Uptake of cage-free egg production in China remains limited, and many food companies have yet to meet their 2025 cage-free commitments. As the fulfilment of cage-free commitments potentially stimulates the growth of cage-free production, it is important to understand the factors influencing corporates’ implementation. Drawing on seven semi-structured interviews with representatives from international food businesses and certification bodies operating in China, this study explores the enablers and challenges to implementing 2025 cage-free egg commitments and the role of certification schemes in supporting supply chain transitions. The findings show that access to cage-free certification schemes has supported the development of cage-free egg production and procurement. However, limited traceability and the absence of government regulation on animal welfare and product labelling remain challenges. Key enablers identified include the establishment of cage-free commitments, internal alignment and organisational buy-in, and phased implementation strategies. Despite this, major constraints persist, including high procurement costs, the complexity of transitioning multi-tier supply chains, and concerns about supply stability and continuity. While long-term interventions, such as policy development, producer capacity building, and consumer education, are essential, short-term market-based mechanisms, particularly the cage-free egg credits scheme, may offer a practical interim solution. These schemes can help companies fulfil their commitments and provide financial incentives for producers, thereby accelerating the transition to cage-free production and advancing animal welfare in China.

In this study, the Mach reflection of a detonation wave in 2H$_2$ + O$_2$ + 2Ar over a concave double wedge was experimentally investigated. Three Mach reflection configurations could be observed successively, namely, Mach reflection of a Chapman–Jouguet detonation over the first surface, Mach reflection of an overdriven detonation wave over the second surface, and Mach reflection after the interaction of two triple points. The experimental results indicated that the classical reactive theories based on the straight Mach stem assumption are unable to accurately predict the behaviours of the latter two Mach reflections. The asymptotic triple-point trajectory angle of the secondary Mach reflection, $\chi _2$, is significantly smaller than the theoretical prediction. A curved model for the Mach stem $m_1$ is constructed, introducing a curved Mach stem near the triple point. The Mach stem has a concave curvature, implying a lower overdrive degree and a smaller incident angle, which consequently leads to a reduction in $\chi _2$. Based on the curved Mach stem model, an equivalent wedge angle $\varPhi$ and an equivalent overdrive degree $\overline {\alpha }$ are introduced to predict the value of $\chi _2$. An analytical model for solving the interaction of two triple points was developed. Two different wave structures after the interaction – namely, downward-travelling shock–shock and downward-travelling centred shock–expansion – were determined using gas dynamic and shock dynamic methods. The curved Mach stem $m_1$ can result in the variation of the wave structure.

Ultrafast vortex beams are essential in many scientific and industrial fields owing to their unique spatial and temporal characteristics. We demonstrate a passively mode-locked ytterbium-doped yttrium aluminum garnet (Yb:YAG) thin-disk oscillator that directly generates high-power femtosecond vortex beams with excellent beam quality. Using Kerr-lens mode-locking assisted by a semiconductor saturable absorber mirror, we achieve 594 fs Laguerre–Gaussian vortex pulses with 50 W average power at a 68.5 MHz repetition rate, representing the highest power reported for a femtosecond vortex oscillator. By replacing the planar output coupler with a coated axicon, we further realize stable mode-locking and efficient generation of first-order Bessel vortex beams. This compact and integrated design eliminates external mode converters, enabling robust generation of high-power ultrafast structured light. The demonstrated source provides a promising platform for precision material processing, strong-field vortex dynamics and quantum optical studies.

To determine values for the digestible indispensable amino acid score (DIAAS), it is recommended that ileal amino acid (AA) digestibility values obtained in growing pigs are used to characterise protein quality in different foods. Therefore, an experiment was conducted to determine the standardised ileal digestibility (SID) of AA in eight energy ingredients (barley, sorghum, wheat, brown rice, rice bran, wheat bran, cassava and paddy rice) fed to pigs, where SID values in pigs can be used to calculate approximate DIAAS values in humans. Among the data obtained for all energy ingredients, significant variations (P < 0·01) in CP and AA composition were observed. Rice bran and wheat bran had the highest CP (16·43 % and 18·16 %, respectively) and DIAAS scores of 81–88 for adult, qualifying as ‘good’ protein sources (> 75). Cassava, with the lowest CP (2·74 %), was limited by sulphur amino acid (54). Lysine (Lys) was the first-limiting AA in barley (74), sorghum (51) and wheat (49), with SID values lowest in wheat (71·04 %). Brown rice and paddy rice showed higher SIDLys (87·51 % and 78·13 %, respectively). These findings highlight the potential of bran-based ingredients and Lys fortification to improve protein quality in grain-dependent diets, providing the scientific basis to combat protein malnutrition.

