Patterns and predictors of alcohol misuse trajectories from adolescence through early midlife

We took a multilevel developmental contextual approach and characterized trajectories of alcohol misuse from adolescence through early midlife, examined genetic and environmental contributions to individual differences in those trajectories, and identified adolescent and young adult factors associated with change in alcohol misuse. Data were from two longitudinal population-based studies. FinnTwin16 is a study of Finnish twins assessed at 16, 17, 18, 25, and 35 years ( N = 5659; 52% female; 32% monozygotic). The National Longitudinal Study of Adolescent to Adult Health (Add Health) is a study of adolescents from the United States, who were assessed at five time points from 1994 to 2018 ( N = 18026; 50% female; 64% White, 21% Black, 4% Native American, 7% Asian, 9% Other race/ethnicity). Alcohol misuse was measured as frequency of intoxication in FinnTwin16 and frequency of binge drinking in Add Health. In both samples, trajectories of alcohol misuse were best described by a quadratic growth curve: Alcohol misuse increased across adolescence, peaked in young adulthood, and declined into early midlife. Individual differences in these trajectories were primarily explained by environmental factors. Several adolescent and young adult correlates were related to the course of alcohol misuse, including other substance use, physical and mental health, and parenthood.

The vast majority of longitudinal studies on the development of alcohol misuse focus on adolescence through young adulthood, perhaps driven by the idea that many individuals "mature out" of binge drinking during the transition to adulthood (Lee & Sher, 2018).Nonetheless, alcohol misuse remains common in early midlife, with 32% of individuals in their 30s and 40s reporting pastmonth binge drinking (Substance Abuse and Mental Health Services Administration, 2018).Further, middle-aged Americans have uniquely experienced an increase in all-cause mortality over the past 20 years.Rising mortality rates in this age group are primarily driven by suicide, drug and alcohol poisoning, and chronic liver diseases and cirrhosis (Case & Deaton, 2015), all of which are directly or indirectly linked to alcohol misuse (Brady, 2006;Rehm et al., 2010).Therefore, delineating the nature and predictors of alcohol misuse trajectories through early midlife may provide critical insight into morbidity and mortality and highlight targets for prevention and intervention efforts.
Previous efforts to describe trajectories of alcohol misuse through early midlife have primarily relied upon person-centered approaches (Berg et al., 2013(Berg et al., , 2019;;Jackson & Sher, 2005;Vachon et al., 2017;Virtanen et al., 2015), which classify individuals into groups based on their patterns of change in alcohol misuse (Jung & Wickrama, 2008).These methods are appealing because they may identify distinct subpopulations of individuals at particular risk for alcohol problems (Muthén & Muthén, 2000).However, personbased trajectory approaches nearly always yield four groups, which are characterized by consistently low levels of alcohol involvement, increasing alcohol involvement across the study period, decreasing alcohol involvement across the study period, and consistently high levels of alcohol involvement.Prior work suggests that identification of these four typologies may be driven by the analytic approach, rather than meaningful variation in alcohol misuse (Sher et al., 2011).Therefore, variable-centered approaches, which focus on the relationships among variables rather than the relationships among individuals, are also needed to characterize developmental trajectories of alcohol misuse.
Several studies have adopted a variable-centered approach to describe trajectories of alcohol consumption through early midlife, showing that frequency and quantity of alcohol use generally increase across adolescence, peak in the early or mid-20s, and decline thereafter (Britton et al., 2015;Zellers et al., 2022).Nonetheless, conducting variable-centered analyses of developmental change in indices of alcohol misuse (e.g., heavy drinking, binge drinking) remains an important next step.In the present study, we applied a variable-centered approachmultilevel growth modelingto investigate trajectories of alcohol misuse from adolescence through early midlife in two population-based samples.In addition, we adopted a multilevel developmental contextual approach to understand individual differences in patterns in alcohol misuse over time.
A multilevel developmental contextual approach to alcohol misuse Windle's multilevel developmental contextual framework proposes that substance use and problems are dynamic processes influenced by a comprehensive set of multilevel contextual factors, including person-level factors (e.g., genetic liability, personality characteristics), proximal environmental factors (e.g., features of the parent-child relationship, marital conflict), distal environmental factors (e.g., media portrayals of substance use), and related emotional, behavioral, and health problems.This framework also recognizes that person-level and environmental factors influence one another in a bidirectional manner, and the relationships between risk factors vary across development (Windle, 2010).
Consistent with the multilevel developmental contextual framework, there is evidence to suggest that genetic, person-level, and environmental factors are related to patterns of alcohol misuse across development.There is a substantial genetic component to alcohol-related outcomes (Verhulst et al., 2015), and person-level psychological factors, such as personality traits and neurocognitive functioning, have also been associated with alcohol misuse (Courtney & Polich, 2009;Kotov et al., 2010;Sher et al., 1997;Stautz & Cooper, 2013).Further, environmental factors play an important role in the development of alcohol use and problems, though the relevance of specific environmental factors varies based on developmental stage.For example, parenting behaviors and peer substance use are strongly associated with patterns of alcohol misuse in adolescence (Leung et al., 2014;Patrick & Schulenberg, 2014;Steinberg et al., 1994), whereas romantic relationship status and employment assume increasing importance in young adulthood (Boden et al., 2017;Fischer & Wiersma, 2012;Lee et al., 2015).

Predictors of alcohol misuse in the 30s and 40s
Early midlife remains an understudied period, but several crosssectional and prospective studies have examined predictors of alcohol misuse in the 30s and 40s.In person-centered trajectory analyses, a number of adolescent and young adult factors have been linked with increasing or consistently high trajectory groups, including alcohol and other substance use, truancy, and internalizing problems (Berg et al., 2019;Jackson & Sher, 2005;Meier et al., 2013;Virtanen et al., 2015;Warner et al., 2007).Results from prospective, variable-centered analyses lend additional support for each of these factors as predictors of early midlife alcohol outcomes (Merline et al., 2008;Schulenberg et al., 2016).Moreover, cross-sectional analyses have identified behavioral and psychosocial factors concurrently related to heavy drinking or alcohol problems in early midlife, such as other substance use and dependence, physical health, internalizing problems, employment and job demands, financial resources, and parenthood (Berg et al., 2013;Schulenberg et al., 2016).Nonetheless, correlates of developmental change in alcohol misuse through early midlife remain uncharacterized.

