Concurrent Evolution of Seed Dormancy and Herbicide Resistance in Field Populations of Dominant Weed Species in Western Australian Cropping Systems

Abstract Herbicide resistance, documented in many economically damaging weed species, is a major threat to global crop production. The injudicious use of herbicides, often in the absence of diverse weed control strategies, poses an immense selection pressure on weed communities for resistance evolution and weed adaptive traits such as high seed dormancy. This study evaluates the interaction among developing herbicide resistance, seed size, and seed dormancy of ripgut brome (Bromus diandrus Roth), wild oat (Avena fatua L.), and hare barley [Hordeum leporinum Link; syn. Hordeum murinum L. ssp. leporinum (Link) Arcang.] collected from within intensively managed fields (in-crop) in comparison with populations in surrounding ruderal (non-crop disturbed) areas with no history of exposure to herbicides within the Western Australian grainbelt. Seed size of the three species varied by farming system (continuous cereal-intensive annual crops, diverse annual crops, pasture based) and habitat (in-crop, ruderal). Field populations of H. leporinum and B. diandrus tended to have greater seed size compared with ruderal populations. Larger seeds had significantly more dormancy in all three weed species. Field-collected populations that were exposed to herbicide applications for at least the past 5 yr exhibited significantly greater seed dormancy compared with their counterparts present in ruderal areas within the same geographic area. The association between increased seed dormancy and developing multiple herbicide resistance further complicates effective weed management.


Introduction
Weed adaptation is an inevitable and damaging consequence of modern agricultural practices that involve high cultivation intensity of the same annual crops and overreliance on herbicides. The evolutionary adaptation of weeds has indirectly but largely been influenced by the process of crop domestication that has selected for weeds acclimatized to specific or diverse new ecosystems (Baucom 2019). The ecological fitness advantages of weeds, mainly driven by their genetic architecture, have enabled multiple adaptation strategies such as prolonged seed dormancy, rapid growth rates, ease of dispersal, stress tolerance, and herbicide resistance-collectively known as agricultural weed syndrome (Guo et al. 2018;Vigueira et al. 2013). Modern farming practices that heavily and persistently rely on herbicides under high cropping intensity have exerted selective forces on arable weeds, resulting in the aggravation of this syndrome and leading to widespread selection and evolution of herbicide-resistant individuals that can thrive in the managed environment (Beckie 2006;Murphy and Lemerle 2006;Owen et al. 2015a;Sun et al. 2021).
Apart from widespread herbicide resistance, multiple adaptive strategies have been reported to evolve in weed populations that are linked to various farming operations across agricultural systems. For example, hand weeding led to crop mimicry, reaping led to dwarf stature (reviewed in Barrett 1983), intensive cropping led to delayed germination (Kleemann and Gill 2006), repeated mowing led to prostrate growth habit (Warwick and Briggs 1979), and weed seed destruction may lead to early flowering and reduced podding height on the stem to evade capture at crop harvest (Ashworth et al. 2016). Moreover, weed adaptation in response to repetitive herbicide exposure has emerged as an unparalleled global threat to crop production. In contrast, the ruderal (non-crop disturbed) or natural areas surrounding intensively cultivated fields exhibit limited selection and evolution for herbicide resistance and life cycle or trait adaptations, indicating the prominent role of recurrent agronomic practices in directing the evolutionary patterns in weed adaptive traits (Owen et al. 2015a).
Since the 1990s, a number of studies have reported differential fitness costs in agricultural weeds that are resistant to one or multiple herbicides such as the inhibitors of acetyl CoA carboxylase (ACCase), acetolactate synthase (ALS), and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) (Maity et al. 2021a(Maity et al. , 2021bMartinez-Ghersa et al. 2000;Mortimer 1997). Various plant adaptive traits such as plant height, biomass, tiller number, leaf area index, regrowth rate, photosynthetic rate, and seed production potential have shown divergent responses in herbicide-resistant populations as opposed to their less intensively managed or non-cropland counterparts (Darmency et al. 2017;Gundel et al. 2008;Jasieniuk et al. 1996;Watkinson and White 1985). For example, Ghanizadeh and Harrington (2019) reported reduced plant height and plant biomass in triazine-resistant common lambsquarters (Chenopodium album L.) phenotypes as compared with a susceptible cohort. Henckes et al. (2019) reported that Italian ryegrass [Lolium perenne L. ssp. multiflorum (Lam.) Husnot] biotypes resistant to glyphosate, iodosulfuron, and pyroxsulam produced taller plants with increased shoot dry matter and absolute growth rate; these plants, however, had fewer tillers and reduced leaf area ratio when compared with the susceptible biotypes.
