This study elucidated the impacts of coenzyme Q10 (COQ10) supplementation in a high-fat diet on growth, lipid metabolism, and mitochondrial function in spotted seabass (Lateolabrax maculatus). Totally five diets were formulated: a diet with normal fat content (11% lipid, NFD), a high-fat diet (17% lipid, HFD), and three additional diets by supplementing 5, 20 or 80 mg/kg of COQ10 to the HFD. After an 8-week culture period, samples were collected and analyzed. The results demonstrated that COQ10 inclusion prevented the HFD-induced deterioration of growth performance and feed utilization. COQ10 alleviated the deposition of saturated fatty acids following HFD intake and promoted the assimilation of n-3 and n-6 polyunsaturated fatty acids. Moreover, COQ10 administration inhibited the surge in serum transaminase activity and reduced hepatic lipid content following HFD ingestion, which was consistent with the results of oil red O staining. In addition, HFD feeding led to reduced hepatic citrate synthase and succinate dehydrogenase activities, and decreased ATP content. Notably, COQ10 administration improved these indices, and up-regulated the expression of mitochondrial biogenesis-related genes (pgc-1α, pgc-1β, nrf-1, tfam) and autophagy-related genes (pink1, mul1, atg5). In summary, supplementing 20-80 mg/kg of COQ10 in the HFD promoted growth performance, alleviated hepatic fat accumulation, and enhanced liver mitochondrial function in spotted seabass.

This study investigates the effects of fat emulsion-based early parenteral nutrition in patients following hemihepatectomy, addressing a critical gap in clinical knowledge regarding parenteral nutrition after hemihepatectomy. We retrospectively analysed clinical data from 274 patients who received non-fat emulsion-based parenteral nutrition (non-fatty nutrition group) and 297 patients who received fat emulsion-based parenteral nutrition (fatty nutrition group) after hemihepatectomy. Fat emulsion-based early parenteral nutrition significantly reduced levels of post-operative aspartate aminotransferase, total bilirubin and direct bilirubin, while minor decreases in red blood cell and platelet counts were observed in the fatty nutrition group. Importantly, fat emulsion-based early parenteral nutrition shortened lengths of post-operative hospital stay and fasting duration, but did not affect the incidence of short-term post-operative complications. Subgroup analyses revealed that the supplement of n-3 fish oil emulsions was significantly associated with a reduced inflammatory response and risk of post-operative infections. These findings indicate that fat emulsion-based early parenteral nutrition enhances short-term post-operative recovery in patients undergoing hemihepatectomy.

This study investigates the spatial distribution of inertial particles in turbulent Taylor–Couette flow. Direct numerical simulations are performed using a one-way coupled Eulerian–Lagrangian approach, with a fixed inner-wall Reynolds number of 2500 for the carrier flow, while the particle Stokes number ($St$) varies from 0.034 to 1 for the dispersed phase. We first examine the issue of preferential concentration of particles near the outer-wall region. Employing two-dimensional Voronoï analysis, we observe a pronounced particle clustering with increasing $St$, particularly evident in regions of low fluid velocity. Additionally, we investigate the concentration balance equation, inspired by the work of Johnson et al. (J. Fluid Mech., vol. 883, 2020, A27), to examine the particle radial distribution. We discern the predominant sources of influence, namely biased sampling, turbophoresis and centrifugal effects. Across all cases, centrifugal force emerges as the primary driver, causing particle migration toward the outer wall. Biased sampling predominantly affects smaller inertial particles, driving them toward the inner wall due to sampling within Taylor rolls with inward radial velocity. Conversely, turbophoresis primarily impacts larger inertial particles, inducing migration towards both walls where turbulent intensity is weaker compared with the bulk. With the revealed physics, our work provides a basis for predicting and controlling particle movement and distribution in industrial applications.

Based on a 4f system, a 0° reflector and a single laser diode side-pump amplifier, a new amplifier is designed to compensate the spherical aberration of the amplified laser generated by a single laser diode side-pump amplifier and enhance the power of the amplified laser. Furthermore, the role of the 4f system in the passive spherical aberration compensation and its effect on the amplified laser are discussed in detail. The results indicate that the amplification efficiency is enhanced by incorporating a 4f system in a double-pass amplifier and placing a 0° reflector only at the focal point of the single-pass amplified laser. This method also effectively uses the heat from the gain medium (neodymium-doped yttrium aluminium garnet) of the amplifier to compensate the spherical aberration of the amplified laser.

