Cruznema velatum isolated from soil in a chestnut orchard located at Guangdong province, China, is redescribed with morphology, molecular barcoding sequences, and transcriptome data. The morphological comparison for C. velatum and six other valid species is provided. Phylogeny analysis suggests genus Cruznema is monophyletic. The species is amphimix, can be cultured with Escherichia coli in 7–9 days from egg to egg-laying adult, and has a lifespan of 11 to 14 days at 20°C. The transcription data generated 45,366 unigenes; 29.9%, 31.3%, 24.8%, and 18.6% of unigenes were annotated in KOG, SwissProt, GO, and KEGG, respectively. Further gene function analysis demonstrated that C. velatum share the same riboflavin, lipoic acid, and vitamin B6 metabolic pathways with Caenorhabditis elegans and Pristionchus pacificus.

An acute gastroenteritis (AGE) outbreak caused by a norovirus occurred at a hospital in Shanghai, China, was studied for molecular epidemiology, host susceptibility and serological roles. Rectal and environmental swabs, paired serum samples and saliva specimens were collected. Pathogens were detected by real-time polymerase chain reaction and DNA sequencing. Histo-blood group antigens (HBGA) phenotypes of saliva samples and their binding to norovirus protruding proteins were determined by enzyme-linked immunosorbent assay. The HBGA-binding interfaces and the surrounding region were analysed by the MegAlign program of DNAstar 7.1. Twenty-seven individuals in two care units were attacked with AGE at attack rates of 9.02 and 11.68%. Eighteen (78.2%) symptomatic and five (38.4%) asymptomatic individuals were GII.6/b norovirus positive. Saliva-based HBGA phenotyping showed that all symptomatic and asymptomatic cases belonged to A, B, AB or O secretors. Only four (16.7%) out of the 24 tested serum samples showed low blockade activity against HBGA-norovirus binding at the acute phase, whereas 11 (45.8%) samples at the convalescence stage showed seroconversion of such blockade. Specific blockade antibody in the population played an essential role in this norovirus epidemic. A wide HBGA-binding spectrum of GII.6 supports a need for continuous health attention and surveillance in different settings.

Superior and inferior rice grains have different weights and are located on the upper primary branch and lower secondary branches of the panicle, respectively. To study differences in germination vigour of these two types of grain, a number of factors were investigated from 0 to 48 h of germination. The present study demonstrated that in inferior grains the starch granule structure was looser at 0 h, with full water absorption at 48 h, while in superior grains the structure was tight and dense. Relative water content increased, and dry matter decreased, more rapidly in inferior grains than in superior ones. Abscisic acid and gibberellin levels, as well as α-amylase activity, also changed more rapidly in inferior grains, while soluble sugar content and amylase coding gene expression increased more rapidly in inferior than superior grains during early germination. The expression of OsGAMYB was higher in inferior grains at 24 h but higher in superior grains at 48 h. The phenotypic index of seedlings was higher in seedlings from superior grains at the two-leaf stage. However, the thousand-grain weight and yield per plant in superior and inferior plants showed no significant difference at harvest. The present study indicates that inferior grains germinate faster than superior ones in the early germination stage. Although inferior grains produced weaker seedlings, it is worthwhile using them in rice production due to their comparative yield potential over that of superior grains.

Phosphatidylinositol-3 kinases (PI3K)-Protein kinase B (Akt)-mammalian target of rapamycin (mTOR) pathway plays an important role in the synthesis and secretion of triacylglycerol. However, the mechanism of PI3K-Akt-mTOR pathway in regulating lipid metabolism of goose liver was poorly understood. The purpose of this study was to determine how PI3K-Akt-mTOR pathway regulating lipid metabolic homeostasis in goose hepatocytes. Goose primary hepatocytes were treated with different PI3K-Akt-mTOR signal inhibitors (LY294002, rapamycin and NVP-BEZ235) for 24 h. The results showed that these inhibitors evidently inhibited PI3K-Akt-mTOR downstream signaling. Meanwhile, these PI3K-Akt-mTOR inhibitors reduced intracellular lipid accumulation, decreased the mRNA expression and protein content of genes involved in the de novo fatty acid synthesis, while increased the transcriptional and protein level of key factors involved in fatty acid oxidation and very low density lipoprotein (VLDL) assembly and secretion. Conclusion: These findings suggested that the reduction of lipids accumulation induced-by inhibiting PI3K-Akt-mTOR pathway was closely linked to the decrease of lipogenesis, the increase of fatty acids oxidation, and the increase of VLDL assembly and secretion in goose hepatocytes.

