North Carolina growers have long struggled to control Italian ryegrass, and recent research has confirmed that some Italian ryegrass biotypes have become resistant to nicosulfuron, glyphosate, clethodim, and paraquat. Integrating alternative management strategies is crucial to effectively control such biotypes. The objectives of this study were to evaluate Italian ryegrass control with cover crops and fall-applied residual herbicides and investigate cover crop injury from residual herbicides. This study was conducted during the fall/winter of 2021–22 in Salisbury, NC, and fall/winter of 2021–22 and 2022–23 in Clayton, NC. The study was designed as a 3 × 5 split-plot in which the main plot consisted of three cover crop treatments (no-cover, cereal rye at 80 kg ha−1, and crimson clover at 18 kg ha−1), and the subplots consisted of five residual herbicide treatments (S-metolachlor, flumioxazin, metribuzin, pyroxasulfone, and nontreated). In the 2021–22 season at Clayton, metribuzin injured cereal rye and crimson clover 65% and 55%, respectively. However, metribuzin injured both cover crops ≤6% in 2022–23. Flumioxazin resulted in unacceptable crimson clover injury of 50% and 38% in 2021–22 and 2022–23 in Clayton and 40% in Salisbury, respectively. Without preemergence herbicides, cereal rye controlled Italian ryegrass by 85% and 61% at 24 wk after planting in 2021–22 and 2022–23 in Clayton and 82% in Salisbury, respectively. In 2021–22, Italian ryegrass seed production was lowest in cereal rye plots at both locations, except when it was treated with metribuzin. For example, in Salisbury, cereal rye plus metribuzin resulted in 39,324 seeds m–2, compared to ≤4,386 seeds m–2 from all other cereal rye treatments. In 2022–23, Italian ryegrass seed production in cereal rye was lower when either metribuzin or pyroxasulfone were used preemergence (2,670 and 1,299 seeds m–2, respectively) compared with cereal rye that did not receive an herbicide treatment (5,600 seeds m–2). cereal rye (Secale cereale L.) and crimson clover (Trifolium incarnatum L.)

Policies that promote conversion of antibiotics from intravenous to oral route administration are considered “low hanging fruit” for hospital antimicrobial stewardship programs. We developed a simple metric based on digestive days of therapy divided by total days of therapy for targeted agents and a method for hospital comparisons. External comparisons may help identify opportunities for improving prospective implementation.

Cyclosporiasis is an illness characterised by watery diarrhoea caused by the food-borne parasite Cyclospora cayetanensis. The increase in annual US cyclosporiasis cases led public health agencies to develop genotyping tools that aid outbreak investigations. A team at the Centers for Disease Control and Prevention (CDC) developed a system based on deep amplicon sequencing and machine learning, for detecting genetically-related clusters of cyclosporiasis to aid epidemiologic investigations. An evaluation of this system during 2018 supported its robustness, indicating that it possessed sufficient utility to warrant further evaluation. However, the earliest version of CDC's system had some limitations from a bioinformatics standpoint. Namely, reliance on proprietary software, the inability to detect novel haplotypes and absence of a strategy to select an appropriate number of discrete genetic clusters would limit the system's future deployment potential. We recently introduced several improvements that address these limitations and the aim of this study was to reassess the system's performance to ensure that the changes introduced had no observable negative impacts. Comparison of epidemiologically-defined cyclosporiasis clusters from 2019 to analogous genetic clusters detected using CDC's improved system reaffirmed its excellent sensitivity (90%) and specificity (99%), and confirmed its high discriminatory power. This C. cayetanensis genotyping system is robust and with ongoing improvement will form the basis of a US-wide C. cayetanensis genotyping network for clinical specimens.

Despite the rapid growth of online political advertising, the vast majority of scholarship on political advertising relies exclusively on evidence from candidates’ television advertisements. The relatively low cost of creating and deploying online advertisements and the ability to target online advertisements more precisely may broaden the set of candidates who advertise and allow candidates to craft messages to more narrow audiences than on television. Drawing on data from the newly released Facebook Ad Library API and television data from the Wesleyan Media Project, we find that a much broader set of candidates advertises on Facebook than television, particularly in down-ballot races. We then examine within-candidate variation in the strategic use and content of advertising on television relative to Facebook for all federal, statewide, and state legislative candidates in the 2018 election. Among candidates who use both advertising media, Facebook advertising occurs earlier in the campaign, is less negative, less issue focused, and more partisan than television advertising.

We compared sepsis “time zero” and Centers for Medicare and Medicaid Services (CMS) SEP-1 pass rates among 3 abstractors in 3 hospitals. Abstractors agreed on time zero in 29 of 80 (36%) cases. Perceived pass rates ranged from 9 of 80 cases (11%) to 19 of 80 cases (23%). Variability in time zero and perceived pass rates limits the utility of SEP-1 for measuring quality.

Patient days and days present were compared to directly measured person time to quantify how choice of different denominator metrics may affect antimicrobial use rates. Overall, days present were approximately one-third higher than patient days. This difference varied among hospitals and units and was influenced by short length of stay.

