Objectives/Goals: Research supports the use of music to improve the care and well-being of adults living with dementia; however, the practice and implementation of music in elder care communities is not regulated. The goal of this qualitative study was to survey elder care communities in Northeast Kansas to determine the use of music with people living with dementia. Methods/Study Population: We interviewed staff (n = 10) at five elder care communities in the Kansas City Metro area and observed musical activities and artifacts in shared living spaces within each community. Interview questions included details of the frequency and purpose of using music, who determined which music to use, and any effects, positive or negative, the interviewee believed to be associated with the use of music. Musical events, visiting musicians or music therapists leading group sing-alongs were observed at two communities, and music-related activities led by staff were observed at two others. Results/Anticipated Results: Music was used in some way at each of the five communities. Each location had recorded music available to residents in the shared living spaces, and most had a piano in the main lounge area. During the sing-along and music-related activities, residents were observed singing along to songs from memory, engaging with one another and the group leader and smiling. Staff employed by each community varied in their level of musical training and experience, from none to a full-time music therapist in residence. Staff interviewed said they believed music was helpful to aid memory recall, reduce anxiety, and to engage interest. Interestingly, a music therapist at one site also described how music during mealtimes created too much of a distraction for residents and interfered with dietary care. Discussion/Significance of Impact: It is clear from both the staff interviews and direct observations of musical activities that music is important to consider for people living with dementia in care communities. Guidelines for implementation and minimum standards would be helpful to ensure all care community residents can experience benefits highlighted by staff in this study.

The trace element selenium is known to protect against oxidative damage which is known to contribute to cognitive impairment with ageing (1,2). The aim of this study was to explore the association between selenium status (serum selenium and selenoprotein P (SELENOP)) and global cognitive performance at baseline and after 5 years in 85-year-olds living in the Northeast of England.

Serum selenium and SELENOP concentrations were measured at baseline by total reflection X-ray fluorescence (TXRF) and enzyme-linked immunosorbent assay (ELISA), respectively, in 757 participants from the Newcastle 85+ study. Global cognitive performance was assessed using the Standardized Mini-Mental State Examination (SMMSE) where scores ≤25 out of 30 indicated cognitive impairment. Logistic regressions explored the associations between selenium status and global cognition at baseline. Linear mixed models explored associations between selenium status and global cognition prospectively after 5 years. Covariates included sex, body mass index, physical activity, high sensitivity C-reactive protein, alcohol intake, self-rated health, medications and smoking status.

At baseline, in fully adjusted models, there was no increase in odds of cognitive impairment with serum selenium (OR 1.004, 95% CI 0.993-1.015, p = 0.512) or between SELENOP (OR 1.006, 95% CI 0.881-1.149, p = 0.930). Likewise, over 5 years, in fully adjusted models there was no association between serum selenium and cognitive impairment (β 7.20E-4 ± 5.57E-4, p = 0.197), or between SELENOP and cognitive impairment (β 3.50E-3 ± 6.85E-3, p = 0.610).

In this UK cohort of very old adults, serum selenium or SELENOP was not associated with cognitive impairment at baseline and 5 years. This was an unexpected finding despite SELENOP’s key role in the brain and the observed associations in other studies. Further research is needed to explore the effect of selenium on global cognition in very old adults.

This article used text mining processes to map continuity and change in policy principles of the National Disability Insurance Scheme (NDIS) in Australia and reflect on the underlying agendas of reform. Specifically, this research aimed to: (1) examine the substantive content of the NDIS legislative, operational and reform documentation, (2) assess changes in objects, principles and issues over time, and (3) discuss the implications of these shifting logics and agendas. To achieve this, text mining approaches were applied to ten key NDIS documents from 2011 to 2019.

The findings included a low prevalence of ‘rights’-based terminology, sustained attention to Scheme ‘costs’ but limited latterly attention to ‘sustainability’, and increasing prominence of interpersonal (e.g., ‘family’, ‘community’) and decision-making terminology (e.g., ‘decision’, ‘review’). How these shifts have influenced subsequent proposed reforms is explored and ongoing dilemmas about designing policy that ensures rights and entitlements, while balancing cost, sustainability and consistency are identified.