The effects of laser incidence angle on lateral fast electron transport at front target surface, when a plasma is preformed, irradiated by intense (>1018 W/cm2) laser pulses, are studied by Kα imaging technique and electron spectrometer. A horizontally asymmetric Kα halo, resulting from directional lateral electron transport and energy deposition, is observed for a large incidence angle (70°). Moreover, a group of MeV high energy electrons is emitted along target surface. It is believed that the deformed preplasma and the asymmetrical distribution of self-generated magnetic field, at large incidence angle, play an important role in the directional lateral electron transport.

Site studies have proved Dome C to be an excellent site for optical interferometry, maybe the only possible site on Earth for the deployement of a large post-VLTI interferometric facility, like KEOPS. Indeed, recent studies have shown the possiblity of interferometric observations of faint objects, high sensitivity and high precision observations, direct imaging and nulling interferometry. However the achievement of this ambitious project assumes the pavement of a roadmap, including different steps. The first one should be a demonstrator, called Mykerinos. Mykerinos is a diluted aperture interferometer composed of at least two light collecting units with a baseline between 40 to 100 m (or more) consisting of  50 cm diameter telescopes. The telescope design is directly derived from our Laboratory's experience in the ASTEP experiment. Mykerinos will be equipped with a dual field focal instrument which is a pre-requisite for its site testing, as well as for its science demonstration purposes.

Runaway electron ream instabilities have been observed in Ohmic plasmas in the HT-7 tokamak. The instability regime is characterized by relaxations in the electron cyclotron emission due to the relativistic anomalous Doppler resonance effect which transfers energy from parallel to perpendicular motion. Two types of instabilities in the slide-away regime have been observed in the HT-7 tokamak. The scaling of the threshold value for the instabilities to occur has been derived. It is found that the threshold value is linearly dependent on the plasma current and independent of the toroidal magnetic field strength.

An experimental study on the angular distribution and conversion of multi-keV X-ray sources produced from 2 ns-duration 527nm laser irradiated thick-foil targets on Shenguang II laser facility (SG-II) is reported. The angular distributions measured in front of the targets can be fitted with the function of f(θ) = α+ (1−α)cosβθ (θ is the viewing angle relative to the target normal), where α = 0.41 ± 0.014, β = 0.77 ± 0.04 for Ti K-shell X-ray sources (~4.75 keV for Ti K-shell), and α = 0.085 ± 0.06, β = 0.59 ± 0.07 for Ag/Pd/Mo L-shell X-ray sources (2–2.8 keV for Mo L-shell, 2.8–3.5 keV for Pd L-shell, and 3–3.8 keV for Ag L-shell). The isotropy of the angular distribution of L-shell emission is worse than that of the K-shell emission at larger viewing angle (>70°), due to its larger optical depth (stronger self-absorption) in the cold plasma side lobe surrounding the central emission region, and in the central hot plasma region (emission region). There is no observable difference in the angular distributions of the L-shell X-ray emission among Ag, Pd, and Mo. The conversion efficiency of Ag/Pd/Mo L-shell X-ray sources is higher than that of the Ti K-shell X-ray sources, but the gain relative to the K-shell emission is not as high as that by using short pulse lasers. The conversion efficiency of the L-shell X-ray sources decreases with increasing atomic numbers (or X-ray photon energy), similar to the behavior of the K-shell X-ray source.

The space mission PEGASE, proposed to the CNES (Centre National d'Etudes Spatiales = French Space Agency) in the framework of its call for scientific proposals : “formation flying missions”, is a 2-aperture interferometer, composed by 3 free flying satellites (2 siderostats and 1 beam combiner), allowing baselines from 50 to 500 m in both nulling and visibility modes. With an angular resolution of a few mas and a spectral resolution of several tens in the spectral range 2.5-5 microns, PEGASE has several goals:

1. science : spectroscopy of hot jupiters (Pegasides) and brown dwarves, exploration of the inner part of protoplanetary disks

2. technology : validation in real space conditions of formation flying, nulling and visibility interferometry concepts.

