With the widespread use of high-fat diets (HFDs) in aquaculture, the adverse effects of HFDs on farmed fish are becoming increasingly apparent. Creatine has shown potential as a green feed additive in farmed fish; however, the potential of dietary creatine to attenuate adverse effects caused by high fat diets remains poorly understood. To address such gaps, the present study was conducted to investigate the mitigating effect of dietary creatine on HFD-induced disturbance on growth performance, hepatic lipid metabolism, intestinal health, as well as muscle quality of juvenile largemouth bass. Three diets were formulated: a control diet (10.20% lipid), a high fat diet (HFD, 18.31% lipid), and HFD with 2% creatine (HFD + creatine). Juvenile largemouth bass (3.73±0.01g) were randomly assigned to three diets for 10 weeks. The key findings were: (1) The expression of muscle growth-related genes and proteins was stimulated by dietary creatine, which contribute to ameliorate the adverse effects of HFD on growth performance; (2) Dietary creatine alleviates HFD-induced adverse effects on intestinal health by improving intestinal health, which also enhances feed utilization efficiency; (3) Dietary creatine causes excessive lipid deposition, mainly via lipolysis and β-oxidation. Notably, the present study also reveal a previously undisclosed effect of creatine supplementation on improving muscle quality. Together, first time from a comprehensive multi-organ or tissue perspective, our study provides a feasible approach for developing appropriate nutritional strategies to alleviate the adverse effects of HFD on farmed fish, based on creatine supplementation.

Bovine mastitis harms milk quality and cattle health. Val-Pro-Pro (VPP) and Ile-Pro-Pro (IPP) are well-known milk-derived bioactive peptides with anti-inflammatory activity. However, the impact of VPP and IPP on mastitis remain unknown. This study aimed to investigate the anti-inflammatory effects and the underlying mechanisms of VPP and IPP in lipopolysaccharide (LPS)-induced inflammation. When cells were treated with LPS (1 µg/mL) for 24 h, the protein levels of pro-inflammatory factors (tumor necrosis factor-α (TNF-α), interleukin(IL)-1β and IL-6)) and chemokine (monocyte chemotactic protein-1 (MCP-1)) were markedly increased, and the protein level of anti-inflammatory cytokine (IL-10) was reduced. Both VPP and IPP with concentrations of 50 and 100 µM reversed these phenomena and further inhibited the protein expression of β-casein induced by LPS. In a mouse mastitis model, different concentrations of VPP and IPP (300, 600 µM/kg) pretreatment alleviated histopathological lesions in the mammary gland and suppressed the mRNA expression of TNFα, IL1β, and IL6 induced by LPS. VPP and IPP also maintained the integrity of the blood–milk barrier in mice. RNA-seq analyses indicated that enriched phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) and mitogen-activated protein kinase (MAPK) signaling pathways likely contribute to the changes observed (P < 0.05 and |log2 fold change (FC)| ≥ 1). Notably, fibronectin was identified as an important hub by protein–protein interaction (PPI) analysis and molecular docking combined with molecular dynamics simulation. In summary, VPP and IPP exerted a protective effect on LPS-induced inflammation by regulating PI3K/AKT signaling pathway via fibronectin.

The effect of the polarizations of two counter-propagating relativistic laser pulses interacting with subwavelength thin solid-density foil is investigated. Three-dimensional particle-in-cell simulations and analytical modelling show that the interaction and resulting transverse instability depend strongly on the polarization directions as well as the intensity distribution of the resultant light field in the foil. The left- and right-handed circularly polarized laser pair with the same phase at the common focal spot in the ultrathin foil leads to the strongest distortion of the foil. The fastest growing mode and maximum growth rate depend mainly on the laser intensity. For all polarization and phase-difference combinations, the instability is weakest when the two laser pulses are exactly out of phase at the common focusing point in the foil.

