In teleosts, vitellogenin (Vtg) is a phospholipoglycoprotein synthesized by the liver, released into the blood circulation and incorporated into the oocytes via endocytosis mediated by the Vtg receptor (VTGR) to form the yolk granules. The VTGR is crucial for oocyte growth in egg-laying animals but is also present in non-oviparous vertebrates, such as human. The VTGR belongs to the low-density lipoprotein receptor superfamily (LDLR) and is also named very-low-density lipoprotein receptor (VLDLR). In this study, we identified and phylogenetically positioned the VTGR of a basal teleost, the European eel, Anguilla anguilla. We developed quantitative real-time PCR (qRT-PCR) and investigated the tissue distribution of vtgr transcripts. We compared by qRT-PCR the ovarian expression levels of vtgr in juvenile yellow eels and pre-pubertal silver eels. We also analyzed the regulation of ovarian vtgr expression throughout vitellogenesis in experimentally matured eels. The Vtg plasma level was measured by homologous ELISA experimental maturation. Our in silico search and phylogenetical analysis revealed a single vtgr in the European eel, orthologous to other vertebrate vtgr. The qRT-PCR studies revealed that vtgr is mainly expressed in the ovary and also detected in various other tissues such as brain, pituitary, gill, fat, heart, and testis, suggesting some extra-ovarian functions of VTGR. We showed that vtgr is expressed in ovaries of juvenile yellow eels with no higher expression in pre-pubertal silver eels nor in experimentally matured eels. This suggests that vtgr transcription already occurs during early pre-vitellogenesis of immature eels and is not further activated in vitellogenic oocytes. European eel Vtg plasma level increased throughout experimental maturation in agreement with previous studies. Taken together, these results suggest that vtgr transcript levels may not be a limiting step for the uptake of Vtg by the oocyte in the European eel.

The synthesis and texturization processes of fluorinated surfaces by means of atmospheric plasma are investigated and presented through an integrated study of both the plasma phase and the resulting material surface. Three methods enhancing the surface hydrophobicity up to the production of super-hydrophobic surfaces are evaluated: (i) the modification of a polytetrafluoroethylene (PTFE) surface, (ii) the plasma deposition of fluorinated coatings and (iii) the incorporation of nanoparticles into those fluorinated films. In all the approaches, the nature of the plasma gas appears to be a crucial parameter for the desired property. Although a higher etching of the PTFE surface can be obtained with a pure helium plasma, the texturization can only be created if O2 is added to the plasma, which simultaneously decreases the total etching. The deposition of CxFy films by a dielectric barrier discharge leads to hydrophobic coatings with water contact angles (WCAs) of 115°, but only the filamentary argon discharge induces higher WCAs. Finally, nanoparticles were deposited under the fluorinated layer to increase the surface roughness and therefore produce super-hydrophobic hybrid coatings characterized by the nonadherence of the water droplet at the surface.

The objective of this study was to determine the distribution of Shiga toxin-producing Escherichia coli (STEC) virulence markers (stx1, stx2, eae, ehxA) in E. coli strains isolated from young calves aged fewer than 7 days (bobby calves). In total, 299 recto-anal mucosal swabs were collected from animals at two slaughter plants and inoculated onto tryptone bile X-glucuronide and sorbitol MacConkey agar supplemented with cefixime and potassium tellurite. Isolates were analysed using multiplex polymerase chain reaction to detect stx1, stx2, eae and ehxA genes. The most common combination of virulence markers were eae, ehxA (n = 35) followed by eae (n = 9). In total, STEC and atypical enteropathogenic E. coli (aEPEC) were isolated from 8/299 (2·6%) and 37/299 (12·3%) calves, respectively. All the isolates could be assigned to 15 genotype clusters with >70% similarity cut-off using XbaI pulsed-field gel electrophoresis. It may be concluded that healthy calves from the dairy industry are asymptomatic carriers of a diverse population of STEC and aEPEC in New Zealand.

NASA's NuSTAR observatory is the first focusing hard X-ray telescope. Launched in June 2012, NuSTAR is sensitive in the 3–79 keV range with unprecedented ~17″ FWHM angular resolution above 12 keV, a result of its multilayer-coated optics and 10-m focal length. With its large effective area (900 cm2 at 10 keV), NuSTAR has point-source sensitivity ~100 times better than previous hard X-ray telescopes. Here we describe NuSTAR and its planned work on rotation-powered pulsars and magnetars during its nominal 2-yr baseline mission that has just commenced.