Seed size and dormancy are two important plant adaptive traits that have affected the colonization and persistence of weed species and plant evolution in general, especially under human-influenced plant domestication regimes (Rees 1996). Unsurprisingly, both of these seed traits have coadapted to different weed management practices along with other adaptation strategies such as widespread evolution of herbicide resistance (Kleemann and Gill 2006;Maity et al. 2021a;Owen et al. 2015a). Some studies failed to establish an association between altered weed seed traits and herbicide resistance (Harris et al. 1995;Holt and Thill 1994;Park et al. 2004;Wiederholt and Stoltenberg 1996). However, a number of studies have found that weed populations resistant to various herbicides exhibit alterations in their seed morphophysiological traits such as size, speed of germination, seed dormancy, and seedling vigor (Darmency et al. 2017;Ghersa et al. 1994;Maity et al. 2021a;Nandula et al. 2009;Owen et al. 2015aOwen et al. , 2015bYanniccari et al. 2016). These alterations may enhance the fitness of herbicide-resistant individuals. For example, altered seed dormancy can change the expected weed emergence patterns under field conditions, with cohorts evading control by soil-residual preemergence herbicides or being less controlled by postemergence herbicide applications.
Australian cropping systems, particularly in Western Australia (WA), have high adoption of no or minimum tillage (Llewellyn et al. 2012). Although conservation tillage has provided enormous benefits in improving soil properties, elimination of tillage as a weed control measure has resulted in overreliance on herbicides, leading to changes in weed dynamics and adaptation strategies (Chauhan et al. 2006;Owen et al. 2015a (Llewellyn et al. 2016). For example, Poole and Gill (1987) reported a 30% yield loss in wheat (Triticum aestivum L.) from Bromus spp. at densities of 100 plants m −2 in southern Australia; Dastheib et al. (2003) reported a 25% to 30% yield loss in wheat and barley (Hordeum vulgare L.) in New Zealand.
In general, seeds of Bromus spp. can remain substantially dormant for more than 2 mo (Kleeman and Gill 2006), seeds of H. leporinum for 3 mo (Bolger et al. 1999), and seeds of A. fatua for 3 to 4 mo (Paterson 1976) when stored at ambient room temperature after maturity. A number of studies in WA and other regions have confirmed the presence of field populations of these species resistant to one or multiple herbicides, and an association of seed traits, especially dormancy, with herbicide resistance (Owen and Powles 2016;Owen et al. 2011Owen et al. , 2015b. We hypothesize that intensive cropping practices have divergently influenced field populations of these species for seed trait expression and response to commonly used herbicides as compared with adjacent ruderal populations. In-crop weed populations with larger seed size and enhanced seed dormancy may have a fitness advantage (e.g., greater emergence, growth, fecundity) relative to their nonmanaged ruderal counterparts, which could exacerbate management efficacy. Therefore, the aim of this study was to investigate whether field and adjacent ruderal populations of B. diandrus, H. leporinum, and A. fatua differ in seed size and dormancy in relation to herbicide susceptibility.

