Historical changes from shifting land use, the natural meandering of waterways, and the aftereffects of erosion complicate modern environments and obfuscate precontact landscapes. Although archaeologists can create stratified sampling models or employ systematic surveys, traditional field methodologies are often not suitable for site discovery, thereby limiting knowledge of ancient cultural landscapes. Many water systems in southern Louisiana, and in many parts of the world, have been covered or concealed in backswamps by natural geomorphological processes, development, or environmental degradation. Investigation standards that do not account for these changes will not be effective at identifying archaeological sites in such transformed landscapes. Discoveries made during ongoing archaeological research in Iberville Parish, Louisiana, provide examples of what can be missed and offer solutions through changes in archaeological field methods. This article advocates for a mixed-methodology approach, drawing from historical research and shallow geophysics to look at landforms and landscape changes. Strictly following state survey guidelines can muddle the archaeological record, particularly in places subject to significant landscape change from historical land-use alteration. By applying these approaches, we offer a way to reconstruct ancient landscapes and landforms that are culturally significant but often missed given the nature of modern environmental conditions.

Dependencia theory is in vogue among social scientists throughout the world. Having originated in Latin America in the early 1960s, it was widely embraced both there and in parts of Europe and Africa by the end of the decade. In the 1970s, North Americans joined the flood of scholars attempting to grapple with the problems of underdevelopment in the so-called Third World from a perspective explicitly rejecting traditional theories of development. With this new stream of researchers came new tools and new approaches to the study of peripheral societies, tools and approaches intended to complement those previously used within the dependencia tradition.

OBJECTIVES/GOALS: Adolescents with congenital heart defects (CHD) have an elevated risk for future cardiovascular events, but information about their risk for e-cigarette use (“vaping”) is unknown. This study aims to present preliminary findings on gender differences in the association of impulsive behavior and vaping susceptibility from an ongoing investigation. METHODS/STUDY POPULATION: Adolescents with CHD (12-18 years; N=63) reported their vaping susceptibility and completed subjective (UPPS-P)/objective (Iowa Gambling Task; IGT) assessments of impulsive behavior previously associated with tobacco use. The UPPS-P includes 5 facets: 1) negative urgency (impulsivity under negative emotions), 2) positive urgency (impulsivity under positive emotions), 3) lack of premeditation (acting without thinking), 4) lack of perseverance (inability to focus), and 5) sensation seeking (seeking thrilling experiences). The IGT is a computerized task that creates conflict between immediate reward and delayed punishment via selections from advantageous/disadvantageous card decks. Linear regressions stratified by gender determined associations between vaping susceptibility and impulsivity. RESULTS/ANTICIPATED RESULTS: Nearly 30% (29.7%) of adolescents with CHD were susceptible to vaping. Negative urgency was associated with vaping susceptibility among females (β = 0.44, p = .035) but not males (β = 0.25, p = .128). Positive urgency was associated with vaping susceptibility among males (β = 0.37, p = .021) and trended toward significance among females (β = 0.40, p = .058). Lack of premeditation was associated with vaping susceptibility among males (β = 0.36, p = .025) but not females (β = 0.15, p = .490). The association between lack of perseverance and vaping susceptibility trended toward significance among males (β = 0.30, p = .064) but not females (β = -0.18, p = .413). IGT performance was not associated with susceptibility to vaping among either gender. UPPS-P facets and IGT performance were not significantly correlated. DISCUSSION/SIGNIFICANCE: The association of impulsivity and vaping susceptibility appears to be characterized by emotion-based rash action (positive/negative urgency) for females and by decreased conscientiousness (lack of premeditation/perseverance) for males. If replicated, the findings have implications for assessment of vaping risk and tailored intervention.