Although deep reinforcement learning (DRL) techniques have been extensively studied in the field of robotic manipulators, there is limited research on directly mapping the output of policy functions to the joint space of manipulators. This paper proposes a motion planning scheme for redundant manipulators to avoid obstacles based on DRL, considering the actual shapes of obstacles in the environment. This scheme not only accomplishes the path planning task for the end-effector but also enables autonomous obstacle avoidance while obtaining the joint trajectories of the manipulator. First, a reinforcement learning framework based on the joint space is proposed. This framework uses the joint accelerations of the manipulator to calculate the Cartesian coordinates of the end-effector through forward kinematics, thereby performing end-to-end path planning for the end-effector. Second, the distance between all the linkages of the manipulator and irregular obstacles is calculated in real time based on the Gilbert–Johnson–Keerthi distance algorithm. The reward function containing joint acceleration is constructed with this distance to realize the obstacle avoidance task of the redundant manipulator. Finally, simulations and physical experiments were conducted on a 7-degree-of-freedom manipulator, demonstrating that the proposed scheme can generate efficient and collision-free trajectories in environments with irregular obstacles, effectively avoiding collisions.

With extraordinary preservation, the bivalved Cassicaris clarksoni gen. et sp. nov. from the early Cambrian (Stage 3) Xiaoshiba Lagerstätte in Kunming, China, is characterised by having an anterior cardinal spine and a heavily segmented body, including an abdomen with a heavily sclerotised shell. Anatomically, the antennulae are small and the antennae are robust with seta-bearing podomeres, possibly of predatory function, followed by the other five pairs of biramous cephalic limbs. There are about 11 pairs of thorax segments, each corresponding to a pair of biramous limbs, including a multi-segmented endopod with feather-like podomeres and terminal spines, and a small paddle-shaped exopod fringed with setae. The bulbous, stalked eyes, which exhibit fineness of vision, infer adaptation to a vagile epibenthic lifestyle. Functionally, such assorted appendages may indicate an efficient suspension-feeding strategy for capturing tiny zooplankton. The median eye is presumably not a typical ocellar system but another compound eye, which may offer further insights into the evolution of compound eyes. Cladistic analysis implies that Cassicaris is a sister taxon to Pectocaris and Jugatacaris; these intriguing euarthropods are critical for discerning their body plan and living habits. Our findings offer fresh insights into the early evolution of Cambrian euarthropods, characterised by notable morphological disparity and ecological diversity. These fossils, including not only many intact individuals but also a few with well-preserved soft parts, form well-characterised groupings, making the broad pattern of Cambrian arthropod systematics increasingly consensual.

Visual exploration is a task in which a camera-equipped robot seeks to efficiently visit all navigable areas of an environment within the shortest possible time. Most existing visual exploration methods rely on a static camera fixed to the robot’s body to control its own movements. However, coupling the orientation of camera with robot’s body limits the extra degrees of freedom to obtain more visual information. In this work, we adjust the camera orientation during robot motion by using a novel camera view planning (CVP) policy to improve the exploration efficiency. Specifically, we reformulate the CVP problem as a reinforcement learning problem. However, two new challenges need to be addressed: 1) determining how to learn an effective CVP policy in complex indoor environments and 2) figuring out how to synchronize it with the robot motion. To solve the above issues, we create a reward function considering factors such as exploration area, observed semantic objects, and the motion conflicts between the camera and the robot’s body. Moreover, to better coordinate the policies of the camera and the robot’s body, the CVP policy takes the body actions and the egocentric 2D spatial maps with exploration, occupancy, and trajectory information into account to make motion decisions. Experimental results show that after using the proposed CVP policy, the exploration area is expanded by 21.72% and 25.6% on average in the small-scale indoor scene with few structured obstacles and large-scale indoor scene with cluttered obstacles, respectively.

The transition from conventional cage systems to cage-free egg production in China remains limited despite apparently increasing consumer demand for cage-free eggs. This study interviewed 15 large-scale Chinese egg producers using cages and/or cage-free systems (i.e. single-, multi-tier and free-range) to investigate the perceived challenges and opportunities during the transition. The cage farms’ scales range from 110,000 to 30 million, while the cage-free farms keep between 12,000 and 300,000 laying hens. Drawing upon the COM-B model of the Behaviour Change Wheel, this study explored how producers’ Capabilities, Opportunities, and Motivations impact decision-making processes. Key findings reveal that cage and cage-free producers considered consumer demand and profitability as primary drivers for adopting cage-free systems. While free-range producers were more confident in the market, barn system producers faced greater uncertainty due to limited engagement from corporate buyers. Moreover, these cage-free producers believed reliable certification and labelling schemes to be critical for building consumer trust and ensuring the success of cage-free operations. All the participants perceived access to sufficient land and financial resources to be essential for a successful transition. While most studies propose education as a long-term strategy to promote the growth of the cage-free egg sector, our findings are the first to highlight that engaging corporate buyers and establishing trustworthy certification schemes are the most crucial short-term interventions required to drive the development of large-scale cage-free farms and support sustained improvements in animal welfare in China.