Seed Collection
Weed populations were collected from different farms (two populations per farm: cropland and ruderal habitat) randomly selected across a variety of farming systems (continuous, diverse, and pasture) in different agroclimatic cropping zones within the WA grainbelt (Table 1). The continuous farming system comprised wheat and barley crops, whereas the diverse system comprised crop sequences of cereals (wheat, barley, and oat [Avena sativa L.]), canola (Brassica napus L.), and lupin (Lupinus angustifolius L.). Multiyear pastures (subterranean clover [Trifolium subterraneum L.]) in rotation with cereal, oilseed, and pulse crops listed above constituted the pasture farming system. Glyphosate and paraquat were applied as burndown herbicides before seeding. Soil-residual herbicides such as triallate, trifluralin, and prosulfocarb were frequently applied preemergence before seeding or at the time of seeding. Both ACCase-and ALS-inhibiting herbicides were commonly applied postemergence in the annual crops and pasture establishment year. For H. leporinum, 12 populations were from continuous (6 in-crop and 6 ruderal) and 36 from pasture (18 in-crop and 18 ruderal) farming systems. For B. diandrus, 30 populations were from diverse (15 in-crop and 15 ruderal) and 12 from pasture (6 in-crop and 6 ruderal) systems. For A. fatua, 6 populations each from continuous and pasture (3 incrop and 3 ruderal) and 12 populations from diverse (6 in-crop and 6 ruderal) systems were collected. Samples from mature seed heads for each population were collected from 40 to 50 plants randomly selected at each location (50 to 200 ha field area) during June 2018 before crop harvest. Collected seeds were threshed and cleaned from the chaff by aspiration, sieving, and forced-air separation.

Seed Size and Dormancy Evaluation
Immediately following collection, harvested seeds were placed in permeable paper bags and stored at ambient conditions (25 C in the dark). For each population, three replicate samples each comprising 100 seeds were weighed 12 mo after collection to assess relative seed size (mass), expressed as 100-seed weight (mg). A periodic evaluation of monthly (every 4 wk) seed dormancy release for 9 consecutive months after collection was performed using a standard test to assess germination. For each population, three replicates of 50 seeds each were subsampled every month. The subsampled seeds were sterilized in a 1:16 ratio of 12.5% sodium hypochlorite solution and then placed in 750-ml rectangular plastic trays (50 seeds per tray) containing 1% (10 g L −1 ) agar dissolved in deionized water. Trays were immediately transferred to a plant growth cabinet (Conviron® A1000; Conviron Australasia, Grovedale, Victoria 3216, Australia) that was set at environmental conditions conducive for seed germination: 12-h day at 20 C with white fluorescent light (30 to 60 μmol m −2 s −1 )/12-h night (dark) cycle at 11 C. Seed germination was evaluated daily for 4 wk after subsampling, with the criterion for germination being visible radicle protrusion (>1 mm) from the seed (Figure 1). Germinated seed was removed from the tray after each evaluation. At the end of the germination test, the remaining nongerminated seeds were assessed for viability by making a transverse section to expose the endosperm and incubating in 1% (10 g L −1 ) solution of 2,3,5-triphenyltetrazolium chloride for 24 h at 30 C. Evidence of pink staining of the embryo scored a seed as being viable; seeds with unstained embryos were considered nonviable (Verma and Majee 2013). Nonviable seeds were not included in the calculation of total seed count. Dormancy was calculated by the following formula (Equation 1): Dormancy % ð Þ ¼½ total viable seed count tðxÞ À germinated seed count gðxÞ À Á = total viable seed count tðxÞ Â 100 [1] where t(x) is the total viable seed count and g(x) is the total germinated seed count at evaluation time x, that is, after 4 wk from the start of a test.