The current study
We studied two population-based samples in order to describe trajectories of alcohol misuse across adolescence through early midlife, estimate genetic and environmental contributions to individual differences in those trajectories, and identify factors associated with both initial levels of and changes in alcohol misuse.This study builds upon previous work in several ways.First, we applied a variable-centered approach to characterize trajectories of alcohol misuse from adolescence through early midlife.Second, we used data from a longitudinal study of Finnish twins to evaluate the degree to which individual differences in trajectories of alcohol misuse are explained by additive genetic, shared environmental, and unique environmental factors.A prior meta-analysis of twin and adoption studies suggests that approximately 50% of the variation in alcohol use disorder (AUD) is explained by genetic factors, and 10% is attributable to environmental factors that are shared by co-twins (e.g., family income, parental divorce, neighborhood characteristics) (Verhulst et al., 2015).Recent longitudinal studies have further demonstrated that changes in frequency and quantity of alcohol use across adolescence and adulthood are influenced by genetic factors (Drouard et al., 2023;Zellers et al., 2022).However, genetic and environmental components of alcohol misuse trajectories from adolescence through early midlife have not been studied.
Third, we investigated adolescent and young adult correlates of alcohol misuse trajectories.Consistent with the multilevel developmental contextual approach, we included correlates from a wide range of domains, such as other substance use, physical health, educational attainment, employment, and romantic relationships.We prioritized factors that have been associated with alcohol misuse in the 30s and 40s in previous work (Berg et al., 2013(Berg et al., , 2019;;Jackson & Sher, 2005;Meier et al., 2013;Merline et al., 2008;Schulenberg et al., 2016;Virtanen et al., 2015;Warner et al., 2007).Finally, we used population-based samples from both Finland (FinnTwin16) and the United States (the National Longitudinal Study of Adolescent to Adult Health) to investigate similarities and differences in developmental trajectories of alcohol misuse across cultural contexts.Because available measures of alcohol-related outcomes differed across samples, we focused on frequency of intoxication in FinnTwin16 and frequency of binge drinking in the National Longitudinal Study of Adolescent to Adult Health (Add Health).In what follows, we refer to both measures using the term "alcohol misuse" for parsimony.Our hypotheses and analytic plan were pre-registered using the Open Science Framework (doi:10.17605/OSF.IO/9D6 WB).

Participants
Participants were from two population-based longitudinal studies: FinnTwin16 and the National Longitudinal Study of Adolescent to Adult Health (Add Health).FinnTwin16 is a study of five consecutive birth cohorts of Finnish twins born 1975-1979(Kaidesoja et al., 2019)).Twins completed mailed surveys when they were 16 years old and were invited to participate in follow-up surveys at ages 17 (97% retention), 18 (97% retention), 25 (88% retention), and 35 (79% retention).Analyses were limited to 5,659 individuals (2,750 complete twin pairs, 159 singletons; 52% female; 32% monozygotic) who initiated alcohol use during the study period.For twin pairs who were discordant for lifetime history of alcohol use, only the co-twin who initiated alcohol use was retained in the analysis.Adolescent factors were from assessments at ages 16, 17, and 18, young adult factors were from the age 25 assessment, and the alcohol misuse outcome was measured at all five time points.Participants provided informed consent, and data were collected in accordance with Institutional Review Board and Research Ethics Committee guidelines.
Add Health is a nationally representative longitudinal study of more than 20,000 adolescents in the United States.Interviews were conducted in 1994 (Wave I), 1996 (Wave II; 76% retention), 2001-2002 (Wave III;80% retention), 2008-2009 (Wave IV;83% retention), and 2016-2018 (Wave V; 67% retention) (Harris et al., 2019).Participants ranged in age from 11 to 21 years at Wave I, 12-22 years at Wave II, 18-27 years at Wave III, 24-34 years at Wave IV, and 33-44 years at Wave V.The current sample was limited to individuals who initiated alcohol use during the study period (N = 18,026, 50% female).Participants self-identified as White (64%), Black (21%), Native American (4%), Asian (7%), or Other race/ethnicity (9%); 17% of participants also identified as Hispanic or Latino.Adolescent factors were from the Waves I and II assessments, young adult factors were from the Waves III and IV assessments, and the alcohol misuse outcome was measured at all five time points.To ensure that Add Health was comparable to FinnTwin16 regarding developmental timing, analyses of adolescent factors were limited to participants between ages 15 and 19 at the time of assessment.Analyses of young adult factors were limited to participants between ages 20 and 29.All assessments of alcohol misuse were used in the analyses, with no exclusions based on age.Participants provided written informed consent in accordance with Institutional Review Board guidelines.

Alcohol misuse
Alcohol misuse was assessed in FinnTwin16 using one item: "How often do you get really drunk?" Response options were "once a week or more," "about 1-2 times a month," "less often than that," and "never."Ordinal response options were recoded as a pseudocontinuous measure of days intoxicated per month using the midpoint of each response option, such that "once a week or more" was coded as 17, "about 1-2 times a month" was coded as 1.5, "less often than that" was coded as 0.5, and "never" was coded as 0. To disambiguate correlates of alcohol misuse from correlates of alcohol initiation, individuals who indicated that they never consume alcohol were recoded as missing.

Adolescent correlates
Correlates of alcohol misuse trajectories measured during adolescence included age of alcohol use onset, peer alcohol use, cigarette smoking, physical health, body mass index (BMI), sleeping difficulties, and grades.

Age of alcohol use onse
At age 16, participants reported their age when they first drank at least a glass of beer, at least a glass of wine, a long drink, and hard liquor.The earliest age reported across these four items was recorded as the participant's age of alcohol use onset.

Peer alcohol use
At ages 16, 17, and 18, participants were asked, "What proportion of your same-sex peers drink [beer, wine, liquor] every now and then?"Response options were 1 = "almost everyone," 2 = "most," 3 = "half," 4 = "some," and 5 = "hardly any."Items were reversecoded, and peer alcohol use was recorded as the participant's maximum value across items and assessments.

Smoking
Cigarette smoking was measured at ages 16, 17, and 18 using one item: "Which of the following best describes your present smoking habits?"At age 16, response options were 1 = "I have never smoked," 2 = "I smoke less often than once a week," 3 = "I smoke once or more a week but not every day," and 4 = "I smoke once or more daily."At ages 17 and 18, the maximal response options were "I smoke every day but no more than 9 cigarettes per day" and "I smoke at least 10 cigarettes a day;" these responses options were combined to remain consistent with the age 16 assessment.Adolescent smoking was then recorded as the participant's maximal value across assessments.

Overall physical health
At ages 16, 17, and 18, participants were asked whether they viewed their health as 1 = "very good," 2 = "rather good," 3 = "mediocre," 4 = "rather poor," or 5 = "very poor."This item was reverse-coded, and the participant's minimum value across assessments was utilized in the analysis.

