A limited number of herbicides and sites of action are registered for use in sugarcane in Louisiana. Repeated use of the same sites of action can lead to the evolution of herbicide resistance in weeds. Therefore, it is critical to evaluate additional sites of action to provide growers with options for rotating herbicides to reduce the risk of resistance. Topramezone, indaziflam, and a formulation including mesotrione, bicyclopyrone, atrazine, and S-metolachlor, along with more common herbicide applications (pendimethalin, and metribuzin, clomazone, and diuron), were evaluated in the spring for injury to sugarcane, weed control, sugarcane yield, and sugar yield. Of these treatments, clomazone applied with diuron was the only herbicide combination to consistently injure the crop, with injury estimates ranging from 11 to 36%, which frequently resulted in reduced sugar yield with losses between 2.3% to 24.1% of the non-treated control. In most treatments, an increase in itchgrass counts was observed between harvests, indicating that additional control strategies will be needed in fields infested with this weed. However, topramezone alone and with triclopyr was well tolerated by sugarcane, with injuries ranging from 0 to 11% two weeks after treatment. Indaziflam and combined application of mesotrione, bicyclopyrone, atrazine, and S-metolachlor injury was at or under 10% two weeks after treatment. The tolerance of sugarcane for these herbicides suggests that they can be incorporated into weed management strategies in sugarcane. These herbicides would increase the sites of action available to be applied in sugarcane and help mitigate the risk of herbicide-resistant weeds.

Numerous annual and perennial weeds infest sugarcane. End-season weed infestations are managed before sugarcane is replanted by fallowing (cultivation and sequential glyphosate applications) or by rotating to glyphosate-tolerant soybean in Louisiana. With the occurrence of perennial grasses and glyphosate-resistant weeds, growers need to utilize alternative late POST (LPOST) herbicide programs in soybean to reduce weed infestations in newly planted sugarcane (soybean-sugarcane rotation). Current rotational restrictions limit the use of acifluorfen, clethodim, fomesafen, and quizalofop to control troublesome weeds before soybean harvest and the subsequent planting of sugarcane. However, there is a lack of information on the carryover effects of these soybean herbicides on newly planted sugarcane. Field experiments were conducted at Schriever, LA, and St. Gabriel, LA, in 2017 to 2018 and in 2020 to 2021 to determine sugarcane injury and yield component response to herbicides labeled for LPOST applications in soybean, including acifluorfen, clethodim, fomesafen, lactofen, and quizalofop, applied at the field-use rates (1X) 45 d prior to or immediately after sugarcane planting. Separate field experiments were conducted at those two locations in Louisiana in 2018 to 2019 and in 2020 to 2021 to determine sugarcane injury and yield component response to five rates of fomesafen applied immediately after sugarcane planting. Results of the herbicide screening experiment showed no reductions in sugarcane shoot and stalk population, stalk height, sugarcane yield, sucrose content, or sucrose yield from the selected herbicides at either application timing. Fomesafen applied at 790 (2X) and 1,580 (4X) g ha−1 resulted in 7% and 13% average visible injury to sugarcane at 27 d after treatment (DAT), respectively; injury symptoms persisted until 62 DAT. Transient injury observed at 62 DAT did not correspond to reduced sugarcane stalk population, height, sucrose content, sugarcane yield, or sucrose yield. This research indicates a potentially low risk of carryover and yield loss in newly planted sugarcane from late-season applications of selected soybean herbicides.

Controlling weeds is a critically important task in sugarcane production systems. Weeds compete for light, nutrients, and water, and if they are not managed properly can negatively impact sugarcane yields. Accurate detection of weeds versus desired plants was assessed using hyperspectral and pigment analyses. Leaf samples were collected from four commercial Louisiana sugarcane varieties, and nine weed species commonly found in sugarcane fields. Hyperspectral leaf reflectance data (350 to 850 nm) were collected from all samples. Plant pigment (chlorophylls and carotenoids) levels were also determined using high-performance liquid chromatography, and concentrations were determined using authentic standards and leaf area. In all cases, leaf reflectance data successfully differentiated sugarcane from weeds using canonical discrimination analysis. Linear discriminant analysis showed that the accuracy of the classification varied from 67% to 100% for individual sugarcane varieties and weed species. In all cases, sugarcane was not misclassified as a weed. Plant pigment levels exhibited marked differences between sugarcane varieties and weed species with differences in chlorophyll and carotenoid explaining much of the observed variation in reflectance. The ratio of chlorophyll a to chlorophyll b showed significant differences between sugarcane and all weed species. The successful implementation of this technology as either an airborne system to scout and map weeds or a tractor-based system to identify and spray weeds in real-time would offer sugarcane growers a valuable tool for managing their crops. By accurately targeting weeds in sugarcane fields that are emerged and growing, the total amount of herbicide applied could be decreased, resulting in cost savings for the grower and reduced environmental impacts.