Herbicide-Resistance Screening
Herbicide-resistance screening for B. diandrus and H. leporinum was conducted between June and August during the normal growing season in the Southern Hemisphere. Seeds of each population were germinated in 1% (10 g L −1 ) agar-water, as described earlier before being transplanted into plastic trays (40 cm by 30 cm by 10 cm) containing potting mix (50% composted pine bark, 25% peat, and 25% river sand). Plants were maintained outside with adequate light and soil moisture at the University of Western Australia until the 2-to 3-leaf stage. Fifty plants per herbicide for each population were sprayed with the upper range of the recommended dose of the herbicides as listed in Table 2, using a custom-built dual-nozzle (TeeJet® XR11001 flat fan, Springfield, IL, USA) cabinet sprayer (for details of spray application, see Owen et al. 2015b). Known herbicide-susceptible (100% mortality) and herbicide-resistant (<10% mortality) populations of each species were included as negative and positive controls, respectively, and the experiment was repeated. Herbicide efficacy was assessed by determining seedling mortality at 21 d after herbicide treatment (dead or alive). Flamprop-methyl was applied at the 4-leaf stage Samples from within the field that received common weed management practices for more than 5 yr Ruderal Samples from adjacent ruderal (noncropland) area of similar soil type located within 2 km of the corresponding in-crop sample site (e.g., roadsides or bushlands) and evaluated for mortality at 28 d after application. The proportion of survivors for each population for a particular herbicide was determined based on the number of plants that survived the herbicide application out of the total number of plants sprayed. The preemergence triallate treatment was applied to the soil surface, and 10 mm of untreated soil was immediately placed on the soil surface to prevent volatilization; mortality was assessed at 21 d after treatment. Although a single discriminating herbicide dose, based on previous dose-response experiments, is commonly used to classify weed populations collected in field surveys as herbicide resistant when both positive and negative controls are included (e.g., Owen et al. 2015aOwen et al. , 2015b, survivorship in this study denotes "developing herbicide resistance" (DHR) to conservatively indicate reduced sensitivity or potentially herbicide resistant.

Data Analysis
Experiments were conducted under controlled growth chamber (germination test) or net house conditions (herbicide screening) in a factorial randomized complete block design, with main factors being farming system (continuous, diverse, or pasture), weed population habitat (in-crop vs. ruderal), and weed species. Each farm was considered a replicated experimental site. Before statistical tests were conducted, data were checked for normality using the Shapiro-Wilk W test. No transformations were required. ANOVA (see Table 3 for response variables) and Pearson nonparametric correlation (ordinal variable) were performed using JMP v. 15.0 (SAS Institute, Cary, NC). Interactions of interest are detailed in Tables 4 and 5 and Figures 2 and 3 (significance denoted at P < 0.05, P < 0.01, or P < 0.001). In the few cases where standard errors were zero for a population (Table 5), that is, all replicates were equal to the population mean, no specific statistical tests could be conducted; the arithmetic difference between means was considered consistent and reportable in the absence of dispersion of the sample means. Statistical graphs were created using SigmaPlot v. 14.0 (Systat Software, San Jose, CA).
Time (months) required for 50% dormancy release (D 50 ) across the populations was estimated using a three-parameter sigmoidal model (Equation 2) on cumulative seed germination data collected over a 4-wk period. The model was constructed using SigmaPlot v. 14.0 and is described by Equation 2: where y is cumulative germination (%) on the xth day, a is maximum germination (%), x 0 is days to 50% germination, and b = slope at x 0 . Pearson correlation analysis was conducted in JMP PRO 14 to examine correlations between DHR to individual herbicides and seed size and dormancy. For this purpose, data on percent survival for each population in response to the different herbicides and percent dormancy (observed in the first-month evaluation) were used. Seed dormancy release rate can be influenced by numerous factors, including storage conditions. Percent seed dormancy represents a relatively consistent measurement of a population and hence is preferable to dormancy release rate for this correlation analysis. Due to the significant main effect of weed species on seed dormancy, seed size, and DHR, data are presented by species.

