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Repeated acidosis challenges and live yeast supplementation shape rumen microbiota and fermentations and modulate inflammatory status in sheep

Published online by Cambridge University Press:  15 October 2013

M. Silberberg ,
F. Chaucheyras-Durand ,
L. Commun ,
M. M. Mialon ,
V. Monteils ,
P. Mosoni ,
D. P. Morgavi  and
C. Martin
M. Silberberg*
Affiliation:
INRA, UMR1213 Herbivores, Theix, F-63122 Saint-Genès Champanelle, France Université de Clermont-Ferrand, VetAgro Sup, UMR1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France
F. Chaucheyras-Durand
Affiliation:
Lallemand Animal Nutrition, Blagnac, France INRA, UR454 Microbiologie, Theix, F-63122 Saint-Genès Champanelle, France
L. Commun
Affiliation:
INRA, UMR1213 Herbivores, Theix, F-63122 Saint-Genès Champanelle, France VetAgro Sup, Campus vétérinaire de Lyon, F-69280 Marcy l’Etoile, France
M. M. Mialon
Affiliation:
INRA, UMR1213 Herbivores, Theix, F-63122 Saint-Genès Champanelle, France Université de Clermont-Ferrand, VetAgro Sup, UMR1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France
V. Monteils
Affiliation:
INRA, UMR1213 Herbivores, Theix, F-63122 Saint-Genès Champanelle, France Université de Clermont-Ferrand, VetAgro Sup, UMR1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France Université de Toulouse, INPT-ENSAT, UMR1289 Tissus Animaux Nutrition Digestion Ecosystème et Métabolisme, F-31326 Castanet-Tolosan, France INRA, UMR1289, Tissus Animaux Nutrition Digestion Ecosystème et Métabolisme, F-31326 Castanet-Tolosan, France Université de Toulouse, INPT-ENVT, UMR1289 Tissus Animaux Nutrition Digestion Ecosystème et Métabolisme, F-31076 Toulouse, France
P. Mosoni
Affiliation:
INRA, UR454 Microbiologie, Theix, F-63122 Saint-Genès Champanelle, France
D. P. Morgavi
Affiliation:
INRA, UMR1213 Herbivores, Theix, F-63122 Saint-Genès Champanelle, France Université de Clermont-Ferrand, VetAgro Sup, UMR1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France
C. Martin
Affiliation:
INRA, UMR1213 Herbivores, Theix, F-63122 Saint-Genès Champanelle, France Université de Clermont-Ferrand, VetAgro Sup, UMR1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France
*
†E-mail: mathieu.silberberg@clermont.inra.fr
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Abstract

This study aimed to investigate the impact of repeated acidosis challenges (ACs) and the effect of live yeast supplementation (Saccharomyces cerevisiae I-1077, SC) on rumen fermentation, microbial ecosystem and inflammatory response. The experimental design involved two groups (SC, n=6; Control, n=6) of rumen fistulated wethers that were successively exposed to three ACs of 5 days each, preceded and followed by resting periods (RPs) of 23 days. AC diets consisted of 60% wheat-based concentrate and 40% hay, whereas RPs diets consisted of 20% concentrate and 80% hay. ACs induced changes in rumen fermentative parameters (pH, lactate and volatile fatty-acid concentrations and proportions) as well as in microbiota composition and diversity. The first challenge drove the fermentation pattern towards propionate. During successive challenges, rumen pH measures worsened in the control group and the fermentation profile was characterised by a higher butyrate proportion and changes in the microbiota. The first AC induced a strong release of rumen histamine and lipopolysaccharide that triggered the increase of acute-phase proteins in the plasma. This inflammatory status was maintained during all AC repetitions. Our study suggests that the response of sheep to an acidosis diet is greatly influenced by the feeding history of individuals. In live yeast-supplemented animals, the first AC was as drastic as in control sheep. However, during subsequent challenges, yeast supplementation contributed to stabilise fermentative parameters, promoted protozoal numbers and decreased lactate producing bacteria. At the systemic level, yeast helped normalising the inflammatory status of the animals.

Keywords

sheeprumen acidosisrumen microbiotarumen fermentationsinflammatory status
Type
Nutrition
Information
animal , Volume 7 , Issue 12 , December 2013 , pp. 1910 - 1920
Copyright
Copyright © The Animal Consortium 2013 

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Footnotes

*

Both authors contributed equally to the present work.

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