Cereal rye (Secale cereale L.) cover crop and preemergence herbicides are important components of an integrated weed management program for waterhemp [Amaranthus tuberculatus (Moq.) Sauer] and Palmer amaranth (Amaranthus palmeri S. Watson) management in soybean [Glycine max (L.) Merr.]. Accumulating adequate cereal rye biomass for effective suppression of Amaranthus spp. can be challenging in the upper Midwest due to the short window for cereal rye growth in a corn–soybean rotation. Farmers are adopting the planting green system to optimize cereal rye biomass production and weed suppression. This study aimed to evaluate the feasibility of planting soybean green when integrated with preemergence herbicides for the control of Amaranthus spp. under two soybean planting time frames. The study was conducted across 19 site-years in the United States over the 2021 and 2022 growing seasons. Factors included cover crop management practices (“no-till,” “cereal rye early-term,” and “cereal rye plant-green”), soybean planting times (“early” and “late”), and use of preemergence herbicides (“NO PRE” and “YES PRE”). Planting soybean green increased cereal rye biomass production by 33% compared with early termination. Greater cereal rye biomass production when planting green provided a 44% reduction in Amaranthus spp. density compared with no-till. The use of preemergence herbicides also resulted in a 68% reduction in Amaranthus spp. density compared with NO PRE. Greater cereal rye biomass produced when planting green reduced soybean stand, which directly reduced soybean yield in some site-years. Planting soybean green is a feasible management practice to optimize cereal rye biomass production, which, combined with preemergence herbicides, provided effective Amaranthus spp. management. Soybean stand was a key factor in maintaining soybean yields compared with no-till when planting green. Farmers should follow best management recommendations for proper planter and equipment setup to ensure effective soybean establishment under high levels of cereal rye biomass when planting green.

OBJECTIVES/GOALS: Glioblastomas (GBMs) are heterogeneous, treatment-resistant tumors that are driven by populations of cancer stem cells (CSCs). In this study, we perform an epigenetic-focused functional genomics screen in GBM organoids and identify WDR5 as an essential epigenetic regulator in the SOX2-enriched, therapy resistant cancer stem cell niche. METHODS/STUDY POPULATION: Despite their importance for tumor growth, few molecular mechanisms critical for CSC population maintenance have been exploited for therapeutic development. We developed a spatially resolved loss-of-function screen in GBM patient-derived organoids to identify essential epigenetic regulators in the SOX2-enriched, therapy resistant niche. Our niche-specific screens identified WDR5, an H3K4 histone methyltransferase responsible for activating specific gene expression, as indispensable for GBM CSC growth and survival. RESULTS/ANTICIPATED RESULTS: In GBM CSC models, WDR5 inhibitors blocked WRAD complex assembly and reduced H3K4 trimethylation and expression of genes involved in CSC-relevant oncogenic pathways. H3K4me3 peaks lost with WDR5 inhibitor treatment occurred disproportionally on POU transcription factor motifs, required for stem cell maintenance and including the POU5F1(OCT4)::SOX2 motif. We incorporated a SOX2/OCT4 motif driven GFP reporter system into our CSC cell models and found that WDR5 inhibitor treatment resulted in dose-dependent silencing of stem cell reporter activity. Further, WDR5 inhibitor treatment altered the stem cell state, disrupting CSC in vitro growth and self-renewal as well as in vivo tumor growth. DISCUSSION/SIGNIFICANCE: Our results unveiled the role of WDR5 in maintaining the CSC state in GBM and provide a rationale for therapeutic development of WDR5 inhibitors for GBM and other advanced cancers. This conceptual and experimental framework can be applied to many cancers, and can unmask unique microenvironmental biology and rationally designed combination therapies.