The body condition of cows during the early perinatal period has a long-term impact on the health of their offspring calves; however, research on the mechanisms of liver metabolism in this context is limited. This study investigates the effects of pre-perinatal cow body condition score (BCS) on various blood biochemical, antioxidant and immune indices in offspring calves. The calves were categorized into two groups based on their mothers’ BCS: the high body condition group (OHBCS) and the low body condition group (OLBCS). The results indicate that the levels of insulin, non-esterified fatty acid, β-hydroxybutyrate, immunoglobulin G, glutathione peroxidase and superoxide dismutase in the blood of the OHBCS group were significantly higher than those in the OLBCS group (p < 0.05). In contrast, serum levels of tumor necrosis factor-α and interleukin-2 in the OHBCS group were significantly lower than those in the OLBCS group (p < 0.001 and p < 0.05, respectively). Additionally, integrating metabolomic and transcriptomic data revealed that levels of the tricarboxylic acid cycle, fatty acid β-oxidation and ornithine cycling were reduced in the OHBCS group, whereas the ketogenic pathway and triglyceride synthesis pathway were enhanced. These findings elucidate the mechanisms by which pre-perinatal cow BCS influences liver metabolism in offspring calves.

A scheme for generating high-flux angularly uniform proton beams with high laser-to-proton energy conversion efficiency is proposed. Three laser beams are focused on a microwire array attached to a solid-density hemispheric target. The laser-driven hot electrons from the front of the microwire hemisphere generate a hot-electron sheath in the hollow behind it, so that the protons on its back are accelerated by target normal sheath acceleration. The accelerated protons are of high flux, as well as angularly and energetically uniform. The scheme should be useful for applications involving warm dense matter, such as isochoric heating and modification of materials, as well as for proton therapy and inertial confinement fusion.

Microorganisms symbiotic with insects, whether permanently or temporarily, play a crucial role in the nutrition, development, reproduction, defence, and metamorphosis regulation. In some Lepidoptera, oviposition-deterrent pheromones (ODPs) on egg surface were used by pregnant females to modify the behaviour of conspecifics to avoid excessive competition for limited resources. In this study, we constructed four different Spodoptera litura groups, including, OH, OA, SH, and OA, which either feed on different hosts or grow in different environments. The 16S rDNA libraries of microbes from the egg surface of the four groups were constructed and sequenced. According to alpha and beta diversity indices, the microbes in environments and diets considerably influenced the richness, diversity, and community compositions of the microbiota on egg surfaces. The quantity of the main ODP components and the corresponding oviposition-deterrent activity among four groups were significantly differed among the four groups. The result of this study revealed that altering of microbes in environments or diets considerably changed the contents of ODP and oviposition-deterrent activity. As ODPs impart oviposition-deterrent activity towards closely related species, the findings of this study suggest that we should pay more attention to the role of symbiotic microorganisms in changing the ability of insects, especially sympatric species, to occupy the optimal niche when developing novel pest-control strategies.

Isolated multi-MeV $\gamma$-rays with attosecond duration, high collimation and beam angular momentum (BAM) may find many interesting applications in nuclear physics, astrophysics, etc. Here, we propose a scheme to generate such $\gamma$-rays via nonlinear Thomson scattering of a rotating relativistic electron sheet driven by a few-cycle twisted laser pulse interacting with a micro-droplet target. Our model clarifies the laser intensity threshold and carrier-envelope phase effect on the generation of the isolated electron sheet. Three-dimensional numerical simulations demonstrate the $\gamma$-ray emission with 320 attoseconds duration and peak brilliance of $9.3\times 10^{24}$ photons s${}^{-1}$ mrad${}^{-2}$ mm${}^{-2}$ per 0.1$\%$ bandwidth at 4.3 MeV. The $\gamma$-ray beam carries a large BAM of $2.8 \times 10^{16}\mathrm{\hslash}$, which arises from the efficient BAM transfer from the rotating electron sheet, subsequently leading to a unique angular distribution. This work should promote the experimental investigation of nonlinear Thomson scattering of rotating electron sheets in large laser facilities.

