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Effects of dietary conjugated linoleic acids on cellular immune response of piglets after cyclosporin A injection

Published online by Cambridge University Press:  22 April 2016

Y. X. Liu ,
K. Y. Zhu ,
Y. L. Liu  and
D. F. Jiang
Y. X. Liu*
Affiliation:
College of Animal Husbandry Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, Henan Province, P. R. China
K. Y. Zhu
Affiliation:
College of Animal Husbandry Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, Henan Province, P. R. China
Y. L. Liu
Affiliation:
Henan Engineering Technology Research Center of Animal Nutrition and Feed, Zhengzhou 450046, Henan Province, P. R. China
D. F. Jiang
Affiliation:
Henan Engineering Technology Research Center of Animal Nutrition and Feed, Zhengzhou 450046, Henan Province, P. R. China
*
†E-mail: yxliu225@163.com
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Abstract

The present study investigated the effects of dietary conjugated linoleic acid (CLA) on the cellular immune response of piglets after cyclosporin A (CsA) treatment. The experimental study had a 2×2 factorial design, and the main factors consisted of diets (0% or 2% CLA) and immunosuppression treatments (CsA or saline injection). CsA injection significantly increased feed : gain (F : G) of piglets (P<0.05); however, dietary CLA significantly decreased F : G of piglets (P<0.05). Dietary CLA partly ameliorated the deterioration of the feed conversion rate caused by CsA treatment (P<0.01). CsA treatment significantly decreased the percentages of CD4+ and CD8+ T lymphocytes in the thymus (P<0.01). Dietary CLA increased the percentages of CD4+ CD8+ double-positive and CD8+ single-positive T lymphocytes in the thymus (P<0.05), and had the trend to inhibit the decrease of CD4+ T lymphocytes in the thymus after CsA injection (P=0.07). CsA treatment significantly depleted the peripheral blood CD3+, CD4+ and CD8+ T lymphocytes (P<0.01). Dietary CLA significantly increased the number of peripheral blood CD8+ T lymphocytes and interleukin-2 (IL-2) production (P<0.05), and inhibited the decreases of peripheral blood CD3+, CD4+ and CD8+ T lymphocytes counts (P<0.01) as well as IL-2 production (P<0.05) after CsA treatment. Dietary CLA partly rescued the decrease of lymphocyte proliferation after CsA injection (P<0.05). In summary, dietary CLA effectively ameliorated CsA-induced cellular immunosuppression in piglets.

Keywords

conjugated linoleic acidspigletcyclosporin Acellular immunity
Type
Research Article
Information
animal , Volume 10 , Issue 10 , October 2016 , pp. 1660 - 1665
Copyright
© The Animal Consortium 2016 

