Objectives/Goals: Hospital mergers have proliferated in the USA, yet a comprehensive understanding of how mergers affect nurses’ well-being and their ability to provide safe, high-quality care is lacking. We examined the associations among hospital mergers, nursing, nurse well-being, and patient safety and quality of care to guide policy or hospital interventions. Methods/Study Population: Following the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for scoping reviews reporting guidelines, we conducted a scoping review by systematically searching 10 databases comprised of both business and healthcare literature up to January 2025. Eligible studies met the following inclusion criteria: (1) US-based sample, (2) specify a hospital merger and one nursing-related criteria, (3) use empirical data, (4) peer-reviewed, and (5) in English. Conference abstracts, protocols, interventions, non-empirical studies, theses or dissertations, quality improvement, and systematic review articles were excluded. Two reviewers independently screened articles and extracted data to focus on empirical research that examined hospital mergers and nursing. Results/Anticipated Results: Our search yielded 1,772 articles; 10 met our inclusion criteria and were included in the analysis: 5 quantitative, 3 qualitative, and 2 multi-methods. Only three studies analyzed data from 2003 or later. Although the data were sparse and older, we found that hospital mergers introduced organizational changes among the nursing workforce – nurse staffing, leadership, and operations. Nurse-sensitive patient safety and quality of care outcomes may not improve post-merger. Qualitatively, nurses described poor communication, a perceived lack of trust in leadership, a loss of identity and unity, decreased morale, increased fear or uncertainty, and issues with equal and/or respectful treatment. The identified study methods were unable to draw strong causal and/or detailed conclusions, and few variables were examined. Discussion/Significance of Impact: This review highlights potential links between US hospital mergers and nursing, nurse well-being, and patient safety and quality of care and emphasizes the lack of robust, up-to-date studies to guide policymakers and hospital administrators in making informed decisions – potentially leading to negative consequences.