Linear non-modal analyses are performed to study the mechanism of how deformable free surfaces influence very-large-scale motions (VLSMs) in turbulent open channel flows. The mean velocity and eddy viscosity profiles obtained from direct numerical simulations are used in the generalised Orr–Sommerfeld and Squire equations to represent background turbulence effects. Solutions of surface-wave eigenmodes and shear eigenmodes are obtained. The results indicate that at high Froude numbers, free surfaces enhance the maximum transient growth rate of VLSMs through surface-wave eigenmodes. We then analyse the energy budget equation to reveal the underlying mechanism. For streamwise-uniform motions, the energy growth rate is enhanced by an energy production term associated with the correlation between the streamwise velocity, which is generated by the lifting-up effect of streamwise vortices composed of shear eigenmodes, and the vertical velocity, which is induced by a spanwise standing wave composed of surface-wave eigenmodes. For streamwise-varying motions, the energy growth rate is enhanced by a standing wave moving with a pair of vortices that travel at a speed approximately equal to the projection of the mean surface velocity along the wavenumber vector direction. Finally, an analytical expression of the energy production term is derived to provide the initial conditions for the maximum transient growth and explain the weak free-surface effect observed at large spanwise wavenumbers and low Froude numbers. The results demonstrate a linear non-modal mechanism in interactions between free surfaces and VLSMs in open channel flows.

The potential threshold for dietary energy intake (DEI) that might prevent protein-energy wasting (PEW) in chronic kidney disease (CKD) is uncertain. The subjects were non-dialysis CKD patients aged ≥ 14 years who were hospitalised from September 2019 to July 2022. PEW was measured by subjective global assessment. DEI and dietary protein intake (DPI) were obtained by 3-d diet recalls. Patients were divided into adequate DEI group and inadequate DEI group according to DEI ≥ 30 or < 30 kcal/kg/d. Logistic regression analysis and restricted cubic spline were used in this study. We enrolled 409 patients, with 53·8 % had hypertension and 18·6 % had diabetes. The DEI and DPI were 27·63 (sd 5·79) kcal/kg/d and 1·00 (0·90, 1·20) g/kg/d, respectively. 69·2 % of participants are in the inadequate DEI group. Malnutrition occurred in 18·6 % of patients. Comparing with patients in the adequate DEI group, those in the inadequate DEI group had significantly lower total lymphocyte count, serum cholesterol and LDL-cholesterol and a higher prevalence of PEW. For every 1 kcal/kg/d increase in DEI, the incidence of PEW was reduced by 12·0 % (OR: 0·880, 95 % CI: 0·830, 0·933, P < 0·001). There was a nonlinear curve relationship between DEI and PEW (overall P < 0·001), and DEI ≥ 27·6 kcal/kg/d may have a preventive effect on PEW in CKD. Low DPI was also significantly associated with malnutrition, but not when DEI was adequate. Decreased energy intake may be a more important factor of PEW in CKD than protein intake.

This paper retrospectively analysed the prevalence of macrolide-resistant Mycoplasma pneumoniae (MRMP) in some parts of China. Between January 2013 and December 2019, we collected 4,145 respiratory samples, including pharyngeal swabs and alveolar lavage fluid. The highest PCR-positive rate of M. pneumoniae was 74.5% in Beijing, the highest resistance rate was 100% in Shanghai, and Gansu was the lowest with 20%. The highest PCR-positive rate of M. pneumoniae was 74.5% in 2013, and the highest MRMP was 97.4% in 2019; the PCR-positive rate of M. pneumoniae for adults in Beijing was 17.9% and the MRMP was 10.48%. Among the children diagnosed with community-acquired pneumonia (CAP), the PCR-positive and macrolide-resistant rates of M. pneumoniae were both higher in the severe ones. A2063G in domain V of 23S rRNA was the major macrolide-resistant mutation, accounting for more than 90%. The MIC values of all MRMP to erythromycin and azithromycin were ≥ 64 μg/ml, and the MICs of tetracycline and levofloxacin were ≤ 0.5 μg/ml and ≤ 1 μg/ml, respectively. The macrolide resistance varied in different regions and years. Among inpatients, the macrolide-resistant rate was higher in severe pneumonia. A2063G was the common mutation, and we found no resistance to tetracycline and levofloxacin.