BMI
At ages 16, 17, and 18, participants were asked to report their current height and weight.BMI was calculated as weight (in kilograms) divided by height-squared (in meters-squared).The participant's maximum value across assessments was used in the analysis.

Sleeping difficulties
Sleeping difficulties at ages 16, 17, and 18 were assessed using one item: "During the past six months, how often have you had difficulty getting to sleep or staying asleep?"Response options included 1 = "seldom or never," 2 = "about once a month," 3 = "about once a week," and 4 = "almost every day."Adolescent sleeping difficulties was then recorded as the participant's maximum value across assessments.

Grades
Participants reported on their grades at age 16 using one item: "What kind of grades did you receive last term?Compared to the average in your class or course, were they much better [1], somewhat better [2], about average [3], somewhat below average [4], or considerably below average [5]?"This item was reversecoded, such that 1 = "considerably below average" and 5 = "much better" than the average.

Young adult correlates
Correlates measured in young adulthood included cigarette smoking, nicotine dependence, other drug use, educational attainment, employment, job satisfaction, financial status, cohabitation status, number of romantic partnerships involving cohabitation, relationship satisfaction, parenthood, psychological distress, physical activity, importance of religion, frequency of religious service attendance, satisfaction with free time, physical health, BMI, and sleeping difficulties.All young adult correlates were measured at the age 25 assessment.

Smoking
Smoking behavior was measured using one item: "Which of the following best describes your present smoking habits?"Response options were 1 = "I smoke at least 20 cigarettes per day," 2 = "I smoke 10-19 cigarettes per day," 3 = "I smoke at most 9 cigarettes a day," 4 = "I smoke once a week or more often but not daily," 5 = "I smoke less than once a week," 6 = "I have quit smoking," and 7 = "I have never smoked."This item was reverse-coded.

Nicotine dependence
Participants who reported smoking at least 20 cigarettes per day were coded as meeting criteria for nicotine dependence (1), and all others were coded as 0.

Educational attainment
Participants reported their highest level of education as primary school (1), high school (2), trade school (3), vocational school (4), or university or vocational college of university standing (5).

Employment
Participants reported whether they were primarily working outside the home, working at home, a student, unemployed, in the military, or doing something else.In addition, participants recorded how many hours per week they work for wages.These measures were recoded as follows: unemployed, not in the workforce (working at home, student, or doing something else), employed part-time (working outside the home less than 39 hours per week), and employed full-time (working outside the home 40þ hours per week or in the military).A set of dummy-coded variables was created, with employed full-time as the reference category.

Job satisfaction
Job satisfaction was measured using one item: "Are you satisfied with your success at work and in your studies?"Response options were 1 = "completely," 2 = "mostly," 3 = "somewhat," 4 = "mostly not," and 5 = "not at all."This item was reverse-coded.

Cohabitation
Participants were asked whether they lived with a spouse or romantic partner, their parents, alone, alone with children, or someone else.Participants who reported that they lived with a spouse or romantic partner were coded as 1.All others were coded as 0.

Number of romantic partnerships with cohabitation
Participants reported how many romantic relationships they had where they lived together with someone.Response options were 1 = "none," 2 = "one," 3 = "two," and 4 = "three or more."

Relationship satisfaction
Participants reported on the degree to which they were satisfied with their relationship with their partner using the following response options: 1 = "not at all," 2 = "mostly not," 3 = "somewhat," 4 = "mostly," and 5 = "completely."

Psychological distress
Psychological distress was measured using the 20-item General Health Questionnaire (Goldberg, 1972) (α = 0.92).Each item was rated on a 4-point Likert scale, with higher values indicating greater psychological distress.Among individuals with complete data on 10 or more items, psychological distress was recorded as a prorated mean score.

Work-related physical activity
Participants indicated whether their work was 1 = "largely sedentary work, not much walking during the working day," 2 = "sedentary or standing work involving some walking, but not much lifting or carrying," 3 = "working involving a lot of walking, lifting, or carrying," or 4 = "heavy manual work, involving lifting or carrying heavy objects, digging, logging, etc." Physical activity during leisure time Physical activity was measured using two items: "How often do you exercise in your leisure time?" and "How long do you exercise per occasion?"The product of these two items was computed as a measure of time spent exercising per month.

Frequency of religious service attendance
Religious service attendance was evaluated using one item: "Not counting weddings, funerals, and baptisms, how often do you go to church or other religious events?"(1 = "once a week," 2 = "once a month," 3 = "once a year," 4 = "less often," 5 = "not at all").This item was reverse-coded.

Satisfaction with free time
Participants reported on the degree to which they were satisfied with their leisure time at home and outside the home (1 = "completely," 2 = "mostly," 3 = "somewhat," 4 = "mostly not," and 5 = "not at all").Each of these items was reverse-coded.

Sleeping difficulties
Sleeping difficulties were measured using one item: "During the past six months, how often have you had difficulty getting to sleep or staying asleep?"Response options included 1 = "seldom or never," 2 = "about once a month," 3 = "about once a week," and 4 = "almost every day."

Covariates
Participant sex (0 = female, 1 = male) was included as a covariate in all analyses.

Alcohol misuse
One item was used as an index of alcohol misuse: "Over the past 12 months, on how many days did you drink 5 or more drinks in a row?" Response options included "none," "1 or 2 days," "once a month or less," "2 or 3 days a month," "1 or 2 days a week," "3-5 days a week," and "every day or almost every day."Ordinal response options were recoded as a pseudo-continuous measure of binge drinking days per month using the mid-point of each response option.Individuals who indicated that they had not consumed alcohol in the past year were coded as missing.

Adolescent correlates
Correlates of alcohol misuse trajectories measured during adolescence included age of alcohol use onset, peer alcohol use, cigarette smoking, physical health, BMI, and grades.

Age of alcohol use onset
At Waves I, II, and IV, participants reported their age when they first had a drink of beer, wine, or liquor while not with their parents.

Peer alcohol use
At Waves I and II, peer alcohol use was measured using one item: "Of your three best friends, how many drink alcohol at least once a month?"Responses ranged from 0 to 3. Peer alcohol use was recorded as the participant's maximum value across assessments.

Smoking
Cigarette smoking was measured at Waves I and II using two items: "During the past 30 days, on how many days did you smoke cigarettes?" and "During the past 30 days, on the days you smoked, how many cigarettes did you smoke each day?"The product of these two items was computed to create a measure of cigarettes smoked per month, and adolescent smoking was recorded as the participant's maximum value across assessments.

