MicroRNAs (miRNAs) are endogenous, non-coding RNAs, which are functional in a variety of biological processes through post-transcriptional regulation of gene expression. However, the role of miRNAs in the interaction between Bacillus thuringiensis and insects remains unclear. In this study, small RNA libraries were constructed for B. thuringiensis-infected (Bt) and uninfected (CK) Spodoptera exigua larvae (treated with double-distilled water) using Illumina sequencing. Utilising the miRDeep2 and Randfold, a total of 233 known and 726 novel miRNAs were identified, among which 16 up-regulated and 34 down-regulated differentially expressed (DE) miRNAs were identified compared to the CK. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that potential target genes of DE miRNAs were associated with ABC transporters, fatty acid metabolism and MAPK signalling pathway which are related to the development, reproduction and immunity. Moreover, two miRNA core genes, SeDicer1 and SeAgo1 were identified. The phylogenetic tree showed that lepidopteran Dicer1 clustered into one branch, with SeDicer1 in the position closest to Spodoptera litura Dicer1. A similar phylogenetic relationship was observed in the Ago1 protein. Expression of SeDicer1 increased at 72 h post infection (hpi) with B. thuringiensis; however, expression of SeDicer1 and SeAgo1 decreased at 96 hpi. The RNAi results showed that the knockdown of SeDicer1 directly caused the down-regulation of miRNAs and promoted the mortality of S. exigua infected by B. thuringiensis GS57. In conclusion, our study is crucial to understand the relationship between miRNAs and various biological processes caused by B. thuringiensis infection, and develop an integrated pest management strategy for S. exigua via miRNAs.

In order to determine whether Taenia solium can be maintained in the laboratory using rodents as definitive hosts, six nude rats, 20 immunosuppressed Mongolian gerbils and 20 immunosuppressed Syrian hamsters were each inoculated through a stomach tube with three cysticerci recovered from SCID mice. No adult worms of T. solium were found in the intestinal tract of any of these 46 rodents. In addition, five immunosuppressed Syrian hamsters were fed with the same number of cysticerci enclosed in rodent muscles from SCID mice. Two of these hamsters were found to be infected 40 days post-infection, each harbouring a sexually developed worm in the intestinal tract. Although no eggs were produced, prepatent infections may be possible if a longer time was allowed for worm development. Moreover, the maintenance of the life cycle of T. solium in the laboratory using the rodent model can be established.

Normal and immunosuppressed mice were infected with oncospheres of Taenia saginata asiatica and T. solium. Although normal ICR mice were not susceptible to these two parasites, cysticerci were recovered from the immunosuppressed ones following venous injection. For T. s. asiatica, immunosuppressed ICR mice had an infection rate of 12.5% and six cysticerci of this parasite were recovered from three males. After injection of T. solium oncospheres, a high infection rate of 57% was obtained and 23 cysticerci were collected from 13 male immunosuppressed ICR mice. The immunosuppressed C57 mice had the highest infection rate (100%) and cysticercus recovery rate (2.4%) for T. solium. The infection rate and cysticercus recovery rate in six normal C57 mice were 40% and 3% respectively. The immunosuppressed ICR, Balb/c and C3H mice were also susceptible to T. s. asiatica.

To optimize flapping foil performance, in the current study we apply deep reinforcement learning (DRL) to plan foil non-parametric motion, as the traditional control techniques and simplified motions cannot fully model nonlinear, unsteady and high-dimensional foil–vortex interactions. Therefore, a DRL training framework is proposed based on the proximal policy optimization algorithm and the transformer architecture, where the policy is initialized from the sinusoidal expert display. We first demonstrate the effectiveness of the proposed DRL-training framework, learning the coherent foil flapping motion to generate thrust. Furthermore, by adjusting reward functions and action thresholds, DRL-optimized foil trajectories can gain significant enhancement in both thrust and efficiency compared with the sinusoidal motion. Last, through visualization of wake morphology and instantaneous pressure distributions, it is found that DRL-optimized foil can adaptively adjust the phases between motion and shedding vortices to improve hydrodynamic performance. Our results give a hint of how to solve complex fluid manipulation problems using the DRL method.

