For digital light microscopes, which have image sensors but no eyepieces, the image is displayed directly on an electronic monitor. This development brings a significant change to the usual way magnification of an object viewed via a microscope is determined. Over the years, there have been various standards that define magnification when viewing an image through a microscope’s eyepieces. Only recently have magnification standards been developed that apply to a digital microscope. This article offers guidelines for determining the range of useful magnification values for digital microscopy, particularly when monitors of various sizes are employed.