Overall physical health
At Waves I and II, participants rated their health as 1 = "excellent," 2 = "very good," 3 = "good," 4 = "fair," or 5 = "poor."The item was reverse-coded, and the participant's minimum value across assessments was used in the analysis.

BMI
Participants reported their current height and weight at Waves I and II.BMI was calculated as weight (in kilograms) divided by height-squared (in meters-squared).The participant's maximum value across assessments was utilized in the analysis.

Grades
At Waves I and II, participants reported their grade in English/ Language Arts, Mathematics, History/Social Studies, and Science (1 = A, 2 = B, 3 = C, 4 = D or lower).Each item was reverse-coded, and the mean was computed across subjects.The participant's grades were then recorded as the minimum value across assessments.

Young adult correlates
Correlates measured in young adulthood included cigarette smoking, nicotine dependence, cannabis problems, educational attainment, employment, job satisfaction, financial status, financial difficulties, number of romantic partnerships involving cohabitation, relationship satisfaction, parenthood, psychological distress, physical activity, importance of religion, frequency of religious service attendance, frequency of other religious activities, physical health, and BMI.

Smoking
Cigarette smoking was measured at Waves III and IV using two items: "During the past 30 days, on how many days did you smoke cigarettes?" and "During the past 30 days, on the days you smoked, how many cigarettes did you smoke each day?"The product of these two items was used as a measure of cigarettes smoked per month.Young adult smoking was then recorded as the participant's maximum value across assessments.

Nicotine dependence
Nicotine dependence was assessed at Waves III and IV using the Fagerstrom Test for Nicotine Dependence (FTND) (Heatherton et al., 1991) (α = 0.77 at Wave III, α = 0.79 at Wave IV).The participant's maximum FTND score was used in the analysis.

Cannabis problems
Number of DSM-IV cannabis use disorder symptoms was evaluated at Wave IV.

Educational attainment
At Wave IV, participants reported on their highest level of education: 8 th grade or less, some high school, high school graduate, some vocation/technical training, completed vocational/ technical training, some college, completed college, some graduate school, completed a master's degree, some graduate training beyond a master's degree, completed a doctoral degree, some postbaccalaureate professional education, or completed post-baccalaureate professional education.

Employment
Employment status at Waves III and IV was evaluated using one item: "Are you currently working for pay for at least 10 hours a week?"Participants also reported on how many hours per week they spend working for pay.These measures were recoded as follows: not in the workforce (did not endorse working for pay at least 10 hours per week), employed part-time (working 10-39 hours per week), and employed full-time (working 40þ hours per week).Dummy-coded variables were created with employed full-time as the reference category.Participants who differed in their employment status across waves were categorized as "not in the workforce" if they met these criteria at either assessment and as "employed part-time" if they were employed part-time at one assessment and employed full-time at another assessment.

Job satisfaction
At Waves III and IV, job satisfaction was measured using one item: "How satisfied are you with this job, as a whole?"Response options were 1 = "extremely satisfied," 2 = "satisfied," 3 = "neither satisfied nor dissatisfied," 4 = "dissatisfied," and 5 = "extremely dissatisfied."This item was reverse-coded, and the participant's minimum satisfaction with their job was used in the analysis.

Financial status
Financial status was measured at Wave IV using one item: "Suppose you were to sell all of your possessions and pay off your debts.Would you have something left over [1], break even [2], or be in debt [3]?"

Financial difficulties
At Waves III and IV, participants were asked whether they have been without telephone service, were unable to pay the full amount of the rent or mortgage, were evicted for not paying the rent or mortgage, did not pay the full amount of a utility bill, or had the service turned off by the gas or electric company.The participant's maximum number of financial problems across Waves III and IV was used in the analysis.

Number of romantic partnerships with cohabitation
At Waves III and IV, participants were asked, "With how many people have you lived in a marriage-like relationship?"The participant's maximum number of cohabiting partners was incorporated in the analysis.

Relationship satisfaction
Relationship satisfaction was measured at Wave III using one item: "In general, how satisfied are you with your relationship with your partner"?Response options were 1 = "very satisfied," 2 = "somewhat satisfied," 3 = "neither dissatisfied or satisfied," 4 = "somewhat dissatisfied," and 5 = "very dissatisfied."This item was reverse-coded, such that higher values reflect greater relationship satisfaction.

Parenthood
Participants who reported at least one live birth by Waves III or IV were coded as 1, and all others were coded as 0.

Psychological distress
At Waves III and IV, psychological distress was assessed using 9 items from the Center for Epidemiologic Studies Depression Scale (Andresen et al., 1994) (α = 0.81 at Wave III, α = 0.81 at Wave IV).Each item was rated on a 4-point scale, with higher values indicating greater psychological distress.A sum score was computed, and the participant's maximum value across assessments was used in the analysis.

Work-related physical activity
At Wave III, work-related physical activity was evaluated using four items, which asked how many hours per week the participant spent doing hard, moderate, and light physical work, and being seated.Based on which level of physical activity characterized their work the majority of the time, work-related physical activity was coded as 1 = seated, 2 = light physical work, 3 = moderate physical work, and 4 = hard physical work.

Physical activity during leisure time
Physical activity was measured at Waves III and IV using 7 items, which asked how many times the participant had participated in a series of activities (e.g., team sports, cycling, jogging) within the past week.For each assessment, a total sum score was calculated to represent the total number of times that the participant exercised in the past week; the minimum value across Waves III and IV was used in the analysis.

Importance of religion
Participants were asked, "How important is religion to you?" (1 = "not important," 2 = "somewhat important," 3 = "very important," and 4 = "more important than anything else").The minimum value across Waves III and IV was utilized in the analysis.

Frequency of religious service attendance
Participants reported on their religious service attendance using one item: "In the past 12 months, how often did you attend religious services?"(0 = "never," 1 = "a few times," 2 = "once a month," 3 = "2 or 3 times a month," 4 = "once a week," and 5 = "more than once a week").The minimum value across Waves III and IV was used in the analysis.

Frequency of other religious activities
Participants reported on their attendance at other religious activities using one item: "In the past 12 months, how often did you attend church activities outside of regular worship service?" (0 = "never," 1 = "a few times," 2 = "once a month," 3 = "2 or 3 times a month," 4 = "once a week," and 5 = "more than once a week").The minimum value across Waves III and IV was utilized in the analysis.

Overall physical health
At Waves III and IV, participants rated their health as 1 = "excellent," 2 = "very good," 3 = "good," 4 = "fair," and 5 = "poor."This item was reverse-coded, and physical health was recorded as the participant's minimum value across assessments.