Deubiquitinases are a group of proteins that identify and digest monoubiquitin chains or polyubiquitin chains attached to substrate proteins, preventing the substrate protein from being degraded by the ubiquitin-proteasome system. Deubiquitinases regulate cellular autophagy, metabolism and oxidative stress by acting on different substrate proteins. Recent studies have revealed that deubiquitinases act as a critical regulator in various cardiac diseases, and control the onset and progression of cardiac disease through a board range of mechanism. This review summarizes the function of different deubiquitinases in cardiac disease, including cardiac hypertrophy, myocardial infarction and diabetes mellitus-related cardiac disease. Besides, this review briefly recapitulates the role of deubiquitinases modulators in cardiac disease, providing the potential therapeutic targets in the future.

There is a trend towards the adoption of cage-free housing systems in the egg industry across Asia. While cage-free housing systems can hold significant animal welfare advantages over cages, there can also be challenges in managing these systems. This exploratory study aimed to investigate the perspectives of egg producers on the main challenges and proposed solutions associated with cage-free systems in China, Indonesia, Thailand, Japan, Malaysia, and the Philippines. Cage-free producers found disease prevention and maintaining a healthy profit margin more difficult than producers from cage farms, while it was less difficult to provide environmental enrichment in cage-free systems compared to cage farms. The top challenges for cage-free producers were the cost of production, system management, disease, sales, and egg production, and the top proposed solution was to improve on-farm practices and efficiencies. Eighty-one percent of egg producers believed that more support is needed to maintain their farms than is currently available, and support was most needed in helping to improve sales, improve farm operations, lower farm costs, and provide information for producers in the form of education and training. Most responses identified the government as the stakeholder that should offer support. These results may help direct further studies in this field as well as supplying information to develop relevant initiatives with an emphasis on education and training, thereby improving animal welfare on cage-free farms and increasing the uptake of high welfare cage-free farms across the region.

The oscillatory Kelvin–Helmholtz (K–H) instability of a planar liquid sheet was experimentally investigated in the presence of an axial oscillating gas flow. An experimental system was initiated to study the oscillatory K–H instability. The surface wave growth rates were measured and compared with theoretical results obtained using the authors’ early linear method. Furthermore, in a larger parameter range experimentally studied, it is interesting that there are four different unstable modes: first disordered mode (FDM), second disordered mode (SDM), K–H harmonic unstable mode (KHH) and K–H subharmonic unstable mode (KHS). These unstable modes are determined by the oscillating amplitude, oscillating frequency and liquid inertia force. The frequencies of KHH are equal to the oscillating frequency; the frequency of KHS equals half the oscillating frequency, while the frequencies of FDM and SDM are irregular. By considering the mechanism of instability, the instability regime maps on the relative Weber number versus liquid Weber number (Werel–Wel) and the Weber number ratio versus the oscillating frequency (Werel/Wel–$\varOmega$s2) were plotted. Among these four modes, KHS is the most unexpected: the frequency of this mode is not equal to the oscillating frequency, but the surface wave can also couple with the oscillating gas flow. Linear instability theory was applied to divide the parameter range between the different unstable modes. According to linear instability theory, K–H and parametric unstable regions both exist. However, note that all four modes (KHH, KHS, FDM and SDM) corresponded primarily to the K–H unstable region obtained from the theoretical analysis. Nevertheless, the parametric unstable mode was also observed when the oscillating frequency and amplitude were relatively low, and the liquid inertia force was relatively high. The surface wave amplitude was small but regular, and the evolution of this wave was similar to that of Faraday waves. The wave oscillating frequency was half that of the surface wave.

The low maturation rate of oocytes is an important reason for female infertility and failure of assisted pregnancy. The germinal vesicle breakdown (GVBD) is a landmark event of oocyte maturation. In our previous studies, we found that zona pellucida 3 (ZP3) was strongly concentrated in the nuclear region of germinal vesicle (GV) oocytes and interacted with aryl hydrocarbon receptor-interacting protein-like 1 (AIPL1) and lamin A to promote GVBD. In the current study, we found that lamin A is mainly concentrated in the nuclear membrane. When ZP3 is knocked down, lamin A will be partially transferred to the nucleus of oocytes. The prelamin A is increased in both the nuclear membrane and nucleus, while phosphorylated lamin A (p-lamin A) is significantly reduced. AIPL1 was also proved to accumulate in the GV region of oocytes, and ZP3 deletion can significantly inhibit the aggregation of AIPL1 in the nuclear region. Similar to ZP3 knockdown, the absence of AIPL1 resulted in a decrease in the occurrence of GVBD, an increase in the amount of prelamin A, and a significant decrease in p-lamin A in oocytes developed in vitro. Finally, we propose the hypothesis that ZP3 can stabilize farnesylated prelamin A on the nuclear membrane of AIPL1, and promote its further processing into mature lamin A, therefore promoting the occurrence of GVBD. This study may be an important supplement for the mechanism of oocyte meiotic resumption and provide new diagnostic targets and treatment clues for infertility patients with oocyte maturation disorder.