Atomic Force Microscopy (AFM) has developed into a very powerful tool for characterization of surfaces and nanoscale objects. Many physical properties of an object can be studied by AFM with nanometer-scale resolution. Local stiffness, elasticity, conductivity, capacitance, magnetization, surface potential and work function, friction, piezo response—these and many other physical properties can be studied with over 30 AFM modes. What is typically lacking in information provided by AFM studies is the chemical composition of the sample and information about its crystal structure. To obtain this information other characterization techniques are required, such as Raman and fluorescence microscopy. The Raman effect (inelastic light scattering) provides extensive information about sample chemical composition, quality of crystal structure, crystal orientation, presence of impurities and defects, and so on. Information provided by Raman and fluorescence spectroscopy is complementary to the information obtained by AFM. So it is a natural requirement in many research fields to integrate these techniques in one piece of equipment—to provide comprehensive physical, chemical, and structural characterization of the same object. Of course, for routine studies of various samples, it is important to be able to obtain AFM and Raman/fluorescence images of exactly the same sample area, preferably with the same sample scan.