BMI
Participants reported their current height and weight at Waves III and IV.BMI was calculated as weight (in kilograms) divided by height-squared (in meters-squared), and young adult BMI was recorded as the participant's maximum value across assessments.

Covariates
Participant sex (0 = female, 1 = male), racial/ethnic identity, and year of assessment were included as covariates.Race/ethnicity was aggregated into the following classifications: Non-Hispanic White, Hispanic White, non-Hispanic Black, Hispanic Black, and Other.A set of dummy-coded variables was created, with non-Hispanic White serving as the reference group.

Statistical analysis
We characterized trajectories of alcohol misuse from adolescence through early midlife using a mixed-effects growth curve model based on orthogonal variance components (McArdle, 2006).A mixed-effects growth curve model based on orthogonal variance components is written as is the outcome of interest (in this case, alcohol misuse), and ∝ represents the timing of assessments.According to the above equation, for each individual (n = 1 to N), their alcohol misuse is described in terms of a unique score for alcohol misuse at baseline (U0); a unique score for linear change in alcohol misuse across the study period (U1); a score describing the covariance between their initial level of alcohol misuse and their change in alcohol misuse over time (C01), which is uncorrelated with the unique scores (U0, U1); and measurement error at each assessment (U[t]).In the full sample, initial levels of alcohol misuse and changes in alcohol misuse over time are thus described by fixed means (β 0 , β 1 ), as well as random variances (σ u0 2 , σ u1 2 ) and a covariance (σ c01 2 ) describing inter-individual variation in trajectories of alcohol misuse.The model can be further expanded to include unique and common scores for non-linear change in alcohol misuse over time.
To ensure that analyses of FinnTwin16 and Add Health were comparable, age 16 was used as the "baseline" in both samples (i.e., time was fixed to 0 at age 16).Analyses accounted for nesting of data within individuals (Add Health and FinnTwin16) and within families (FinnTwin16 only).We constructed interceptonly, linear, and quadratic growth models, then selected the bestfitting model based on the Akaike information criterion (AIC), the Bayesian information criterion (BIC), and likelihood ratio tests.
Next, we leveraged the twin design of FinnTwin16 to examine genetic and environmental contributions to trajectories of alcohol misuse.We used the longitudinal biometric variance component model (McArdle, 2006), which expands upon the mixed-effects growth curve model by describing contributions of additive genetic (A), shared environmental (S), and unique environmental (E) factors to initial levels of alcohol misuse and changes in alcohol misuse over time.The longitudinal biometric variance component model invokes the same assumptions as the classical twin design: namely, that monozygotic twins share 100% of their genes, dizygotic twins share 50% of their genes, and the degree to which twins share family and community experiences is equal for monozygotic and dizygotic twins.The unique environment includes experiences not shared by twins and random error.
Finally, to identify adolescent and young adult factors associated with trajectories of alcohol misuse, we conducted two sets of analyses: one with adolescent correlates included as predictors, and one with young adult factors included as predictors.Significant predictors (p < .05)were carried forward into a combined model to evaluate whether associations with trajectories of alcohol misuse remained significant after accounting for factors from other developmental periods.Models included the main effect of each factor, which reflects the association between the adolescent or young adult factor and initial levels of alcohol misuse, as well as an interaction term between the factor and time, which reflects the degree to which the adolescent or young adult factor was associated with change in alcohol misuse over time.
Sex was included as a covariate in analyses of both samples.Racial/ ethnic identity and year of assessment were included as covariates in analyses of Add Health only because FinnTwin16 is ethnically homogeneous.All analyses were conducted using SAS PROC MIXED in SAS 9.4 (©2002-2012 SAS Institute Inc., Cary NC US).

Descriptive statistics
Table 1 provides descriptive statistics for the study variables.Mean levels of alcohol misuse are presented by wave of assessment for ease of presentation, though age was used as the metric for time in the primary analyses.The distribution of alcohol misuse is shown separately by sex in Figures S1 and S2 for FinnTwin16 and Add Health, respectively.
In FinnTwin16, the mean value for frequency of alcohol misuse was lowest at the age 16 assessment and highest at the age 25 assessment.On average, participants reported drinking to intoxication once every 1 to 2 months (i.e., 0.77 days per month) at age 16.At age 25, participants drank to intoxication an average of 3 days per month.In Add Health, the mean value for frequency of alcohol misuse among past-year drinkers was lowest at the Wave V assessment (mean age = 37.56 years) and highest at the Wave II assessment (mean age = 16.24years), such that, on average, participants reported binge drinking 1.84 days per month at Wave V and 2.49 days per month at Wave II.

Trajectories of alcohol misuse
We constructed intercept-only, linear, and quadratic growth curve models to represent changes in alcohol misuse from adolescence through early midlife.As shown in Table 2, a quadratic growth curve model provided the best fit to the data in both FinnTwin16 and Add Health: The quadratic model was associated with lower AIC and BIC values than the intercept-only and linear models, and likelihood ratio tests indicated that the linear model provided significantly worse model fit when compared to the quadratic model1 .
The quadratic growth curve model includes fixed effects, which represent the expected mean at age 16 (β 0 ), the expected linear change in alcohol misuse at age 16 (β 1 ), and how the rate of change in alcohol misuse varies per decade (β 2 ).The model also includes random effects, which represent individual differences in alcohol misuse trajectories.Across FinnTwin16 and Add Health, there was significant inter-individual variability in initial levels of alcohol misuse at age 16 (σ u0 2 ) and in linear (σ u1 2 ) and non-linear (σ u2 2 ) change in alcohol misuse over time.The intercept and linear slope showed a negative covariance (σ c01 2 ), meaning that individuals who reported higher initial levels of alcohol misuse had a slower increase in alcohol misuse.Similarly, values for the linear and quadratic slopes were negatively related to one another (σ c12 2 ), whereas the intercept and quadratic slope were positively related (σ c02 2 ).In Figure 1, the expected frequency of alcohol misuse over time (derived from the quadratic model parameters) and expected deviation from the average trajectory of alcohol misuse are plotted separately by sex.For both males and females in FinnTwin16 (Fig. 1a) and males in Add Health (Fig. 1d), frequency of alcohol misuse increased after age 16, peaked in early adulthood, and declined thereafter.For females in Add Health, frequency of alcohol misuse declined from age 16 through age 35.In FinnTwin16, the predicted age of maximal alcohol misuse was 27 years for females and 28 years for males.Inter-individual variability in alcohol misuse also increased from age 16 into young adulthood, peaking at age 27 in both male and female participants and declining thereafter (Fig. 1b).In Add Health, maximal alcohol misuse occurred at an earlier age: 16 years for females and 22 years for males.Variability in alcohol misuse declined from age 16,

Development and Psychopathology
reached a minimum at ages 22 and 23 in males and females, respectively, then increased across the remainder of the study period (Fig. 1e).