Results and Discussion
Seed dormancy of all three weed species differed among the two habitats assessed in this study (Table 3; Figure 2). Populations collected from within weed-managed fields (in-crop) exhibited greater seed dormancy compared with the corresponding population collected from an unmanaged ruderal location (Figure 2). At 7 mo after harvest, H. leporinum in-crop populations exhibited 52% to 100% seed dormancy compared with 17% to 24% for populations collected from the corresponding ruderal locations. After the same 7-mo duration, B. diandrus populations collected from in-crop locations had a dormancy range of 13% to 61% compared with 0% to 14% in the populations collected from ruderal locations. The A. fatua in-crop populations exhibited 5% to 49% seed dormancy compared with 4% to 17% dormancy in ruderal populations (P < 0.05). Results indicate that there was also an effect of farming system on seed dormancy (Table 3; Figure 2). Populations of H. leporinum, B. diandrus, and A. fatua from the farming systems with annual crops, whether continuous or diverse, exhibited greater seed dormancy compared with the pasture-intensive farming system. Rate of seed dormancy release was greatly impacted by both farming system and habitat (Table 4; Figure 2). In-crop populations released seed dormancy at a significantly slower rate than their ruderal counterparts, except for B. diandrus from the diverse system and H. leporinum from the pasture system. The time required for 50% dormancy release (D 50 ) for H. leporinum was 3.1 mo or more for in-crop populations compared with 2.8 to n = 15 n = 6 n = 6 AF 4.3 (0.21) A,b 2.7 (0.28) B,y 5.9 (0.14) A,a 3.4 (0.49) B,x 3.3 (0.11) A,c 2.7 (0.53) B,y n = 3 n = 3 n = 6 n = 6 n = 3 n = 3 a Symbols: ∞, seed dormancy did not release during the study period; -, the study did not include any population of the weed species from the specific farming system or habitat (n = no. of populations). Capital letters A and B indicate significant difference (P < 0.05) between weed habitats (in-crop vs. ruderal) for each weed species within a farming system; lowercase letters indicate significant difference for in-crop populations among farming systems (a, b, and c) and for ruderal populations (x and y).  indicates the study did not include any population of the weed species from the specific farming system or habitat. Significant difference between weed population habitat among farming systems at *P < 0.05 or **P < 0.01. Where SE is 0, arithmetic difference between habitats and/or farming systems was considered consistent and valid.
3.3 mo for ruderal populations. For B. diandrus, D 50 ranged from 2.6 to 4.7 mo for in-crop populations as compared with 1.5 to 2.3 mo for ruderal populations; D 50 of A. fatua ranged from 3.3 to 5.9 mo for in-crop populations and 2.7 to 3.4 mo for ruderal populations (Table 4). There was no consistent effect of farming system on dormancy release rate. Farming system had a significant influence on D 50 (Table 3), particularly for in-crop populations ( Table 4). The A. fatua in-crop populations from the pasture system released seed dormancy faster (D 50 of 3.3 mo) than the continuous (D 50 of 4.3 mo) and diverse systems (D 50 of 5.9 mo; P < 0.05). In contrast, B. diandrus in-crop populations from the diverse system released seed dormancy faster than in-crop populations from the pasture system (Table 4).
Seed size as inferred from 100-seed weight of the in-crop populations differed from their ruderal counterparts across farming systems. This study also found that farming system had a substantial influence on seed size (Table 3). In-crop B. diandrus populations had greater 100-seed weight than ruderal populations from the pasture and diverse systems (Figure 3). Additionally, the 100-seed weight of in-crop H. leporinum populations from the pasture system was 70% greater than for ruderal populations. The trend was consistent for B. diandrus, with 26% greater seed size for in-crop compared with ruderal populations from the same system. However, H. leporinum from the continuous system and A. fatua from all three systems showed the opposite trend. Under the continuous system, in-crop populations had lighter seeds than the ruderal populations for H. leporinum and A. fatua. Avena fatua from the pasture system showed a similar trend (P < 0.05; Figure 3). However, B. diandrus in-crop populations had greater seed weight than ruderal populations in the diverse Figure 2. Effect of farming system (continuous, diverse, or pasture) and weed population habitat (in-crop vs. ruderal) on monthly seed dormancy (means ± SE) release after harvest of (A) Hordeum leporinum, (B) Bromus diandrus, and (C) Avena fatua. Asterisks denote significant difference between means of weed population habitat within a specific farming system: *P < 0.05; **P < 0.01; ***P < 0.001.