Over the last decade, substantial progress has been made in understanding the topology of quasi-two-dimensional (2-D) non-equilibrium fluid flows driven by ATP-powered microtubules and microorganisms. By contrast, the topology of three-dimensional (3-D) active fluid flows still poses interesting open questions. Here, we study the topology of a spherically confined active flow using 3-D direct numerical simulations of generalized Navier–Stokes (GNS) equations at the scale of typical microfluidic experiments. Consistent with earlier results for unbounded periodic domains, our simulations confirm the formation of Beltrami-like bulk flows with spontaneously broken chiral symmetry in this model. Furthermore, by leveraging fast methods to compute linking numbers, we explicitly connect this chiral symmetry breaking to the entanglement statistics of vortex lines. We observe that the mean of linking number distribution converges to the global helicity, consistent with the asymptotic result by Arnold [In Vladimir I. Arnold – Collected Works (ed. A.B. Givental, B.A. Khesin, A.N. Varchenko, V.A. Vassiliev & O.Y. Viro), pp. 357–375. Springer]. Additionally, we characterize the rate of convergence of this measure with respect to the number and length of observed vortex lines, and examine higher moments of the distribution. We find that the full distribution is well described by a k-Gamma distribution, in agreement with an entropic argument. Beyond active suspensions, the tools for the topological characterization of 3-D vector fields developed here are applicable to any solenoidal field whose curl is tangent to or cancels at the boundaries of a simply connected domain.

The behavior of mineral mixtures can be significantly different from the behavior of the individual components of the mixture due to differences between the mechanical and chemical properties of the individual minerals, and their ensuing effects on interparticle interactions and fabric formation. This study examines mixtures of kaolinite and calcium carbonate at different mass fractions using sedimentation, viscosity, and liquid-limit tests. These macroscale tests represent a wide range of solid-volume fractions and strain levels, with emphasis on high water-content conditions to magnify the effects of electrical forces. The results demonstrate that interparticle interactions depend on mineral surface-fluid effects, particle geometry, relative particle size, and solids content. With small solids contents, the kaolinite/calcium carbonate mixture behavior is a function of electrostatic interactions between oppositely charged mineral particles that promote flocculation; however, with large solids contents, the specific surface area of the minerals is the controlling factor. These results are relevant to many natural soil environments and to the possible development of engineered mineral mixtures for industrial applications.

Background: 5-Aminolevulinic acid (5-ALA) is a prodrug used to selectively illuminate high-grade glioma (HGG) tissue intra-operatively, shown to nearly double complete resection rates in a 2006 multicentre, phase III clinical trial. Here, we review the history of the 2020 approval of 5-ALA in Canada and present some of the first preliminary results on resection rates, survival analysis, and adverse effects from a single Canadian center. Methods: We enrolled 76 patients (median age 61 years, 42 male) with suspected HGG amenable to surgical resection between June 2020 and January 2023. Gross total resection was defined by the absence of enhancing lesions on postoperative MRI. We compared the survival distributions of confirmed HGG cases with complete vs. incomplete resection using a log-rank test and Kaplan-Meier statistic. Results: 52 patients were confirmed as having a HGG based on a pathological diagnosis. In 32 of these patients (60.3%) a gross total resection was achieved. 82.76% were still alive at 180 and 270 days, and 72.73% at 360 days. 47.8% had a survival of 600 or more days. Conclusions: 5-ALA fluorescence-guided surgery resulted in high complete resection rates, and improved overall survival comparable to the literature with no notable adverse side effects.