The elasto-inertial focusing and rotating characteristics of spheroids in a square channel flow of Oldroyd-B viscoelastic fluids are studied by the direct forcing/fictitious domain method. The rotational behaviours, changes in the equilibrium positions and travel distances are explored to analyse the mechanisms of spheroid migration in viscoelastic fluids. Within the present simulated parameters (1 ≤ Re ≤ 100, 0 ≤ Wi ≤ 2, 0.4 ≤ α ≤3), the results show that there are four kinds of equilibrium positions and six (five) kinds of rotational behaviours for the elasto-inertial migration of prolate (oblate) spheroids. We are the first to identify a new rotational mode for the migration of prolate spheroids. Only when the particles are initially located at a corner and wall bisector, some special initial orientations of the spheroids have an impact on the final equilibrium position and rotational mode. In other general initial positions, the initial orientation of the spheroid has a negligible effect. A higher Weissenberg number means the faster the particles migrate to the equilibrium position. The spheroid gradually changes from the corner (CO), channel centreline (CC), diagonal line (DL) and cross-section midline (CSM) equilibrium positions as the elastic number decreases, depending on the aspect ratio, initial orientation and rotational behaviour of the particles and the elastic number of the fluid. When the elastic number is less than the critical value, the types of rotational modes of the spheroids are reduced. By controlling the elastic number near the critical value, spheroids with different aspect ratios can be efficiently separated.

Escherichia albertii is an emerging foodborne enteropathogen associated with infectious diarrhoea in humans. In February 2023, an outbreak of acute gastroenteric cases was reported in a junior high school located in Hangzhou, Zhejiang province, China. Twenty-two investigated patients presented diarrhoea (22/22, 100%), abdominal pain (21/22, 95.5%), nausea (6/22, 27.3%), and vomiting (3/22, 13.6%). E. albertii strains were successfully isolated from anal swabs collected from six patients. Each isolate was classified as sequence type ST2686, harboured eae-β gene, and carried both cdtB-I and cdtB-II subtypes, being serotyped as EAOg32:EAHg4 serotype. A comprehensive whole-genome phylogenetic analysis revealed that the six isolates formed a distinct cluster, separate from other strains. These isolates exhibited minimal genetic variation, differing from one another by 0 to 1 single nucleotide polymorphism, suggesting a common origin from a single clone. To the best of our knowledge, this represented the first reported outbreak of gastroenteritis attributed to E. albertii outside of Japan on a global scale.

This study exploits the correlation between Yellow River flooding and human capital accumulation using county-level data from Anhui, Hebei, Henan, Jiangsu, and Shandong provinces in China. Employing a spatial regression discontinuity approach, we compare the differences in human capital accumulation within and beyond the Yellow River floodplain areas (YRFA). Empirical results show human capital accumulation in the YRFA is at least 12.1 percent lower than outside the YRFA. Furthermore, our results demonstrate intergenerational transmission and gender differences in the negative impact of the Yellow River flooding on human capital accumulation. The baseline specifications of this study are not affected by drought and overall natural disasters. This paper documents historical human capital accumulation, clan power, and social trust, through which Yellow River flooding has a long-term impact.

Expectiles have received increasing attention as a risk measure in risk management because of their coherency and elicitability at the level $\alpha\geq1/2$. With a view to practical risk assessments, this paper delves into the worst-case expectile, where only partial information on the underlying distribution is available and there is no closed-form representation. We explore the asymptotic behavior of the worst-case expectile on two specified ambiguity sets: one is through the Wasserstein distance from a reference distribution and transforms this problem into a convex optimization problem via the well-known Kusuoka representation, and the other is induced by higher moment constraints. We obtain precise results in some special cases; nevertheless, there are no unified closed-form solutions. We aim to fully characterize the extreme behaviors; that is, we pursue an approximate solution as the level $\alpha $ tends to 1, which is aesthetically pleasing. As an application of our technique, we investigate the ambiguity set induced by higher moment conditions. Finally, we compare our worst-case expectile approach with a more conservative method based on stochastic order, which is referred to as ‘model aggregation’.

In the present paper, we study a new kind of anabelian phenomenon concerning the smooth pointed stable curves in positive characteristic. It shows that the topology of moduli spaces of curves can be understood from the viewpoint of anabelian geometry. We formulate some new anabelian-geometric conjectures concerning tame fundamental groups of curves over algebraically closed fields of characteristic $p>0$ from the point of view of moduli spaces. The conjectures are generalized versions of the Weak Isom-version of the Grothendieck conjecture for curves over algebraically closed fields of characteristic $p>0$ which was formulated by Tamagawa. Moreover, we prove that the conjectures hold for certain points lying in the moduli space of curves of genus $0$.