Genetic and environmental components of alcohol misuse trajectories
In FinnTwin16, we applied the longitudinal biometric variance component model to describe contributions of additive genetic, shared environmental, and unique environmental factors to interindividual variability in alcohol misuse trajectories.Model results are presented in Table 3.When combined across random effect parameters using the method described by McArdle (2006), additive genetic factors accounted for 0.3% of the total interindividual variability in alcohol misuse trajectories from adolescence through early midlife.Shared environmental factors accounted for 38.0%, unique environmental factors accounted for 58.3%, and time-specific error accounted for 3.3% of the variance.Though the contributions of additive genetic factors were small in magnitude, only the additive genetic component of the covariance between the intercept and quadratic slope (σ 2 ac02 ) could be fixed to zero without a significant decrease in model fit (Table 3).
To ease interpretation, we plotted model-implied additive genetic, shared environmental, and unique environmental contributions to deviations from the expected trajectory of alcohol misuse as a function of time (McArdle, 2006).As shown in Figure 1c, variability in alcohol misuse attributable to shared environmental factors increased from age 16 through age 28 and declined thereafter.Variation attributable to additive genetic factors remained small in magnitude across the study period.

Adolescent and young adult correlates of alcohol misuse trajectories
Next, we evaluated associations of a series of adolescent and young adult factors with trajectories of alcohol misuse.In FinnTwin16 and Add Health, we constructed two initial models: The first included adolescent correlates of alcohol misuse trajectories, and the second included young adult correlates.For each adolescent and young adult factor, we estimated its relationship with frequency of alcohol misuse at age 16 and the degree to which the variable moderated linear and quadratic change in alcohol misuse over time.If a variable was statistically significantly associated with the intercept, linear slope, or quadratic slope, it was carried forward into a combined model.Results from the adolescent, young adult, and combined models are summarized in Figure 2.

Correlates of alcohol misuse trajectories in FinnTwin16
In FinnTwin16, the combined model included seven variables measured in adolescence age of alcohol use onset, peer alcohol use, smoking, physical health, BMI, sleeping difficulties, and grades and seven variables measured in young adulthood smoking, other drug use, educational attainment, current cohabitation, number of partnerships involving cohabitation, parenthood, and physical health.For brevity, we focus our review below on statistically significant associations.

Associations with initial levels of alcohol misuse
In the combined model, adolescents who reported a higher proportion of drinking peers, greater cigarette smoking, greater BMI, and more sleeping difficulties indicated more frequent alcohol misuse at age 16.On the other hand, adolescents with better physical health and higher grades exhibited less frequent alcohol misuse.Lower levels of smoking, more frequent use of other drugs, lower educational attainment, more romantic partnerships involving cohabitation, and better physical health at age 25 were also associated with higher initial levels of alcohol misuse.

Associations with change in alcohol misuse over time
The interactions of adolescent and young adult smoking, adolescent BMI, other drug use, cohabitation, parenthood, and young adult physical health with time were statistically significant, suggesting that these factors were associated with linear change in alcohol misuse.In addition, peer alcohol use, adolescent BMI, young adult smoking, other drug use, cohabitation, parenthood, and young adult physical health were related to quadratic change in alcohol misuse.The model-implied trajectories of alcohol misuse across levels of these variables are presented in Figure 3a.

Correlates of alcohol misuse trajectories in Add Health
In Add Health, the combined model included four variables measured in adolescence age of alcohol use onset, peer alcohol use, smoking, and grades and seven variables measured in young adulthood smoking, cannabis problems, educational attainment, job satisfaction, number of romantic partnerships involving cohabitation, parenthood, and psychological distress.

Associations with initial levels of alcohol misuse
In the combined model, adolescents who reported an earlier age of alcohol use onset, affiliated with a greater number of drinking peers, and endorsed greater cigarette smoking reported more frequent alcohol misuse at age 16.Lower educational attainment, greater job satisfaction, and higher young adult psychological distress were also related to greater alcohol misuse at age 16.

Associations with change in alcohol misuse over time
The interactions of adolescent and young adult smoking, cannabis problems, job satisfaction, number of romantic partnerships involving cohabitation, psychological distress, and parenthood with time were statistically significant, suggesting that these factors were related to linear change in alcohol misuse.Further, adolescent smoking, cannabis problems, psychological distress, and parenthood were related to quadratic change in alcohol misuse.For each variable associated with linear or non-linear change in alcohol misuse, the model-implied trajectories are presented in Figure 3b.

Discussion
Our aims were to (1) characterize trajectories of alcohol misuse from adolescence through early midlife, (2) describe genetic and environmental contributions to individual differences in those trajectories, (3) identify adolescent and young adult factors associated with initial levels of alcohol misuse and changes in alcohol misuse over time, and (4) compare and contrast findings across two population-based cohorts.Consistent with prior work on rates of alcohol misuse across the lifespan (Jackson & Sartor, 2016;Lee & Sher, 2018), the expected trajectory of alcohol misuse from adolescence through early midlife generally followed an inverted U-shaped curve: Frequency of alcohol misuse increased after age 16, peaked in early adulthood, and declined thereafter.Nonetheless, we also observed substantial inter-individual variability in trajectories of alcohol misuse, which was primarily attributable to environmental factors.
Overall, the developmental course of alcohol misuse was similar across FinnTwin16, a population-based sample of Finnish twins,