Sugarcane infested with bermudagrass and harvested as seed cane introduces the potential for weedy propagules to reinfest fields. Research was conducted in 2018 and 2019 following sugarcane harvest for seed cane to evaluate bermudagrass management with photosystem II (PSII)- and 4-hydroxyphenylpyruvate dioxygenase (HPPD)–inhibiting herbicides applied alone or mixed with triclopyr. Combinations of diuron at 2.8 kg ha–1 with clomazone at 1.5 kg ha–1 or triclopyr at 1.1 kg ha–1 and hexazinone at 0.74 kg ha–1 with triclopyr applied early POST (EPOST) in mid-February injured bermudagrass 85% to 86%, and injury was greater than diuron or hexazinone alone (16% and 10%) in mid-March. Bermudagrass injury decreased to 45% to 56% for these combination treatments by April; however, injury differences were similar to the earlier rating. Late POST (LPOST) mid-March application of these treatments indicated similar bermudagrass injury trends when evaluated in early April. By mid-May, however, no treatment resulted in greater than 18% bermudagrass injury. Clomazone plus diuron applied LPOST resulted in 19% sugarcane injury by early April; however, all other treatments resulted in 7% sugarcane injury or less. In mid-May, a mid-April EPOST application of topramezone at 0.025 kg ha–1 plus triclopyr at 1.1 kg ha–1 resulted in 62% bermudagrass injury, which was equivalent to that observed with other topramezone rates in this combination (0.012 and 0.037 kg ha–1) (54% to 60%). Bermudagrass injury from triclopyr mixed with mesotrione (32%) or triclopyr mixed with atrazine, mesotrione, and S-metolachlor (47% to 55%) resulted in bermudagrass injury similar to that with topramezone plus triclopyr (54% to 62%). Data showed the flexibility of triclopyr when mixed with several HPPD- or PSII-inhibitor herbicides for bermudagrass management in a Louisiana sugarcane cropping system. Greater flexibility in application timing for HPPD-inhibitor herbicides than for PSII-inhibitor herbicides (diuron or hexazinone), and mixed with triclopyr, may suppress bermudagrass POST in April and May with minimal sugarcane injury.

Itchgrass [Rottboellia cochinchinensis (Lour.) W.D. Clayton] is among the most troublesome weeds in subtropical climates where sugarcane (Saccharum spp. interspecific hybrids) is cultivated. Two R. cochinchinensis biotypes commonly infest sugarcane in Louisiana. The Louisiana-1 biotype is daylength neutral, but Louisiana-2 flowered when daylength decreased to 13 h. Coupled with biotype diversity, seedling emergence has been reported to occur earlier in the growing season, as sugarcane emerged from winter dormancy. Both R. cochinchinensis biotypes were established in a common garden experiment in Louisiana during periods of sugarcane development and field preparation to simulate discontinuous emergence. Plant height and raceme production were recorded weekly for each biotype and establishment timing; aboveground biomass was harvested in autumn. Louisiana’s subtropical humid climate stimulated rapid plant growth that typically began in May and persisted through September. Without sugarcane competition, maximum R. cochinchinensis heights for Louisiana-1 and Louisiana-2 were 206 and 179 cm and growing degree days to 20-cm height in 2017 ranged from 546 to 832 and 865 to 1,160, respectively. Slower initial growth reported with Louisiana-2 would allow more time for growers to treat escaped plants with POST herbicides. Total raceme production, by autumn, was zero for Louisiana-2 established in June or later, but Louisiana-1 established in June produced up to 202 racemes. The present study demonstrated the importance of managing the Louisiana-2 biotype in March and April to limit seed production, but fields infested with Louisiana-1 were at greater risk for potential crop yield loss, because plants produced high quantities of seed when established over a wide period of time.