The role of Mn oxide in the abiotic formation of humic substances has been well demonstrated. However, information on the effect of crystal structure and surface-chemical characteristics of Mn oxide on this process is limited. In the present study, hexagonal and triclinic birnessites, synthesized in acidic and alkali media, were used to study the influence of the crystal-structure properties of birnessites on the oxidative polymerization of hydroquinone and to elucidate the catalytic mechanism of birnessites in the abiotic formation of humic-like polymers in hydroquinone-birnessite systems. The intermediate and final products formed in solution and solid-residue phases were identified by UV/Visible spectroscopy, atomic absorption spectrometry, Fourier-transform infrared spectroscopy, X-ray diffraction, solid-phase microextraction-gaschromatography-mas ss pectrometry, ion chromatography, and ultrafiltration. The degree of oxidative polymerization of hydroquinone wasenhanced with increase in the interlayer hydrated H+, the average oxidation state (AOS), and the specific surface area of birnessites. The nature of the functional groups of the humic-like polymers formed was, however, almost identical when hydroquinone was catalyzed by hexagonal and triclinic birnessites with similar AOS of Mn. The results indicated that crystal structure and surface-chemistry characteristics have significant influence on the oxidative activity of birnessites and the degree of polymerization of hydroquinone, but have little effect on the abiotic formation mechanism of humic-like polymers. The proposed oxidative polymerization pathway for hydroquinone isthat, asit approachesthe birnessite, it formsp recursor surface complexes. Asa strong oxidant, birnessite accepts an electron from hydroquinone, which is oxidized to 1,4-benzoquinone. The coupling, cleavage, polymerization, and decarboxylation reactionsaccompany the generation of 1,4-benzoquinone, lead to the release of CO2 and carboxylic acid fragments, the generation of rhodochrosite, and the ultimate formation of humic-like polymers. These findings are of fundamental significance in understanding the catalytic role of birnessite and the mechanism for the abiotic formation of humic substances in nature.

Lean hydrogen/air premixed flame flashback in a turbulent boundary layer over a flat plate is investigated using three-dimensional direct numerical simulation with detailed chemical kinetics. The upstream propagation of the flame takes place in near-wall turbulence and the interaction between the flame and the approaching reactant flow is studied. It is found that backflow regions are always present immediately upstream of flame bulges that are convex towards the reactants, confirming earlier observations. A flashback speed, including the effects of flame displacement speed and flow velocity, is introduced to quantify the flame flashback behaviour. This analysis indicates that the flashback speed is overall positive and it is considerably affected by the presence of the backflow regions. A budget analysis of the pressure transport equation is performed to explain the presence of the backflow regions. It is suggested that the positive dilatation and thermal diffusion terms near the leading edge of flame bulges are the main reasons for the pressure increase, leading to an adverse pressure gradient. The effects of the flame-induced adverse pressure gradient on the structures of the turbulent boundary layer are also investigated. It is revealed that the near-wall mean velocity and skin-friction coefficient are reduced due to the adverse pressure gradient. The coherent vortical structures of the boundary layer turbulence are lifted by the adverse pressure gradient. The analysis of the Reynolds stress component showed that the ejection event is augmented by combustion while the sweep event is attenuated, which facilitates the occurrence of flame flashback.

Purple nutsedge (Cyperus rotundus L.) is a globally distributed noxious weed that poses a significant challenge for control due to its fast and efficient propagation through the tuber, which is the primary reproductive organ. Gibberellic acid (GA3) has proven to be crucial for tuberization in tuberous plants. Therefore, understanding the relationship between GA3 and tuber development and propagation of C. rotundus will provide valuable information for controlling this weed. This study shows that the GA3 content decreases with tuber development, which corresponds to lower expression of bioactive GA3 synthesis genes (CrGA20ox, two CrGA3ox genes) and two upregulated GA3 catabolism genes (CrGA2ox genes), indicating that GA3 is involved in tuber development. Simultaneously, the expression of two CrDELLA genes and CrGID1 declines with tuber growth and decreased GA3, and yeast two-hybrid assays confirm that the GA3 signaling is DELLA-dependent. Furthermore, exogenous application of GA3 markedly reduces the number and the width of tubers and represses the growth of the tuber chain, further confirming the negative impact that GA3 has on tuber development and propagation. Taken together, these results demonstrate that GA3 is involved in tuber development and regulated by the DELLA-dependent pathway in C. rotundus and plays a negative role in tuber development and propagation.

