The effect dietary FODMAPs (fermentable oligo-, di- and mono-saccharides and polyols) in healthy adults is poorly documented. This study compared specific effects of low and moderate FODMAP intake (relative to typical intake) on the faecal microbiome, participant-reported outcomes and gastrointestinal physiology. In a single-blind cross-over study, 25 healthy participants were randomised to one of two provided diets, ‘low’ (LFD) <4 g/d or ‘moderate’ (MFD) 14-18 g/d, for 3 weeks each, with ≥2-week washout between. Endpoints were assessed in the last week of each diet. The faecal bacterial/archaeal and fungal communities were characterised in 18 participants in whom high quality DNA was extracted by 16S rRNA and ITS2 profiling, and by metagenomic sequencing. There were no differences in gastrointestinal or behavioural symptoms (fatigue, depression, anxiety), or in faecal characteristics and biochemistry (including short-chain fatty acids). Mean colonic transit time (telemetry) was 23 (95% confidence interval: 15, 30) h with the MFD compared with 34 (24, 44) h with LFD (n=12; p=0.009). Fungal diversity (richness) increased in response to MFD, but bacterial richness was reduced, coincident with expansion of the relative abundances of Bifidobacterium, Anaerostipes, and Eubacterium. Metagenomic analysis showed expansion of polyol-utilising Bifidobacteria, and Anaerostipes with MFD. In conclusion, short-term alterations of FODMAP intake are not associated with symptomatic, stool or behavioural manifestations in healthy adults, but remarkable shifts within the bacterial and mycobiome populations were observed. These findings emphasise the need to quantitatively assess all microbial Domains and their interrelationships to improve understanding of consequences of diet on gut function.

Geoarchaeological research as part of the AHRC funded Living with Monuments (LwM) project investigated the upper Kennet river system across the Avebury World Heritage landscape. The results demonstrate that in the early–mid-Holocene (c. 9500–1000 bc) there was very low erosion of disturbed soils into the floodplain, with floodplain deposits confined to a naturally forming bedload fluvial deposit aggrading in a shallow channel of inter-linked deeper pools. At the time of the Neolithic monument building in the 4th–early 3rd millennium bc, the river was wide and shallow with areas of presumed braid plain. Between c. 4000 and 1000 bc, a human induced signature of soil erosion became a minor component of fluvial sedimentation in the Kennet palaeo-channel but it was small scale and localised. This strongly suggests that there is little evidence of widespread woodland removal associated with Neolithic farming and monument building, despite the evidently large timber requirements for Neolithic sites like the West Kennet palisade enclosures. Consequently, there was relatively light human disturbance of the hinterland and valley slopes over the longue durée until the later Bronze Age/Early Iron Age, with a predominance of pasture over arable land. Rather than large Neolithic monument complexes being constructed within woodland clearings, representing ancestral and sacred spaces, the substantially much more open landscape provided a suitable landscape with areas of sarsen spreads potentially easily visible. During the period c. 3000–1000 bc, the sediment load within the channel slowly increased with alluvial deposition of increasingly humic silty clays across the valley floor. However, this only represents small-scale landscape disturbance. It is from the Late Bronze Age–Early Iron Age when the anthropogenic signal of human driven alluviation becomes dominant and overtakes the bedload fluvial signal across the floodplain, with localised colluvial deposits on the floodplain margins. Subsequently, the alluvial archive describes more extensive human impact across this landscape, including the disturbance of loessic-rich soils in the catchment. The deposition of floodplain wide alluvium continues throughout the Roman, medieval, and post-medieval periods, correlating with the development of a low-flow, single channel, with alluvial sediments describing a decreasing energy in the depositional environment.