The geostationary orbit (GEO) belt hosts a substantial number of high-value satellites, making the study of autonomous navigation within this area significant. Features of autonomous operations such as patrolling the GEO belt and frequent manoeuvres at a certain location make real-time positioning using the Global Navigation Satellite System (GNSS) valuable. This paper studies the performance of positioning with GNSS considering main lobe and side lobe signals at different longitudes in the GEO belt. The research delves into the visibility and Position Dilution of Precision (PDOP) across the GEO belt, analysing the performance of the Global Positioning System (GPS), GLObalnaya NAvigatsionnaya Sputnikovaya Sistema (GLONASS) in Russian, BeiDou Navigation Satellite System (BDS), Galileo Navigation Satellite System (Galileo) and multi-systems. In particular, this paper investigates the impact of asymmetric constellations of mixed GEO, Inclined Geosynchronous Orbit (IGSO) and Medium Earth Orbit (MEO) satellites. The study reveals that BDS hybrid constellation provides long-term stable signal coverage over the GEO space above North America and Atlantic Ocean, where GEO signals are more sustainable while IGSO signals have wider coverage. This advantage positions BDS favourably in terms of performance in these regions.

Helicity plays a key role in the evolution of vortex structures and turbulent dynamics. The helicity dynamics and vortex structures in streamwise-rotating channel turbulence are discussed in this paper using the helicity budget equation and the differentiated second-order structure function equation of helicity. Generally, rotation and Reynolds numbers exhibit opposing effects on the interscale helicity dynamics and the vortices. Under the buffer layer, the positions of the helicity peaks are proportional to the ratio between the Reynolds and rotation numbers. The mechanism is related to the opposing effects of convection and rotation. Rotation directly affects the helicity balance through the Coriolis term and corresponding pressure term. In the buffer layer, the scale helicity is negative at small scales but positive at large scales, which is mainly induced by the spatial effects (the production and the spatial turbulent convection) but reduced by interscale cascades. Examination of structures reveals the close association between scale helicity and streaks, with streak lift angles exhibiting an increase with rotation and a decrease with Reynolds numbers. In the log-law layer, the Coriolis terms and corresponding pressure terms are proportional to the rotation numbers but remain independent of the Reynolds numbers. The negative scale helicity is forward cascaded towards small scales. Generally, spanwise vortices in the log-law layer are related to sweep events and forward cascades. Our findings indicate that these spanwise vortices are suppressed by rotation but recover with increasing Reynolds numbers, aligning with the effects observed in the scale helicity balance.

This study assessed the efficacy of ThinPrep cytologic test and human papillomavirus (HPV) co-test in cervical cancer screening during pregnancy. A cohort of 8,712 pregnant women from Ren Ji Hospital participated in the study. Among them, 601 (6.90%) tested positive for high-risk HPV (HR-HPV) and 38 (0.44%) exhibited abnormal cytology results (ASCUS+). Following positive HR-HPV findings, 423 patients underwent colposcopy, and 114 individuals suspected of having high-grade squamous intraepithelial lesion and cervical cancer (HSIL+) underwent cervical biopsy. Histological examination revealed 60 cases of normal pathology (52.63%), 35 cases of low‐grade squamous intraepithelial lesion (30.70%), 17 cases of HSIL (14.91%), and 2 cases of cervical cancer (1.75%). The incidence of HSIL+ in HPV 16/18 group was significantly higher than that in non-HPV16/18 group (10.53% vs. 6.14%, P < 0.05). Subsequent evaluation of the clinical performance of cytology alone, primary HPV screening, and co-testing for HSIL+ detection revealed that the HSIL+ detection rate was lowest with cytology alone. These findings suggest that HPV testing, either alone or combined with cytology, presents an efficient screening strategy for pregnant women, underscoring the potential for improved sensitivity in cervical cancer screening during pregnancy. The significantly higher incidence of HSIL+ in the HPV16/18 group emphasizes the importance of genotype-specific considerations.