Infect Control Hosp Epidemiol 2018;39:612–615

Greenhouse experiments in central Texas assessed the relative importance of above- and belowground interactions of semidwarf Mit wheat and Marshall ryegrass during vegetative growth. One experiment used partitions to compare the effect of no (controls), aboveground only, belowground only, and full interaction for 75 d after planting (DAP) one wheat and nine ryegrass plants in soil volumes of 90, 950, and 3,800 ml. The results with the different soil volumes were similar. Wheat growth in the aboveground interaction only did not differ from controls. However, the full or belowground only interaction of wheat with ryegrass reduced wheat height, leaf number, tillering, leaf area, percent total nonstructural carbohydrates in shoot, and dry weights of leaves, stems, and roots 45 and 75 DAP compared to controls. Wheat in full and belowground interaction only did not differ from one another in growth. A replacement series experiment of 56 d also showed that the competitive advantage of ryegrass was relatively greater in root than in shoot growth. No allelopathic response of wheat to ryegrass occurred. While the tallness of the semidwarf wheat minimized aboveground interference by ryegrass, the root growth of the thinner and more fibrous roots of ryegrass greatly enhanced its belowground competitiveness.

A greenhouse experiment compared the vegetative growth in pure cultures and mixtures of winter Triticum aestivum cultivar ‘Mit’ and Lolium multiflorum cultivar ‘Marshall’ in continuously watered controls and drought treatments. Control L. multiflorum in pure culture 14 wk after planting produced more leaf area, tillers, and dry weights of stem and root than control T. aestivum in pure culture. The greater seed size, larger initial leaf area, and height allowed T. aestivum to produce greater final leaf area and dry stem weight in control mixtures than L. multiflorum. Watering following drought shifted the relative performance of the two species in pure cultures and mixtures compared to controls. The ability of T. aestivum to maintain a greater leaf expansion rate during drought and a greater leaf area afterward than L, multiflorum allowed T. aestivum to attain greater growth than L. multiflorum in pure cultures exposed to temporary drought followed by watering. Conversely, drought and its relief enhanced the relative competitiveness of L. multiflorum compared to controls in mixtures with T. aestivum. During 4 wk of watering following the drought, L. multiflorum in mixtures grew vigorously and was similar to T. aestivum in all measures except in height and dry stem weight. Thus, L. multiflorum was similar in root growth with T. aestivum in control and drought mixtures and had its aboveground competitiveness amplified by the cycle of drought and watering in this study. There was no evidence of an allelopathic interaction between the two species.

Anomalous aortic origin of the coronary arteries is associated with exercise-induced ischaemia, leading some physicians to restrict exercise in patients with this condition. We sought to determine whether exercise restriction was associated with increasing body mass index over time. From 1998 to 2015, 440 patients ⩽30 years old were enrolled into an inception cohort. Exercise-restriction status was documented in 143 patients. Using linear mixed model repeated-measures regression, factors associated with increasing body mass index z-score over time, including exercise restriction and surgical intervention as time-varying covariates, were investigated. The 143 patients attended 558 clinic visits for which exercise-restriction status was recorded. The mean number of clinic visits per patient was 4, and the median duration of follow-up was 1.7 years (interquartile range (IQR) 0.5–4.4). The median age at first clinic visit was 10.3 years (IQR 7.1–13.9), and 71% (101/143) were males. All patients were alive at their most recent follow-up. At the first clinic visit, 54% (78/143) were exercise restricted, and restriction status changed in 34% (48/143) during follow-up. The median baseline body mass index z-score was 0.2 (IQR 0.3–0.9). In repeated-measures analysis, neither time-related exercise restriction nor its interaction with time was associated with increasing body mass index z-score. Surgical intervention and its interaction with time were associated with decreasing body mass index z-score. Although exercise restriction was not associated with increasing body mass index over time, surgical intervention was associated with decreasing body mass index z-score over time in patients with anomalous aortic origin of the coronary arteries.

Annual bluegrass is a pervasive weed on golf courses in the Transition Zone of the United States and is difficult to selectively remove. For years, superintendents have applied glyphosate on dormant zoysiagrass to remove cool-season weeds. In 2007, a population of annual bluegrass in Columbia, MO, was not controlled with glyphosate after more than 10 yr of continuous applications. Greenhouse studies were established to compare the response of suspect glyphosate-resistant (CCMO1) and -susceptible annual bluegrass to glyphosate. Seedling plants were treated with glyphosate from 0 to 6.27 kg ae ha−1. At 21 d after treatment, reductions in biomass for susceptible annual bluegrass reached a maximum at glyphosate rates of 0.78 kg ha−1 or higher. Comparatively, the biomass of CCMO1 plants was only reduced by 50% at 0.78 kg ha−1, and reductions did not exceed 60% at rates up to 6.27 kg ha−1, which is eight times the labeled rate. At rates necessary to reduce plant dry weights by 50%, the resistance factor (RF) for CCMO1 was 5.2. Twenty-one days following biomass assessment, regrowth of plants was non-existent on susceptible plants at 0.78 kg ha−1 glyphosate or above, but CCMO1 plants reached 1.7 cm regrowth at the 6.27 kg ha−1 rate. Based on the regrowth, the RF for CCMO1 was 5.2. Results indicate a new species has been identified with resistance to glyphosate, and this represents the first report of glyphosate resistance in turfgrass.

