Species of epiphytic microbiota are closely associated with the fermentation performance of natural forage silage. This study aimed to evaluate the dynamic microbial communities, fermentation parameters, and aerobic stability of Napier grass silage from the same variety and growth period but harvested from three different regions (NGP1, NGP2, and NGP3). After 60 days of ensiling, triplicate silos were opened for sampling and testing aerobic stability. The epiphytic microbiota with higher relative abundances in fresh Napier grass (NGP1, NGP2, and NGP3) were Weissella, Enterobacter, and Lactococcus, respectively. After 60 days of ensiling, NGP3 exhibited higher fermentation quality, indicated by higher lactic acid (LA) concentration and lower pH than that of NGP1 and NGP2. The NH3–N content of all treatments was lower than 100 g/kg total nitrogen. Compared with NGP1 and NGP2 silage, NGP3 silage exhibited a sharp rise in pH and LA consumption during air exposure. After 7 days of air exposure, NGP3 had higher ethanol concentrations and pH. Ruminiclostridium_5, Pediococcus, and Lactobacillus predominated in NGP1, NGP2, and NGP3 silages, respectively, whereas Candida and Monascus predominated in air-exposed NGP3 silage. The bacterial co-occurrence networks from fresh samples to ensiling and air exposure became more complex; however, NGP3 had a higher negative correlation with co-occurrence after air exposure. Different regions had significant effects on the fermentation patterns, bacterial communities, and aerobic stability of Napier grass silage. This was mainly due to variable epiphytic microbiota. Higher fermentation quality of Napier grass silage may also result in accelerated spoilage due to air exposure. Candida and Monascus were primarily responsible for the lower dry matter recovery and higher ethanol contents and air exposure spoilage of Napier grass silage.

The dynamics of compound drops impacting on a flat substrate is numerically investigated using a ternary-fluid diffuse-interface method, with the aim of assessing the effect of a density difference between the inner and outer droplets (denoted by $\lambda$) on the evolution of the interfaces. With the help of numerical simulations, we find that, at the intermediate stage of drop impact, the inner droplet exhibits a self-similar deformation at $\lambda =1$ and relatively high Weber number, and experiences more or less a uniform acceleration for various $\lambda$. In particular, the acceleration magnitude at $\lambda \ne 1$ can be correlated with the acceleration at $\lambda =1$ and the Atwood number. When the inner droplet is denser than the outer one, a lamella occurs at the spreading front of the inner droplet. We present a scaling analysis of the thickness of the lamella, and the resultant theoretical prediction is in good agreement with numerical results. At the maximal spreading of the compound drop, a bulging structure is formed around the symmetry axis due to the presence of the inner droplet, thereby effectively reducing the liquid supply to the spreading front and leading to a decrease of maximal spreading ratio $\beta _{max}$ as compared with a pure drop. We proposed a corrected Weber number $We^*_\lambda$ by taking account of the combined effects of $\lambda$, volume fraction of the inner droplet, Weber number and morphology of the compound drop. Integrating $We^*_\lambda$ with the universal model of $\beta _{max}$ for impacting pure drops, we successfully build up a new model for predicting the maximal spreading ratio of impacting compound drops with various $\lambda$.

N-acetylcysteine (NAC) possesses a strong capability to ameliorate high-fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) in mice, but the underlying mechanism is still unknown. Our study aimed to clarify the involvement of long non-coding RNA (lncRNA) in the beneficial effects of NAC on HFD-induced NAFLD. C57BL/6J mice were fed a normal-fat diet (10 % fat), a HFD (45 % fat) or a HFD plus NAC (2 g/l). After 14-week of intervention, NAC rescued the deleterious alterations induced by HFD, including the changes in body and liver weights, hepatic TAG, plasma alanine aminotransferase, plasma aspartate transaminase and liver histomorphology (haematoxylin and eosin and Oil red O staining). Through whole-transcriptome sequencing, 52 167 (50 758 known and 1409 novel) hepatic lncRNA were detected. Our cross-comparison data revealed the expression of 175 lncRNA was changed by HFD but reversed by NAC. Five of those lncRNA, lncRNA-NONMMUT148902·1 (NO_902·1), lncRNA-XR_001781798·1 (XR_798·1), lncRNA-NONMMUT141720·1 (NO_720·1), lncRNA-XR_869907·1 (XR_907·1), and lncRNA-ENSMUST00000132181 (EN_181), were selected based on an absolute log2 fold change value of greater than 4, P-value < 0·01 and P-adjusted value < 0·01. Further qRT-PCR analysis showed the levels of lncRNA-NO_902·1, lncRNA-XR_798·1, and lncRNA-EN_181 were decreased by HFD but restored by NAC, consistent with the RNA sequencing. Finally, we constructed a ceRNA network containing lncRNA-EN_181, 3 miRNA, and 13 mRNA, which was associated with the NAC-ameliorated NAFLD. Overall, lncRNA-EN_181 might be a potential target in NAC-ameliorated NAFLD. This finding enhanced our understanding of the biological mechanisms underlying the beneficial role of NAC.