Vaccines have revolutionised the field of medicine, eradicating and controlling many diseases. Recent pandemic vaccine successes have highlighted the accelerated pace of vaccine development and deployment. Leveraging this momentum, attention has shifted to cancer vaccines and personalised cancer vaccines, aimed at targeting individual tumour-specific abnormalities. The UK, now regarded for its vaccine capabilities, is an ideal nation for pioneering cancer vaccine trials. This article convened experts to share insights and approaches to navigate the challenges of cancer vaccine development with personalised or precision cancer vaccines, as well as fixed vaccines. Emphasising partnership and proactive strategies, this article outlines the ambition to harness national and local system capabilities in the UK; to work in collaboration with potential pharmaceutic partners; and to seize the opportunity to deliver the pace for rapid advances in cancer vaccine technology.

Previous studies have found some puzzling power anomalies related to testing the indirect effect of a mediator. The power for the indirect effect stagnates and even declines as the size of the indirect effect increases. Furthermore, the power for the indirect effect can be much higher than the power for the total effect in a model where there is no direct effect and therefore the indirect effect is of the same magnitude as the total effect. In the presence of direct effect, the power for the indirect effect is often much higher than the power for the direct effect even when these two effects are of the same magnitude. In this study, the limiting distributions of related statistics and their non-centralities are derived. Computer simulations are conducted to demonstrate their validity. These theoretical results are used to explain the observed anomalies.

This research concerns a mediation model, where the mediator model is linear and the outcome model is also linear but with a treatment–mediator interaction term and a residual correlated with the residual of the mediator model. Assuming the treatment is randomly assigned, parameters in this mediation model are shown to be partially identifiable. Under the normality assumption on the residual of the mediator and the residual of the outcome, explicit full-information maximum likelihood estimates of model parameters are introduced given the correlation between the residual for the mediator and the residual for the outcome. A consistent variance matrix of these estimates is derived. Currently, the coefficients of this mediation model are estimated using the iterative feasible generalized least squares (IFGLS) method that is originally developed for seemingly unrelated regressions (SURs). We argue that this mediation model is not a system of SURs. While the IFGLS estimates are consistent, their variance matrix is not. Theoretical comparisons of the FIMLE variance matrix and the IFGLS variance matrix are conducted. Our results are demonstrated by simulation studies and an empirical study. The FIMLE method has been implemented in a freely available R package iMediate.

This study aims to investigate the effects of the vine of Lonicera japonica Thunb (VLT) and marine-derived Bacillus amyloliquefaciens-9 (BA-9) supplementation on the growth performance, antioxidant capacity, and gut microbiota of goat kids. A total of 32 4-week-old kids were randomly assigned into four groups: a control group (CON), a group supplemented with 0.3% BA-9 (BA-9), a group supplemented with 2% VLT (VLT), and a group supplemented with both 0.3% BA-9 and 2% VLT (MIX). The results indicated that VLT supplementation significantly increased both average daily (P < 0.001) and total weight gain (TWG) (P < 0.001), while BA-9 alone had no significant effect (P > 0.05) on the average daily and TWG. Biomarker analysis of oxidative stress revealed that supplementation of VLT or BA-9 alone enhanced antioxidant capacity. The MIX group showing a higher total antioxidant capacity (T-AOC) compared with the CON, VLT, and BA-9 groups (P < 0.05). Plasma albumin (ALB) levels were significantly increased in the both VLT and BA-9 groups. Microbiota analysis revealed significant differences in α-diversity and β-diversity between the MIX and CON groups, with specific genera such as Prevotellaceae_UCG.004 and Rikenellaceae_RC9_gut_group negatively correlated with average daily gain (ADG), while Alistipes was positively correlated with T-AOC. These findings suggest that the combined supplementation of VLT and BA-9 can significantly enhance growth performance and antioxidant capacity in goat kids by modulating the composition of gut microbiota and reducing oxidative stress.

