Synthetic auxin herbicides are commonly used in forage, pasture, range, and turfgrass settings for dicotyledonous weed control. Aminocyclopyrachlor (AMCP) is a newly developed pyrimidine carboxylic acid with a chemical structure and mode of action similar to the pyridine carboxylic acids—aminopyralid, clopyralid, and picloram. Injury to sensitive dicotyledonous plants has been observed following exposure to monocotyledonous plant material previously treated with pyridine compounds. The absorption, translocation, and metabolism of AMCP has been documented in susceptible broadleaf weeds; however, no information is available, to our knowledge, regarding AMCP fate in tolerant Poaceae, which may serve as the vector for off-target plant injury. Based on this premise, research was conducted to characterize absorption, translocation, and metabolism of AMCP in tall fescue. 14C-AMCP was applied to single tiller tall fescue plant foliage under controlled laboratory conditions at North Carolina State University (Raleigh, NC). Radiation was quantified in leaf wash, treated leaf, foliage, crown, roots, and root exudates at 3, 12, 24, 48, 96, and 192 h after treatment (HAT). 14C-AMCP was rapidly absorbed by tall fescue, reaching 38 and 68% at 3 and 48 HAT, respectively. Translocation of 14C-AMCP was limited to the foliage, which reached maximum translocation (34%) at 96 HAT. Most of the recovered 14C-AMCP remained in the leaf wash, treated leaf, or foliage, whereas minimal radiation was detected in the crown, roots, or root exudates throughout the 192-h period. No AMCP metabolism was observed in tall fescue through the 192 HAT. These data suggest AMCP applied to tall fescue can remain bioavailable, and mishandling treated plant material could result in off-target injury.