as pits and deposits of mound fill. As a case study, we discuss our magnetic survey of the Gast Farm site (13LA12) in eastern Iowa. The survey covered 8.64 ha, encompassing loci of one previously reported mound and possible geometric earthworks as well as Middle and Late Woodland habitation areas. Interpretation of survey results incorporated quantitative differentiation of magnetic anomaly types using GIS techniques, along with standard visual inspection. We found no evidence of geometric earthworks but identified at least six leveled mounds. Displaced mound fill appears to account for the earthwork-like features. We conclude that leveled mounds are detectable and may retain subsurface integrity. Their associated features, including burials, may be identifiable even when above-ground evidence has disappeared.

Together, the concepts of heterarchy and collective action offer potential explanations for how early state societies may have established high degrees of civic coordination and sophisticated craft industries in the absence of exclusionary political strategies or dominant centralized political hierarchies. The Indus civilization (c. 2600–1900 bc) appears to have been heterarchical, which raises critical questions about how its infrastructure facilitated collective action. Digital re-visitation of early excavation reports provides a powerful means of re-examining the nuances of the resulting datasets and the old interpretations offered to explain them. In an early report on excavations at Mohenjo-daro, the Indus civilization's largest city, Ernest Mackay described a pair of small non-residential structures at a major street intersection as a ‘hostel’ and ‘office’ for the ‘city fathers’. In this article, Mackay's interpretation that these structures had a public orientation is tested using a geographical information systems approach (GIS) and 3D models derived from plans and descriptions in his report. In addition to supporting aspects of Mackay's interpretation, the resulting analysis indicates that Mohenjo-daro's architecture changed through time, increasingly favouring smaller houses and public structures. Close examination of these small public structures also suggests that they may at times have been part of a single complex.

Environmental perturbations during early mammalian development can affect aspects of offspring growth and cardiovascular health. We have demonstrated previously that maternal gestational dietary protein restriction in mice significantly elevated adult offspring systolic blood pressure. Therefore, the present study investigates the key mechanisms of blood pressure regulation in these mice. Following mating, female MF-1 mice were assigned to either a normal-protein diet (NPD; 18 % casein) or an isocaloric low-protein diet throughout gestation (LPD; 9 % casein), or fed the LPD exclusively during the pre-implantation period (3·5 d) before returning to the NPD for the remainder of gestation (Emb-LPD). All offspring received standard chow. At 22 weeks, isolated mesenteric arteries from LPD and Emb-LPD males displayed significantly attenuated vasodilatation to isoprenaline (P = 0·04 and P = 0·025, respectively), when compared with NPD arteries. At 28 weeks, stereological analysis of glomerular number in female left kidneys revealed no significant difference between the groups. Real-time RT-PCR analysis of type 1a angiotensin II receptor, Na+/K+ ATPase transporter subunits and glucocorticoid receptor expression in male and female left kidneys revealed no significant differences between the groups. LPD females displayed elevated serum angiotensin-converting enzyme (ACE) activity (P = 0·044), whilst Emb-LPD males had elevated lung ACE activity (P = 0·001), when compared with NPD offspring. These data demonstrate that elevated offspring systolic blood pressure following maternal gestational protein undernutrition is associated with impaired arterial vasodilatation in male offspring, elevated serum and lung ACE activity in female and male offspring, respectively, but kidney glomerular number in females and kidney gene expression in male and female offspring appear unaffected.