314
Maity et al.: Weed dormancy and resistance farming system, but A. fatua in-crop populations had lighter seeds than their ruderal counterparts from the same system. Developing herbicide resistance, assessed as the survival of plants following herbicide exposure, varied significantly among species, farming systems, and habitats (Tables 3 and 5). Overall, all ruderal populations of the three species did not survive the field rate of these herbicides, except for <1% of plants of H. leporinum when treated with clethodim (Table 5). Hordeum leporinum in-crop populations from the continuous system had greater DHR to ALS inhibitors than populations from the pasture system: sulfometuron (96% vs. 33%, respectively) and sulfosulfuron (67% vs. 36%, respectively) (P < 0.05). Moreover, H. leporinum in-crop populations in the pasture system showed low levels of DHR to clethodim (4%) and fluazifop (15%).
Seed size, expressed as 100-seed weight, exhibited strong positive correlation with seed dormancy, irrespective of the farming system and habitat (Table 6). The correlation (r) was 0.79 for H. leporinum (P < 0.0001) and 0.85 for B. diandrus (P < 0.05). Avena fatua showed a low yet significant correlation (r = 0.37: P < 0.01) between seed size and dormancy. The seed size of B. diandrus was significantly correlated with sulfometuron DHR (r= 0.75; P < 0.05) (Table 6); however, this correlation was not evident for any other herbicides tested in this study (data not shown).
Populations with greater seed dormancy also exhibited greater DHR in the three weed species. This association was particularly evident for sulfometuron (r = 0.72, P < 0.01, and r = 0.83, P < 0.05, in H. leporinum and B. diandrus, respectively) and sulfosulfuron (r = 0.90, P < 0.01, and r = 0.85, P < 0.05, in H. leporinum and B. diandrus, respectively). This correlation was not significant for the other herbicides investigated for these two species (data not shown). A similar correlation between seed dormancy and DHR was identified in A. fatua populations for the ACCase inhibitor diclofop-methyl (r = 0.84, P < 0.01), but a weak correlation was seen with resistance to the ALS inhibitor mesosulfuron (r = 0.74, P < 0.09) ( Table 6).
This study found that H. leporinum, B. diandrus, and A. fatua present in intensively managed situations such as continuously cropped fields exhibit a higher level of seed dormancy compared with their counterparts from surrounding ruderal areas. Moreover, the annual crop populations of H. leporinum and B. diandrus tended to have greater seed size compared with ruderal populations. Several factors related to plant genetics as well as cropping practices might contribute to these differences observed between populations in cropped and non-cropped areas. While genotype plays a critical role in determining seed size and dormancy across plant species (Baskin and Baskin 2001;Fenner 1991), soil moisture stress and other stresses at any critical growth stage can alter or reduce seed size and can also reduce seed dormancy level, as determined in L. rigidum (Goggin et al. 2010;Steadman et al. 2004). Intensive cropping in semiarid (nonirrigated) arable land often leads to faster uptake of soil moisture by successive, early-maturing crops compared with natural grassland situations Niu et al. 2015), creating a relative drought condition for weed seed production. This situation may lead to increased seed dormancy or reduced seed germination, as reported by Cici (2017) in cowcockle [Vaccaria hispanica (Mill.) Rauschert]. Such a cropping practiceinduced survival strategy may decrease the risk of reproductive failure in weeds and increase their persistence in arable lands, especially under temporal environmental uncertainty.
We report that larger seeds had significantly more dormancy in all three weed species in the study. Gardarin and Colbach (2014) analyzed the seed size and dormancy in a number of weed species, including smooth pigweed (Amaranthus hybridus L.), and reported that larger seeds had higher levels of dormancy, in agreement with Grime et al. (1981), who analyzed more than 400 species collected in Sheffield, UK. Although Maity et al. (2021b) did not observe any strong correlation between L. perenne ssp. multiflorum seed size and dormancy, Rubio de Casas et al. (2017) and Chen et al. (2020) noted that plant species with large seeds usually exhibit reduced dormancy and can thrive in relatively unfavorable growing conditions. Their latter conclusion regarding stressful growing conditions agrees with findings in the present study in that the high dormant populations (correlated with large seed size) showed increased survivability to herbicide application, an imposed plant stress.