We study the melting process of a solid under microgravity, driven solely by lateral vibrations that are perpendicular to the applied temperature gradient due to the absence of gravity-induced convection. Using direct numerical simulations with the phase-field method, we examine two-dimensional vibration-induced melting in a square cavity over four orders of magnitude of vibrational Rayleigh numbers, $10^5\le Ra_{{vib}}\le 10^9$. Our results show that as melting progresses, the flow structure transitions from a periodic-circulation regime with diffusion-dominated heat transfer to a columnar regime with vibroconvection. The mean height of the liquid–solid interface follows a power-law dependency with time, $\bar {\xi } \sim \tilde t^{1/(2-2\alpha )}$, where $\alpha = 0$ in the periodic-circulation regime and $\alpha = 1/2$ in the columnar regime. We further observe that within the columnar regime, the morphological evolution of the liquid–solid interface is influenced by the interaction of columnar thermal plumes in the central regions and the peripheral flow near the sidewalls. Specifically, we offer a comprehensive analysis of the plume merging behaviour, which is governed by the aspect ratio ($\bar {\xi }$) of the liquid layer and the intensity of vibration, quantified by the effective vibrational Rayleigh number $Ra_{vib}^{eff}$. We identify the relationship between the number of columnar plumes $K_m$ and $Ra_{vib}^{eff}$, finding that $K_m \sim \bar {\xi }^{-1} (Ra_{vib}^{eff})^{\gamma }$ with the fitting scaling exponent $\gamma = 0.150 \pm 0.025$. We subsequently quantify the characteristics of the interface roughness amplitude evolution in microgravity vibroconvection. Our results indicate that the roughness amplitude exhibits a power-law dependence on the mean height of the liquid layer. Drawing from the Stefan boundary condition, we theoretically deduce this dependence under the assumption of a non-uniform heat flux distribution at the interface, where the theory is corroborated by our numerical simulations.

Remote center-of-motion (RCM) manipulators are a key issue in minimally invasive surgeries (MIS). The existing RCM parallel mechanisms (PMs) can only generate RCM motion based on the invariant RCM. To provide mobility for RCM, this paper designed a new family of RCM PMs with movable RCM that features a double-stage topological structure. Drawing mainly on configuration evolution and Lie-group, a general approach is proposed to design double-stage PMs with movable RCM. Feasible limbs for 2R1T RCM motion are enumerated and used to construct the secondary PM. Type synthesis of the primary PMs that realize movable RCM is accomplished based on the method presented. Different connection styles between the two stages that ensure the geometrical conditions of RCM motion are designed. Using different connection styles, double-stage PMs with movable RCM are constructed. These new RCM PMs can realize precise positioning of RCM by taking advantage of the primary PMs, which indicates their potential application prospects in MIS.

Anomalous left coronary artery from the pulmonary artery is a rare CHD. It is the most common type of anomalous coronary origin. It may cause myocardial ischaemia or infarction, mitral regurgitation, congestive heart failure, and early death in infancy if left untreated. Surgery is the only treatment for anomalous left coronary artery from the pulmonary artery. In recent years, with advancements in surgical techniques and the widespread utilisation of extracorporeal cardiac assist devices such as extracorporeal membrane oxygenation, the treatment outcomes for anomalous left coronary artery from the pulmonary artery have demonstrated significant improvements. However, the surgical indications and methods of anomalous left coronary artery from the pulmonary artery, especially the surgical methods of anomalous left coronary artery from the pulmonary artery with intramural coronary artery, and whether to treat mitral regurgitation at the same time are still controversial. The long-term complications and prognosis remain discouraging simultaneously, with significant variations in outcomes across different centres. The present review specifically addresses these aforementioned concerns. Based on the literature published at home and abroad, we found that no matter what type of anomalous left coronary artery from the pulmonary artery patients, even asymptomatic patients, regardless of the collateral circulation between the left and right coronary arteries, should immediately undergo surgical treatment to promote the recovery of left ventricular function. Based on different coronary artery anatomical morphology and preoperative cardiac function, the long-term follow-up results of individualised surgical treatment of anomalous left coronary artery from the pulmonary artery children show good prognosis, and most children have significant improvement in cardiac function. Patients with moderate to severe mitral regurgitation should undergo mitral valve operation at the same time as anomalous left coronary artery from the pulmonary artery repair. Mitral valvuloplasty can quickly improve mitral regurgitation and promote the early recovery of cardiac function after operation, and does not increase the risk of operation. Mechanical circulatory support is a safe and effective means of early postoperative transition for children with severe anomalous left coronary artery from the pulmonary artery. Anomalous left coronary artery from the pulmonary artery with intramural coronary artery is a rare anomaly. According to different anatomical types, different surgical methods can be used for anatomical correction, and satisfactory early and mid-term results can be obtained.