Ankle rehabilitation robots are widely used due to nerve injuries and sports injuries leading to decreased mobility of the ankle joint. However, the motion of most ankle rehabilitation robots has distinctions with human ankle physiological structure. In order to achieve more accurate rehabilitation training of the ankle joint, this paper proposes a novel 3-UPU parallel rehabilitation mechanism. In a certain range, the mechanism can perform rotation around any axis within the midplane, which means that the mechanism can achieve non-fixed-point rotation around the instantaneous axis of the ankle joint. The mechanism has three degrees of freedom and can perform ankle pronation/supination and inversion/eversion movements. Taking into account the structural differences of different human bodies, the rotating axis of the mechanism can be adjusted in both height and angle. Then, the workspace of the mechanism was solved, and the size parameters of the mechanism are analyzed based on the characteristics of the size parameters of the mechanism and the motion range of the ankle. A genetic algorithm was employed to optimize the mechanism’s parameters. Next, the motion trajectory of the mechanism was planned, and the length change of the mechanism driving pair during the motion planning of the angle was obtained through kinematics simulation. Finally, experimental verification of the above rehabilitation training methods indicates that the mechanism meets the requirements of ankle rehabilitation.

This study is grounded upon the cognitive process and knowledge representation, analyzes the differences in perceptions between designers and users by applying schema theory. Per design process, we disassembled the semantic words that represent the design concept, and re-construct the representative visual imagery library. We experimented the imagery library with selective designers and users, and through their selections of the images, we uncovered: 1. The differences largely exist in concept interpretation and imagery selection between designers and users, which has strong relationship with their different schema; 2. The experiment revealed the fact that designers are inclined to understand the concept by disassembling the elements, and have obvious tendency of professionalism, while users’ interpretation prefers complete forms and life-oriented; 3. As compared with users, designers relatively prefer brand-new, creative elements in selecting visualized representation.

Prostrate water primrose is a troublesome weed in rice paddy fields. A study was conducted to determine the influence of environmental and agronomic factors on its emergence. The efficacy of herbicides on this species was also examined. The germination percentage of mature seeds remained above 90% within 180 d after harvest, indicating a low primary dormancy of this species. Light stimulated seed germination. Seeds buried deeper than 0.5 cm did not form seedlings. These results suggest that stale seedbed practices and deep tillage operations can mitigate the occurrence of this species in paddy fields. The optimum temperature for germination varied from 25/15 C to 35/25 C. The osmotic potential and salt concentration needed to inhibit 50% of maximum germination were −0.4 MPa and 197 mM, respectively. Seeds were tolerant to flooding and did not germinate at pH 8 to 10. The preemergence herbicides oxadiazon, oxadiargyl, and butachlor had excellent control efficacy on prostrate water primrose, with a 95.4% to 100% reduction in seedling number and a 99.2% to 100% reduction in biomass, respectively. The postemergence herbicides MCPA-Na + bentazone, bentazone, MCPA-Na, and fluroxypyr applied at the 2- to 3-leaf stage of prostrate water primrose provided a 90.6% to 100% reduction in seedling number and a 99.3% to 100% reduction in biomass. The results of this study can help in developing sustainable and effective integrated weed management strategies for controlling prostrate water primrose in paddy fields.