Development and Psychopathology
and Add Health, a population-based sample from the United States, with two exceptions.First, while the expected trajectory of alcohol misuse followed an inverted U-shaped curve across sex in FinnTwin16, frequency of alcohol misuse declined across the study period in female individuals in Add Health.This pattern of results may be driven by our decision to code past-year nondrinkers as missing.As a result, only 16-year-olds who report past-year alcohol use (a relatively high-risk group) contributed to the predicted value for frequency of alcohol misuse at age 16, potentially increasing the estimated intercept.To evaluate this possibility, we repeated the analyses with past-year nondrinkers coded as zero.In these supplementary analyses, the predicted frequency of alcohol misuse at age 16 was lower, and the expected inverted U-shaped curve was observed across sex and samples (Figure S3).Furthermore, and as expected, when past-year nondrinkers were coded as zero, the predicted age at peak alcohol misuse was 27 years for females and 28 years for males in FinnTwin16, and 20 years for females and 23 years for males in Add Health.
This highlights another notable difference in the pattern of results across samples: The age at peak alcohol misuse was substantially earlier in Add Health when compared to FinnTwin16.One potential explanation is that escalation of binge drinking during the college years, which is reliably observed in the United States (Schulenberg & Patrick, 2011), may contribute to an earlier age of maximal alcohol misuse: Indeed, prior studies of drinking patterns in the United States have similarly noted that the prevalence and frequency of binge drinking peaks in the early 20s (Patrick et al., 2016(Patrick et al., , 2019)).Another non-mutually exclusive explanation is that role transitions associated with reductions in alcohol misuse (e.g., marriage, parenthood) generally occur later in Finland than in the United States (National Center for Health Statistics, 2016;Statistics Finland, 2020).
Next, in FinnTwin16 only, we estimated the contributions of genetic and environmental factors to individual differences in alcohol misuse trajectories.It was surprising that additive genetic factors accounted for a very small (but statistically significant) proportion of the variance, particularly in view of substantial evidence that AUD is moderately heritable (Verhulst et al., 2015).Prior cross-sectional analyses in FinnTwin16 have also supported genetic influences on alcohol misuse (Dick et al., 2011;Penninkilampi-Kerola et al., 2005;Viken et al., 1999).Of note, additive genetic factors accounted for little variance in the unique component of the intercept in the present analyses.However, this parameter is not directly comparable to parameter estimates from cross-sectional univariate twin models because the biometric growth curve model accounts for the correlations among growth factors, partitioning the variance into components that are unique to a specific growth factor and shared between growth factors.Additive genetic, shared environmental, and unique environmental contributions to each of those variance components are then estimated.
Therefore, to replicate the cross-sectional findings from previous work, we conducted cross-sectional univariate twin models to investigate the etiology of alcohol misuse at each assessment.We fitted the full univariate ACE model, then tested simpler models without shared environmental influences (AE model) and without additive genetic influences (CE model).At age 16, the full ACE model provided the best fit to the data (Table S1).Approximately 47%, 28%, and 25% of the variance in age 16 alcohol misuse was attributable to additive genetic, shared environmental, and unique environmental factors, respectively (Table S2).For the remaining assessments, shared environmental influences on alcohol misuse could be removed without a significant change in model fit (Table S1), and the estimated heritability of alcohol misuse ranged from 59% to 77% (Table S2).
Thus, although our finding that genetic factors account for little variance in trajectories of alcohol misuse was surprising, it echoes a broader point from the developmental literature, which is that cross-sectional snapshots do not necessarily capture longitudinal change (Di Biase et al., 2023).For instance, a number of studies have shown minimal contributions of the shared environment to alcohol use and problems in adulthood (Dick, 2011;van Beek et al., 2012), and we replicated this finding in our own cross-sectional analyses (see above).Conversely, our analyses of longitudinal change in alcohol misuse through early midlife suggest that shared environmental influences contribute considerably to the overall shape of alcohol misuse trajectories: Perhaps features of the shared environment that are relevant to adolescent drinking behavior, such as alcohol availability in the home (Komro et al., 2007), parental divorce (Salvatore et al., 2023), or local community-level factors (Kristjansson et al., 2020), have lasting effects on an individual's overall course of alcohol misuse but are less relevant to cross-sectional variation in alcohol misuse among adults.Nonetheless, these findings warrant replication in other samples.
Finally, we investigated whether a series of adolescent and young adult factors were related to alcohol misuse frequency at age 16 and to changes in alcohol misuse over time.We focus on three findings that were observed across both samples.First, substancerelated variables were associated with alcohol misuse trajectories in both FinnTwin16 and Add Health.Adolescent cigarette smoking was related to alcohol misuse at age 16 and to changes in alcohol misuse over time.Moreover, higher levels of smoking and other drug use in young adulthood were associated with a steeper increase in alcohol misuse and higher predicted frequency of alcohol misuse across the study period.For example, among individuals who used illicit drugs 20 or more times in FinnTwin16, the predicted frequency of alcohol misuse at age 35 was 4 days intoxicated per month (compared to 1 day per month among individuals who never used other drugs).These findings are consistent with prior longitudinal studies (Chassin et al., 2002;Merline et al., 2008;Schulenberg et al., 2016) and underscore the potential long-term impact of efforts to reduce substance use among adolescents and young adults (Griffin & Botvin, 2010;White & Rabiner, 2012).
Second, individuals who were parents by young adulthood exhibited less frequent alcohol misuse than those who remained childless.The negative association between parenthood and alcohol misuse is in line with recent work (Rose et al., 2022) and with role compatibility theory, which suggests that individuals with high levels of substance use may delay marriage or parenthood (role selection) and that adopting a social role incompatible with substance-related behaviors leads to reduced substance use (role socialization) (Yamaguchi & Kandel, 1985).Third, we did not find evidence that employment and financial status in young adulthood were related to trajectories of alcohol misuse, which contrasts with prior cross-sectional studies of heavy drinking and AUD in early midlife (Berg et al., 2013;Schulenberg et al., 2016).Though job loss and financial difficulties may cooccur with alcohol misuse and problems, they did not modify the developmental course of alcohol misuse in the present study.

Limitations
These findings should be considered in view of several limitations.First, alcohol misuse was assessed using only one item, and samplebased differences in the measurement of alcohol misuse (frequency of intoxication in FinnTwin16, frequency of binge drinking in Add Health) may contribute to differences in the pattern of results.Second, drinking to intoxication is not included in other definitions of alcohol misuse (National Institute on Alcohol Abuse and Alcoholism, n.d.), which focus on binge and heavy drinking.However, a study of perceived drunkenness among Finnish adolescents found that girls and boys who reported moderate drunkenness had consumed, on average, 5 and 6 drinks, respectively (Lintonen et al., 2004), suggesting that drinking to intoxication may be a reasonable indicator of alcohol misuse.Third, due to data availability, our analyses only considered frequency of intoxication (FinnTwin16) and frequency of binge drinking (Add Health) as indicators of alcohol misuse.Therefore, it will be important for future research to consider other dimensions of alcohol misuse (e.g., heavy drinking, high-intensity drinking), as well as measures of clinically significant alcohol problems.The pattern and correlates of alcohol trajectories are likely to differ based on the alcohol-related outcome under study: Prior work suggests that indices of alcohol consumption, misuse, and problems have an overlapping, but partially distinct, genetic architecture (Dick et al., 2011;Kranzler et al., 2019), and different predictors emerge in analyses of heavy drinking versus AUD (Merline et al., 2008).As a result, the findings observed in the present study may or may not generalize to other alcohol-related outcomes.
Fourth, our findings do not provide evidence for causality.For example, when considering the negative association between parenthood and change in alcohol misuse, we cannot distinguish whether becoming a parent is associated with reductions in alcohol misuse or individuals with more frequent alcohol misuse are less likely to become parents.Additional work is needed to differentiate causes and consequences of alcohol misuse.Fifth, analyses focused on specific patterns of alcohol consumption but did not control for participants' overall alcohol intake.