Work on procedural utility suggests that decision makers derive more value from outcomes earned with freedom of choice. We experimentally tested tradeoffs between procedural and outcome utility, examining financial losses as an important boundary condition. Participants completed a simulated consumer sales task (Exp. 1) or card task (Exp. 2) with or without choice. Participants reported their satisfaction with monetary outcomes. When given choice, participants reported greater self-determination. Participants also reported higher outcome satisfaction, but only for gains. Choice did not influence satisfaction for losses. In Experiment 2, Participants also preferred choice when selecting between financial gains. However, when choice was costly (large disparity in pay) or posed losses, most participants sacrificed choice for better payoffs. Results are consistent with a cognitive model in which participants shift their attention from procedural utilities to financial outcomes when faced with losses. Financial outcomes may take precedence over choice when financial outcomes are threatened.

The history of judgment and decision making is defined by a trend toward increasingly nuanced explanations of the decision making process. Recently, process models have become incredibly sophisticated, yet the tools available to directly test these models have not kept pace. These increasingly complex process models require increasingly complex process data by which they can be adequately tested. We propose a new class of data collection that will facilitate evaluation of sophisticated process models. Tracking mouse paths during a continuous response provides an implicit measure of the growth of preference that produces a choice—rather than the current practice of recording just the button press that indicates that choice itself. Recent research in cognitive science (Spivey & Dale, 2006) has shown that cognitive processing can be revealed in these dynamic motor responses. Unlike current process methodologies, these response dynamics studies can demonstrate continuous competition between choice options and even online preference reversals. Here, in order to demonstrate the mechanics and utility of the methodology, we present an example response dynamics experiment utilizing a common multi-alternative decision task.

Currently, a disparity exists between the process-level models decision researchers use to describe and predict decision behavior and the methods implemented and metrics collected to test these models. The current work seeks to remedy this disparity by combining the advantages of work in decision research (mouse-tracing paradigms with contingent information display) and cognitive psychology (eye-tracking paradigms from reading and scene perception). In particular, we introduce a new decision moving-window paradigm that presents stimulus information contingent on eye fixations. We provide data from the first application of this method to risky decision making, and show how it compares to basic eye-tracking and mouse-tracing methods. We also enumerate the practical, theoretical, and analytic advantages this method offers above and beyond both mouse-tracing with occlusion and basic eye tracking of information without occlusion. We include the use of new metrics that offer more precision than those typically calculated on mouse-tracing data as well as those not possible or feasible within the mouse-tracing paradigm.

Studies were conducted in 1988, 1989, and 1992 in Plains, GA to measure effects of paraquat and alachlor on ‘Florunner’ peanut. Peanut treated with paraquat (0.14 kg ai/ha) plus alachlor (3.4 kg ai/ha) applied at vegetative emergence (VE), or paraquat plus alachlor VE followed by paraquat 28 days after emergence (DAE) were compared with a nontreated control. Both herbicide treatments reduced peanut foliage biomass at 65 DAE in 1989 and 1992. Herbicide treatments did not affect foliage biomass 90 DAE in 1988 and 122 DAE in 1989, but paraquat plus alachlor followed by paraquat reduced foliage biomass at 122 DAE in 1992. Pod biomass, measured at 90 and 65 DAE in 1988 and 1992, respectively, was reduced by herbicides. However, pod biomass did not differ among treatments 122 DAE in 1989 and 1992. Percent reflectance from the peanut canopy measured no effects from herbicides in 1988. However, in 1989 and 1992 herbicides applied sequentially reduced peanut canopy development. Peanut treated with a single herbicide and sequentially took longer to mature. Once optimum maturity was reached, peanut yields were not reduced.

