At coastal archaeological sites, measuring erosion rates and assessing artifact loss are vital to understanding the timescale(s) and spatial magnitude of past and future site loss. We describe a straightforward low-tech methodology for documenting shoreline erosion developed by professionals and volunteers over seven years at Calusa Island Midden (8LL45), one of the few remaining sites with an Archaic component in the Pine Island Sound region of coastal Southwest Florida. We outline the evolution of the methodology since its launch in 2016 and describe issues encountered and solutions implemented. We also describe the use of the data to guide archaeological research and document the impacts of major storms at the site. The response to Hurricane Ian in 2022 is one example of how simply collected data can inform site management. This methodology can be implemented easily at other coastal sites at low cost and in collaboration with communities, volunteers, and heritage site managers.

In this article we ‘read against the grain’ of the archive to explore the sound world of an Indigenous rebellion against Spanish colonial rule in Chiapas in 1712. Although this topic has often interested historians and anthropologists, none of them explicitly engage the rebellion’s sonorities. Contending that a focus on sound may allow new features of the rebellion to come to light, we explore the use of sonorous objects and musical instruments in the rebels’ religious worship and military practices. Building on this analysis, we emphasize the place of ideas about ‘quietude’ within practices for violently reasserting colonial power.

This article reflects on a little-told story of musical and cultural censorship: the Mexican government's 1996 destruction of the Foro Abierto, a 2,000-seater auditorium in the heart of Chapultepec Park in Mexico City. The Foro Abierto was the home of an anarchist theatre group called CLETA, and was an important venue for a number of genre-crossing musicians. This venue was destroyed by the police just before CLETA was to inaugurate it as part of the rebel Zapatista movement. Several days later, a musician from CLETA was assassinated. Responding to the material turn in music studies, this article combines ethnographic and archival research to explore articulations between rubble and censorship. The venue's destruction disempowered and disarticulated CLETA, to the extent that an unsettling silence emerged about this act on the site of the venue itself. Equally, constructive responses to the complexities of censorship may also emerge from acts of material and ideational de-structuration.

Tightly focused proton beams generated from helical coil targets have been shown to be highly collimated across small distances, and display characteristic spectral bunching. We show, for the first time, proton spectra from such targets at high resolution via a Thomson parabola spectrometer. The proton spectral peaks reach energies above 50 MeV, with cutoffs approaching 70 MeV and particle numbers greater than 10${}^{10}$. The spectral bunch width has also been measured as low as approximately 8.5 MeV (17% energy spread). The proton beam pointing and divergence measured at metre-scale distances are found to be stable with the average pointing stability below 10 mrad, and average half-angle beam divergences of approximately 6 mrad. Evidence of the influence of the final turn of the coil on beam pointing over long distances is also presented, corroborated by particle tracing simulations, indicating the scope for further improvement and control of the beam pointing with modifying target parameters.

A battery of 32 tests was administered to a sample including 144 Air Force Officer Candidates and 139 Air Cadets. The factor analysis, using Thurstone's complete centroid method and Zimmerman's graphic method of orthogonal rotations, revealed 12 interpretable factors. The non-reasoning factors were interpreted as verbal comprehension, numerical facility, perceptual speed, visualization, and spatial orientation. The factors derived from reasoning tests were identified as general reasoning, logical reasoning, education of perceptual relations, education of conceptual relations, education of conceptual patterns, education of correlates, and symbol substitution. The logical-reasoning factor corresponds to what has been called deduction, but eduction of correlates is perhaps closer to an ability actually to make deductions. The area called induction appears to resolve into three eduction-of-relations factors. Reasoning factors do not appear always to transcend the type of test material used.

In the application of clustering methods to real world data sets, two problems frequently arise: (a) how can the various contributory variables in a specific battery be weighted so as to enhance some cluster structure that may be present, and (b) how can various alternative batteries be combined to produce a single clustering that “best” incorporates each contributory set. A new method is proposed (SYNCLUS, SYNthesized CLUStering) for dealing with these two problems.

This proof-of-concept study evaluated an optimization strategy for the Community Case Detection Tool (CCDT) aimed at improving community-level mental health detection and help-seeking among children aged 6–18 years. The optimization strategy, CCDT+, combined data-driven supervision with motivational interviewing techniques and behavioural nudges for community gatekeepers using the CCDT. This mixed-methods study was conducted from January to May 2023 in Palorinya refugee settlement in Uganda. We evaluated (1) the added value of the CCDT+ in improving the accuracy of detection and mental health service utilization compared to standard CCDT, and (2) implementation outcomes of the CCDT+. Of the 1026 children detected, 801 (78%) sought help, with 656 needing mental health care (PPV = 0.82; 95% CI: 0.79, 0.84). The CCDT+ significantly increased detection accuracy, with 2.34 times higher odds compared to standard CCDT (95% CI: 1.41, 3.83). Additionally, areas using the CCDT+ had a 2.05-fold increase in mental health service utilization (95% CI: 1.09, 3.83). The CCDT+ shows promise as an embedded quality-optimization process for the detection of mental health problems among children and enhance help-seeking, potentially leading to more efficient use of mental health care resources.

