Linear instability analysis of a viscous swirling liquid jet surrounded by ambient gas is carried out by considering the significant influence of axial shear effect. The jet azimuthal flow is assumed as a Rankine vortex, and the non-uniform velocity distribution in the jet axial direction is approximated by parabolic and error functions. The enhancement of jet rotation is found to promote the predominant mode with larger azimuthal wavenumbers, and the mode transition is decided by the competition between centrifugal force and axial shear stress. Subsequently, the influence of the axial shear effect is examined through changing the degree of shear stress and the thickness of the gas velocity boundary layer. It is found that an increase of jet average velocity or surface velocity in the axial direction leads to the predominant mode transition to smaller azimuthal wavenumbers, due to the combined effects of shear stress and gas pressure perturbation. A larger velocity difference between ambient gas and liquid jet also promotes the predominant modes with smaller azimuthal wavenumbers, and the physical mechanism is attributed to gas pressure perturbation. Phase diagrams of different azimuthal modes are given and compared with the study of Kubitschek & Weidman (J. Fluid Mech., vol. 572, 2007, pp. 261–286), where a static swirling column without axial shear stress was considered. The strengthened axial shear effect is found to delay the transition of predominant modes with the increase of angular velocity. Experimental studies considering the swirling jets with different axial velocities are further carried out, which validate the theoretical findings. Different instability mechanisms and their transition rules are also identified through energy budget analysis. This study is expected to give scientific guidance on understanding the instability mechanisms of the swirling jets that widely exist in natural phenomena and engineering applications.

The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is the key vector insect transmitting the Candidatus Liberibacter asiaticus (CLas) bacterium that causes the devastating citrus greening disease (Huanglongbing, HLB) worldwide. The D. citri salivary glands (SG) exhibit an important barrier against the transmission of HLB pathogen. However, knowledge on the molecular mechanism of SG defence against CLas infection is still limited. In the present study, we compared the SG transcriptomic response of CLas-free and CLas-infected D. citri using an illumine paired-end RNA sequencing. In total of 861 differentially expressed genes (DEGs) in the SG upon CLas infection, including 202 upregulated DEGs and 659 downregulated DEGs were identified. Functional annotation analysis showed that most of the DEGs were associated with cellular processes, metabolic processes, and the immune response. Gene ontology and Kyoto Encyclopaedia of Genes and Genomes enrichment analyses revealed that these DEGs were enriched in pathways involving carbohydrate metabolism, amino acid metabolism, the immune system, the digestive system, the lysosome, and endocytosis. A total of 16 DEGs were randomly selected to further validate the accuracy of RNA-Seq dataset by reverse-transcription quantitative polymerase chain reaction. This study provides substantial transcriptomic information regarding the SG of D. citri in response to CLas infection, which may shed light on the molecular interaction between D. citri and CLas, and provides new ideas for the prevention and control of citrus psyllid.

To investigate the influence of inertia and slip on the instability of a liquid film on a fibre, a theoretical framework based on the axisymmetric Navier–Stokes equations is proposed via linear instability analysis. The model reveals that slip significantly enhances perturbation growth in viscous film flows, whereas it exerts minimal influence on flows dominated by inertia. Moreover, under no-slip boundary conditions, the dominant instability mode of thin films remains unaltered by inertia, closely aligning with predictions from a no-slip lubrication model. Conversely, when slip is introduced, the dominant wavenumber experiences a noticeable reduction as inertia decreases. This trend is captured by an introduced lubrication model with giant slip. Direct numerical simulations of the Navier–Stokes equations are then performed to further confirm the theoretical findings at the linear stage. For the nonlinear dynamics, no-slip simulations show complex vortical structures within films, driven by fluid inertia near surfaces. Additionally, in scenarios with weak inertia, a reduction in the volume of satellite droplets is observed due to slip, following a power-law relationship.

Given a graph G, the minimum Connected-k-Subgraph Cover problem (MinCkSC) is to find a minimum vertex subset C of G such that every connected subgraph of G on k vertices has at least one vertex in C. If furthermore the subgraph of G induced by C is connected, then the problem is denoted as MinCkSC$_{con}$. In this paper, we first present a PTAS for MinCkSC on an H-minor-free graph, where H is a graph with a constant number of vertices. Then, we design an $O((\omega+1)(2(k-1)(\omega+2))^{3\omega+3})|V|$-time FPT algorithm for MinCkSC$_{con}$ on a graph with treewidth $\omega$, based on which we further design an $O(2^{O(\sqrt{t}\log t)}|V|^{O(1)})$ time subexponential FPT algorithm for MinCkSC$_{con}$ on an H-minor-free graph, where t is an upper bound of solution size.