Approximately 80 million people live with chronic hepatitis B virus (HBV) infection in the WHO Africa Region. The natural history of HBV infection in this population is poorly characterised, and may differ from patterns observed elsewhere due to differences in prevailing genotypes, environmental exposures, co-infections, and host genetics. Existing research is largely drawn from small, single-centre cohorts, with limited follow-up time. The Hepatitis B in Africa Collaborative Network (HEPSANET) was established in 2022 to harmonise the process of ongoing data collection, analysis, and dissemination from 13 collaborating HBV cohorts in eight African countries. Research priorities for the next 5 years were agreed upon through a modified Delphi survey prior to baseline data analysis being conducted. Baseline data on 4,173 participants with chronic HBV mono-infection were collected, of whom 38.3% were women and the median age was 34 years (interquartile range 28–42). In total, 81.3% of cases were identified through testing of asymptomatic individuals. HBeAg-positivity was seen in 9.6% of participants. Follow-up of HEPSANET participants will generate evidence to improve the diagnosis and management of HBV in this region.

This paper focuses upon alterity and how we can more fully embrace intimations of otherness in our dealings with prehistoric monuments. Taking as its inspiration recent attempts to explain such structures, and the landscapes of which they were part, it makes two arguments. First, that while ethnographic analogies offer a vital point of departure for thinking through the possibilities raised by alterity and otherness, we may well have been overlooking a rich set of data—derived from careful excavation and painstaking metrical analyses—that has been sitting in front of us for a very long time. Second, despite over a decade of sustained critical debate, we seem remarkably timid when it comes to seeing where these data might take us. Through the lens of two Late Neolithic stone circles from southern Britain (one big, one small), research into measurement units and alignments is allied with recent excavation and survey data in order to explore ideas of hybridity, nomad-geometry and the arresting/manipulation of time and motion. Placing these glimpses of alterity front and centre, they are then used to establish new starting-points for the interpretation of these structures.

The Avebury henge is one of the famous megalithic monuments of the European Neolithic, yet much remains unknown about the detail and chronology of its construction. Here, the results of a new geophysical survey and re-examination of earlier excavation records illuminate the earliest beginnings of the monument. The authors suggest that Avebury's Southern Inner Circle was constructed to memorialise and monumentalise the site of a much earlier ‘foundational’ house. The significance here resides in the way that traces of habitation may take on special social and historical value, leading to their marking and commemoration through major acts of monument building.

Important Bird and Biodiversity Areas (IBAs) are sites identified as being globally important for the conservation of bird populations on the basis of an internationally agreed set of criteria. We present the first review of the development and spread of the IBA concept since it was launched by BirdLife International (then ICBP) in 1979 and examine some of the characteristics of the resulting inventory. Over 13,000 global and regional IBAs have so far been identified and documented in terrestrial, freshwater and marine ecosystems in almost all of the world’s countries and territories, making this the largest global network of sites of significance for biodiversity. IBAs have been identified using standardised, data-driven criteria that have been developed and applied at global and regional levels. These criteria capture multiple dimensions of a site’s significance for avian biodiversity and relate to populations of globally threatened species (68.6% of the 10,746 IBAs that meet global criteria), restricted-range species (25.4%), biome-restricted species (27.5%) and congregatory species (50.3%); many global IBAs (52.7%) trigger two or more of these criteria. IBAs range in size from < 1 km2 to over 300,000 km2 and have an approximately log-normal size distribution (median = 125.0 km2, mean = 1,202.6 km2). They cover approximately 6.7% of the terrestrial, 1.6% of the marine and 3.1% of the total surface area of the Earth. The launch in 2016 of the KBA Global Standard, which aims to identify, document and conserve sites that contribute to the global persistence of wider biodiversity, and whose criteria for site identification build on those developed for IBAs, is a logical evolution of the IBA concept. The role of IBAs in conservation planning, policy and practice is reviewed elsewhere. Future technical priorities for the IBA initiative include completion of the global inventory, particularly in the marine environment, keeping the dataset up to date, and improving the systematic monitoring of these sites.

The following paper aims to take a critical look at the role that can be played within the broad context of landscape based archaeological research by Geographical Information Systems (GIS). It will be argued that the rapid acceptance of GIS by archaeologists has not been without its problems, with a number of archaeologists wondering whether, despite the hype, any new approaches have been introduced at all. This, it will be argued, is a direct result of GIS-based applications tending to work within a largely inherited theoretical framework and, more importantly, lacking at present a critical theory of practice.