Herbicide-resistant Amaranthus spp. continue to cause management difficulties in soybean. New soybean technologies under development, including resistance to various combinations of glyphosate, glufosinate, dicamba, 2,4-D, isoxaflutole, and mesotrione, will make possible the use of additional herbicide sites of action in soybean than is currently available. When this research was conducted, these soybean traits were still regulated and testing herbicide programs with the appropriate soybean genetics in a single experiment was not feasible. Therefore, the effectiveness of various herbicide programs (PRE herbicides followed by POST herbicides) was evaluated in bare-ground experiments on glyphosate-resistant Palmer amaranth and glyphosate-resistant waterhemp (both tall and common) at locations in Arkansas, Illinois, Indiana, Missouri, Nebraska, and Tennessee. Twenty-five herbicide programs were evaluated; 5 of which were PRE herbicides only, 10 were PRE herbicides followed by POST herbicides 3 to 4 wks after (WA) the PRE application (EPOST), and 10 were PRE herbicides followed by POST herbicides 6 to 7 WA the PRE application (LPOST). Programs with EPOST herbicides provided 94% or greater control of Palmer amaranth and waterhemp at 3 to 4 WA the EPOST. Overall, programs with LPOST herbicides resulted in a period of weed emergence in which weeds would typically compete with a crop. Weeds were not completely controlled with the LPOST herbicides because weed sizes were larger (≥ 15 cm) compared with their sizes at the EPOST application (≤ 7 cm). Most programs with LPOST herbicides provided 80 to 95% control at 3 to 4 WA applied LPOST. Based on an orthogonal contrast, using a synthetic-auxin herbicide LPOST improves control of Palmer amaranth and waterhemp over programs not containing a synthetic-auxin LPOST. These results show herbicides that can be used in soybean and that contain auxinic- or HPPD-resistant traits will provide growers with an opportunity for better control of glyphosate-resistant Palmer amaranth and waterhemp over a wide range of geographies and environments.

Waterhemp has emerged as one of the most problematic weeds in agronomic crops in the Midwest because of an extended germination period and widespread occurrence of biotypes resistant to atrazine and sulfonylurea herbicides. However, the competitive effects of late-emerging cohorts on corn yield are not known. Field studies were conducted in 2001 and 2002 at Columbia, Novelty, and Albany, MO, to determine the effects of late-emerging waterhemp interference on corn growth, nitrogen (N) accumulation, and yield. Waterhemp emerged approximately 20 d after planting (DAP) and was treated at heights of 8, 15, 23, 31, 38, or 46 cm with directed applications of dicamba + diflufenzopyr followed by hand hoeing. Soil water status, corn leaf chlorophyll content, and corn and common waterhemp height were recorded at the time of waterhemp removal. N stress was detected with a chlorophyll meter at four of six removal timings at high waterhemp densities (362 or more plants/m2) but only at one of six removal timings at lower densities (82 or less plants/m2). Water stress was observed at five of the six removal timings at high densities but at none of the removal timings at low densities. High waterhemp densities reduced corn yield when allowed to reach 15 cm before removal, and yields were reduced 36% when not controlled. At low densities, yield losses did not occur unless waterhemp was allowed to remain with corn season long. Our research suggests that waterhemp is less competitive with corn than redroot pigweed, smooth pigweed, and Palmer amaranth. In addition, low densities of late-emerging waterhemp would not warrant removal to protect corn yield.

Fall-applied residual and spring preplant burn-down herbicide applications are typically used to control winter annual weeds and may also provide early-season residual control of summer annual weed species such as giant ragweed. Field experiments were conducted from 2006 to 2008 in southern Illinois to (1) assess the emergence pattern of giant ragweed, (2) evaluate the efficacy of several herbicides commonly used for soil-residual control of giant ragweed, and (3) investigate the optimal application timing of soil-residual herbicides for control of giant ragweed. Six herbicide treatments were applied at four application timings: early fall, late fall, early spring, and late spring. Giant ragweed first emerged in mid- and late-March in 2007 and 2008, respectively. The duration of emergence varied by year, with 95% of emergence complete in late May of 2008, but not until early July in 2007. Giant ragweed emergence occurred more quickly in plots that received a fall application of glyphosate + 2,4-D compared with the nontreated. Fall-applied residual herbicides did not reduce giant ragweed emergence in 2007 when compared with the nontreated, with the exception of chlorimuron + tribenuron applied in late fall. Giant ragweed control from early- and late-spring herbicide applications was variable by year. In 2007, saflufenacil (50 and 100 g ai ha−1) and simazine applied in early spring reduced giant ragweed densities by 95% or greater through mid-May; however, in 2008, early-spring applications failed to reduce giant ragweed emergence in mid-April. The only treatments that reduced giant ragweed densities by > 80% through early July were late-spring applications of chlorimuron + tribenuron or saflufenacil at 100 g ha−1. Thus, the emergence patterns of giant ragweed in southern Illinois dictates that best management with herbicides would include late-spring applications of soil-residual herbicides just before crop planting and most likely requires subsequent control with foliar or soil-residual herbicides after crop emergence.

