The whitefly, Bemisia tabaci is a cryptic species complex in which one member, Middle East-Asia Minor 1 (MEAM1) has invaded globally. After invading large countries like Australia, China, and the USA, MEAM1 spread rapidly across each country. In contrast, our analysis of MEAM1 in India showed a very different pattern. Despite the detection of MEAM1 being contemporaneous with invasions in Australia, the USA, and China, MEAM1 has not spread widely and instead remains restricted to the southern regions. An assessment of Indian MEAM1 genetic diversity showed a level of diversity equivalent to that found in its presumed home range and significantly higher than that expected across the invaded range. The high level of diversity and restricted distribution raises the prospect that its home range extends into India. Similarly, while the levels of diversity in Australia and the USA conformed to that expected for the invaded range, China did not. It suggests that China may also be part of its home range. We also observed that diversity across the invaded range was primarily accounted for by a single haplotype, Hap1, which accounted for 79.8% of all records. It was only the invasion of Hap1 that enabled outbreaks to occur and MEAM1’s discovery.

Currently, there is an increasing demand for parallel mechanisms with fewer inputs and more outputs in aerospace, antennas, and recreational facilities. Therefore, this paper proposes a new parallel mechanism (PM) with a separation of constraints and drives, facilitating centralized drive management. Based on screw theory, the type synthesis of new parallel mechanisms with 3T (T representing translation), 3R (R representing revolute), 2T1R, and 2R1T is proposed. Three-degree-of-freedom (3-DOF) parallel mechanisms with fewer inputs and multiple outputs are proposed. Taking the 3RPS-PPPS parallel mechanism as an example, the closed-loop vector method is employed to solve for the lengths of actuated branches. A complete Jacobi matrix is constructed. The motion/force transmission performance of the 3RRS/PPPS parallel mechanism is better than the 3RRS parallel mechanism. Centralized management of drive branches greatly reduces the number of drives, making maintenance and repair easier. It has many application scenarios in antennas, recreational facilities, and other occasions.

MicroRNAs (miRNAs) are endogenous, non-coding RNAs, which are functional in a variety of biological processes through post-transcriptional regulation of gene expression. However, the role of miRNAs in the interaction between Bacillus thuringiensis and insects remains unclear. In this study, small RNA libraries were constructed for B. thuringiensis-infected (Bt) and uninfected (CK) Spodoptera exigua larvae (treated with double-distilled water) using Illumina sequencing. Utilising the miRDeep2 and Randfold, a total of 233 known and 726 novel miRNAs were identified, among which 16 up-regulated and 34 down-regulated differentially expressed (DE) miRNAs were identified compared to the CK. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that potential target genes of DE miRNAs were associated with ABC transporters, fatty acid metabolism and MAPK signalling pathway which are related to the development, reproduction and immunity. Moreover, two miRNA core genes, SeDicer1 and SeAgo1 were identified. The phylogenetic tree showed that lepidopteran Dicer1 clustered into one branch, with SeDicer1 in the position closest to Spodoptera litura Dicer1. A similar phylogenetic relationship was observed in the Ago1 protein. Expression of SeDicer1 increased at 72 h post infection (hpi) with B. thuringiensis; however, expression of SeDicer1 and SeAgo1 decreased at 96 hpi. The RNAi results showed that the knockdown of SeDicer1 directly caused the down-regulation of miRNAs and promoted the mortality of S. exigua infected by B. thuringiensis GS57. In conclusion, our study is crucial to understand the relationship between miRNAs and various biological processes caused by B. thuringiensis infection, and develop an integrated pest management strategy for S. exigua via miRNAs.

Bilinguals may choose to speak a language either at their own will or in response to an external demand, but the underlying neural mechanisms in the two contexts is poorly understood. In the present study, Chinese–English bilinguals named pairs of pictures in three conditions: during forced-switch, the naming language altered between pictures 1 and 2. During non-switch, the naming language used was the same. During free-naming, either the same or different languages were used at participants' own will. While behavioural switching costs were observed during free-naming and forced-switching, neuroimaging results showed that forced language selection (i.e., forced-switch and non-switch) is associated with left-lateralized frontal activations, which have been implicated in inhibitory control. Free language selection (i.e., free-naming), however, was associated with fronto-parietal activations, which have been implicated in self-initiated behaviours. These findings offer new insights into the neural differentiation of language control in forced and free language selection contexts.