A greenhouse experiment used a replacement series design to compare the vegetative growth 6 wk after emergence in pure cultures and mixtures of winter wheat and Italian ryegrass, with phosphorus (P) levels recommended by soil testing. The planting proportions of wheat and Italian ryegrass were 100 and 0%, 75 and 25%, 50 and 50%, 25 and 75%, and 0 and 100%, respectively. There was no alleopathic interaction between the species. Both species in all pure and mixed cultures had substantially less growth in the low-P than in the recommended P treatment. However, the relative performance of the two species differed between P treatments. In the recommended P treatment in pure culture, Italian ryegrass had more tillers and greater root weight and length than wheat. Pure culture wheat in the low-P treatment exceeded pure culture Italian ryegrass in leaf area, weights of leaves, stems, and roots, and root length. Thus, the growth of wheat was inhibited less by P deficiency than the growth of Italian ryegrass in pure culture. In the 50:50 mixture of the recommended P treatment, wheat had greater leaf, stem, and root weights than Italian ryegrass. In the 50:50 mixture of the low-P treatment, the two species were very similar in growth, except that Italian ryegrass had about three times more tillers than did wheat. Whereas P deficiency limited the growth of wheat less than Italian ryegrass in pure culture, P deficiency did not affect the relative competitiveness of Italian ryegrass as much as wheat in mixed cultures. The ability of Italian ryegrass to compete with wheat when P was limiting may result from a difference in root growth. Italian ryegrass had a greater fresh root length to fresh root weight ratio than did wheat in the low-P treatment in pure culture and in the 50:50 mixture. The greater surface area of Italian ryegrass roots likely enhanced the competitiveness of Italian ryegrass relative to wheat under P-deficit conditions. Thus, the use of the recommended P nutrition from soil testing may be a key component to diminish Italian ryegrass competition in wheat fields.

Fluvial geomorphology and stratigraphy often reflect past environmental and climate conditions. This study examines the response of Bull Creek, a small ephemeral creek in the Oklahoma panhandle, to environmental conditions through the late Pleistocene and Holocene. Fluvial terraces were mapped and their stratigraphy and sedimentology documented throughout the course of the main valley. Based on their elevations, terraces were broadly grouped into a late-Pleistocene fill terrace (T3) and two Holocene fill-cut terrace sets (T2 and T1). Terrace systems are marked by similar stratigraphies recording the general environmental conditions of the time. Sedimentary sequences preserved in terrace fills record the transition from a perennial fluvial system during the late glacial period and the Younger Dryas to a semiarid environment dominated by loess accumulation and punctuated by flood events during the middle to late Holocene. The highest rates of aeolian accumulation within the valley occurred during the early to middle Holocene. Our data provide significant new information regarding the late-Pleistocene and Holocene environmental history for this region, located between the well-studied Southern and Central High Plains of North America.

High-temperature X-ray diffraction with concurrent gas chromatography (GC) was used to study cobalt disulfide cathode pellets disassembled from thermal batteries. When CoS2 cathode materials were analyzed in an air environment, oxidation of the K(Br, Cl) salt phase in the cathode led to the formation of K2SO4 that subsequently reacted with the pyrite-type CoS2 phase leading to cathode decomposition between ~260 and 450 °C. Independent thermal analysis experiments, i.e. simultaneous thermogravimetric analysis/differential scanning calorimetry/mass spectrometry (MS), augmented the diffraction results and support the overall picture of CoS2 decomposition. Both gas analysis measurements (i.e. GC and MS) from the independent experiments confirmed the formation of SO2 off-gas species during breakdown of the CoS2. In contrast, characterization of the same cathode material under inert conditions showed the presence of CoS2 throughout the entire temperature range of analysis.

Human illness attribution is recognized as an important metric for prioritizing and informing food-safety decisions and for monitoring progress towards long-term food-safety goals. Inferences regarding the proportion of illnesses attributed to a specific commodity class are often based on analyses of datasets describing the number of outbreaks in a given year or combination of years. In many countries, the total number of pathogen-related outbreaks reported nationwide for an implicated food source is often fewer than 50 instances in a given year and the number of years for which data are available can be fewer than 10. Therefore, a high degree of uncertainty is associated with the estimated fraction of pathogen-related outbreaks attributed to a general food commodity. Although it is possible to make inferences using only data from the most recent year, this type of estimation strategy ignores the data collected in previous years. Thus, a strong argument exists for an estimator that could ‘borrow strength’ from data collected in the previous years by combining the current data with the data from previous years. While many estimators exist for combining multiple years of data, most either require more data than is currently available or lack an objective and biologically plausible theoretical basis. This study introduces an estimation strategy that progressively reduces the influence of data collected in past years in accordance with the degree of departure from a Poisson process. The methodology is applied to the estimation of the attribution fraction for Salmonella and Escherichia coli O157:H7 for common food commodities and the estimates are compared against two alternative estimators.