The aim of the paper is move beyond critique to suggest how GIS can provide not only an efficient means of generating simple distribution maps, but a flexible environment within which to bridge developments in theory and practice. Using an on-going case-study centred upon flood events in the palaeo-flood plain of the river Tisza, the implications of using GIS to welcome uncertainty into the analytical environment are explored and a number of approaches advocated. The significance these developments have in expanding our interpretive frameworks is explored through the fore-grounding and challenging of a number of dualistic modes of thought in that area actively encouraged and reinforced by the use of traditional GIS.

Although crop diversity has been identified as essential to enhance global food security and adapt to climate change, high loss of genetic resources is occurring due to agricultural industrialization and market requirements. Value chain development is an emerging market strategy that seeks to simultaneously achieve agrobiodiversity conservation and economic goals, though little empirical evidence exists regarding the extent to which value chains encourage biodiversity maintenance. This study considers the conservation of native potatoes among households in the highlands of Peru where value chain development is being pursued to create market niches for certain native potato varieties. Utilizing a mixed-methods case study approach, the findings of this study indicate that the conservers of native varieties are the households with more endowed resource bases as well as those that sell native varieties in value chains. However, the findings suggest that value chains themselves likely have only a marginal effect on conservation. Native potato conservation and potato production for value chains exist as two separate livelihood activities, and households with more resources are best positioned to engage in both. While value chains allow households to capitalize on the economic value of certain native varieties, the production of other native varieties allows households to fulfill cultural values. Based on these findings, this study concludes that value chain opportunities for native varieties should continue to be identified but they alone are not an adequate strategy to conserve agrobiodiversity. Therefore, in addition to value chain development, a full suite of conservation schemes should be implemented simultaneously.

This paper focuses upon the web of practices and transformations bound up in the extraction and movement of megaliths during the Neolithic of southern Britain. The focus is on the Avebury landscape of Wiltshire, where over 700 individual megaliths were employed in the construction of ceremonial and funerary monuments. Locally sourced, little consideration has been given to the process of acquisition and movement of sarsen stones that make up key monuments such as the Avebury henge and its avenues, attention instead focusing on the middle-distance transportation of sarsen out of this region to Stonehenge. Though stone movements were local, we argue they were far from lacking in significance, as indicated by the subsequent monumentalization of at least two locations from which they were likely acquired. We argue that since such stones embodied place(s), their removal, movement and resetting represented a remarkably dynamic and potentially disruptive reconfiguration of the world as it was known. Megaliths were never inert or stable matter, and we need to embrace this in our interpretative accounts if we are to understand the very different types of monument that emerged in prehistory as a result.

As a result of the exclusive use of extremely small megaliths (miniliths), the prehistoric stone settings of Exmoor, south-west England, challenge current approaches to the interpretation of monumental stone architecture during the later Neolithic and Early Bronze Age. Whilst the broader context of the practice of erecting tiny upright stones (a seemingly diverse and widespread phenomenon) and the reasons why this diminutive architecture has tended to escape sustained critical comment have been explored (smaller stone elements being relegated to a generalised background or subsidiary role such as ‘packing’), attempts to explain the settings have been remarkably few. Drawing upon the results of ten years of piecemeal fieldwork on the moor the present paper seeks to rectify this, arguing that far from being generalised ritual structures or metaphorical expressions of hunting groups, the tiny stones were, instead, an integral part of a dynamic human–animal landscape of movement and pause.

Depressed subjects have deficits in facialemotion recognition that resemble the deficits found in persons with focal right hemisphere brain damage. To locate the brain regions responsible for this problem, the authors imaged regional cerebral blood flow (rCBF) with H2O15 positron emission tomography in 10 mood-disordered patients, as well as in 10 age- and sex-matched healthy comparison subjects, while the subjects matched photographs for facial emotion or, as a control, facial identity. While matching faces for emotion, mood-disordered subjects had decreased rCBF activation bilaterally in their temporal lobes, as well as in the right insula, compared with healthy comparison subjects. Abnormal function of limbic and paraiimbic regions may partially explain the facial emotion-recognition deficits previously noted in depressed subjects.