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References

Bassaganya-Riera, J, Hontecillas, R, Zimmerman, DR and Wannemuehler, MJ 2001. Dietary conjugated linoleic acid modulates phenotype and effector functions of swine CD8+ lymphocytes. Journal of Nutrition 131, 2370–2377.CrossRefGoogle ScholarPubMed
Bassaganya-Riera, J, Hontecillas, R, Zimmerman, DR and Wannemuehler, MJ 2002. Long-term influence of lipid nutrition on the induction of CD8(+) responses to viral or bacterial antigens. Vaccine 20, 1435–1444.CrossRefGoogle ScholarPubMed
Bassaganya-Riera, J, Pogranichniy, RM, Jobgen, SC, Halbur, PG, Yoon, KJ, O’Shea, M, Mohede, IM and Hontecillas, R 2003. Conjugated linoleic acid ameliorates viral infectivity in a swine model of virally induced immunosuppression. Journal of Nutrition 133, 3204–3214.CrossRefGoogle Scholar
Blair, JT, Thomson, AW, Whiting, PH, Davidson, RJL and Simpson, JG 1982. Toxicity of the immune suppressant cyclosporine A in the rat. Pathology 138, 163–178.CrossRefGoogle Scholar
Eftekhari, MH, Aliasghari, F, Babaei-Beigi, MA and Hasanzadeh, J 2013. Effect of conjugated linoleic acid and omega-3 fatty acid supplementation on inflammatory and oxidative stress markers in atherosclerotic patients. ARYA Atherosclerosis 9, 311–318.Google Scholar
Fitzgerald, SD, Williams, SM and Reed, WM 1996. Development of a chicken model for studying avian polyomavirus infection. Avian Disease 40, 377–381.CrossRefGoogle ScholarPubMed
Furue, M and Ishibashi, Y 1991. Differential regulation by dexamethasone and cyclosporine of human T cells activated by various stimuli. Transplantation 52, 522–526.CrossRefGoogle Scholar
Lee, KN, Kritchevsky, D and Pariza, MW 1994. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108, 19–25.CrossRefGoogle ScholarPubMed
Hayek, MG, Han, SN, Wu, D, Watkins, BA, Meydani, M, Dorsey, JL, Smith, DE and Meydani, SN 1999. Dietary conjugated linoleic acid influences the immune response of young and old 57BL/6NCrlBR mice. Journal of Nutrition 129, 32–38.CrossRefGoogle Scholar
Hill, JE, Rowland, GN, Latimer, KS and Brown, J 1989. Effects of cyclosporine A on reovirus-infected broilers. Avian Disease 33, 86–92.CrossRefGoogle ScholarPubMed
Houseknecht, KL, Vanden-Heuvel, JP, Moya-Camarena, SY, Portocarrero, CP, Peck, LW, Nickel, KP and Belury, MA 1998. Dietary conjugated linoleic acid normalizes impaired glucose tolerance in the Zucker diabetic fatty fa/fa rat. Biochemical and Biophysical Research Communications 244, 678–682.CrossRefGoogle ScholarPubMed
Ip, C, Chin, SF, Scimeca, JA and Pariza, MW 1991. Mammary cancer prevention by conjugated dienoic derivative of linoleic acid. Cancer Research 51, 6118–6124.Google ScholarPubMed
Islam, MA, Kim, YS, Oh, TW, Kim, GS, Won, CK, Kim, HG, Choi, MS, Kim, JO and Ha, YL 2010. Superior anticarcinogenic activity of trans, trans-conjugated linoleic acid in N-methyl-N-nitrosourea-induced rat mammary tumorigenesis. Journal of Agricultural and. Food Chemistry 58, 5670–5678.CrossRefGoogle ScholarPubMed
Kelley, DS, Warren, JM, Simon, VA, Bartolinin, G, Mackey, BE and Erickson, EL 2002. Similar effects of c9, t11-CLA and t10-c12-CLA on immune cell functions in mice. Lipids 37, 725–728.CrossRefGoogle ScholarPubMed
Kim, D, Park, JH, Kweon, DJ and Han, GD 2013. Bioavailability of nanoemulsified conjugated linoleic acid for an antiobesity effect. International Journal of Nanomedicine 8, 451–459.Google ScholarPubMed
Lai, CH, Yin, JD, Li, DF, Zi, LD, Qiu, SY and Xu, JJ 2005. Conjugated linoleic acid attenuates the production and gene expression of proinflammatory cytokines in weaned piglets challenged with lipopolysaccharide. Journal of Nutrition 135, 39–44.Google Scholar
Lillehoj, HS 1987. Effects of immunosuppression on avian coccidiosis: cyclosporine A but not hormonal bursectomy abrogates host protective immunity. Infection and Immunity 55, 1616–1621.CrossRefGoogle Scholar
Long, FY, Guo, YM, Wang, Z, Liu, D, Zhang, BK and Yang, X 2011. Conjugated linoleic acids alleviate infectious bursal disease virus-induced immunosuppression in broiler chickens. Poultry Science 90, 1926–1933.CrossRefGoogle ScholarPubMed
Michael, ERD, Jennifer, LA and John, KGK 2004. Conjugated linoleic acid pork research. The American Journal of Clinical Nutrition 79, 1212S–1216S.Google Scholar
National Research Council 1998. Nutrient requirements of swine, 10th edition. National Academic Press, Washington, DC, USA.Google Scholar
Nugent, AP, Roche, HM, Noone, EJ, Long, A, Kelleher, DK and Gibney, MJ 2005. The effects of conjugated linoleic acid supplementation on immune function in healthy volunteers. European Journal of Clinical Nutrition 59, 742–750.CrossRefGoogle ScholarPubMed
O’Shea, M, Bassaganya-Riera, J and Mohede, ICM 2004. Immunomodulatory properties of conjugated linoleic acid. The American Journal of Clinical Nutrition 79 (suppl.), 1199S–1206S.CrossRefGoogle ScholarPubMed
Renner, L, Pappritz, J, Kramer, R, Kersten, S, Jahreis, G and Dänicke, S 2012. Fatty acid profile and proliferation of bovine blood mononuclear cells after conjugated linoleic acid supplementation. Lipids in Health and Disease 11, 63–69.CrossRefGoogle ScholarPubMed
Sigal, NH and Dumont, FJ 1992. Cyclosporine A, FK-506, and rapamycin: pharmacologic probes of lymphocyte signal transduction. Annual Review of Immunology 10, 519–560.CrossRefGoogle ScholarPubMed
Takahashi, K, Kawamata, K and Akiba, Y 2007. Effect of a mixture of conjugated linoleic acid (CLA) isomers on T cell subpopulation and responsiveness to mitogen in splenocytes of male broiler chicks. Asian-Australasian Journal of Animal Science 20, 954–961.CrossRefGoogle Scholar
Tanaka, M, Shinohara, K, Fukumoto, T, Tanaka, H and Kaneko, T 1988. Effect of cyclosporin A on rat thymus: time course analysis by immunoperoxidase technique and flow cytofluorometry. Clinical and Experimental Immunology 72, 216–221.Google ScholarPubMed
Wong, MW, Chew, BP, Wong, TS, Hosick, HL, Boylston, TD and Shultz, TD 1997. Effects of dietary conjugated linoleic acid on lymphocyte function and growth of mammary tumors in mice. Anticancer Research 17, 987–993.Google ScholarPubMed
Yamasaki, M, Chujo, H, Hirao, A, Koyanagi, N, Okamoto, T, Tojo, N, Oishi, A, Iwata, T, Yamauchi-Sato, Y, Yamamoto, T, Tsutsumi, K, Tachibana, H and Yamada, K 2003. Immunoglobulin and cytokine production from spleen lymphocytes is modulated in C57BL/6J mice by dietary cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid. Journal of Nutrition 133, 784–788.CrossRefGoogle ScholarPubMed
Zhang, HJ, Guo, Y and Yuan, J 2005. Conjugated linoleic acid enhanced the immune function in broiler chicks. British Journal of Nutrition 94, 746–752.CrossRefGoogle ScholarPubMed