This paper exploited an alternative approach to prepare high-quality speckle patterns by uniformly dispersing nano-silica particles onto sample surfaces, helping digital image correlation (DIC) acquire the maximum spatial resolution of local strain up to 92 nm. A case study was carried out by combining this speckle pattern fabrication method with SEM-DIC and electron backscattering diffraction (EBSD). Thus, in situ mapping of local strain with ultra-high spatial resolution and microstructure in commercially pure titanium during plastic deformation could be achieved, which favored revealing the effect of slip transfer on shear strain near grain boundaries. Moreover, the slip systems could be easily identified via the combination of the SEM-DIC and EBSD techniques even though no obvious deformation trace was captured in secondary electron images. Additionally, the complex geometric compatibility factor $( {m}^{\prime}_c)$ relating to geometric compatibility factors (mʹ) and Schmid factors was proposed to predict the shear strain (εxy) at grain boundaries.

We present a numerical study on how tidal force and topography influence flow dynamics, transport and mixing in horizontal convection. Our results show that local energy dissipation near topography will be enhanced when the tide is sufficiently strong. Such enhancement is related to the height of the topography and increases as the tidal frequency $\omega$ decreases. The global dissipation is found to be less sensitive to the changes in $\omega$ when the latter becomes small and asymptotically approaches a constant value. We interpret the behaviour of the dissipation as a result of the competition among the dominant forces in the system. According to which mechanism prevails, the flow state of the system can be divided into three regimes, which are the buoyancy-, tide- and drag-control regimes. We show that the mixing efficiency $\eta$ for different tidal energy and topography height can be well described by a universal function $\eta \approx \eta _{HC}/(1+\mathcal {R})$, where $\eta _{HC}$ is the mixing efficiency in the absence of tide and $\mathcal {R}$ is the ratio between tidal and available potential energy inputs. With this, one can also determine the dominant mechanism at a certain ocean region. We further derive a power law relationship connecting the mixing coefficient and the tidal Reynolds number.

Light curves of photospheric radius expansion (PRE) bursts, a subset of type I X-ray bursts, have been used as standard candles to estimate the ‘nominal PRE distances’ for 63% of PRE bursters (bursters), assuming PRE burst emission is spherically symmetric. Model-independent geometric parallaxes of bursters provide a valuable chance to test models of PRE bursts (PRE models) and can be provided in some cases by Gaia astrometry of the donor stars in bursters. We searched for counterparts to 115 known bursters in the Gaia Early Data Release 3 and confirmed 4 bursters with Gaia counterparts that have detected ( $\gt\!3\,\sigma$ , prior to zero-point correction) parallaxes. We describe a generic approach to the Gaia parallax zero point as well as its uncertainty using an ensemble of Gaia quasars individually determined for each target. Assuming the spherically symmetric PRE model is correct, we refined the resultant nominal PRE distances of three bursters (i.e. Cen $\textrm{X}-4$ , Cyg $\textrm{X}-2$ , and $4\textrm{U}\,0919-54$ ) and put constraints on their compositions of the nuclear fuel powering the bursts. Finally, we describe a method for testing the correctness of the spherically symmetric PRE model using parallax measurements and provide preliminary results.

The origins of metal coinage and the monetisation of ancient economies have long been a research focus in both archaeology and economic history. Recent excavations of an Eastern Zhou period (c. 770–220 BC) bronze foundry at Guanzhuang in Henan Province, China, have yielded clay moulds for casting spade coins. The technical characteristics of the moulds demonstrate that the site functioned as a mint for producing standardised coins. Systematic AMS radiocarbon-dating indicates that well-organised minting developed c. 640–550 BC, making Guanzhuang the world's oldest-known, securely dated minting site. This discovery provides important new data for exploring the origin of monetisation in ancient China.

We investigate the dynamics of a self-rewetting drop placed on a substrate with a constant temperature gradient via three-dimensional numerical simulations using a conservative level-set approach to track the interface of the drop. The surface tension of a so-called self-rewetting fluid exhibits a parabolic dependence on temperature with a well-defined minimum. Two distinct drop behaviours, namely deformation and elongation, are observed when it is placed at the location of the minimum surface tension. The drop spreads slightly and reaches a pseudo-steady state in the deformation regime, while it continuously spreads until breakup in the elongation regime. Theoretical models based on the forces exerted on the drop have been developed to predict the critical condition at which the drop undergoes the transition between the two regimes, and the predictions are in good agreement with the numerical results. We also investigate the effect of the initial position of the drop with respect to the location of the minimum surface tension on the spreading and migration dynamics. It is found that, at early times, the migration of the drop obeys an exponential function with time, but it diverges at the later stage due to an increase in the drop deformation.