We discuss the 3D morphology, ionization structure, and kinematics of NGC 2392, the “Eskimo,” based on new and archival HST imagery and new long-slit echelle spectroscopy. High spatial resolution ionization maps of the nebula were made from HST WFPC2 imagery and compared with their velocity structure in various emission lines from echelle spectra taken with the 4m telescope at Kitt Peak. The imagery and spectra were then compared to map the kinematics of the nebula in several emission lines and decode the 3-dimensional morphology and ionization structure of the nebula, including that of C+2 from C III] 1909 Å for the first time.

The objective was to study the multidimensional nature of the relationship between adult obesity (OB) and socio-economic status (SES), using comprehensive indices of SES taken separately or synthesised in an overall index. A nationally representative sample of adults aged 18–79 years was taken from the French second National Individual Survey on Food Consumption (INCA 2) dietary survey (2006–07). Weight and height were measured and OB defined as BMI ≥ 30 kg/m2. SES variables were reported in questionnaires and included occupation, education and characteristics of household wealth. Composite indices of SES (household wealth and overall SES indices) were computed by correspondence analysis, and relationships with OB were investigated with logistic regression analysis. In total, 11·8 (95 % CI 10·1, 13·4) % of French adults were obese, without significant difference by sex. While no significant relationship was observed in men, all SES indicators were inversely correlated to OB in women. Both education and the household wealth index were retained in the stepwise multivariate model, confirming that different socio-economic variables are not necessarily proxies of each other regarding the OB issue. On the other hand, ‘controlling for SES’ while including several measures of SES in multivariate models may lead to collinearity, and thus over-adjustment. A more integrative approach may be to derive a synthetic index by including the SES factors available in a given study. Beyond this methodological perspective, understanding how OB is related to the different dimensions of SES should help to target the more vulnerable groups and increase the effectiveness of prevention.

The laser program developed at the Centre d'Etudes de Limeil-Valenton, Saint-Georges, France (CEL-V) is concentrated on a systematic investigation of indirect drive fusion; by comparison with direct drive, this process is expected to provide the required irradiation uniformity with relaxed constraints on laser beam quality. The main concerns are radiative transfer and preheat, hydrodynamic instabilities, and high-density X-ray driven implosions. Ablative implosion experiments have been conducted with the two beams at the Phebus facility (5 kJ, 1.3 ns, 0.35 μm). Symmetry was proved to be controlled by the casing structure, following scaling laws describing hohlraum physics. A compressed DT density ∼100 ρ0 (ρ0 liquid DT density) has been deduced from activation measurements. Different aspects of the soft X-ray transfer processes, and particularly of the ablation of a low-Z material, which drives the capsule implosion, are dealt with in detailed investigations. Reported here are results on X-ray reemission and penetration in several materials, and on induced hydrodynamics of accelerated foils. The laser energy required to reach fuel ignition conditions has been evaluated from numerical simulations as well as from analytical models, taking into account hohlraum physics, capsule implosion, hot spot formation, and burn propagation. Several crucial parameters have been drawn, the most important being the radiation temperature. A target gain in the order of 10 appears achievable with a 2-MJ laser.

Implosion experiments performed at Centre d'Etudes de Limeil-Valenton in the indirect drive scheme using the two-beams Nd:glass laser facility Phebus at the energy level = 6 kJ (blue light) are presented. A final density of compressed DT close to 100 ρ0 has been obtained; it has been deduced from radiochemistry of the activated silicon atoms in the pusher. The best irradiance uniformity on the microballoon was evaluated to = 15% rms. Phebus has also been equipped with an optical fiber oscillator to study the effect of a smoothing technique on coupling processes: It appeared that at 0·53 μm absorption efficiency is increased by =15–20%. With the eight-beams Octal laser, hydrodynamic instabilities development in accelerated planar targets has been investigated both for direct and indirect drives; the mixing zone detected at the light-heavy interface does not present visible bubble-and-spike like structures and is less developed in the indirect configuration. Atomic physics in laser plasmas is also deeply studied; a particular effort has been made on absorption spectroscopy, a powerful diagnostic of ionization dynamics in cold and dense plasmas. Experiments have been realized either in multilayered targets or using rear-side X-ray emission of thin Au foils to heat the samples. To reach fuel ignition conditions, more powerful lasers, in the range of megajoule, will be needed. Their design needs further technological developments to reduce the capital cost in $/W. At Limeil, we work mainly on high-damage threshold optical coatings, using the sol-gel process, high-quality, low-cost mirror fabrication, using the replica technics, and incoherent laser pulse generation for beam smoothing.