In this paper the multiscale dynamics of streamwise-rotating channel turbulence is studied through direct numerical simulations. Using the generalized Kolmogorov equation, we find that as rotation becomes stronger, the turbulence in the buffer layer is obviously reduced by the intense spatial turbulent convection. On the contrary, in other layers, the turbulence is strengthened mainly by the modified production peak, the intense spatial turbulent convection and the suppressed forward energy cascades. It is also discovered that under a system rotation, small- and large-scale inclined structures have different angles with the streamwise direction, and the difference is strengthened with increasing rotation rates. The multiscale inclined structures are further confirmed quantitatively through a newly defined angle based on the velocity vector. Through the budget balance of Reynolds stresses and the hairpin vortex model, it is discovered that the Coriolis force and the pressure–velocity correlation are responsible for sustaining the inclined structures. The Coriolis force directly decreases the inclination angles but indirectly induces inclined structures in a more predominant way. The pressure–velocity correlation term is related to the strain rate tensor. Finally, the anisotropic generalized Kolmogorov equation is used to validate the above findings and reveals that the multiscale behaviours of the inclined structures are mainly induced by the mean spanwise velocity gradients.

Multigrain/polydispersity has a significant impact on turbidity current (TC). Despite the fact that several researches have looked into this effect, the impact of the fluid–particle interactions is not fully understood. Motivated by this, we employ the Eulerian–Lagrangian computational fluid dynamics–discrete element method model to investigate the dynamics of the bidisperse lock-exchange TC. Results show that, because the coarse particles will settle faster and stop moving forward, the two phases of bidisperse transport and fine component transport can be distinguished in the evolution of the bidisperse TC. During the bidisperse transport stage, the upper interface of each component is primarily determined by their own settling and transport characteristics and does not strongly depend on the relative fine particle volume fraction $\phi _F$. Fine particles are primarily responsible for the vortical structures near the upper interface of the TC head, and the increase of $\phi _F$ promotes their streamwise development. In comparison, fragmented vortical coherent structures are closely related to the presence of coarse particles, which can be seen in the lower layers. Bidisperse segregation alters the collision process between dispersed phases, which differs from monodisperse TC. The collisions and segregation-induced flow establish interconnections between the two dispersed phases. In the latter stage, the transport of fine particles is inhibited by both the lift force and the contact force produced by the collision with the deposited materials. As $\phi _F$ rises, the negative contact force weakens, and its change is essentially balanced by the rise in negative lift force.

Aging plays a crucial role in the mechanisms of the impacts of genetic and environmental factors on blood pressure and serum lipids. However, to our knowledge, how the influence of genetic and environmental factors on the correlation between blood pressure and serum lipids changes with age remains to be determined. In this study, data from the Chinese National Twin Registry (CNTR) were used. Resting blood pressure, including systolic and diastolic blood pressure (SBP and DBP), and fasting serum lipids, including total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and triglycerides (TGs) were measured in 2378 participants (1189 twin pairs). Univariate and bivariate structural equation models examined the genetic and environmental influences on blood pressure and serum lipids among three age groups. All phenotypes showed moderate to high heritability (0.37–0.59) and moderate unique environmental variance (0.30–0.44). The heritability of all phenotypes showed a decreasing trend with age. Among all phenotypes, SBP and DBP showed a significant monotonic decreasing trend. For phenotype-phenotype pairs, the phenotypic correlation (Rph) of each pair ranged from −0.04 to 0.23, and the additive genetic correlation (Ra) ranged from 0.00 to 0.36. For TC&SBP, TC&DBP, TG&SBP and TGs&DBP, both the Rph and Ra declined with age, and the Ra difference between the young group and the older adult group is statistically significant (p < .05). The unique environmental correlation (Re) of each pair did not follow any pattern with age and remained relatively stable with age. In summary, we observed that the heritability of blood pressure was affected by age. Moreover, blood pressure and serum lipids shared common genetic backgrounds, and age had an impact on the phenotypic correlation and genetic correlations.