Pulmonary valve replacement and right ventricular outflow tract reconstruction with valved conduits have been the shortcomings of paediatric cardiac surgeons in the treatment of CHD. In recent decades, encouraging achievements have been made in right ventricular outflow tract technology. Since Klinner reported the first right ventricle-to-pulmonary artery connection using unvalved conduits made of autologous pericardium in 1964, various right ventricle-to-pulmonary artery conduits have gradually been used in the treatment of various complex CHD. Compared with other materials, valved homograft conduit (VHC) is more consistent with physiological characteristics, better haemodynamics, easy suture and good haemostasis, anti-calcification, anti-infection, and without the need for lifelong anticoagulation, which makes VHC the best material for reconstruction of right ventricular outflow tract. However, due to the shortage of donor sources, other alternative conduits such as polytetrafuoroethylene valved conduits have been developed, and the results are not inferior to VHC in clinical application. The emerging tissue engineering technology is expected to utilise recipient-derived endothelial cells for implantation onto the decellularized VHC or degradable synthetic materials in order to construct a recipient-specific tissue-engineered valved conduit. This advancement holds great potential as an ideal biological transplant material and valve replacement for CHD. It will completely solve the problems of immune rejection and the growth of the conduit that cannot adapt to the physical growth of children. This review provides a comprehensive review of the clinical indications for right ventricle-to-pulmonary artery conduits application, optimal timing for surgery, current practices in utilising various types of external conduits, and considerations for re-replacement.

In the transitioning era towards the COVID-19 endemic, there is still a sizable population that has never been vaccinated against COVID-19 in the Netherlands. This study employs Bayesian spatio-temporal modelling to assess the relative chances of COVID-19 vaccination uptake – first, second, and booster doses – both at the municipal and regional (public health services) levels. Incorporating ecological regression modelling to consider socio-demographic factors, our study unveils a diverse spatio-temporal distribution of vaccination uptake. Notably, the areas located in or around the Dutch main urban area (Randstad) and regions that are more religiously conservative exhibit a below-average likelihood of vaccination. Analysis at the municipal level within public health service regions indicates internal heterogeneity. Additionally, areas with a higher proportion of non-Western migrants consistently show lower chances of vaccination across vaccination dose scenarios. These findings highlight the need for tailored national and local vaccination strategies. Particularly, more regional efforts are essential to address vaccination disparities, especially in regions with elevated proportions of marginalized populations. This insight informs ongoing COVID-19 campaigns, emphasizing the importance of targeted interventions for optimizing health outcomes during the second booster phase, especially in regions with a relatively higher proportion of marginalized populations.

Cryogenic carbon capture (CCC) is an innovative technology to desublimate $\text {CO}_2$ out of industrial flue gases. A comprehensive understanding of $\text {CO}_2$ desublimation and sublimation is essential for widespread application of CCC, which is highly challenging due to the complex physics behind. In this work, a lattice Boltzmann (LB) model is proposed to study $\text {CO}_2$ desublimation and sublimation for different operating conditions, including the bed temperature (subcooling degree $\Delta T_s$), gas feed rate (Péclet number $Pe $) and bed porosity ($\psi$). The $\text {CO}_2$ desublimation and sublimation properties are reproduced. Interactions between convective $\text {CO}_2$ supply and desublimation/sublimation intensity are analysed. In the single-grain case, $Pe $ is suggested to exceed a critical value $Pe _c$ at each $\Delta T_s$ to avoid the convection-limited regime. Beyond $Pe _c$, the $\text {CO}_2$ capture rate ($v_c$) grows monotonically with $\Delta T_s$, indicating a desublimation-limited regime. In the packed bed case, multiple grains render the convective $\text {CO}_2$ supply insufficient and make CCC operate under the convection-limited mechanism. Besides, in small-$\Delta T_s$ and high-$Pe $ tests, $\text {CO}_2$ desublimation becomes insufficient compared with convective $\text {CO}_2$ supply, thus introducing the desublimation-limited regime with severe $\text {CO}_2$ capture capacity loss ($\eta _d$). Moreover, large $\psi$ enhances gas mobility while decreasing cold grain volume. A moderate porosity $\psi _c$ is recommended for improving the $\text {CO}_2$ capture performance. By analysing $v_c$ and $\eta _d$, regime diagrams are proposed in $\Delta T_s$–$Pe $ space to show distributions of convection-limited and desublimation-limited regimes, thus suggesting optimal conditions for efficient $\text {CO}_2$ capture. This work develops a viable LB model to examine CCC under extensive operating conditions, contributing to facilitating its application.

