Skip to main content Accessibility help
×

Temporary Disruption

We have implemented additional assurance measures to protect our systems.

As a result, we have suspended some of our systems and services. We apologise for any temporary disruption some customers may experience.

  1. Home
  2. Search

Refine search

Refine search

Access:
Content type:
Publication date:
Apply   From and To filters
Subject: Show more
Journals Show more
Publishers: Show more
Societies Show more
Series: Show more
Collections: Show more

Actions for selected content:

Select all | Deselect all
  • View selected items
  • Export citations
  • Download PDF (zip)
  • Save to Kindle
  • Save to Dropbox
  • Save to Google Drive

Save Search

You can save your searches here and later view and run them again in "My saved searches".

Please provide a title, maximum of 40 characters.
Cancel
×

115 results

Page 1 of 6

  • Chapter 25 - Sperm Selection for ICSI by Morphology

  • from Section 4 - Insemination/ICSI
  • Edited by Markus H. M. Montag, ilabcomm GmbH, St Augustin, Germany, Dean E. Morbeck, Kindbody Inc, New York City
  • Book:
    Principles of IVF Laboratory Practice
    Published online:
    07 August 2023
    Print publication:
    24 August 2023, pp 186-195

  • Summary

    Spectacular progress in the therapeutic management of patients with severe male infertility was obtained with the implementation of intracytoplasmic sperm injection (ICSI) and has opened avenues to treating previously untreatable cases of infertility. However, it must be recognized that in the vast majority of ICSI cycles, the selection of the spermatozoon followed by the intracytoplasmic injection is done very quickly at low magnification and without detection possibilities for potential pathological morphological anomalies of spermatozoa. Strong correlations between sperm morphological characteristics and male fertility have been demonstrated. Thus, it seems reasonable to implement optimized non-invasive sperm selection techniques in ART based on morphological ’normalcy’ of spermatozoa. Almost 20 years ago, ’motile-sperm organelle morphology examination’ (MSOME) was introduced by Bartoov et al. Using Nomarski differential interference contrast optics (DIC) an improved three-dimensional view of the head and midpiece became available. This chapter will describe different aspects of sperm selection by morphology.

  • Chapter 5 - Morphological Sperm Selection Before ICSI

  • Edited by Gianpiero D. Palermo, Zsolt Peter Nagy
  • Book:
    Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction
    Published online:
    02 December 2021
    Print publication:
    25 November 2021, pp 49-58

  • Summary

    Application of intracytoplasmic morphologically selected sperm injection (IMSI) is a matter of debate; however, it is evident that deselection of spermatozoa presenting respectively spermatozoa with vacuole-like structures and asymmetrical insertion of the neck has multiple benefits. Defective sperm function and molecular defects contribute to early embryonic development disturbance, failure of blastocyst formation, miscarriages, and congenital birth defects. Introduction of IMSI has made embryologists aware that for ICSI the selection of sperm should be given proper attention. We have to consider IMSI has improved the ICSI technique. Compared to “blind” spermatozoa aspiration at low magnification and/or reduced optics contrast, observation under optimal contrast optics increases the probability of selecting a normal spermatozoa. This is particularly evident in cases of severe teratozoospermia, with low responder and advanced age women. Practicable and technical aspects of morphologically normal sperm selection must be considered to facilitate the workflow while performing IMSI and avoiding damage to the oocytes. A proper set up of the dish for selecting and capturing sperm facilitates routine application of IMSI.

  • Spindle and chromosome configuration analysis of human biopsied versus non-biopsied embryos by confocal laser scanning microscopy following vitrification

  • Katerina Chatzimeletiou, Pierre Vanderzwalmen, Yannis Panagiotidis, Achilleas Papatheodorou, Alexandros Karagiannidis, Efstratios Kolibianakis, Grigoris Grimbizis, Nikos Prapas, Yannis Prapas, Basil C. Tarlatzis
  • Journal:
    Zygote / Volume 27 / Issue 3 / June 2019
    Published online by Cambridge University Press:
    07 May 2019, pp. 153-159

  • The aim of this study was to investigate the effects of zona drilling and biopsy on day 3 followed by vitrification on day 5 on the cytoskeleton and development of human embryos, by analysing survival rates and spindle and chromosome configurations by fluorescence and confocal laser scanning microscopy in human biopsied and non-biopsied embryos. In total, 98 human blastocysts (50 non-biopsied and 48 following biopsy on day 3) were vitrified on day 5 using either a commercial dimethyl sulphoxide (DMSO)-free vitrification kit or increasing concentrations of DMSO/EG (5%/5–10%/10–20%/20%). Following warming, the blastocysts were allowed to recover in culture for 24 h and were immunostained with α-tubulin, acetylated tubulin, and/or γ-tubulin antibodies in combination with 4′,6-diamidino-2-phenylindole (DAPI). Labelled embryos were examined by both fluorescence and confocal laser scanning microscopy. The survival rates following warming (92% non-biopsied vs 83.3% biopsied) and the incidence of normal spindle chromosome configurations was not statistically different between the two groups (65.2% non-biopsied vs 59.2% biopsied, P>0.05). The incidence of spindle abnormalities including multipolarity, chromosome lagging, congression failure and chromosome bridging were also similar between the two groups (P>0.05). This study is the first to compare the incidence of cytoskeletal abnormalities in biopsied and non-biopsied human embryos following vitrification. We conclude that there was no significant difference in the survival rates and the incidence of spindle abnormalities between the two groups.

Atlas of Vitrified Blastocysts in Human Assisted Reproduction
  • Thomas Ebner, Pierre Vanderzwalmen, Barbara Wirleitner
  • Published online:
    05 May 2015
    Print publication:
    16 April 2015
  • The method of vitrification of oocytes and embryos is fundamental for the outcome of IVF. This atlas presents data on both closed system and open vitrification techniques, and the consequences of each method for survival rates, aiding the comparison of vitrification methods. Structured on a patient-by-patient basis, the atlas describes 100 clinically documented case studies that follow the evolution of cryopreserved blastocysts between warming and blastocyst transfer. It relates fresh to post-warming blastocyst morphology and to response to controlled ovarian hyperstimulation. For each case, pronuclear morphology and synchrony, as well as embryo morphology, are reported and described. Data on indications for treatment, stimulation type and duration, are accompanied by over 400 high-quality images of vitrified blastocysts. Covering the state-of-the-art techniques, this atlas is an essential aid in selecting the vitrification method for clinical embryologists and physicians in reproductive medicine.

Page 1 of 6