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8 results

  • Biological Applications at the Cutting Edge of Cryo-Electron Microscopy

  • Rebecca S. Dillard, Cheri M. Hampton, Joshua D. Strauss, Zunlong Ke, Deanna Altomara, Ricardo C. Guerrero-Ferreira, Gabriella Kiss, Elizabeth R. Wright
  • Journal:
    / Volume 24 / Issue 4 / August 2018
    Published online by Cambridge University Press:
    03 September 2018, pp. 406-419
    Print publication:
    August 2018
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  • Cryo-electron microscopy (cryo-EM) is a powerful tool for macromolecular to near-atomic resolution structure determination in the biological sciences. The specimen is maintained in a near-native environment within a thin film of vitreous ice and imaged in a transmission electron microscope. The images can then be processed by a number of computational methods to produce three-dimensional information. Recent advances in sample preparation, imaging, and data processing have led to tremendous growth in the field of cryo-EM by providing higher resolution structures and the ability to investigate macromolecules within the context of the cell. Here, we review developments in sample preparation methods and substrates, detectors, phase plates, and cryo-correlative light and electron microscopy that have contributed to this expansion. We also have included specific biological applications.

  • Practical Strategies for cryo-CLEM Experiments

  • Cheri M. Hampton
  • Journal:
    / Volume 23 / Issue S1 / July 2017
    Published online by Cambridge University Press:
    04 August 2017, pp. 1400-1401
    Print publication:
    July 2017
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