Conclusions
On average, frequency of alcohol misuse increases across adolescence, peaks in young adulthood, and declines thereafter.Even so, individuals show varying trajectories of alcohol misuse across development.As hypothesized by the multilevel developmental contextual framework, heterogeneity in the course of alcohol misuse is related to person-level, proximal environmental, and distal environmental factors.For example, one's own substance use, physical health, and mental health (person-level factors), as well as peer substance use, cohabitation, and parenthood (proximal environments), moderated trajectories of alcohol misuse in the present study.Efforts to reduce alcohol misuse and problems in early midlife may consider these factors as the basis for selective intervention or as modifiable intervention targets.Distal environmental factors also played a role: The average age at peak alcohol misuse varied across samples from Finland and the United States, highlighting the importance of considering the broader cultural context when determining the optimal timing of prevention efforts.Ultimately, our findings underscore that alcohol misuse is a highly complex, developmentally dynamic process.Efforts to describe and reduce alcohol misuse in early midlife must consider this inherent complexity.
Supplementary material.The supplementary material for this article can be found at https://doi.org/10.1017/S0954579424000543.

Figure 1 .
Figure 1.Trajectories of alcohol misuse from adolescence through early midlife.(a) Expected mean frequency of alcohol misuse based on the quadratic growth curve model parameters in FinnTwin16.(b) Expected deviation from the mean trajectory of alcohol misuse in FinnTwin16.(c) Expected contributions of additive genetic factors, a[t]; shared environmental factors, s[t]; and unique environmental factors, e[t], to deviation from the expected trajectory of alcohol misuse, presented as a function of age.(d) Expected mean frequency of alcohol misuse based on the quadratic growth curve model parameters in Add Health.(e) Expected deviation from the mean trajectory of alcohol misuse in Add Health.
Random variances with the subscript u0 represent variability in U0, a unique score for alcohol misuse at baseline.The subscript u1 refers to variability in U1, a unique score for linear change in alcohol misuse, and the subscript u2 refers to variability in U2, a unique score for quadratic change in alcohol misuse.The subscript c01 refers to the covariance between initial levels and linear change in alcohol misuse over time; c02 and c12 refer to the covariances between the intercept and quadratic slope, and between the linear and quadratic slopes, respectively.Contributions of additive genetic, shared environmental, and unique environmental factors to each of these random variance parameters are denoted by the subscripts a, s, and e, respectively.σ 2 u represents time−specific measurement error.Est = estimate; CI = confidence interval; AIC = Akaike Information Criterion; BIC = Bayesian Information Criterion.

Figure 2 .
Figure 2. Adolescent and young adult correlates of alcohol misuse trajectories.Adolescent and young adult factors were first examined in separate models.For each factor, we estimated its relationship with frequency of alcohol misuse at age 16 (main effect) and the degree to which the variable modified linear (predictor × time) and quadratic (predictor × time-squared) change in alcohol misuse.Results from these period-specific models are shown in green for FinnTwin16 and in purple for Hdd Health.If a variable was statistically significantly (p < .05)associated with the intercept, linear slope, or quadratic slope, it was carried forward into a combined model (shown in orange for FinnTwin16 and pink for Add Health).Statistically significant (p < .05)parameter estimates are shown as a filled circle, and non-significant estimates are shown as an open circle.Across models, ordinal and continuous predictors were standardized, but the outcome was not.Therefore, the beta estimate represents the expected change in days intoxicated per month (FinnTwin16) or binge drinking days per month (Add Health) associated with a 1-standard deviation increase in the predictor.Error bars represent 95% confidence intervals.Ad = adolescent; YA = young adult; # = number; freq = frequency; sat = satisfaction.

Figure 3 .
Figure 3. Predicted frequency of alcohol misuse as a function of adolescent and young adult predictors.(a) peer alcohol use, adolescent cigarette smoking, adolescent body mass index, young adult cigarette smoking, other drug use, cohabitation, parenthood, and young adult physical health were associated with linear and/or non-linear change in alcohol misuse in FinnTwin16.Trajectories of alcohol misuse are presented across levels of each of these predictors.(b) adolescent and young adult cigarette smoking, cannabis dependence symptoms, job satisfaction, number of romantic partnerships involving cohabitation, psychological distress, and parenthood were associated with linear and/or non-linear change in alcohol misuse in Add Health.Trajectories of alcohol misuse are presented across levels of each of these predictors.Pred = predicted; prop = proportion; < 1x a wk = less than one time per week; BMI = body mass index; > 1x a wk = more than one time per week; SD = standard deviation; sx = symptoms.

Table 1 .
Descriptive statistics for the primary study variables Note.Descriptive statistics are not always comparable across samples, as FinnTwin16 and Add Health often used different measures of the same construct.See the Method section for additional details.In FinnTwin16, age of alcohol use onset was only measured at the baseline assessment (i.e., at age 16).As a result, age 16 is the maximum age of alcohol use onset shown in the Table, though some individuals may have initiated alcohol use at a later age.T1 = time 1 (age 16 assessment in FinnTwin16; Wave I assessment in Add Health); T2 = time 2 (age 17 assessment in FinnTwin16; Wave II assessment in Add Health); T3 = time 3 (age 18 assessment in FinnTwin16; Wave III assessment in Add Health); T4 = time 4 (age 25 assessment in FinnTwin16; Wave IV assessment in Add Health); T5 = time 5 (age 35 assessment in FinnTwin16; Wave V assessment in Add Health); BMI = body mass index; freq = frequency; relig = religious; sat = satisfaction.

Table 2 .
Parameter estimates from phenotypic mixed-effects growth curve models of alcohol misuse Est = estimate; CI = confidence interval; AIC = Akaike Information Criterion; BIC = Bayesian Information Criterion; LRT = likelihood ratio test.

Table 3 .
Parameter estimates from the biometric mixed−effects growth curve model of alcohol misuse in FinnTwin16