In the Late Neoarchaean, the lithosphere of the North China Craton (NCC) experienced a strong extensional event, which is of great significance for understanding the evolution of the continental crust in the Precambrian. In this study, a suite of mafic dykes from Shandong province in the northeastern NCC were investigated to determine the nature, timing and source of rift-related magma activities using zircon U–Pb data, whole-rock geochemistry and Nd–Hf isotopes. Zircon U–Pb dating of four dolerites by laser ablation – inductively coupled plasma – mass spectrometry (LA-ICP-MS) yielded weighted mean 207Pb/206Pb ages in the range 2509 ± 6.1 to 2537 ± 6.2 Ma (2σ, 95 % confidence interval). The mafic dykes are classified as alkaline rocks based on their K2O + Na2O contents (6.78–7.21 wt %) and belong to the shoshonitic series according to their K2O contents (3.23–3.36 wt %). The dolerites show low concentrations of light rare earth elements ((La/Yb)N between 7.17 and 8.55), positive Eu anomalies (Eu/Eu* between 1.12 and 1.27), positive Ba, K, Pb, Sr, Eu, Dy and Lu anomalies, and depleted U, Nb, Pr, Ta, P, Nd and Ti anomalies. The dykes are characterized by low initial (87Sr/86Sr)i (∼0.6969), positive εNd(t) values (0.2–0.8) and εHf(t) values (0.5–8.6) and relatively old mean Nd and Hf model age (2.73 Ga). Collectively, the data suggest that the mafic dykes were derived from the partial melting (10–20 %) of an isotope-depleted garnet–lherzolite mantle source that was hybridized through interaction with subducted lower crustal material. The parental magmas of these dykes underwent a certain number of crustal contaminations during magma ascent. The mafic magmatism represented in the form of the dyke swarms is considered to be a response to widespread lithospheric extension which affected the NCC at c. 2.5 Ga during the Neoarchaean.

Patients with cirrhosis experience worse health-related quality of life (HRQoL), and attempts are warranted further exploration of modifiable factors to improve HRQoL. Data on the impact of malnutrition risk on HRQoL among cirrhosis are limited; thus, we aimed to strengthen understanding by clarifying the relationship between nutritional status and low HRQoL in patients with decompensated cirrhosis. Consecutive inpatients with cirrhosis attending our department within a tertiary hospital were studied. Generic health profiles and malnutrition risk were evaluated by the EuroQol-5D (EQ-5D) and Royal Free Hospital-Nutritional Prioritizing Tool (RFH-NPT) score, respectively. Multiple linear regression analysis was used to determine association of malnutrition risk with low HRQoL. In this cohort of 364 patients with median age of 64 years and 49·5 % male, 55·5 % of the study population reported impairment pertinent to HRQoL in at least one dimension in terms of the EQ-5D. Moreover, malnutrition risk (RFH-NPT score: β coefficient = −0·114, P = 0·038) was proved to be independently associated with poor HRQoL in multiple analysis, after adjustment for significant variables like age, BMI and markers of decompensation. Notably, we found that health dimensions representing physical function (i.e. mobility, self-care and usual activities) are substantially affected, while malnourished patients reported less frequencies of complaints in other domain such as anxiety/depression. In conclusion, the risk of malnutrition assessed by the RFH-NPT score is independently associated with low HRQoL. It is operational to improve HRQoL by identifying patients at high malnutrition risk and providing timely nutrition treatment.

In the present study, turbulent particle-laden boundary layer combustion over a flat plate is investigated using direct numerical simulation (DNS). A two-way coupled Eulerian–Lagrangian point particle method is used for the solid phase. The effects of particle Stokes numbers, mass loadings and chemical reactions on the interactions between particles and boundary layer turbulence in the near-wall region are explored. It was found that particle heat transfer is dominant over wall heat transfer in the reacting case with heavy particles and large mass loadings, resulting in a lower fluid temperature. Particle accumulation due to the turbophoresis effect in the near-wall region is observed, which is more prominent in the cases with a large Stokes number. The turbophoresis effect is examined via the magnitude of streamwise vorticity $\varOmega _x$. It is shown that $\varOmega _x$ is attenuated by heavy particles, and the attenuation increases with increasing mass loadings. Therefore, particle wall–accumulation is less prominent in the cases with large mass loadings. Compared with the non-reacting cases, the distribution of particles is more inhomogeneous for the reacting cases, where the particles move faster due to intense reactions with increasing wall-normal distance. Finally, the flow topologies and the Reynolds stress anisotropy are examined to understand turbulence modulation by combustion and particles. It was suggested that light particles augment the vortex-dominant topologies, whereas heavy particles have an opposite effect. The anisotropy mapping of the Reynolds stress shows that the flows become more one-dimensional in the near-wall region for the cases with combustion and/or large mass loadings.