Burning postharvest sugarcane residue is a standard practice to remove extraneous leaf material before spring regrowth. Live-fires were simulated from field-collected postharvest sugarcane residue and seeds of divine nightshade and itchgrass were exposed to dry and moistened postharvest residue (PHR) at four densities (6.1, 12.1, 18.2, and 24.2 Mg ha−1) and a nonburned control. The moisture content of residue exposed to simulated rainfall was 14% more in Experiment 2 than Experiment 1; however, burning PHR with 44% moisture when wind speeds were lower allowed the fire to continue and created a smoldering effect that reduced weed emergence by 23% when compared with burning PHR with 30% moisture during breezy conditions. The moistened 6.1 Mg ha−1 PHR treatment resulted in 53% more divine nightshade and itchgrass emergence when compared with dry 6.1 Mg ha−1 PHR after burning, and greater emergence was attributed to more seed survival for divine nightshade than itchgrass. The PHR moisture condition failed to influence the burn duration; however, the burn duration increased 103% and 56% as the amount of PHR increased from 6.1 to 12.1 Mg ha−1 and 12.1 to 18.2 Mg ha−1, respectively. The combination of high wind speeds and moistened PHR did not enhance the maximum burn temperature near the soil surface, but surface-deposited divine nightshade and itchgrass seeds were susceptible to prolonged exposure times at 100 C. Burning PHR from fields with poor stands or older ratoon, especially when PHR is abundantly wet, will not produce temperatures lethal to divine nightshade and itchgrass seeds. The fluid-filled and fleshy content that comprises divine nightshade fruit protected seed from short durations of high temperatures, but may not insulate seeds long enough when exposed to a smoldering fire.

Knowledge of the effects of burial depth and burial duration on seed viability and, consequently, seedbank persistence of Palmer amaranth (Amaranthus palmeri S. Watson) and waterhemp [Amaranthus tuberculatus (Moq.) J. D. Sauer] ecotypes can be used for the development of efficient weed management programs. This is of particular interest, given the great fecundity of both species and, consequently, their high seedbank replenishment potential. Seeds of both species collected from five different locations across the United States were investigated in seven states (sites) with different soil and climatic conditions. Seeds were placed at two depths (0 and 15 cm) for 3 yr. Each year, seeds were retrieved, and seed damage (shrunken, malformed, or broken) plus losses (deteriorated and futile germination) and viability were evaluated. Greater seed damage plus loss averaged across seed origin, burial depth, and year was recorded for lots tested at Illinois (51.3% and 51.8%) followed by Tennessee (40.5% and 45.1%) and Missouri (39.2% and 42%) for A. palmeri and A. tuberculatus, respectively. The site differences for seed persistence were probably due to higher volumetric water content at these sites. Rates of seed demise were directly proportional to burial depth (α=0.001), whereas the percentage of viable seeds recovered after 36 mo on the soil surface ranged from 4.1% to 4.3% compared with 5% to 5.3% at the 15-cm depth for A. palmeri and A. tuberculatus, respectively. Seed viability loss was greater in the seeds placed on the soil surface compared with the buried seeds. The greatest influences on seed viability were burial conditions and time and site-specific soil conditions, more so than geographical location. Thus, management of these weed species should focus on reducing seed shattering, enhancing seed removal from the soil surface, or adjusting tillage systems.

Palmer amaranth (Amaranthus palmeri S. Watson) is a problematic weed encountered in U.S. cotton (Gossypium hirsutum L.) and soybean [Glycine max (L.) Merr.] production, with infestations spreading northward. This research investigated the influence of planting date (early, mid-, and late season) and population (AR, IN, MO, MS, NE, and TN) on A. palmeri growth and reproduction at two locations. All populations planted early or midseason at Throckmorton Purdue Agricultural Center (TPAC) and Arkansas Agriculture Research and Extension Center (AAREC) measured 196 and 141 cm or more, respectively. Amaranthus palmeri height did not exceed 168 and 134 cm when planted late season at TPAC and AAREC, respectively. Early season planted A. palmeri from NE grew to 50% of maximum height 8 to 13 d earlier than all other populations under TPAC conditions. In addition, the NE population planted early, mid-, and late season achieved 50% inflorescence emergence 5, 4, and 6 d earlier than all other populations, respectively. All populations established at TPAC produced fewer than 100,000 seeds plant−1. No population planted at TPAC and AAREC produced more than 740 and 1,520 g plant−1 of biomass at 17 and 19 wk after planting, respectively. Planting date influenced the distribution of male and female plants at TPAC, but not at AAREC. Amaranthus palmeri from IN and MS planted late season had male-to-female plant ratios of 1.3:1 and 1.7:1, respectively. Amaranthus palmeri introduced to TPAC from NE can produce up to 7,500 seeds plant−1 if emergence occurs in mid-July. An NE A. palmeri population exhibited biological characteristics allowing it to be highly competitive if introduced to TPAC due to a similar latitudinal range, but was least competitive when introduced to AAREC. Although A. palmeri originating from different locations can vary biologically, plants exhibited environmental plasticity and could complete their life cycle and contribute to spreading populations.