The number of people following a plant-based (PB) diet has increased in recent years. This has led to concerns regarding vitamin D intake, as natural dietary sources of vitamin D are primarily animal based(1) and cross-sectional studies have reported lower intakes amongst vegetarians and vegans(2). Some PB foods, primarily dairy alternatives, are now fortified with vitamin D. Whilst vitamin D is synthesised in the skin following exposure to UV light, this does not happen in the autumn and winter months in areas of northern latitude (such as the UK). Hence, dietary supply of vitamin D becomes particularly important. This study aimed to investigate whether it is possible to maintain dietary vitamin D intake when transitioning to a PB diet, through careful choice of PB alternatives.

A subset of 4-day diet diaries, from 81 women (72 Caucasian and 9 South Asian, mean age 52 ± 12y) living in South East England, was randomly selected from those collected for the D-FINES study (Vitamin D, Food Intake, Nutrition and Exposure to Sunlight in Southern England, 2006–2007; FSA funded Project N05064, NHS REC 06/Q1909/1)(3)and analysed for vitamin D intake. No subjects followed an entirely PB diet at baseline. Data modelling was carried out to substitute animal-based foods with equivalent amounts of PB alternatives. For dairy products, the equivalent fortified PB alternatives were chosen. Meat, fish and eggs were replaced with equivalent amounts of protein from PB sources, such as lentils and tofu, as no fortified direct alternatives were found. The diaries were then re-analysed. Pre- and post-substitution intakes were compared by paired t test, and ethnic groups by independent t test.

There were no differences in Vitamin D intake between ethnicities either at baseline (p = 0.087) or post-substitution (p = 0.361). Vitamin D intake increased in the South Asian group post-substitution (from 2.4 ± 1.3 to 4.3 ± 1.8 µg/day, p = 0.002), with no change observed in the Caucasian group (3.6 ± 2.0 to 3.7 ± 1.8 µg/day, p = 0.660). There was no difference between vitamin D intake pre- and post-substitution overall (3.5 ± 2.0 and 3.8 ± 1.8 µg/day respectively, p = 0.222). Twenty cases decreased by ≥1 µg/day, all in the Caucasian group, with the largest decrease being from 9.6 to 2.2 µg/day.

This study demonstrates that it is possible to move to a PB diet, whilst maintaining existing dietary patterns, without a reduction in vitamin D intake, but it is largely dependent on dairy intake in the original diet, as no meat/fish substitutes in the UK are currently fortified. It also requires care when choosing PB dairy substitutions as many, including organic options, are not fortified. Mean daily intakes, both pre- and post-substitution, were substantially below the recommended intake of 10 µg/day(4), suggesting that supplementation may be necessary, particularly during winter months.

This abstract was presented as the Novel Nutrition Research Methodologies and Technologies

Theme Highlight. Queuosine (Q) is a vital micronutrient crucial for protein translation and cellular function in humans. Deficiencies of Q have been linked to chronic illnesses, such as inflammatory bowel disease, neurodegenerative diseases, and various cancers. Human requirements for this micronutrient have not yet been established. Thus far, there has been no systematic investigation of the human diet to understand typical intakes. It is necessary to measure the levels of Q metabolites in commonly consumed foods to identify the richest dietary sources and determine Q intake. Only then can its role in human health and disease be established.

This study aimed to comprehensively analyse Q distribution in the UK diet by quantifying it in foods, identifying rich sources, and ultimately, enabling the design of dietary intervention studies assessing Q absorption/bioavailability.

Data from the UK National Diet and Nutrition Survey (NDNS 5Y) were processed to identify the most frequently consumed foods in 31 main categories, reflecting the dietary habits of >99.5% of UK consumers. Extraction techniques were developed, including a methanol-based method for extracting free Q metabolites and a specialized approach for isolating tRNA-bound Q metabolites from various food matrices. Quantitative LC-MS/MS (SCIEX 5500+) was utilized to accurately measure queuine (q), Q, Q monophosphates (QMPs), and Q precursor (PreQ1) in food samples. These values were summed to determine Total Q levels. Additionally, four-day food diary data from 93 participants in the Northern Ireland Dietary Assessment Study (NIDAS) were analysed to determine the average daily consumption of Q metabolites in adults. Dairy sources were considered promising, so five commercial probiotic yoghurt brands were compared side-by-side.

Q metabolite levels varied significantly across various food types. Total Q metabolite levels in foods ranged from 0.05ng/g to 80ng/g, with a mean level of 20ng/g. The calculated Q consumption of participants in NIDAS ranged from nil/day in some individuals to 170µg/day in others, with a typical mean intake of 18µg/day. The mean amounts (ng/g) of Total Q metabolites (tRNA-bound + free) measured in food categories were dairy 57.2 ± 9.7, meat 20.1 ± 9.9, poultry 4.9 ± 4, and plant-based milk 15.3 ± 10, fruits 2 ± 1.3, vegetables 5.3 ± 2.8, starchy foods 2.2 ± 0.7. Dairy products emerged as significant sources of Q metabolites based on these findings. Concentrations observed in shortlisted probiotic yoghurts ranged from 32 to 352 ng/g.