Emission-line observations made with the Spitzer Space Telescope are used to test the predicted ionizing spectral energy distributions of various stellar atmosphere models.

Emission-line observations made with the Spitzer Space Telescope are used to test the predicted ionizing spectral energy distributions of various stellar atmosphere models.

We obtained new HST/STIS long-slit spectra and WFPC2 imagery of the planetary nebula NGC 7009 in order to obtain high spatial resolution of the intrinsic flux ratio [O III] 4364/5008, which is a well-known diagnostic for electron temperature (Te). Our primary purpose was to quantify Te variations across the nebula. We address whether the observational data support the possibility that the [fractional] mean-square Te variation (t2) (Peimbert 1967) in NGC 7009 may be as large as ~0.1. Such large values are required to reconcile the “abundance dichotomy” by Te variations alone. The abundance dichotomy (discussed by Liu at greater length elsewhere in this volume) refers to the significantly higher heavy element abundances derived from optical recombination lines (e.g., a factor of ~5 for NGC 7009, Liu et al. 1995) compared with the corresponding values deduced from collisionally-excited lines.

Unless otherwise explicitly stated all mappings and tensors in the paper are C∞. A Poisson structure on a (C∞) manifold M is a bracket operation (f, g) [map ] {f, g}, on the set of functions on M, which gives to this set a Lie algebra structure and which verifies the relation

formula here

An equivalent way to get such a structure is to give a 2-vector (that is, an antisymmetric two times contravariant tensor) P satisfying

formula here

where [,] is the Schouten bracket [7]. We then have

formula here

This paper is devoted to the local classification of these structures. The decomposition theorem of A. Weinstein [8, 9] reduces the problem to the case where P is a Poisson structure on Rn which vanishes at zero.

In this paper we will denote by PS(n) the set of germs at zero of Poisson structures on Rn vanishing at the origin.

In the previous design, the maximum drive radiation temperature was 4 MK or 350 eV (Holstein 1996). Different beam configurations gave roughly the same uniformity with the NIF-size cavity. Our best configuration used four cones of beams illuminating three rings. An integrated 2D simulation pointed out that the symmetry was good enough to reach a gain of ten. Two evolutions took place in the design of our MJ laser. We moved from a capsule adapted to 4 MK (L1000) to another one adapted to 3.5 MK (L1215) in order to minimize the parametric instabilities (the cavity size is almost the same). This new capsule also has a better hydrostability according to the “classical modelling” (Lindl 1995). The second evolution is a simplification of the target chamber. We restricted ourselves to two major configurations for indirect drive (two-ring and three-ring configurations). Therefore, only three cones of beams are necessary instead of five cones in the first design. Finally, the number of holes in the chamber is 80 instead of 100.

The halo planetary nebulae are of special interest insofar as the sample is very limited and they show a wide range of nebular chemical properties. We are interested in deriving their detailed nebular properties and to try to determine the characteristics of their progenitor stars. We selected M2-29 and DDDM-1 since they are the most deficient in Ar and S of their group. During HST Cycle 3 we obtained WFC1 direct imagery in Hα of them to derive information on their morphology and surface brightness for performing FOS spectroscopy.

The Rare-Earth Laves phases RE-Fe2 (RE represent the Rare-Earth) show large magnetostrictive properties, especially at room temperature. These materials are well characterized when in bulk form, but they have rarely been studied as thin films and one can expect some important effects due to epitaxial growth.

A few single crystal layers of RE-Fe2 have been studied (YFe2, TbFe2, DyFe2, ErFe2: and Dy0.7Tb0.3Fe2 known as Terfenol-D). The thickness of these different layers are between 5 and 20 nm and with [110] as a growth direction have been epitaxied. They have been deposited with a Molecular Beam Epitaxy (MBE) in an ultra high vacuum chamber. A [1120] sapphire substrate is recovered by a [110] niobium buffer. The RE and the iron are then co-deposited on the substrate which is maintained at 500°C. Lastly, an Yttrium layer is deposited on the Rare Earth material at a temperature close to ambient.

