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Deep dehydration of Umbilicaria aprina thalli observed by proton NMR and sorption isotherm

Published online by Cambridge University Press:  09 June 2008

H. Harańczyk ,
M. Bacior  and
M.A. Olech
H. Harańczyk*
Affiliation:
Institute of Physics, Jagiellonian University, Cracow, Poland
M. Bacior
Affiliation:
Institute of Physics, Jagiellonian University, Cracow, Poland
M.A. Olech
Affiliation:
Institute of Botany, Jagiellonian University, Cracow, Poland Department of Antarctic Biology, Polish Academy of Sciences, Warsaw, Poland
*
*hubert.haranczyk@uj.edu.pl
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Abstract

The initial stages of Umbilicaria aprina Nyl. hydration (starting from the hydration level Δm/m0 = 0.048 ± 0.004) were observed using hydration kinetics, sorption isotherm and proton NMR. The thalli were hydrated from gaseous phase. The total saturation hydration level obtained at the relative humidity p/p0 = 100% was Δm/m0 = 0.848 ± 0.009. The hydration courses revealed i) a fraction of very tightly bound water (Δm/m0 = 0.054 ± 0.011, short hydration time constant, thyd), ii) a fraction of tightly bound water [Δm/m0 = 0.051 ± 0.038, thyd = (4.7 ± 2.6) h], and iii) a loosely bound water pool [thyd = (31.0 ± 1.9) h] for higher values of target humidity. The sorption isotherm of U. aprina was fitted well using Dent model. The relative mass of water saturating primary binding sites was ΔM/m0 = 0.054, which is close to the water fractions. Proton FIDs detected (i + ii) the immobilized tightly bound water fraction, L1, and iii) the mobile, loosely bound water pool L2. The hydration dependence of the proton liquid signal suggests the presence of a significant contribution from a water soluble solid fraction in the thallus. Sorption isotherm fitted to NMR data showed the absence of ‘sealed’ water fraction trapped in pores of the thallus.

Keywords

Antarcticalichensgenus Umbilicariadehydration resistance
Type
Biological Sciences
Information
Antarctic Science , Volume 20 , Issue 6 , December 2008 , pp. 527 - 535
Copyright
Copyright © Antarctic Science Ltd 2008

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