Larger seed size can promote increased vigor of the emerging seedlings leading to more robust plants in general (Bean 1972;Bebawi et al. 1984;Marcos-Filho 2015;Naylor and Jana 1976), which ultimately strengthens a weed's ability to persist in adverse environments (Bu et al. 2007;Buckley et al. 2003;Guillemin and Chauvel 2011). Weeds with greater seed mass or weight also facilitate range expansion potential, as reported by Daws et al. (2007) in a meta-analysis of 376 species and by Anderson (1990) investigating narrowleaf hawksbeard (Crepis tectorum L). Nurse and DiTommaso (2005) observed fewer velvetleaf (Abutilon theophrasti Medik) seeds produced in-crop under high corn (Zea mays L.) competition as compared with the field edges. Conditions leading to a reduction in the number of seeds often results in the production of larger seeds due to higher resource allocation per seed that may aid population persistence (Gallagher et al. 2013;Sawhney and Naylor 1982). Larger seeds promote faster growth, which may facilitate plants to develop to an advanced stage of growth that is less than optimum for effective herbicidal control. Significance level of the correlation between herbicide resistance or 100-seed weight and seed dormancy: *P < 0.05; **P < 0.01, ****P < 0.0001. c P < 0.09. d Correlation between B. diandrus survival (%) to sulfometuron and 100-seed weight was 0.75* (P < 0.05).
Consequently, the larger plants likely receive a lower dose than required for control, which can gradually lead to quantitative herbicide resistance evolution (Manalil et al. 2011). Although larger seed size was not generally associated with high DHR in this study, sulfometuron DHR in B. diandrus showed considerable positive correlation with seed size. Maity et al. (2021a) reported that L. perenne ssp. multiflorum seeds with larger size exhibited significantly greater resistance to multiple herbicides. Conducting additional studies with globally important weed species will provide further insights into this association.
There is a substantial level of DHR to multiple herbicide chemistries in all three weed species in this study. Widespread evolution of herbicide-resistant weed populations is now common in Australia for the major arable weeds, including Avena spp., Bromus spp., and Hordeum spp. (Broster et al. 2011;Heap 2021;Powles 2009, 2016). As ACCase and ALS inhibitors are the most frequently used herbicides to selectively control these weed species in a crop, resistance evolution to these high-risk herbicides is an inadvertent but expected outcome of their recurrent usage.
We found a direct and positive correlation between DHR (incrop habitat) and seed dormancy of populations of the three species in this study. This finding substantiates a widespread view that weed populations in intensive cropping systems exhibit greater seed dormancy than those populations inhabiting pasture or non-cropped areas (Gundel et al. 2008;Owen et al. 2011;Vila-Aiub et al. 2005). Owen et al. (2015aOwen et al. ( , 2015b reported a consistent trend of higher seed dormancy in L. rigidum and Bromus spp. populations from within intensively cropped fields that exhibited substantial resistance to different ACCase and ALS inhibitors. They postulated that the dormant cohort of a weed population escapes the pre-sowing weed management tools (preemergence herbicides or tillage) and thus augments the population with dormant individuals. These later-emerging individuals are exposed to repeated application of selective postemergence herbicides and eventually evolve resistance (Maity et al. 2021a). Similar phenomena were observed in Hordeum spp. in response to ALS-inhibiting and bipyridyl herbicides in WA (Owen et al. 2012;Yu et al. 2007), ACCase-inhibiting herbicides in southern Australia (Shergill et al. 2015), and ACCase-inhibiting and bipyridyl herbicides in eastern Australia (Heap 2021;Tucker and Powles 1991), and in A. fatua in response to herbicides of multiple sites of action including ALS inhibitors in Montana, USA (Lehnhoff et al. 2013) and WA (Owen and Powles 2016). However, these studies did not focus on quantifying the degree of correlation between the levels of seed dormancy and herbicide resistance.
