The Pertosa Caves, today also known as the Pertosa-Auletta Caves, constitute an important karst system in the Campania region (southern Italy). Crossed by the waters of a river that re-emerges on the surface, they have an overall development of about 3 km. Thanks to the width of the entrance, the excellent location along a natural communication route through the mountains and the natural availability of water directly on the site, the initial part of the cavity was frequented by humans, without interruption, from prehistoric times to the Middle Ages. During the protohistory, in particular, the moment of most marked human presence is recorded in the cave. In this phase an extensive pile dwelling system was built on the waters of the underground river. The system was probably created to make the location, subject to frequent flooding, suitable for human settlement. This structure today constitutes an archaeological unicum not only in Italy but throughout Europe. We briefly analyze its general characteristics providing the results of a radiocarbon dating campaign which allowed to assess the occupation phases of the different contexts and the life span of the wooden artifacts, which came to us in a very good state of preservation. Radiocarbon data allowed to assess the chronological range of the human frequentation of the caves and to date the exceptionally well preserved underground pile dwelling system.

The Committee on Missing Persons in Cyprus (CMP) is a bicommunal committee with the mandate to locate and identify the skeletal remains of 2002 persons who were reported missing during the inter-communal fighting of 1963–64, as well as the events of July and August 1974. During the periods of conflict, several archaeological sites and old cemeteries were used as primary burial sites, among several other types of burials, as they were easily accessed by the persons involved in the interment and little effort was needed to conceal the bodies. The relatively large post-mortem interval and the generally poor post-mortem preservation of the skeletal remains poses an additional challenge in the forensic examination process and the identification of the remains, particularly in the absence of a context or other associated artifacts/evidence. Between 2016 and 2020, the CMP has been collaborating with CEDAD to clarify the relevancy of several cases by using radiocarbon dating. The CMP submitted 139 cases to CEDAD out of which 112 were determined as not linked to the 1963–64 and 1974 events and then not relevant for the CMP project. For the remaining samples radiocarbon dating was used to determine death age.

The stratigraphic succession of the Mar Piccolo basin (Gulf of Taranto, Southern Italy) is well known in the scientific literature dealing with the last interglacial since its morphological evolution is influenced by sea level changes during Late Pleistocene-Holocene. The local Holocene sea level history is well known thanks to data deriving from peat and ash layers identified in different sediment cores obtained underwater and in coastal areas. Peat sediments are frequently interlayed with muddy-sand beds rich in Cerastoderma glaucum (Poiret, 1789). In the literature of the Mediterranean basin, AMS 14C dating on C. glaucum is widely used also in paleo-environmental reconstruction because this bivalve is considered an useful marker of sea level, though in lagoonal systems, large age offsets have been reported in different areas. Due to the availability of precise chronological and geochronological markers, in order to validate the use of C. glaucum in paleo sea level reconstruction, AMS 14C dating campaign was carried out on this bivalve deriving from several cores drilled in the Mar Piccolo basin and its nearby areas. Nineteen AMS 14C dating analyses carried out on C. glaucum sampled from different sediment cores up to a maximum of 30 m from the seafloor are presented. These results show an inconsistency of the ages in relation to a sea-level rise reconstruction model. The interpretation of the data was performed after the estimation of the local age offset calculated by analyzing six live samples, collected in 2017 in Mar Piccolo and in Croatia, and two samples dated to 1968–1969. The results show that for both the classes of samples (2017 and 1960s) an age offset ranging from 600 to 800 yr can be estimated.

Combined dendrochronology and accelerator mass spectrometry radiocarbon (AMS 14C) dating analyses were used in order to date an old living tree named Italus, growing in the Pollino massif in Southern Italy. Wiggle match AMS 14C dating analysis was performed on a 320-yr-long floating chronology obtained by cross-dating four wood cores extracted from the exposed roots of the tree. Following this approach, an age for the tree of ≈1230 yr was estimated. This age makes Italus the oldest living tree in Europe. High-resolution 14C dating analyses performed on single rings extracted from the tree stem allowed us to identify the 993–994 CE large excursion in atmospheric 14C concentration (Miyake event) revealing for the first time its presence in the Mediterranean basin.

In this paper, we present the results of the accelerator mass spectrometry radiocarbon (AMS14C) dating campaign performed on samples selected from different levels in Grotta Romanelli (Castro, Italy). Grotta Romanelli is one of the key sites for the chronology of Middle Pleistocene–Holocene in Mediterranean region. After the first excavation campaigns carried out in the first decades of the 1900s, the cave has been systematically re-excavated only since 2015. During the last excavation campaigns different faunal remains were selected and submitted for 14C dating in order to confirm the chronology of the cave with a higher resolution. Isotopic ratio mass spectrometry (IRMS) measurements were also carried out on faunal remains.