Goss's bacterial wilt and leaf blight of corn is caused by the bacterium Clavibacter michiganensis subsp. nebraskensis (Cmn). This disease has recently re-emerged as an important disease in the Midwestern United States (US) and continues to spread. Cultural practices are currently the only methods available for controlling the disease. Weedy species in the genera Echinochloa, Setaria, and Sorghum have previously been described as alternative hosts of Cmn. The objective of this research was to use an isolate of Cmn from the eastern Midwest to examine the host status of previously confirmed hosts, as well as test whether additional weedy or cover crop species are alternative hosts of the bacterium. Plants were inoculated with a suspension of 1 × 108 colony-forming units of Cmn per milliliter in a greenhouse experiment. Leaves were observed for typical symptoms of Goss's wilt 7 d after inoculation. Pathogen presence was determined by observing bacterial streaming microscopically, and isolating Cmn from symptomatic plants. Putative colonies of Cmn were confirmed with the use of morphological and molecular methods. Koch's Postulates were completed on populations of new plant species that showed symptoms. Results revealed three new hosts of Cmn: annual ryegrass, johnsongrass, and large crabgrass. In contradiction to previous reports, barnyardgrass was not a host of Cmn in this study. Results also confirm that giant foxtail, green foxtail, shattercane, and yellow foxtail are hosts of Cmn. These results redefine the known host range of Cmn and are important in identifying additional sources of inoculum to improve our understanding of the epidemiology of Goss's wilt.

In North America, terrestrial records of biodiversity and climate change that span Marine Oxygen Isotope Stage (MIS) 5 are rare. Where found, they provide insight into how the coupling of the ocean–atmosphere system is manifested in biotic and environmental records and how the biosphere responds to climate change. In 2010–2011, construction at Ziegler Reservoir near Snowmass Village, Colorado (USA) revealed a nearly continuous, lacustrine/wetland sedimentary sequence that preserved evidence of past plant communities between ~140 and 55 ka, including all of MIS 5. At an elevation of 2705 m, the Ziegler Reservoir fossil site also contained thousands of well-preserved bones of late Pleistocene megafauna, including mastodons, mammoths, ground sloths, horses, camels, deer, bison, black bear, coyotes, and bighorn sheep. In addition, the site contained more than 26,000 bones from at least 30 species of small animals including salamanders, otters, muskrats, minks, rabbits, beavers, frogs, lizards, snakes, fish, and birds. The combination of macro- and micro-vertebrates, invertebrates, terrestrial and aquatic plant macrofossils, a detailed pollen record, and a robust, directly dated stratigraphic framework shows that high-elevation ecosystems in the Rocky Mountains of Colorado are climatically sensitive and varied dramatically throughout MIS 5.