We assess the gas-phase abundances of Si, C, and Fe from our recent measurements of Si++, C++, and Fe++ in the Orion Nebula by expanding on our earlier “blister” models. The Fe++ 22.9 μm line measured with the KAO yields Fe/H ~ 3 × 10−6 - considerably larger than in the diffuse ISM, where relative to solar, Fe/H is down by ~ 100. However, in Orion, Fe/H is still lower than solar by a factor ~ 10. The C and Si abundances are derived from new IUE high dispersion spectra of the C++ 1907, 1909 Å and Si++ 1883, 1892 Å lines. Gas-phase Si/C = 0.016 in the Orion ionized volume and is particularly insensitive to uncertainties in extinction and temperature structure. The solar value is 0.098. Gas-phase C/H = 3 × 10−4 and Si/H = 4.8 × 10−6. Compared to solar, Si is depleted by 0.135 in the ionized region, while C is essentially undepleted. This suggests that most Si and Fe resides in dust grains even in the ionized volume.

The study of diffuse celestial sources in the ground-inaccessible ultraviolet spectral range is less advanced than UV studies of point and compact sources. The main reason is that the characteristics of instrumentation optimized for the two types of objects are quite different. Studies of diffuse objects are best made with fast focal ratio optics with wide fields of view, whereas studies of point and compact objects are best made with large telescope aperture and high angular resolution. As a result, most space ultraviolet instruments to date (such as the International Ultraviolet Explorer and the forthcoming Hubble Space Telescope) are not well suited to the study of faint, extended diffuse objects in the ultraviolet.

The efficacy of antidepressants is measured primarily by their ability to treat the acute symptoms of depression yet the underlying process may take longer to resolve. A period of emotional frailty follows resolution of the acute stage, during which the patient, although apparently symptom-free, is prone to suffer relapse of the original symptoms. Antidepressants should not be stopped immediately a response is observed, but should be given for a longer period to prevent early relapse. In those suffering from recurrent depression, long-term treatment may prevent later recurrences of new episodes of depression.

The distinction between relapse of old symptoms and recurrence of new episodes is of particular importance, both in assessing efficacy of antidepressants during the continuation phase of acute treatment and in the separate assessment of their prophylactic efficacy. Unfortunately, though, many investigations have failed to distinguish between these two distinct phenomena. Klerman & Paykel (1970) emphasised the need to distinguish between early return of depressive symptoms, which they label ‘relapses’, from the later new episodes which they term ‘recurrence’. However, there has been a general lack of systematic investigation of this question, as well as a failure to use sufficiently precise methodology to distinguish between relapse and recurrence. Some studies which purported to be of prophylaxis were in effect only examining the continuation phase of acute treatment.

Our Present Understanding of the Mechanisms of the transformation of SiO films under treatments in O2 or Ar plasma at room T, is presented. RBS, IR absorption, XPS, RHEED and TEM techniques have been used to investigate the composition, the chemical and structural nature of the films after oxidizing or non oxidizing treatments. In O2 plasma, SiO is converted into Si02 by room T plasma anodization. The initial step of SiO oxidation, which is believed to be the transformation 2SiO→Si+SiO2, was studied in Ar plasma. We show that the as deposited SiO (not a mixture of Si and SiO2) is subject to disproportionate into the later compounds and that the effects of irradiation by low energy electrons at room T (plasma treatment) are similar to those observed for high T treatments (rapid thermal annealing).

A total of 177 sows representing five two-breed crosses were used in a 2×2 factorial experiment to study the effects of post-weaning stress (changing environment and group housing) and feeding regime (flushing by feeding ad libitum until mating) on the weaning-oestrus interval and other reproductive traits up to 30 days of pregnancy.

The group which was stressed had about 10% (P<0·05) fewer reproductive failures than those not stressed, while little difference was found between the two feeding regimes. About 61% of the flushed sows returned to oestrus within 7 days after weaning, 9% higher than for those not flushed. The stress treatment had little effect on the weaning-oestrus interval. Neither treatment affected ovarian and follicular-fluid weights, percentage of follicles of different sizes, ovulation rate nor number of viable embryos. The flushed group, however, had 9% lower embryo survival than the group not flushed. A positive relation was found between the number of days on ad libitum feeding and ovulation rate and embryonic mortality.

Differences among breeds were significant for all the traits, except weaning-oestrus interval, weight of follicular fluids, percentage of large follicles, ovulation and survival rates. The weaning-oestrus interval was not significantly correlated with any of the reproductive traits studied.