In order to provide a theoretical foundation for the utilization of tailings as supplementary cementitious materials, the pozzolanic activity of muscovite—a typical mineral phase in tailings—before and after mechanical activation was investigated. In this study, significant pozzolanic activity of muscovite was obtained as a result of the structural and morphological changes that were induced by mechanical activation. The activated muscovite that was obtained after mechanical activation for 160 min satisfies the requirements for use as an active supplementary cementitious material, and the main characteristics of the pozzolana were as follows: median particle size (D50) of 11.7 μm, BET specific surface area of 28.82 m2 g−1, relative crystallinity of 14.99%, and pozzolanic activity index of 94.36%. Continuous grinding led to a gradual reduction in the relative crystallinity and an increase in the pozzolanic activity index due to the dehydroxylation reaction induced by mechanical activation, which occurred despite the fact that the specific surface area showed a decreasing trend when the grinding time was prolonged. Mechanically activated muscovite exhibited the capacity to react with calcium hydroxide to form calcium silicate hydrate, which is a typical characteristic of pozzolana. This experimental study provided a theoretical basis for evaluating the pozzolanic activity of muscovite using mechanical activation.

In order to establish a compact all-optical Thomson scattering source, experimental studies were conducted on the 45 TW Ti: sapphire laser facility. By including a steel wafer, mixed gas, and plasma mirror into a double-exit jet, several mechanisms, such as shock-assisted ionization injection, ionization injection, and driving laser reflection, were integrated into one source. So, the source of complexity was remarkably reduced. Electron bunches with central energy fluctuating from 90 to 160 MeV can be produced. Plasma mirrors were used to reflect the driving laser. The scattering of the reflected laser on the electron bunches led to the generation of X-ray photons. Through comparing the X-ray spots under different experimental conditions, it is confirmed that the X-ray photons are generated by Thomson scattering. For further application, the energy spectra and source size of the Thomson scattering source were measured. The unfolded spectrum contains a large amount of low-energy photons besides a peak near 67 keV. Through importing the electron energy spectrum into the Monte Carlo simulation code, the different contributions of the photons with small and large emitting angles can be used to explain the origin of the unfolded spectrum. The maximum photon energy extended to about 500 keV. The total photon production was 107/pulse. The FWHM source size was about 12 μm.

A transmission line circuit model was conducted to compare the performances of the two-level 2.5 Ω magnetically insulated transmission lines (MITLs) system of a 5-MA linear-transformer-driver (LTD) accelerator for two kinds of typical loads, including bremsstrahlung electron beam diodes and Z-pinch loads. Both the electron current loss in the pulse front during the magnetic insulation setup process and the electron flow distribution in the magnetic insulation steady state were analyzed. When the accelerator drives an electron beam diode load with impedance of 1.20 Ω (a single level), the duration of the magnetic insulation setup is about 12 ns, the current loss is about 130 kA in a single MITL level, the maximum electron flow current is about 50 kA in the end of MITL, and its amplitude decreases gradually after the steady magnetic insulation is established. When the accelerator drives a Z-pinch load with length of 1.5 cm, radius of 1.2 cm, and mass of 0.3 mg/cm, the duration of the magnetic insulation setup is almost zero, the maximum electron flow current in the end of MITL can reach about 55 kA (a single level), and the waveform of the electron flow resembles a saddle shape, which reaches the peak at the pinch stagnation time.

We conducted a retrospective, analytical cross-sectional and single-centre study that included 190 hospitalised COVID-19 patients in the Fujian Provincial Hospital South Branch between December 2022 and January 2023 to analyse the correlation of viral loads of throat swabs with clinical progression and outcomes. To normalise the Ct value as quantification of viral loads, we used RNase P gene as internal control gene and subtracted the Ct value of SARS-CoV-2 N gene from the Ct value of RNase P gene, termed △Ct. Most patients were discharged (84.2%), and only 10 (5.6%) individuals who had a lower △Ct value died. The initial △Ct value of participants was also significantly correlated with some abnormal laboratory characteristics, and the duration time of SARS-CoV-2 was longer in patients with severe symptoms and a lower △Ct value at admission. Our study suggested that the △Ct value may be used as a predictor of disease progression and outcomes in hospitalised COVID-19 patients.