The 1N/M7.6 flare of 24 October 2003 at S19E72 were well observed by RHESSI, TRACE, NORH, and the Spectrograph at the Purple Mountain Observatory. After analyzing all the data collected, we established a scenario of the flare and compared it with the standard cartoon of solar flares (e.g., Dennis 1988).To search for other articles by the author(s) go to: http://adsabs.harvard.edu/abstract_service.html

In a previous contribution, we implemented a finite locus model (FLM) for estimating additive and dominance genetic variances via a Bayesian method and a single-site Gibbs sampler. We observed a dependency of dominance variance estimates on locus number in the analysis FLM. Here, we extended the FLM to include two-locus epistasis, and implemented the analysis with two genotype samplers (Gibbs and descent graph) and three different priors for genetic effects (uniform and variable across loci, uniform and constant across loci, and normal). Phenotypic data were simulated for two pedigrees with 6300 and 12300 individuals in closed populations, using several different, non-additive genetic models. Replications of these data were analysed with FLMs differing in the number of loci. Simulation results indicate that the dependency of non-additive genetic variance estimates on locus number persisted in all implementation strategies we investigated. However, this dependency was considerably diminished with normal priors for genetic effects as compared with uniform priors (constant or variable across loci). Descent graph sampling of genotypes modestly improved variance components estimation compared with Gibbs sampling. Moreover, a larger pedigree produced considerably better variance components estimation, suggesting this dependency might originate from data insufficiency. As the FLM represents an appealing alternative to the infinitesimal model for genetic parameter estimation and for inclusion of polygenic background variation in QTL mapping analyses, further improvements are warranted and might be achieved via improvement of the sampler or treatment of the number of loci as an unknown.

Estimation of variance components with the finite polygenic model (FPM) was evaluated. Phenotypic data for a 6300-pedigree simulated under a wide range of additive genetic models were analysed with constant homozygote difference across loci using deterministic Maximum Likelihood (DML) and a Bayesian method implemented via Gibbs sampling (BGS). Results indicate that under no selection, both DML and BGS accurately estimated the variance components, with a FPM of 5 loci or more. When both analysis methods were applied to equivalent data sets on populations that had undergone selection, the DML method produced upward biased estimates of additive genetic variation and heritability due to its use of pedigree loop cutting, while BGS provided more accurate estimation. BGS was extended to non-additive FPMs with variable homozygote differences and dominance effect across loci. This method was used to analyse data simulated under two genetic models with positive, completely dominant gene action at all loci. Results indicate that the estimates of additive and dominance variances slowly increase as the number of loci in the FPM for analysis increases, while accuracy of predicting individual breeding values and dominance deviations remains unaffected. For the simulated pedigree structure, a FPM with 10 loci or slightly fewer appears to be appropriate for variance component estimation in the presence of dominance.

Copolymer latexes of poly(styrene/acrylic acid) were closely packed into two-dimensional arrays on glass, mica and silicon substrates respectively. When the small latexes were negatively charged due to −SO4− and −COO− groups on the surface, their film formation, in some degree, was affected by the electrostatic interaction between two particles as well as capillary force. Different electrolytes were added into the colloid to adjust the particle interaction so that the attractive and the repulsive forces were properly balanced and the ordered arrays were achieved. Thin metal film was deposited on the latex monolayer through thermal evaporation in vacuum. Then, the spheres were dissolved away to leave behind a surface with features located where the interstitial spaces between the densely packed spheres had been. This process depended upon the interaction between the microspheres and the substrates, and the adhesion between the film materials and the substrates. Different patterns of honeycomb net, triangle islands and separate spots can be obtained by controlling the ultrasonic time and intensity.

A two temperature finite difference model has been developed and is used to describe the response of materials under ultrafast femtosecond laser pulses in the energy regime where melting and vaporization can occur. In applying this model to silicon it is observed that, for 800 nm light, laser pulse intensities that are just sufficient to achieve threshold for vaporization are also at the level of optical electric field strength where electron avalanche breakdown at the surface of the material can occur. For sub-picosecond pulses the physical response of the material is associated with a strongly temperature dependent coupling coefficient connecting electron and phonon thermal distributions. The results of these analyses demonstrate that a very thin near solid density plasma, caused by avalanche ionization, is responsible for the surface heating and subsequent thermodynamic response of the material. This interpretation is consistent throughout the pulse duration range from 80 femtoseconds to 0.2 nanoseconds. The proposed mechanism for absorption, at the near infra-red wavelength being used here, is very different from the types of mechanisms usually considered for nanosecond laser heating of semiconductors. Surface damage threshold is determined by atomic force microscopy and the threshold for plasma optical emission by photomultiplier detection . Melt deths are probed with SIMS impurity diffusion profiles and high resolution cross sectional TEM.