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The purpose of surgical hand antisepsis before surgical or invasive procedures is to suppress microbial flora on the hands. This study aimed to evaluate the effect of a modified surgical hand antisepsis technique on recolonization of microbial flora on surgeons’ hands over time.
Methods:
Participating surgeons were randomly assigned to one of two study groups. In both groups, surgical hand antisepsis was performed using 10% povidone-iodine followed by rinsing of the hands and forearms. In Group 1, surgeons dried their hands after rinsing according to the conventional method. In Group 2, surgeons applied an additional layer of 10% povidone-iodine to the still-wet hands and forearms after rinsing and then allowed the solution to dry without further rinsing. Hand cultures were obtained immediately after antisepsis (0 hour) and at 1 and 2 hours thereafter. Colony-forming units (CFUs) were counted and compared between groups and sampling periods.
Results:
Fourteen surgeons participated in the study, with seven surgeons allocated to each group. No significant difference in bacterial growth was observed between the groups at 0 hour (Group 1: mean CFU 41.59 ± 72.53 vs Group 2: 18.63 ± 24.27, P = .27). At 1 hour, bacterial growth was significantly lower in Group 2 compared with Group 1 (8.43 ± 20.65 vs 25.44 ± 53.62 CFU, P = .001). Similarly, at 2 hours, Group 2 demonstrated significantly lower bacterial growth than Group 1 (16.37 ± 34.25 vs 32.88 ± 52.55 CFU, P = .022).
Conclusion:
Compared with the conventional surgical handwashing technique, reapplication of povidone-iodine without a final rinse resulted in significantly reduced bacterial recolonization on surgeons’ hands at 1 and 2 hours after hand antisepsis. These findings suggest that this modified technique may provide prolonged antimicrobial activity during surgical procedures. Further large-scale studies are warranted to confirm its clinical utility.
This study presents a very cost effective template-based electrochemical technique to synthesize the nano heterojunctions of cadmium sulfide (CdS) and cadmium telluride (CdTe). SEM analysis revealed that the average length of CdS nanowires varied from 500 nm to 4 μm depending on the deposition time and voltage. Also, average diameter of the CdS nanowires ranged between 100 and 200 nm. The structures of CdS nanowires have been confirmed by XRD and EDX analysis. Photoelectrochemical performances of CdS nanowires revealed that there is a dramatic change in the photoelectrochemical performances with the change in deposition time and voltage. The maximum fill factor (FF) and power efficiency (η) of the CdS nanowires has been calculated as 45 % and 1.36 %, respectively. After the optimization of the CdS nanowire deposition conditions based on the PEC performance, CdTe nanostructures have been deposited on CdS nanowires at various deposition time. SEM analysis showed that CdTe nanostructures have a tendency to grow as nanoclusters. It was observed that the density and the average diameter of the clusters was a strong function of the deposition time. The average diameter of the CdTe nanoclusters after 9-hour deposition reached about 260 nm. The successful heterojunction of CdS nanowires with CdTe nanoclusters have been observed in the SEM analysis. It has been concluded that the PEC performances of the CdS nanowires improved significantly after CdTe deposition. The maximum η, obtained in this study is about 8.04 %. This is one of the highest efficiencies reported in the literature for the nanowire array photoelectrochemical cells.
A 67-year-old man with an enlarged left tonsil underwent a tonsillectomy for the suspicion of malignancy. He had had a dental extraction six months earlier. Clinical and histopathological investigations established the diagnosis of primary tonsillar tuberculosis.
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