Integrons are important genetic elements that allow easy acquisition and dissemination of antimicrobial resistance genes. Studies reporting occurrence of integrons in Staphylococcus aureus (S. aureus) isolated from bovine mastitis in large dairy farms across China are scarce. The aim of this study was to investigate the occurrence of class 1 integrons (intI1), antimicrobial resistance (AMR) and associated genes in S. aureus isolated from bovine mastitis and their associations. Minimum inhibitory concentrations (MICs) were determined to evaluate the AMR phenotypes, whereas PCR was carried out to assess the occurrence of AMR genes and intI1. In addition, index cluster analysis was used to estimate associations between AMR phenotype, genotype and intI1 in 103 isolates. Overall, 83% of S. aureus were intI1-positive and 5 types of gene cassettes were detected. Susceptibility against single antimicrobial agents ranged from 0% (erythromycin), 12% (ampicillin) and 16% (penicillin G) to 96% (gentamicin). Most isolates (64%) were intermediate-resistant against erythromycin, whereas resistance against ceftriaxone (22%), clindamycin (4%), cefotaxime (2%), tetracycline (1%) and ciprofloxacin (1%) were relatively uncommon. The predominant resistant gene was blaZ gene (n = 88, 85%) followed by tetD gene (n = 85, 83%). With an estimated prevalence of 12% of the mecA gene, methicillin-resistant S. aureus isolates had higher MIC50 and MIC90 for majority of antimicrobials than methicillin-susceptible S. aureus isolates. Presence of the ermC gene was associated with erythromycin resistance. Ampicillin, erythromycin and penicillin G resistance were associated with intI1. The data presented in our study indicated that class 1 integron-mediated resistance possibly plays an important role in dissemination of AMR in S. aureus isolated from bovine mastitis.

In 2017, Brosseau & Vlahovska (Phys. Rev. Lett, vol. 119, no. 3, 2017, p. 034501) found that, in a strong electric field, a weakly conductive, low-viscosity droplet immersed in a highly conductive, high-viscosity medium formed a lens shape, and liquid rings continuously detached from its equatorial plane and subsequently broke up into satellite droplets. This fascinating multiphase electrohydrodynamic (EHD) phenomenon is known as droplet equatorial streaming. In this paper, based on the unified lattice Boltzmann method framework proposed by Luo et al. (Phil. Trans. R. Soc. A Math. Phys. Engng Sci, vol. 379, no. 2208, 2021, p. 20200397), a novel lattice Boltzmann (LB) model is constructed for multiphase EHD by coupling the Allen–Cahn type of multiphase LB model and two new LB equations to solve the Poisson equation of the electric field and the conservation equation of the surface charge. Using the proposed LB model, we successfully reproduced, for the first time, the complete process of droplet equatorial streaming, including the continuous ejection and breakup of liquid rings on the equatorial plane. In addition, it is found that, under conditions of high electric field strength or significant electrical conductivity contrast, droplets exhibit fingering equatorial streaming that was unknown before. A power-law relationship is discovered for droplet total charge evolution and a theoretical model is then proposed to describe the droplet radius and height over time. The breakup of liquid rings is found to be dominated by capillary instability, while the breakup of liquid fingers is governed by the end-pinching mechanism. Finally, a phase diagram is constructed for fingering equatorial streaming and ring equatorial streaming, and a criterion equation is established for the phase boundary.

We report the unified constitutive law of vibroconvective turbulence in microgravity, i.e. $Nu \sim a^{-1} Re_{os}^\beta$ where the Nusselt number $Nu$ measures the global heat transport, $a$ is the dimensionless vibration amplitude, $Re_{os}$ is the oscillational Reynolds number and $\beta$ is the universal exponent. We find that the dynamics of boundary layers plays an essential role in vibroconvective heat transport and the $Nu$-scaling exponent $\beta$ is determined by the competition between the thermal boundary layer (TBL) and vibration-induced oscillating boundary layer (OBL). Then a physical model is proposed to explain the change of scaling exponent from $\beta =2$ in the TBL-dominant regime to $\beta = 4/3$ in the OBL-dominant regime. Our finding elucidates the emergence of universal constitutive laws in vibroconvective turbulence, and opens up a new avenue for generating a controllable effective heat transport under microgravity or even microfluidic environment in which the gravity effect is nearly absent.