Boasting supreme magnetic strengths, magnetars are among the prime candidates to generate fast radio bursts. Several theories have been proposed for the formation mechanism of magnetars, but have not yet been fully tested. As different magnetar formation theories expect distinct magnetar space velocity distributions, high-precision astrometry of Galactic magnetars can serve as a probe for the formation theories. In addition, magnetar astrometry can refine the understanding of the distribution of Galactic magnetars. This distribution can be compared against fast radio bursts (FRBs) localized in spiral galaxies, in order to test the link between FRBs and magnetars. Swift J1818.0–1607 is the hitherto fastest-spinning magnetar and the fifth discovered radio magnetar. In an ongoing astrometric campaign, we have observed Swift J1818.0–1607 for one year using the Very Long Baseline Array, and have determined a precise proper motion as well as a tentative parallax for the magnetar.

To explore whether different polyvinylpyrrolidone (PVP) concentrations affect the results of intracytoplasmic sperm injection (ICSI), a prospective study was conducted for 194 couples undergoing 210 ICSI therapy cycles. These cycles were divided into three groups (10, 7 and 5% groups) using the corresponding concentration of PVP for sperm immobilization. The main outcome measures were analyzed. Results indicated that, with a decrease in PVP concentrations, all of the main outcome measures increased. In particular, the high-quality cleavage embryo rate in the 7% group was significantly lower than in the 5% group (P < 0.01), and the cleavage, high-quality cleavage embryo, and high-quality blastocyst rates in the 5% group were significantly higher than those in the 10% group (all P < 0.001). For high-/intermediate-quality semen, all of the main outcome measures were significantly increased with 5% PVP. For the poor-quality semen, only the high-quality cleavage embryo and high-quality blastocyst rates were significantly higher in the 5% group. Therefore, lowering PVP concentrations greatly promoted the development of embryos in ICSI cycles, with an optimal concentration of 5% for ICSI.

We numerically investigate the mechanism resulting in fate change of a hydrophobic sphere impacting onto a confined pool, that is, at the same impact speed, it does not submerge in a wide pool but does in a narrow pool. We find that the reflection of the impact-induced gravity-capillary waves from the pool boundary is responsible for this phenomenon. In particular, the return of the wave to the symmetry axis may coincide with the rising of the impacting sphere to the water surface, which corresponds to the critical conditions of the fate change. Moreover, for the spheres at the onset of submersion in a wide pool, our analysis suggests that this scenario also accounts for an interesting observation in the numerical simulations. That is, the effective pool size $S_{c}$, beyond which the submersion of impacting spheres is no longer affected by the pool size $S$, is mainly dependent on the sphere diameter, no matter whether the surface waves are the capillary or gravity waves. For $S<S_{c}$, two important pool sizes ($S_{w,1}$ and $S_{w,2}$, and $S_{w,2}\geqslant S_{w,1}$) are identified at the same impact speed, and the sphere submersion takes place at $S_{w,1}\leqslant S\leqslant S_{w,2}$. Based on the flow features identified in simulations, a scaling law is proposed to correlate the Weber number and Bond number with $S_{w}$. The theoretical prediction is shown to agree well with the numerical results.

We report direct numerical simulation results that clearly elucidate the mechanism that leads to curvature dependence of drag enhancement (DE) in viscoelastic turbulent Taylor–Couette flow. Change in the angular momentum transport and its inherent link to transitions in vortical flow structures have been explored to depict the influence of the curvature of the flow geometry on DE. Specifically, it has been demonstrated that a transition in vortical structures with increasing radius ratio leads to weakening and elimination of the small-scale Görtler vortices and development and better organization (occupying the entire gap) of large-scale Taylor vortices as also evinced by the patterns of angular momentum current. The commensurate change in DE and its underlying mechanism are examined by contributions of convective flux and polymeric stress to the angular momentum current. The present finding paves the way for capturing highly localized elastic turbulence structures in direct numerical simulation by increasing geometry curvature in traditional turbulent curvilinear flows.