Student's t test is valid for statistical inference under the normality assumption or asymptotically. By contrast, although the bootstrap t test was proposed in 1993, it is seldom adopted in medical research. We aim to demonstrate that the bootstrap t test outperforms Student's t test under normality in data. Using random data samples from normal distributions, we evaluated the testing performance, in terms of true-positive rate (TPR) and false-positive rate and diagnostic abilities, in terms of the area under the curve (AUC), of the bootstrap t test and Student's t test. We explore the AUC of both tests with varying sample size and coefficient of variation. We compare the testing outcomes using the COVID-19 serial interval (SI) data in Shenzhen and Hong Kong, China, for demonstration. With fixed TPR, the bootstrap t test maintained the equivalent accuracy in TPR, but significantly improved the true-negative rate from the Student's t test. With varying TPR, the diagnostic ability of bootstrap t test outperformed or equivalently performed as Student's t test in terms of the AUC. The equivalent performances are possible but rarely occur in practice. We find that the bootstrap t test outperforms by successfully detecting the difference in COVID-19 SI, which is defined as the time interval between consecutive transmission generations, due to sex and non-pharmaceutical interventions against the Student's t test. We demonstrated that the bootstrap t test outperforms Student's t test, and it is recommended to replace Student's t test in medical data analysis regardless of sample size.

We investigate the impact of climate change on Gurenhekou glacier, southern Tibetan Plateau, which is representative of the tens of thousands of mountain glaciers in the region. We apply a three-dimensional, thermomechanically coupled full-Stokes model to simulate the evolution of the glacier. The steep and rugged bedrock geometry requires use of such a flow model. We parameterize the temperature and surface mass-balance (SMB) uncertainties using nearby automatic weather and meteorological stations, 6 year measured SMB data and an energy-balance model for a nearby glacier. Summer air temperature increased at 0.02 Ka−1 over the past 50 years, and the glacier has retreated at an average rate of 8.3 m a−1. Prognostic simulations suggest an accelerated annual average retreat rate of ~9.1 ma−1 along the central flowline for the next 25 years under continued steady warming. However, regional climate models suggest a marked increase in warming rate over Tibet during the 21st century, and this rate causes about a 0.9 ± 0.3% a−1 loss of glaciated area and 1.1 ± 0.6% a−1 shrinkage of glacier volume. These results, the rather high warming rates predicted and the small sizes of most Tibetan glaciers, suggest that significant numbers of glaciers will be lost in the region during the 21st century.

The Endangered giant panda Ailuropoda melanoleuca is one of the most threatened mammals. The species has experienced declines in its population and habitat as a result of human disturbance. We investigated the influence of human disturbance on habitat use by giant pandas in the Daxiangling Mountains, in China's Sichuan Province. We mapped all signs of giant panda and all locations of seven types of human disturbance in the study area. We used correlation analysis, generalized linear models, and Akaike information criteria to analyse the influence of the various types of human disturbances on habitat use by the giant panda. Our results showed that habitat use was positively correlated with elevation and distance from roads, residences, hydropower stations and logging or tree-felling sites, but negatively correlated with distance from bamboo shoot collection sites and trap sites. We found that the road-effect zone spanned a distance of c. 1,200 m and that human residence could affect the intensity of habitat use by giant pandas at distances > 2,500 m. The effect of roads on habitat use was probably influenced by the association of roads with residences, hydropower stations and mines. In the area occupied by giant pandas, we recommend increased regulation to minimize the expansion and impact of roads, residences, hydropower stations and logging activities.

We estimate all the individual glacier area and volume changes in High Mountain Asia (HMA) by 2050 based on Randolph Glacier Inventory (RGI) version 4.0, using different methods of assessing sensitivity to summer temperatures driven by a regional climate model and the IPCC A1B radiative forcing scenario. A large range of sea-level rise variation comes from varying equilibrium-line altitude (ELA) sensitivity to summer temperatures. This sensitivity and also the glacier mass-balance gradients with elevation have the largest coefficients of variability (amounting to ~50%) among factors examined. Prescribing ELA sensitivities from energy-balance models produces the highest sea-level rise (9.2 mm, or 0.76% of glacier volume a–1), while the ELA sensitivities estimated from summer temperatures at Chinese meteorological stations and also from 1°x1° gridded temperatures in the Berkeley Earth database produce 3.6 and 3.8 mm, respectively. Different choices of the initial ELA or summer precipitation lead to 15% uncertainties in modelled glacier volume loss. RGI version 4.0 produces 20% lower sea-level rise than version 2.0. More surface mass-balance observations, meteorological data from the glaciated areas, and detailed satellite altimetry data can provide better estimates of sea-level rise in the future.

The effect of the intercritical temperature on the microstructure and mechanical properties of a newly developed quenching and partitioning steel using martensitic microstructure prior to the heat treatment process was studied. Such a quenching and partitioning process possessed a unique microstructure evolution, especially during intercritical annealing after prequenching. Excellent mechanical properties were obtained due to this unique multiphase microstructure. Significant amount of interlath-retained austenite was acquired and the relationship between the microstructure and work-hardening behaviors was proposed. The martensite/austenite islands increased at elevated annealing temperature, which deteriorated the total elongation and increased the tensile strength as hard constituents when it was excessive. The result indicated that the present full martensitic microstructure before the intercritical annealing is probably more suitable to an industrial application and is a better way to produce high strength steels with suitable ductility.