Adoption of soybean that is resistant to 2,4-D will result in more use of glyphosate plus 2,4-D premixes and tank mixtures. Preliminary whole-plant greenhouse assays confirm most Palmer amaranth populations found in Indiana are glyphosate-resistant (GR), and some biotypes exhibit tolerance to 2,4-D amine. Dose–response experiments were conducted to determine the level of glyphosate resistance and 2,4-D amine tolerance in four Palmer amaranth biotypes. A premix formulation of glyphosate plus 2,4-D choline was also evaluated. The R1, R2, and R3 biotypes were 31- to 66-fold more resistant to glyphosate (R:S ratio) than the S1 biotype based on the herbicide dose to cause 90% mortality (LD90). The maximum POST rate of the premix formulation of Enlist Duo® labeled in ‘Enlist®’ soybean is 2,195 g ae ha−1. When separated by active ingredient, the maximum POST rate of Enlist Duo® is equivalent to 1,141 and 1,054 g ae ha−1 of glyphosate and 2,4-D choline, respectively. In the absence of glyphosate, the maximum rate of 2,4-D (1,054 g ae ha−1) in the premix formulation of Enlist Duo® controlled S1, R2, and R3 biotypes, but failed to control all plants from the R1 biotype. Estimates for LD90 showed the R1 biotype was 3-fold more tolerant than the S1 biotype to 2,4-D amine. However, no plants survived the 1,155 g ae ha−1 (600 g ae ha−1 of glyphosate plus 555 g ae ha−1 2,4-D) treatment with the premix formulation of glyphosate plus 2,4-D choline. Overall, results from this experiment suggest GR Palmer amaranth that also exhibits increased tolerance to 2,4-D amine will be difficult to control with glyphosate or 2,4-D alone, but can be controlled POST with Enlist Duo® at lower than labeled field rates (1,618 to 2,195 g ae ha−1).

A field study was conducted for the 2014 and 2015 growing season in Arkansas, Indiana, Illinois, Missouri, Ohio, and Tennessee to determine the effect of cereal rye and either oats, radish, or annual ryegrass on the control of Amaranthus spp. when integrated with comprehensive herbicide programs in glyphosate-resistant and glufosinate-resistant soybean. Amaranthus species included redroot pigweed, waterhemp, and Palmer amaranth. The two herbicide programs included were: a PRE residual herbicide followed by POST application of foliar and residual herbicide (PRE/POST); or PRE residual herbicide followed by POST application of foliar and residual herbicide, followed by another POST application of residual herbicide (PRE/POST/POST). Control was not affected by type of soybean resistance trait. At the end of the season, herbicides controlled 100 and 96% of the redroot pigweed and Palmer amaranth, respectively, versus 49 and 29% in the absence of herbicides, averaged over sites and other factors. The PRE/POST and PRE/POST/POST herbicide treatments controlled 83 and 90% of waterhemp at the end of the season, respectively, versus 14% without herbicide. Cover crop treatments affected control of waterhemp and Palmer amaranth and soybean yield, only in the absence of herbicides. The rye cover crop consistently reduced Amaranthus spp. density in the absence of herbicides compared to no cover treatment.