The in-crop weed populations in this study were exposed to similar herbicide chemistries for at least 5 yr. Weed control measures are often applied repetitively at the same growth stage of the weeds, which may lead to a gradual but steady evolutionary shift to evade such measures (Sun et al. 2021). Co-occurrence of higher seed dormancy and DHR in the in-crop populations of H. leporinum, B. diandrus, and A. fatua tested in this study supports this assumption. However, coevolution or coexistence of herbicide resistance and seed dormancy observed in recent studies is a complex ecological phenomenon to decipher. Selection of resistance with recurrent herbicide use may not directly lead to an increase in seed dormancy (Gundel et al. 2008) or vice versa. Darmency et al. (2017) surmised that the linkage between seed dormancy and herbicide resistance may arise through selection of both traits simultaneously (pleiotropy). Two traits can arise simultaneously via separate mechanisms: the production by a single gene of two or more apparently unrelated effects (Gundel et al. 2008) or linkage between two separate genes conferring dormancy and resistance (Jordan 1999), though the latter is a rare process (Délye et al. 2013b). Délye et al. (2013a) postulated that singlelocus mutations (similar to target-site mutations) and complex multigenic stress responses (similar to non-target site mutations) in weeds may underlie the simultaneous evolution of multiple traits. In recent studies, single-locus resistance mutations have sometimes been linked to seed biochemical compositions that alter seed germination dynamics, such as fatty acids (Tremolières et al. 1988), abscisic acid (Topuz et al. 2015;Touraud et al. 1987), and branched-chain amino acids (Eberlein et al. 1999). However, ALS mutations are expected to promote faster germination because of lower abscisic acid content (Topuz et al. 2015), as abscisic acid is associated with higher dormancy (Kermode 2005). Yet evolution of weed populations with greater seed dormancy and tolerance to ALS-inhibiting herbicides, as evident from multiple recent studies including the present study, contradicts these assumptions. Much more research is required to understand the causal factors influencing this association. In general, epistatic interactions between alleles at different loci may impact the selection of weed traits that enhance fitness (Leon et al. 2021).
It has been clearly demonstrated that along with the widespread evolution of herbicide resistance, herbicide-dependent farming systems also select for weed life cycle traits that enable weeds to evade control practices. This study illustrates the ability of in-crop H. leporinum, B. diandrus, and A. fatua populations exposed to recurrent herbicide selection to escape early-season herbicide application through enhanced dormancy, as previously documented (Fleet and Gill 2012;Kleemann and Gill 2006;Owen et al. 2011). The combination of increased seed dormancy and herbicide resistance poses a tremendous ecological and agronomic challenge as populations with greater seed dormancy are able to evade the use of preseeding tillage or effective preemergence herbicide applications. These attributes can lead to increased usage of postemergence herbicides and limit the diversity of weed control tactics applied later in the crop season, which may lead to an increased selection intensity for herbicide resistance. In Australia, the net effect of prolonged weed seed dormancy on crop and weed growth and seed yield will depend on the duration of preemergence herbicide bioactivity in soil (e.g., pyroxasulfone greater than trifluralin), competitiveness of the crop species (e.g., cereals more competitive than pulses), period of the growing season (time required for crop canopy closure, emergence time differential between crop and dormant weeds, duration of crop growth advantage as soils cool going into winter), and timing of precipitation events.
While this study demonstrates that simultaneous evolution of both weed seed traits and DHR is occurring, such widespread adaptations need not be inevitable. The application of sufficient weed control diversity involving cultural, physical/mechanical, and herbicidal weed control tools within a crop-weed competitive environment has been demonstrated to reduce weed seedbanks to low and manageable levels (Sun et al. 2021;Walsh et al. 2017). Weed management diversity should help mitigate unidirectional selection pressures driving weed-trait adaptations. By minimizing weed seedbanks through maximizing crop competition, preharvest nonselective herbicide application (spray topping) when required, and harvest weed seed control (destroying or removing weed seeds in the chaff fraction of harvested crop residue), growers can limit the number of weeds under selection and thereby help mitigate further shifts in resistance evolution in response to herbicide exposure and physiological or phenological adaptations to evade weed control practices.