This study determined typical Q intakes and is the first to identify Q-rich food sources in the UK diet. The highly Q-enriched probiotic yoghurt products could be used in controlled dietary intervention studies to assess Q absorption/bioavailability and this micronutrient’s role in human health and disease.

Heat stress from high temperatures has been recognised as a threat to several bird species and one that is likely to increase in severity and frequency as a result of global environmental change. Many seabirds are particularly vulnerable as their adaptations to forage in cold water can make it hard for them to resist heat gain while on land. The African Penguin Spheniscus demersus forages in cool water (10–20°C) but breeds and moults on land where temperatures can exceed 30°C. Little is known about its thermal physiology and when heat stress will occur. Here, we provide the first account of changes in body temperature and behaviour in African Penguins in response to an experimental increase in ambient temperature from 20°C to 35°C. Surprisingly, the highest body temperature observed (39.2°C) was recorded at the start of the study. Presumably, this response in body temperature was due to the stress of transport and handling. Penguins returned to normal body temperature (37.3°C) after 3.5 hours and maintained normothermia for roughly an hour. We then observed the onset of heat storage at an ambient temperature of 29°C, whereafter body temperature increased steadily at a rate of ca.0.2°C per 1°C increase in ambient temperature. Panting commenced at an ambient temperature of 31.4°C, when body temperature was 37.8°C. In addition to panting, penguins changed their posture to facilitate heat dissipation by standing, raising their heads, and extending their flippers. Our results corroborate field observations and support the argument that African Penguins are highly vulnerable to heat stress in the near future as extreme heat events become more severe and frequent due to climate change. Our results also confirm that penguins are sensitive to handling, which elicits a hyperthermic response. Given the general sensitivity of penguins to disturbance, from a conservation perspective, we advise that cautionary measures be implemented at colonies during critical life-history stages.

Dyads can be challenging to recruit for research studies, but detailed reporting on strategies employed to recruit adult–adolescent dyads is rare. We describe experiences recruiting adult–youth dyads for a hypertension education intervention comparing recruitment in an emergency department (ED) setting with a school-based community setting. We found more success in recruiting dyads through a school-based model that started with adolescent youth (19 dyads in 7 weeks with < 1 hour recruitment) compared to an ED-based model that started with adults (2 dyads in 17 weeks with 350 hours of recruitment). These findings can benefit future adult–youth dyad recruitment for research studies.

The thermal reaction sequence of a synthetic hectorite (Laponite CP) was studied by X-ray diffraction, i.r. spectroscopy and thermal analysis. Although most of the interlayer water is removed at 200°C, a smally steady weight loss occurs until dehydroxylation is complete at about 700°C, indicating that an anhydrous intermediate phase is not formed prior to dehydroxylation. Immediately after dehydroxylation, enstatite and cristobalite can be identified, but lithium silicates are formed only from lithium-saturated hectorite. Around 1200°C a glass is formed by reaction of the alkalis with cristobalite, and removal of silica from the enstatite produces some forsterite. An inhomogeneous mechanism of dehydroxylation is postulated by analogy with that proposed for talc.

Adsorption of clay particles from very dilute aqueous suspensions onto positively charged films (Cytochrome C) on carbon coated support grids has been used to obtain well dispersed samples of the particles suitable for detailed electron microscopic examination. The natural negative charge of the carbon coated grid was used for ferric polycations and precipitates.

Laser-driven neutron sources are routinely produced by the interaction of laser-accelerated protons with a converter. They present complementary characteristics to those of conventional accelerator-based neutron sources (e.g. short pulse durations, enabling novel applications like radiography). We present here results from an experiment aimed at performing a global characterization of the neutrons produced using the Titan laser at the Jupiter Laser Facility (Livermore, USA), where protons were accelerated from 23 $\mathrm {\mu }$m thick plastic targets and directed onto a LiF converter to produce neutrons. For this purpose, several diagnostics were used to measure these neutron emissions, such as CR-39, activation foils, time-of-flight detectors and direct measurement of $^7{\rm Be}$ residual activity in the LiF converters. The use of these different, independently operating diagnostics enables comparison of the various measurements performed to provide a robust characterization. These measurements led to a neutron yield of $2.0\times 10^{9}$ neutrons per shot with a modest angular dependence, close to that simulated.

As the federal government continues to expand upon and improve its data sharing policies over the past 20 years, complex challenges remain. Our interviews with U.S. academic genetic researchers (n=23) found that the burden, translation, industry limitations, and consent structure of data sharing remain major governance challenges.

Contemporary understanding of the mechanisms of disease increasingly points to examples of “genetic diseases” with an infectious component and of “infectious diseases” with a genetic component. Such blurred boundaries generate ethical, legal, and social issues and highlight historical contexts that must be examined when incorporating host genomic information into the prevention, outbreak control, and treatment of infectious diseases.