Rhynchophorus ferrugineus is a quarantine pest that mainly damages plants in tropical regions, which are essential economic resources. Cry3Aa has been used to control coleopteran pests and is known to be toxic to R. ferrugineus. The binding of the Cry toxin to specific receptors on the target insect plays a crucial role in the toxicological mechanism of Cry toxins. However, in the case of R. ferrugineus, the nature and identity of the receptor proteins involved remain unknown. In the present study, pull-down assays and mass spectrometry were used to identify two proteins of aminopeptidase N proteins (RfAPN2a and RfAPN2b) in the larval midguts of R. ferrugineus. Cry3Aa was able to bind to RfAPN2a (Kd = 108.5 nM) and RfAPN2b (Kd = 68.2 nM), as well as midgut brush border membrane vesicles (Kd = 482.5 nM). In silico analysis of both RfAPN proteins included the signal peptide and anchored sites for glycosyl phosphatidyl inositol. In addition, RfAPN2a and RfAPN2b were expressed in the human embryonic kidney 293T cell line, and cytotoxicity assays showed that the transgenic cells were not susceptible to activated Cry3Aa. Our results show that RfAPN2a and RfAPN2b are Cry3Aa-binding proteins involved in the Cry3Aa toxicity of R. ferrugineus. This study deepens our understanding of the action mechanism of Cry3Aa in R. ferrugineus larvae.

The flow past a cylinder in proximity to a plane wall is investigated numerically for small gap ratios. Three vortex dynamic processes associated with different hairpin vortex generation mechanisms are identified for the first time, and the wake-induced turbulent transition is analysed. The vortex shedding is suppressed at $G/D = 0.1$, while the spanwise vortex is generated via a Kelvin–Helmholtz instability and evolves into hairpin vortices. For $G/D= 0.3$, the upper and lower rollers alternatively shedding from the cylinder, interact with the secondary vortex. The split secondary vortex merges with the upper roller and results in a new vortex downstream, which develops into hairpin vortices. When $G/D = 0.9$, the secondary vortex interacts with the lower roller and then evolves into hairpin vortices. A tertiary vortex induced by the secondary vortex is observed, rotating in the opposite direction to the secondary vortex the wake-induced transitions share the same route. The velocity fluctuations deviate from the optimal growth theory in the pre-transitional region. In the transitional region low-frequency disturbances penetrate the sheltering edge to generate streaks where the disturbance energy declines. In the turbulent region the logarithmic layer is formed, indicating that the turbulent equilibrium is established.

The Eastern Depression in the Bayanhaote Basin in western Inner Mongolia has experienced multi-stage Meso-Cenozoic tectonic events and possesses considerable exploration potential. However, structural deformation patterns, sequences and the genesis of oil-bearing structures in the basin are still poorly understood. In this study, based on high-quality 2D seismic data and drilling and well-logging data, we elucidate the activities and structural styles of faults, the tectonic evolution and the distribution characteristics of styles, as well as assessing potential petroleum traps in the Eastern Depression. Five types of faults that were active at different stages of the Meso-Cenozoic faults have been recognized: long-lived normal faults active since the late Middle Jurassic; reverse faults and strike-slip faults active in the late Late Jurassic; normal faults active in the Early Cretaceous; normal faults active in the Oligocene; and negative inverted faults active in the Early Cretaceous and Oligocene. These faults constituted 12 geometric styles in NE-trending belts at various stratigraphic levels, and were formed by compression, strike-slip, extension and inversion. The temporal development of structural styles promoted the formation and reconstruction and finalization of structural traps, while regional unconformities and open reverse and strike-slip faults provided migration pathways for petroleum to fill the traps. In general, potential traps that formed by compressional movement and strike-slip movement in the late Late Jurassic are primarily faulted anticlines. Those traps developed in Carboniferous rocks and are located in the southwestern region of the Eastern Depression, being controlled by NNE-NE-striking reverse and transpressive faults.

Almost all hospitals are equipped with air-conditioning systems to provide a comfortable environment for patients and staff. However, the accumulation of dust and moisture within these systems increases the risk of transmission of microbes and have on occasion been associated with outbreaks of infection. Nevertheless, the impact of air-conditioning on the transmission of microorganisms leading to infection remains largely uncertain. We conducted a scoping review to screen systematically the evidence for such an association in the face of the coronavirus disease 2019 epidemic. PubMed, Embase and Web of Science databases were explored for relevant studies addressing microbial contamination of the air, their transmission and association with infectious diseases. The review process yielded 21 publications, 17 of which were cross-sectional studies, three were cohort studies and one case−control study. Our analysis showed that, compared with naturally ventilated areas, microbial loads were significantly lower in air-conditioned areas, but the incidence of infections increased if not properly managed. The use of high-efficiency particulate air (HEPA) filtration not only decreased transmission of airborne bioaerosols and various microorganisms, but also reduced the risk of infections. By contrast, contaminated air-conditioning systems in hospital rooms were associated with a higher risk of patient infection. Cleaning and maintenance of such systems to recommended standards should be performed regularly and where appropriate, the installation of HEPA filters can effectively mitigate microbial contamination in the public areas of hospitals.