OBJECTIVES/GOALS: The trabecular meshwork (TM) and Schlemm’s canal (SC), located within the iridocorneal angle (ICA), form the main outflow pathway and a major target for glaucoma treatments. We characterized the human ICA in vivo with Optical Coherence Tomography (OCT) imaging using a customized goniolens and a commercial OCT device (Heidelberg Spectralis). METHODS/STUDY POPULATION: Imaging these structures is difficult due to the optical limitations of imaging through the cornea at high angles. Therefore, a clinical gonioscopy lens was modified with a 12mm plano-convex lens placed on its anterior surface to focus light on the ICA structures, and capture returning light. Each subjects’ eye was anesthetized with 1 drop of Proparacaine 0.5%. The goniolens was coupled to the eye with gonio-gel and it was held by a 3D adjustable mount. OCT volume scans were acquired on 10 healthy subjects. The linear polarization of the OCT was rotated with a half-waveplate to measure dependence of the ICA landmarks on polarization orientation. RESULTS/ANTICIPATED RESULTS: The TM was seen in 9 of 10 subjects. Polarization rotation modified the brightness of the band of extracanalicular limbal lamina (BELL) and corneoscleral bands due to the birefringent nature of the collagenous structures, increasing the contrast of SC. SC width was 99 ± 20µm varying in size over space, including a subject with SC narrowing in the inferior-temporal quadrant. DISCUSSION/SIGNIFICANCE: This clinically suitable gonioscopic OCT approach has successfully been used to image the human ICA in 3D in vivo, providing detailed characterization of the TM and SC as well as enhancing their contrast against their birefringent backgrounds by rotating the polarization of the imaging beam.

Much environmental enrichment for laboratory animals is intended to enhance animal welfare and normalcy by providing stimulation to reduce ‘boredom’. Behavioural manifestations of boredom include restless sensation-seeking behaviours combined with indicators of sub-optimal arousal. Here, we explored whether these signs could be reduced by extra daily play opportunity in laboratory ferrets (Mustela putorius furo). Specifically, we hypothesised that playtime would reduce restlessness, aggression, sensation-seeking and awake drowsiness, even 24 h later in the home-cage. Female ferrets (n = 14) were group-housed in enriched multi-level cages. Playtime involved exploring a room containing a ball pool, paper bags, balls containing bells, and a familiar interactive human for 1 h. This was repeated on three consecutive mornings, and on the fourth, home-cage behaviour was compared between ferrets which had experienced the playtime treatment versus control cage-mates which had not. Their investigation of stimuli (positive = mouse odour or ball; ambiguous = empty bottle or tea-strainer; and negative = peppermint or bitter apple odour) was also recorded. We then swapped treatments, creating a paired experimental design. Ferrets under control conditions lay awake with their eyes open and screeched significantly more, but slept and sat/stood less, than following playtime. They also contacted negative and ambiguous stimuli for significantly longer under control conditions than following playtime; contact with positive stimuli showed no effects. Attempts to blind the observer to treatments were unsuccessful, so replication is required, but the findings suggest that playtime may have reduced both sub-optimal arousal and restless sensation-seeking behaviour, consistent with reducing boredom.

We consider direct statistical simulation (DSS) of a paradigm system of convection interacting with mean flows. In the Busse annulus model, zonal jets are generated through the interaction of convectively driven turbulence and rotation; non-trivial dynamics including the emergence of multiple jets and bursting ‘predator–prey’ type dynamics can be found. We formulate the DSS by expanding around the mean flow in terms of equal-time cumulants and arrive at a closed set of equations of motion for the cumulants. Here, we present results using an expansion terminated at the second cumulant (CE2); it is fundamentally a quasilinear theory. We focus on particular cases including bursting and bistable multiple jets and demonstrate that CE2 can reproduce the results of direct numerical simulation if particular attention is given to symmetry considerations.

We numerically and theoretically investigate the Boussinesq Eady model, where a rapidly rotating density-stratified layer of fluid is subject to a meridional temperature gradient in thermal wind balance with a uniform vertically sheared zonal flow. Through a suite of numerical simulations, we show that the transport properties of the resulting turbulent flow are governed by quasigeostrophic (QG) dynamics in the rapidly rotating strongly stratified regime. The ‘vortex gas’ scaling predictions put forward in the context of the two-layer QG model carry over to this fully three-dimensional system: the functional dependence of the meridional flux on the control parameters is the same, the two adjustable parameters entering the theory taking slightly different values. In line with the QG prediction, the meridional heat flux is depth-independent. The vertical heat flux is such that turbulence transports buoyancy along isopycnals, except in narrow layers near the top and bottom boundaries, the thickness of which decreases as the diffusivities go to zero. The emergent (re)stratification is set by a simple balance between the vertical heat flux and diffusion along the vertical direction. Overall, this study demonstrates how the vortex-gas scaling theory can be adapted to quantitatively predict the magnitude and vertical structure of the meridional and vertical heat fluxes, and of the emergent stratification, without additional fitting parameters.