Herbicide-resistant Amaranthus spp. continue to cause management difficulties in soybean. New soybean technologies under development, including resistance to various combinations of glyphosate, glufosinate, dicamba, 2,4-D, isoxaflutole, and mesotrione, will make possible the use of additional herbicide sites of action in soybean than is currently available. When this research was conducted, these soybean traits were still regulated and testing herbicide programs with the appropriate soybean genetics in a single experiment was not feasible. Therefore, the effectiveness of various herbicide programs (PRE herbicides followed by POST herbicides) was evaluated in bare-ground experiments on glyphosate-resistant Palmer amaranth and glyphosate-resistant waterhemp (both tall and common) at locations in Arkansas, Illinois, Indiana, Missouri, Nebraska, and Tennessee. Twenty-five herbicide programs were evaluated; 5 of which were PRE herbicides only, 10 were PRE herbicides followed by POST herbicides 3 to 4 wks after (WA) the PRE application (EPOST), and 10 were PRE herbicides followed by POST herbicides 6 to 7 WA the PRE application (LPOST). Programs with EPOST herbicides provided 94% or greater control of Palmer amaranth and waterhemp at 3 to 4 WA the EPOST. Overall, programs with LPOST herbicides resulted in a period of weed emergence in which weeds would typically compete with a crop. Weeds were not completely controlled with the LPOST herbicides because weed sizes were larger (≥ 15 cm) compared with their sizes at the EPOST application (≤ 7 cm). Most programs with LPOST herbicides provided 80 to 95% control at 3 to 4 WA applied LPOST. Based on an orthogonal contrast, using a synthetic-auxin herbicide LPOST improves control of Palmer amaranth and waterhemp over programs not containing a synthetic-auxin LPOST. These results show herbicides that can be used in soybean and that contain auxinic- or HPPD-resistant traits will provide growers with an opportunity for better control of glyphosate-resistant Palmer amaranth and waterhemp over a wide range of geographies and environments.

A total of four field experiments were conducted over a 2-yr period (2011 and 2012) near Mokane and Moberly, Missouri, to determine the control of glyphosate-resistant (GR) waterhemp with dicamba and glyphosate applied alone or as a tank-mix combination. In one experiment, dicamba was applied at 0.14, 0.28, 0.42, and 0.56 kg ae ha−1 with or without 0.86 kg ae ha−1 glyphosate to GR waterhemp plants 7.5, 15, and 30 cm in height. In a second experiment, sequential treatments of dicamba or dicamba plus glyphosate were applied 4, 7, and 14 d after the initial herbicide treatment to plants measuring either 7.5 or 23 cm in height. Control of GR waterhemp ranged from 7 to 62%, 11 to 40%, and 8 to 30% when applied to 7.5-, 15-, and 30-cm plants, respectively. Control of 7.5-cm GR waterhemp increased by 16 to 36%, and biomass reduction increased by 29 to 52% in response to 0.14, 0.28, 0.42, and 0.56 kg ha−1 dicamba plus glyphosate compared to these same rates of dicamba alone. When sequential dicamba-containing treatments were averaged across all treatments and application timings, GR waterhemp control ranged from 46 to 47%, and biomass reduction ranged from 55 to 66%. No differences in control were observed based on the timing of the sequential herbicide treatment. However, in terms of GR waterhemp biomass reduction, sequential treatments applied 4 d after the initial treatment reduced GR waterhemp biomass more than sequential treatments applied 14 d after the initial treatment. Results from these experiments indicate that, in the absence of crop competition, a single treatment of dicamba up to 0.56 kg ha−1 provides less than 62% control of GR waterhemp, and sequential dicamba plus glyphosate treatments targeting 7.5 cm plants are required to achieve at least 72% control.

Field experiments were conducted across two locations during 2011 and 2012 to evaluate herbicide options for the control of glyphosate-resistant (GR) giant ragweed and GR waterhemp in dicamba-resistant (DR) soybean. All herbicide treatments provided 91 to 100% control of GR giant ragweed 3 wk after treatment (WAT). Flumioxazin plus dicamba plus glyphosate applied preplant provided greater control and density reduction of GR giant ragweed than flumioxazin plus 2,4-D plus glyphosate. When flumioxazin plus dicamba plus glyphosate were applied preplant, the addition of dicamba to glyphosate at either the early-postemergence (EPOST) or mid-postemergence (MPOST) timing provided greater control and density reduction of GR giant ragweed than glyphosate alone. Regardless of the preplant treatment, delay of EPOST dicamba to the MPOST timing did not influence GR giant ragweed control or density reduction. In the GR waterhemp experiment, dicamba plus glyphosate applied sequentially provided 88 to 89% control and 90% density reduction at the EPOST and MPOST timings compared to only 24% control and 42% density reduction in response to glyphosate applied sequentially. Control and GR waterhemp density reduction did not improve with the addition of acetochlor to either the EPOST or late-postemergence (LPOST) timings. Flumioxazin plus chlorimuron applied PRE followed by dicamba plus glyphosate or dicamba plus glyphosate plus acetochlor provided greater control of GR waterhemp than glyphosate plus fomesafen or glyphosate alone applied EPOST. Results from this research indicate that dicamba applied once or sequentially and when timed appropriately to match the biology of the weed species can be utilized as a component of an integrated program for the management of GR weeds like giant ragweed and waterhemp in DR soybean.