OBJECTIVES/SPECIFIC AIMS: The Clinical Research Operations Program is a free educational program designed to educate clinical research personnel on the conduct of clinical research (CR). The participant completes 16 required core sessions (24 h), 4 elective sessions (4 h), and passes the final exam to receive a certification in CR operations at Stanford. Sessions focus on the 9 domains of CR (established by the Joint Task Force for Clinical Trial Competency), such as Ethical & Participant Safety Considerations, Clinical Study Operations, & Data Management/Informatics. METHODS/STUDY POPULATION: Sessions are taught by volunteer lecturers. Participants may also attend the sessions without pursuing the certification. The program objective is to provide easy-access education in CR in order to increase regulatory compliance, staff retention, and improve CR at Stanford. The program targets CR coordinators, however, staff, postdocs, fellows, and faculty also participate. RESULTS/ANTICIPATED RESULTS: Since the program’s launch in January 2017, 119 individuals have enrolled in the certification program. The most represented group is the Department of Medicine. Sessions consistently reach their maximum with a waiting list. Each core session requires that the participant complete an evaluation (Likert scale, 1–5) of the registration process (4.5/5), the class environment (4.6/5), the presented content (4.5/5), and the instructor (4.6/5). Data from these evaluations are positive to date and is used to continually refine the program. DISCUSSION/SIGNIFICANCE OF IMPACT: N/A.

Evaluations of the effects of minimum tillage vs. conventional tillage and the effects of organic amendments (cover crops and compost) vs. no organic amendments were conducted in a California vegetable field. Weed densities were monitored, and soil samples were taken to measure the effects of the treatments on weed seedbanks and microbial biomass over a 24-mo period. Reduced tillage increased the density of shepherd's-purse in the upper soil layer (0 to 15 cm) of the soil seedbank compared with conventional tillage. Evidence is presented that suggests relationships between organic amendments, weed population reductions, and increases in soil microbial biomass: (1) shepherd's-purse emergence and seedbank densities were lower in the organic amendment plots, (2) microbial biomass was nearly always higher in the organic amendment plots, and (3) significant negative correlations between microbial biomass and burning nettle and shepherd's-purse emergence densities were found. These results suggest that organic matter addition may lead to reduced weed emergence.

Bird vetch is a perennial Eurasian plant which, unlike many exotic weed species, can invade low fertility areas that have not been disturbed. It also is common in pastures, woodland, and tall forb communities. Bird vetch is expanding along Alaskan roadsides, in urbanized areas, and in low density forests. A greenhouse study was conducted to determine efficacy of six herbicide treatments applied at reduced rates in 2005 and again in 2006 for bird vetch seedling control. Bird vetch seedlings were tolerant of reduced rates of chlorsulfuron and 2,4-DB; however, complete control was achieved with rates of clopyralid, dicamba plus diflufenzopyr, triclopyr, and 2,4-D that were a fourth to an eighth of the full registered rate. These results will be important for developing effective, low-cost methods for controlling bird vetch in Alaska, especially on the outer margins of infestations.

A single large colony (20 cm) of Rhombopora blakei n. sp. from the Hook Head Formation of Ireland (Tournaisian Stage, Mississippian) permits an analysis of within-colony variation associated with environmental change at a calendar scale (days to decades). Morphometric data for three external characters—apertures spacing along a branch and diagonal to a branch as well as lateral zooecial spacing—were collected as growth series (16–30 generations) from 13 segments within the colony. ANOVA, post-hoc means testing and graphical analysis of standardized data revealed nearest neighbor effects at the zooecial level and non-random distribution of variances across the colony. Parametric tests for sequential nonrandomness revealed cyclic variation through growth transects at three levels (23.3, 9.4 and 5.3 generations). Comparisons to growth rates of modern bryozoans suggests that the longer-term cycles are annual and that the shortest cycles may be related to lunar tidal cycles. The exceptional size and preservation of this single specimen, which is a new species of rhabdomesine Bryozoa, reinforces the importance of collecting individual morphological measurements from randomly selected and widely spaced parts of a colony for taxonomic, evolutionary and ecological applications.