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Complete sequence of the Tibetan Mastiff mitochondrial genome and its phylogenetic relationship with other Canids (Canis, Canidae)

Published online by Cambridge University Press:  16 July 2010

Yinxia Li ,
Qifa Li ,
Xingbo Zhao ,
Zhuang Xie  and
Yinxue Xu
Yinxia Li
Affiliation:
Laboratory of Animal Reproductive Genetics and Molecular Evolution, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Qifa Li*
Affiliation:
Laboratory of Animal Reproductive Genetics and Molecular Evolution, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Xingbo Zhao
Affiliation:
State Key Laboratory for Agribiotechnology, College of Life Science, China Agricultural University, Beijing 100094, China
Zhuang Xie
Affiliation:
Laboratory of Animal Reproductive Genetics and Molecular Evolution, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Yinxue Xu
Affiliation:
Laboratory of Animal Reproductive Genetics and Molecular Evolution, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
*
† E-mail: liqifa@njau.edu.cn
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Abstract

In this study, the complete sequence of the Tibetan Mastiff mitochondrial genome (mtDNA) was determined, and the phylogenetic relationships between the Tibetan Mastiff and other species of Canidae were analyzed using the coyote (Canis latrans) as an outgroup. The complete nucleotide sequence of the Tibetan Mastiff mtDNA was 16 710 bp, and included 22 tRNA genes, 2S rRNA gene, 13 protein-coding genes and one non-coding region (D-loop region), which is similar to other mammalian mitochondrial genomes. The characteristics of the protein-coding genes, non-coding region, tRNA and rRNA genes among Canidae were analyzed in detail. Neighbor-joining and maximum-parsimony trees of Canids constructed using 12 mitochondrial protein-coding genes showed that as the coyotes and Tibetan wolves clustered together, so too did the gray wolves and domestic dogs, suggesting that the Tibetan Mastiff originated from the gray wolf as did other domestic dogs. Domestic dogs clustered into four clades, implying at least four maternal origins (A to D). The Tibetan Mastiff, which belongs to clade A, appears to be closely related to the Saint Bernard and the Old English Sheepdog.

Keywords

Tibetan MastiffCanidsmitochondrial genomephylogenetics
Type
Full Paper
Information
animal , Volume 5 , Issue 1 , January 2011 , pp. 18 - 25
Copyright
Copyright © The Animal Consortium 2010

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