In January 2016, static GPS measurements were carried out in a 30 × 30 km2 area centered around Kunlun station at Dome Argus (Dome A), East Antarctica, to acquire high-precision 3-D geodetic coordinates at 49 sites. By comparing the coordinates with previous GPS measurements in 2008 and 2013 at the same sites, we constructed a detailed and long-term record of the ice-surface velocity field, 2008–2016, around Dome A. During this time span, the estimated ice-surface velocity ranges from 0.8 ± 0.3 to 28.7 ± 1.6 cm a−1, with a mean of 10.4 ± 0.3 cm a−1. From 2013 to 2016, the surface elevation of most Dome A areas exhibits a rising trend, and the maximum increase of snow surface elevation is 84.8 cm. The mean snow surface elevation change rate at Dome A is estimated to be 6.6 ± 0.7 cm a−1. The difference of 1.0 cm a−1 between the snow surface change rate derived from GPS and pole-height change rate from surface mass balance is suspected to be a result of a combination of firn densification and basal melt under Dome A.

Using repeat GPS measurements during 2005–16, we calculated and updated two-dimensional high-resolution decadal ice surface velocity estimates along the traverse route from Zhongshan Station to and around Dome Argus, East Antarctica. Along the 71 sites of the transect, the magnitudes of ice velocity increased from near 0 in Dome Argus to 1, 10 and ~100 m a−1 at the sites DT416, DT333 and LT980, respectively. The comparison between GPS and interferometric synthetic aperture radar (InSAR) derived results agree well when the magnitude of the ice surface velocities is faster than 5 m a−1, and disagree for slower flow velocities. A scale value 1.15 and 0.12 can be applied to InSAR derived results over this region with ice surface velocity larger and <5 m a−1, respectively. We attributed the cause of the discrepancy to the insensitivity of InSAR to the magnitude of low ice surface velocities, thus confirming the importance of GPS fieldwork-based ground truth high-resolution ice velocity estimates to constrain ice-sheet dynamics.

We numerically investigate the mechanism leading to the entrapment of spheres at the gas–liquid interface after impact. Upon impact onto a liquid pool, a hydrophobic sphere is seen to follow one of the three regimes identified in the experiment (Lee & Kim, Langmuir, vol. 24, 2008, pp. 142–145): sinking, bouncing or being entrapped at the interface. It is important to understand the role of wettability in this process of flow–structure interaction with dynamic wetting, and in particular, to what extent the wettability can determine whether the sphere is entrapped at the interface. For this purpose, a diffuse-interface immersed boundary method is adopted in the numerical simulations. We expand the parameter space considered previously, provide the phase diagrams and identify the key phenomena in the impact dynamics. Then, we propose the scaling models to interpret the critical conditions for the occurrence of sphere entrapment, accounting for the wettability of the sphere. The models are shown to provide a good correlation among the impact inertia of the drop, the surface tension, the wettability and the density ratio of the sphere to the liquid.

In this paper, drop impact onto a sphere is numerically investigated at moderate Reynolds and Weber numbers. It is naturally expected that the aspect ratio of the sphere to the drop, $\unicode[STIX]{x1D706}_{r}$, would make a big difference to drop spreading and retraction on the sphere, compared with drop impact onto a flat substrate. To quantitatively assess the effect of $\unicode[STIX]{x1D706}_{r}$, a diffuse-interface immersed-boundary method is adopted after being validated against experiments. With the help of numerical simulations, we identify the key regimes in the spreading and retraction, analyse the results by scaling laws, and quantitatively evaluate the effect of $\unicode[STIX]{x1D706}_{r}$ on the impact dynamics. We find that the thickness of the liquid film spreading on the sphere can be well approximated by $h_{L,\infty }(1+3/4\unicode[STIX]{x1D706}_{r}^{-3/2})$, where $h_{L,\infty }$ represents the film thickness of drop impact on a flat substrate. At the early stage of spreading, the temporal variation of the wetted area is independent of $\unicode[STIX]{x1D706}_{r}$ when the time is rescaled by the thickness of the liquid film. Drops are observed to retract on the sphere at a roughly constant speed, and the predictions of theoretical analysis are in good agreement with numerical results.

We report on a target system supporting automated positioning of nano-targets with a precision resolution of $4~\unicode[STIX]{x03BC}\text{m}$ in three dimensions. It relies on a confocal distance sensor and a microscope. The system has been commissioned to position nanometer targets with 1 Hz repetition rate. Integrating our prototype into the table-top ATLAS 300 TW-laser system at the Laboratory for Extreme Photonics in Garching, we demonstrate the operation of a 0.5 Hz laser-driven proton source with a shot-to-shot variation of the maximum energy about 27% for a level of confidence of 0.95. The reason of laser shooting experiments operated at 0.5 Hz rather than 1 Hz is because the synchronization between the nano-foil target positioning system and the laser trigger needs to improve.