In order to use two paralleled X-pinches as X-ray sources for the time-resolved backlighting of wire-array Z-pinch plasma, it is necessary to make these two X-pinches emit X-rays at different but roughly preset time instants. The timing of the X-ray burst from an X-pinch independence of the current, and the wire mass of the X-pinch was investigated. The currents flowing through two paralleled X-pinches were measured and it was found that the total current is almost equally divided between these two X-pinches no matter how different the wires for these two X-pinches are. The reason for the equal current division between two paralleled X-pinches was given based on the inductance calculation of the X-pinch circuit.

We conducted a retrospective study on non-typhoidal Salmonella isolates from patients with diarrhoea in Shanghai, China, 2006–2010. A total of 1484 isolates of 70 Salmonella serovars were recovered from about 18 000 stool specimens. Serovars Enteritidis and Typhimurium were the most prevalent with isolation rates of 27·6% and 25·5%, respectively. The majority (1151, 77·6%) of the isolates were resistant to at least one antimicrobial, and 598 (40·3%) to more than three antimicrobials. Approximately half (50·9%) of the isolates were resistant to nalidixic acid and other resistance rates were sulfisoxazole (47·9%), streptomycin (37·6%), ampicillin (31·3%) and tetracycline (30·5%). Co-resistance to fluoroquinolones and the third- and fourth-generation cephalosporins was also identified.

The purpose of this study was to determine the aetiology of acute diarrhoea and improve knowledge of gastrointestinal pathogens in China. Faecal specimens from 1526 outpatients with diarrhoea were collected from 20 hospitals in Henan province and the prevalence of seven bacterial and five viral diarrhoeagenic pathogens were determined. Bacterial pathogens were recovered from 279/1526 (18·3%) stool specimens and viral pathogens were detected in 178/1526 (11·7%) stool specimens. The top five pathogenic species were diarrhoeagenic E. coli (n = 140, 9·2%), rotavirus (n = 79, 5·2%), Shigella spp. (n = 69, 4·5%), Salmonella spp. (n = 66, 4·3%) and norovirus (n = 56, 3·7%). The prevalence of pathogens showed correlation with age, season and clinical symptoms. Several dominant serotypes were identified in Shigella and Salmonella isolates, and high prevalence of multiple drug-resistant isolates was observed in both species. This important information will have a significant effect on public health policy development and resource prioritization practices.

A high modulus, sulfonated polymer synthesized from one-to-one ratio 4,6-bis(4-hydroxyphenyl)-N, N-diphenyl-1,3,5-triazin-2-amine and 4,4′-biphenol with bis(4-fluorophenyl) sulfone (DPA-PS:BP) is exploited as an ionomer for micro-ionic actuators. A unique and attractive feature of the ionomer is that it can contain high amounts of ionic liquid (IL) as an electrolyte while maintaining a high elastic modulus (i.e 600 MPa for 150 wt% uptake), which is more than one order of magnitude higher than the state-of-the-art of ionomers with working electrolytes. Such a high modulus makes it possible for the ionomer to be fabricated into micro-actuators with high uptake of ILs and low operation voltage (< 4 V), in various free standing forms with ion milling techniques, which are attractive for MEMS applications. As an initial demonstration of a DPA-PS:BP based ionic micro-actuator, a cantilever (200 μm x 33 μm x 5 μm) is manufactured by Focused Ion Beam (FIB) and characterized. Under the voltage of 1.6 V, the bending actuator exhibits an intrinsic strain from the active ionomer of 1.1% and a corresponding blocking force of 27 μN.

Si80Ge20B0.6 thermoelectric alloys with minute erbium (Er) addition were prepared by the spark plasma sintering technique. The samples with different amounts of Er additions were analyzed by x-ray diffraction, x-ray fluorescence, and x-ray photoelectron spectroscopy. The thermoelectric properties were measured from 400 to 900 K. Effects of the amount of Er addition on the thermoelectric properties of Si80Ge20B0.6 alloys were investigated. New findings indicate that the Er-added alloys have larger carrier concentrations than the pristine sample. The larger carrier concentration appears to make a significant contribution to the electrical conductivity. Seebeck coefficient decreases with the increase of carrier concentration, whereas the power factor increases with increasing electrical conductivity. It was generally believed that the scattering of phonons by carriers may result in the thermal conductivity reduction. The samples with Er addition exhibit better figure of merits than the pristine sample. The optimal ratio of Er addition is actually in the range of 0.085–0.125 at.%.

The synthesis of Au and Ag metal nanoparticles stabilized by G4, G5, and G6 hyperbranched poly(amine–ester) (HPAE) is reported. The reduction of hydrogen tetrachloroaurate and silver nitrate in the presence of HPAE resulted in the formation of stable, uniform, water-soluble nanoparticles. The average particle sizes are (2.4 ±0.5)–(4.1 ±0.4) nm for Au and (2.5 ±4.9)–(10.3 ±1.7) nm for Ag, depending on the generation of HPAE and metal ion-to-end tertiary amine of HPAE (M:N) used. All of the obtained colloidal solutions are stable for a long period of time. The results from ultraviolet–visible absorption spectra and Fourier transform infrared absorption spectroscopic investigations confirm the interactions between metal and HPAE.