Haemosporidian parasite infections of Malagasy Philepittidae and Nectariniidae are driven by phylogeny rather than ecology

The nectarivorous common sunbird asity (Neodrepanis coruscans) is phylogenetically closely related to the frugivorous velvet asity (Philepitta castanea), yet it shares similar habitat and foraging behaviour as the Malagasy sunbirds (Cinnyris spp.). As ecological factors have been shown to influence blood parasite prevalence, it should be tested whether parasite abundance, prevalence and diversity of N. coruscans are more similar to the sunbirds than to its relative. Therefore, blood samples (n = 156) and smears (n = 60) were tested for different blood parasites (Haemosporida, trypanosomes, filarioid nematodes) using molecular and microscopic methods. High prevalence of haemosporidian parasites was observed in all bird taxa, with rates ranging from 23% in N. coruscans to 84.6% in C. notatus. The Malagasy Cinnyris spp. exhibited a high occurrence of mixed haemosporidian infections (>76%) with various specialized lineages. Within the Philepittidae family, no Haemoproteus infection was detected and just a few cases of mixed infections. Nectariniidae species predominantly had specialized haemosporidian lineages, while Philepittidae had infections mainly caused by generalist lineages. These findings emphasize the diverse range of blood parasites in Nectariniidae, while additionally highlighting the high diversity of trypanosomes and filarioid nematodes in Philepittidae. Additionally, several newly discovered haemosporidian lineages, Trypanosoma isolates and filarioid nematode isolates were identified. Notably, Philepittidae exhibited a lower prevalence of avian haemosporidian parasites compared to Nectariniidae, possibly due to potential resistance mechanisms. Despite N. coruscans sharing similar habitat and behavioural ecology with both Cinnyris spp., it closely resembles its relative, P. castanea, in all aspects of haemosporidian parasitism.


Introduction
The asities (Philepittidae) are a family of old world suboscine birds of Madagascar and form a single radiation of only 4 extant species (Goodman, 2022).Their ancestors likely arrived from Asia to Madagascar approximately 41 million years ago (Warren et al., 2010).These 4 endemic species are divided into 2 different genera (Neodrepanis spp.and Philepitta spp.), which exhibit significant differences in their physical characteristics and behavioural ecology.Neodrepanis are nectar-feeding birds characterized by their slender bill.On the other hand, Philepitta species have shorter beaks (Fig. 1) and are among the few primary frugivorous songbirds in Madagascar.All 4 species exhibit sexual dimorphism in terms of plumage and physical features.The Schlegel's asity (Philepitta schlegeli) is primarily found, even if scarce, in the western forests of Madagascar.In contrast, the velvet asity (Philepitta castanea) inhabits eastern rainforests at altitudes below 1500 m (Hawkins et al., 2015).It tends to be sedentary and is thought to play a role as a seed disperser, primarily foraging on small fruits in the understory shrubs at heights ranging from 2.5 to 5 m.Additionally, they construct elongated globe-shaped nests, typically positioned 2-7 m above the ground (Safford and Hawkins, 2013).Neodrepanis asities were formerly categorized as sunbirds (Nectariniidae) due to the similarities in ecological traits and physical characteristics.One of these species, the yellow-bellied sunbird asity (N.hypoxantha), is found exclusively in high-altitude humid forests between 1200 and 2500 m in eastern Madagascar.Whereas the common sunbird asity (N.coruscans) occurs in mid-altitude rainforests, typically occupying elevations ranging from 500 to 1500 m.It is a small and exceptionally active bird that inhabits the canopy and subcanopy layers.It is often seen accompanying mixed-species flocks (Eguchi et al., 1993) and, occasionally, it competes with the souimanga sunbird (Cinnyris sovimanga) for access to flowers (Safford and Hawkins, 2013).The construction of the nests is similar to that of the velvet asity, featuring a small entrance hole and suspended from vegetation, although typically positioned somewhat higher, around 5-8 m above the ground (Safford and Hawkins, 2013).
The 2 sunbird species endemic to Madagascar occupy similar habitat and behavioural ecology as the Neodrepanis species.In contrast to the asities, sunbirds belong to the oscines and represent a relatively recent radiation, dating back approximately 3.9 million years.It appears that the 2 species arrived on Madagascar through 2 separate colonization events originating from the African continent.They used Indian Ocean islands like the Comoros as stepping stones during their journey from Africa (Warren et al., 2003).The souimanga sunbird (C.sovimanga) is abundantly found in all wooded habitats across Madagascar (Hawkins et al., 2015) and often joins mixed species flocks (Eguchi et al., 1993).It utilizes various vegetation levels, ranging from the canopy of tall forests to shrubbery in clearings, and scattered areas of trees and bushes in open ground (Safford and Hawkins, 2013).The Madagascar green sunbird (Cinnyris notatus) is also distributed throughout Madagascar, with its presence extending up to elevations of 2050 m; however, in forests, it prefers to forage mid-stratum and in the canopy (Hawkins et al., 2015).It can be distinguished from the souimanga sunbird and sunbird asities by its long, strong bill, a larger size and darker plumage of the male (Fig. 1).Both sunbird species show active and restless behaviour.They primarily feed on nectar, but they also capture spiders and insects from flowers.Their strong bills enable them to feed on larger flowers, while flowers with long corollas remain inaccessible to the souimanga sunbird (Safford and Hawkins, 2013).In terms of sexual dimorphism, both species exhibit distinct differences between males and females regarding their plumage and morphology.Females in both species engage in nest construction and incubation, while males are either absent or responsible for defending their territory.The nests built by both species share a design typical for sunbirds, featuring a domed structure with a side entrance.However, the height of these nests varies, with souimanga sunbirds typically placing their nests at 0.5-1.8m above the ground, while Madagascar green sunbird species opt for higher nest locations (Safford and Hawkins, 2013).
