Field studies were conducted at Delta Research and Extension Center, Stoneville, MS, in 1996, 1997, and 1998 to assess the effect of tillage systems (conventional tillage and subsoiling) on the environmental fate of imazaquin in a Sharkey clay soil. Imazaquin was applied preemergence at 140 g ai ha−1. Subsoiling in the fall did not affect imazaquin dissipation, total volume of runoff, imazaquin concentration in runoff, or imazaquin concentration in soil, as determined by chemical extraction. A corn root bioassay revealed no differences due to tillage systems in plant-available imazaquin in soil. Imazaquin concentration measured by chemical extraction or bioassay diminished over time, with a half-life ranging from 8 to 25 d. A field bioassay utilizing cotton and corn was conducted in 1997 and 1998 using plots that had received imazaquin the previous year. In 1997, 2 wk after planting, cotton and corn injury ranged from 3 to 15%, whereas no injury was observed in 1998. Injury symptoms declined over time, with no injury 5 wk after planting in either year. Although early-season cotton stunting and slight discoloration of corn was apparent in 1997, imazaquin residues did not affect subsequent vegetative and reproductive growing patterns of cotton or corn. In 1998, corn and cotton height were significantly greater in subsoiled plots compared to conventional tillage.