Palmer amaranth and waterhemp have become increasingly troublesome weeds throughout the United States. Both species are highly adaptable and emerge continuously throughout the summer months, presenting the need for a residual PRE application in soybean. To improve season-long control of Amaranthus spp., 19 PRE treatments were evaluated on glyphosate-resistant Palmer amaranth in 2013 and 2014 at locations in Arkansas, Indiana, Nebraska, Illinois, and Tennessee; and on glyphosate-resistant waterhemp at locations in Illinois, Missouri, and Nebraska. The two Amaranthus species were analyzed separately; data for each species were pooled across site-years, and site-year was included as a random variable in the analyses. The dissipation of weed control throughout the course of the experiments was compared among treatments with the use of regression analysis where percent weed control was described as a function of time (the number of weeks after treatment [WAT]). At the mean (i.e., average) WAT (4.3 and 3.2 WAT for Palmer amaranth and waterhemp, respectively) isoxaflutole + S-metolachlor + metribuzin had the highest predicted control of Palmer amaranth (98%) and waterhemp (99%). Isoxaflutole + S-metolachlor + metribuzin, S-metolachlor + mesotrione, and flumioxazin + pyroxasulfone had a predicted control ≥ 97% and similar model parameter estimates, indicating control declined at similar rates for these treatments. Dicamba and 2,4-D provided some, short-lived residual control of Amaranthus spp. When dicamba was added to metribuzin or S-metolachlor, control increased compared to dicamba alone. Flumioxazin + pyroxasulfone, a currently labeled PRE, performed similarly to treatments containing isoxaflutole or mesotrione. Additional sites of action will provide soybean growers more opportunities to control these weeds and reduce the potential for herbicide resistance.

Carrier water pH, hardness, coapplied foliar fertilizer, water conditioning agents, and plant height are critical considerations for optimum herbicide performance. Field studies were conducted to evaluate the effect of carrier water pH (4, 6.5, and 9) and zinc (Zn) or manganese (Mn) foliar fertilizer on mesotrione for horseweed and Palmer amaranth control. Additionally, effect of carrier water pH and foliar fertilizer was evaluated on 7.5-, 12.5-, and 17.5-cm tall horseweed. Greenhouse treatments consisted of carrier water pH and foliar fertilizer (Zn, Mn, or without fertilizer); or water hardness (0 to 1,000 mg L−1) in the presence or absence of ammonium sulfate (AMS) for mesotrione control of giant ragweed, horseweed, and Palmer amaranth. Mesotrione activity was greater on horseweed with carrier water pH 6.5 compared to pH 4 or 9. Coapplied Zn fertilizer reduced mesotrione activity on Palmer amaranth in the field study in 2014 and horseweed in the greenhouse study. Mesotrione efficacy was greatly influenced by horseweed height. Percent control ranged from 96 to 99%, 75 to 89%, or 61 to 64% with mesotrione applied on 7.5-, 12.5-, or 17.5-cm tall horseweed, respectively, and results were similar for plant height and dry weight reduction. Increasing carrier water hardness from 0 to 1,000 mg L−1 reduced mesotrione efficacy 28, 18, and 18% (or greater) on giant ragweed, horseweed, and Palmer amaranth, respectively. The addition of AMS enhanced mesotrione efficacy 9, 6, or 9% (or greater) for giant ragweed, horseweed, and Palmer amaranth control, respectively. Mesotrione should be applied at near neutral carrier water pH, hardness < 200 mg L−1, and with AMS for achieving optimum weed control.