Field experiments were conducted in 1999 and 2000 to determine the influence of mesotrione postemergence application rate, application timing, and addition of atrazine on corn injury, weed control, and corn grain yield. Corn injury in the form of leaf bleaching ranged from 0 to 15% at 7 d after treatment (DAT). In general, most of the bleaching injury rapidly dissipated with slight (≤ 8%) to no corn injury observed at 28 DAT. Control of common cocklebur with mesotrione at 14 DAT ranged from 79 to 98% for all treatments over both years. Applying mesotrione at 140 g/ha, at the early postemergence (EPOST) timing, or in combination with atrazine provided the greatest control of common cocklebur at 14 DAT. Application rate of mesotrione was the only factor that was significant in both years for control of common cocklebur later in the season at 56 DAT. Control of ivyleaf morningglory with mesostrione at 14 DAT ranged from 60 to 90% for all treatments in both years. Control of ivyleaf morningglory at 14 DAT was enhanced by the addition of atrazine to mesotrione. Control of ivyleaf morningglory at 56 DAT was greater with mid-postemergence and late postemergence than with EPOST applications, and was generally enhanced by the addition of atrazine. Yellow nutsedge control with mesotrione was inconsistent, ranging from 40 to 87% at 14 DAT for all treatments over both years. The addition of atrazine to mesotrione increased yellow nutsedge control from 47 to 87% at 14 DAT in 2000. Increasing the rate of mesotrione from 70 to 140 g/ha, as well as the addition of atrazine, improved control of yellow nutsedge at 56 DAT. Corn grain yield was not affected by corn injury or weed control as there were no significant differences in grain yield between herbicide-treated plots and handweeded plots.

Palmer amaranth and waterhemp have become increasingly troublesome weeds throughout the United States. Both species are highly adaptable and emerge continuously throughout the summer months, presenting the need for a residual PRE application in soybean. To improve season-long control of Amaranthus spp., 19 PRE treatments were evaluated on glyphosate-resistant Palmer amaranth in 2013 and 2014 at locations in Arkansas, Indiana, Nebraska, Illinois, and Tennessee; and on glyphosate-resistant waterhemp at locations in Illinois, Missouri, and Nebraska. The two Amaranthus species were analyzed separately; data for each species were pooled across site-years, and site-year was included as a random variable in the analyses. The dissipation of weed control throughout the course of the experiments was compared among treatments with the use of regression analysis where percent weed control was described as a function of time (the number of weeks after treatment [WAT]). At the mean (i.e., average) WAT (4.3 and 3.2 WAT for Palmer amaranth and waterhemp, respectively) isoxaflutole + S-metolachlor + metribuzin had the highest predicted control of Palmer amaranth (98%) and waterhemp (99%). Isoxaflutole + S-metolachlor + metribuzin, S-metolachlor + mesotrione, and flumioxazin + pyroxasulfone had a predicted control ≥ 97% and similar model parameter estimates, indicating control declined at similar rates for these treatments. Dicamba and 2,4-D provided some, short-lived residual control of Amaranthus spp. When dicamba was added to metribuzin or S-metolachlor, control increased compared to dicamba alone. Flumioxazin + pyroxasulfone, a currently labeled PRE, performed similarly to treatments containing isoxaflutole or mesotrione. Additional sites of action will provide soybean growers more opportunities to control these weeds and reduce the potential for herbicide resistance.

Waterhemp is a weed indigenous to the midwestern United States and is problematic in agronomic crop production. This weed is well suited to inhabit minimally tilled environments and is increasing in prevalence across many agricultural production areas and systems. A common garden experiment was established in Indiana in 2014 and 2015 with waterhemp populations from Indiana, Illinois, Missouri, Iowa, and Nebraska to compare the growth and development of waterhemp from these regions. Three establishment dates (May, June, and July) were used each year to simulate discontinuous germination. Mean biomass accumulations from the May (1,120 g plant−1) and June (1,069 g plant−1) establishment dates were higher than from the July (266 g plant−1) establishment date. There were no differences in biomass accumulations between the five populations in the May and June establishments, but biomass accumulations ranged from 195 to 338 g plant−1 in the July establishment. Mean seed yields were higher from the May (926,629 seeds plant−1) and June (828,905 seeds plant−1) establishment dates compared with the July (276,258 seeds plant−1) establishment. In the May and June establishments, seed yields ranged from 469,939 seeds plant−1 to 1,285,556 seeds plant−1. The Illinois population flowered the latest of all the populations yet also grew the tallest. The July establishment flowered the most rapidly after establishment, accumulated less biomass, and also had the largest seeds. This study demonstrated differences among waterhemp populations when grown in a common environment and the effect of establishment timing on waterhemp growth and development.