The determination of the proportion between the biogenic and the fossil-derived fraction in carbon dioxide emissions from industrial stationary sources is a relevant aspect in the frame of the worldwide efforts to reduce greenhouse gas emissions into the atmosphere. In this field the 14C-based method has gained importance over the years and has been included in standard protocols accepted at both the national and international levels. The advantages of the method, based on the large difference in terms of 14C signature between fossil and biogenic carbon, are reviewed as well as some critical aspects related to its application. In particular the results of a study aimed at validating the method by analyzing synthetic gas mixtures produced in a dedicated plant and with a tuneable proportion between fossil and biogenic carbon dioxide are presented.

Archaeological surface surveys carried out near Salve in southern Italy led to the identification of about 90 stone mounds spread over an area of about 100 ha. Systematic archaeological investigations allowed to identify the mounds as funeral structures with some having megalithic features. In the necropolis, both the inhumation and cremation rituals are evident, in some cases within the same mound. This article presents the results of an accelerator mass spectrometry (AMS) radiocarbon dating campaign carried out on different kinds of samples recovered from several structures: unburned and cremated bones, charcoals, and organic residues extracted from pottery sherds. The results allowed to assess the chronology of the site and to shed new light on the different funeral practices in Copper Age southern Italy.

Grotta della Monaca is a karstic cave in Calabria (southern Italy) that plays an important role in reconstructing the oldest strategies for the acquisition of mineral resources in the Mediterranean. In fact, systematic archaeological excavations carried out by the University of Bari allowed the identification of intense prehistoric mining activities aimed at the exploitation of iron and copper ores. Archaeological evidence suggests different phases of frequentation of the cave spanning from the Upper Paleolithic, Neolithic, Copper and Bronze ages up to the Middle Ages. In order to establish an absolute timeframe for the different phases, a radiocarbon dating campaign was carried out and the results presented in this paper.

Accelerator mass spectrometry (AMS) radiocarbon analyses were performed on biobased unsaturated polyester resins in order to assess the potential of the method for the determination of the bio-fraction. Different resins were synthesized in the laboratory with different proportions of raw materials of biogenic origin, and analyzed both in the liquid and in the solid form. As a preliminary step of the study, both the biobased and the fossil-derived raw materials were analyzed in order to determine their 14C content. A comparison of the obtained results with the expected ones allowed us to investigate the potential of the 14C method in this field as well as to address some still open issues such as the levels of uncertainty and accuracy as related to the calculation procedures, the correction for isotopic fractionation, and the effect of the presence of volatile components in the analyzed materials.

Various samples extracted from human tissues (with different radiocarbon turnover rates) of a post-bomb human body were submitted to accelerator mass spectrometry (AMS) 14C dating: hair; a cortical fraction of a skull bone; a trabecular fraction of a pubic symphysis; and enamel extracted from permanent teeth with different dates of formation were analyzed. The analyzed samples showed varying 14C concentrations corresponding to different times of formation or different turnover rates. The implications of the results in forensics studies are discussed.

In the sample preparation laboratory of CEDAD (CEnter for DAting and Diagnostics) of the University of Lecce, a protocol for the quality control of bone samples based on infrared spectroscopy has been set up. The protocol has been recently developed as a check-in test with the aim to identify the presence of collagen in the samples, assess its preservation status, and determine whether the submitted bone samples are suitable for accelerator mass spectrometry (AMS) radiocarbon measurements or not. We discuss in this paper the use of infrared-based techniques to identify the presence of “contaminants” such as restoration and consolidation materials, humic acids, and soil carbonates, which, if not removed by the sample processing chemistry, can be sources of exogenous carbon and can thus influence the accuracy of the 14C determinations.

Bone samples recovered in well-defined and previously dated archaeological contexts were intentionally contaminated, submitted to the standard method for collagen extraction and purification, and then characterized by means of Fourier transform infrared (FTIR) spectroscopy analyses performed in attenuated total reflection (ATR) mode before being combusted, converted to graphite, and measured by AMS. The study shows that the ATR-FTIR technique is an extremely powerful method for the identification of both the collagen and its contaminants and can supply important information during the selection and processing of samples to be submitted for 14C dating.