The chronology of human presence in Chontales, central Nicaragua, was established by Franck Gorin (1989), who applied a combination of absolute dates and ceramic associations to build a sequence comprised of six different phases. However, interpretative and chronological issues have arisen due to two main problems. First, the sequence was based on stylistic associations to polychrome ceramics from Pacific Nicaragua. Second, the sample analyzed by Gorin shows contextual bias and lacks sufficient absolute dates. As a result, a comprehensive regional research plan was established to test the current accepted chronology, and redefine it where necessary. In this paper, we present the first date list for the Valley of Juigalpa, Mayales River subbasin (Chontales, Nicaragua). Contrary to Gorin’s proposal, which established a chronology from 500 BC through AD 1600, reports on new seventeen radiocarbon (14C) assays show a cultural sequence from 1420 ± 30 BP, or cal AD 595–660 (±2σ) through the present.