Previous studies highlighted that various ecological traits and the geographical distribution of birds exert a significant influence on the abundance, prevalence and diversity of parasites.This pertains specifically to parasites such as protists belonging to the order Haemosporida (Apicomplexa), the genus Trypanosoma (Euglenozoa) and metazoa like filarioid nematodes, all of which share a common habitat at some stage of their life cyclesthe bloodstream.Among these parasites, the haemosporidian genera (Plasmodium, Haemoproteus and Leucocytozoon) develop their gametocytes within the blood cells of their avian hosts.Notably, Plasmodium is the sole genus that also undergoes a phase of asexual reproduction within erythrocytes (Valkiunas, 2005).In contrast, Trypanosomes, while not entirely intracellular parasites like the Haemosporida, reside within the bloodstream or the intestines of their host.Only the amastigote form of the parasite is intracellular (Moretti et al., 2020).Parasitic nematodes, on the other hand, primarily infect host tissues and tissue spaces, with their larval stages occasionally appearing in peripheral blood circulation (Sehgal et al., 2005).All blood parasites play pivotal roles as avian parasites, and exhibit a global distribution and transmission via a variety of blood-sucking insects.They often co-occur in mixed infections (Savage et al., 2009) and in most instances, they do not pose significant harm to their avian hosts.However, it is important to acknowledge that research efforts have been disproportionately allocated among different parasite taxa, with the majority of available data focusing on Plasmodium and Haemoproteus, primarily due to their close relationship with agents responsible for human malaria (Atkinson et al., 2008;Santiago-Alarcon and Marzal, 2020).
The abundance, prevalence and diversity of parasites in different bird species are influenced by various factors, including the extent of host specialization.Specialization plays a crucial role in determining which parasites are capable of infecting a particular host species.Parasites that exhibit a high degree of specialization, focusing on their specific bird hosts, tend to have a narrower host range and consequently a more limited geographic distribution.A case in point is the highly specialized haemosporidian lineages found in Vangas (Vangidae), which are confined to the geographic range of their hosts, primarily Madagascar (Magaña Vázquez et al., 2022).In contrast, generalist avian malaria parasites like Plasmodium relictum are characterized by a global distribution and a remarkable ability to invade diverse host species (Hellgren et al., 2015).Avian Trypanosoma parasites, on the other hand, do not exhibit strict vertebrate-host specificity (Valkiunas et al., 2011), while filarioid nematodes are believed to be highly specialized for their avian hosts (Binkienė et al., 2021).Within the realm of avian Haemosporida parasites, the degree of specialization varies not only between host genera but also among different species within the same genus.In general, Plasmodium and Leucocytozoon species are often considered to be more generalists, capable of infecting a broader range of hosts, whereas Haemoproteus species tend to exhibit a high degree of specialization in most instances.This specialization pattern has been observed in various studies (e.g.Musa et al., 2019;Doussang et al., 2021).
Previous research has demonstrated that the diversity of haemosporidian parasites is closely linked to the richness of avian species (Clark et al., 2014) and that the specific assembly of parasite communities is largely influenced by the composition of their host species (Fecchio et al., 2019).However, the likelihood of birds becoming infected with blood parasites depends on their interaction with the vectors, and this connection is strongly influenced by the ecological characteristics and distribution of these vectors.For instance, in regions with high elevations, there is a notable prevalence of black flies (Simuliidae), which serve as vectors for Leucocytozoon spp.In contrast, areas at lower elevations tend to have a higher abundance of mosquitoes (Culicidae).This altitudinal variation also extends to the distribution of blood parasites.Therefore, Leucocytozoon is more prevalent at higher elevations, followed by Haemoproteus and Plasmodium (Rodríguez-Hernández et al., 2021), whereas trypanosomes and filarioid nematodes are predominantly found at lower elevation areas (Chapa-Vargas et al., 2020).Ornithophilic mosquitoes exhibit greater diversity at the canopy level compared to the forest floor (Chathuranga et al., 2022) which may indicate a higher Plasmodium diversity in the canopy.
Moreover, recent research has identified several ecological and individual traits that play a significant role in affecting the prevalence of haemosporidian parasites.Incidence of infection is directly correlated to the age and sex of birds (Valkiunas, 2005).Furthermore, it has been observed that foraging behaviour exerts an influence on prevalence rates.In particular, the highest prevalence of Plasmodium was found among frugivores, granivores and omnivores, while insectivores exhibited the lowest prevalence (Rodríguez-Hernández et al., 2021).On the other hand, Haemoproteus prevalence was highest among granivores, insectivores and nectarivores, and Leucocytozoon was predominantly present in granivorous and insectivorous birds in Mexico (Rodríguez-Hernández et al., 2021).In open nests, it was noted that the prevalence of Haemoproteus spp.and Leucocytozoon spp. was higher compared to closed nests (Rodríguez-Hernández et al., 2021).This difference in prevalence was attributed to the better protection provided by closed nests for both nestlings and breeding adults (Valkiunas, 2005).
The primary hypothesis investigated in this study posited that the blood parasite abundance, prevalence and diversity in the common sunbird asity (Neodrepanis coruscans) is akin to that of other nectarivorous bird species in Madagascar, specifically the sunbirds (C.notatus and C. sovimanga), due to their similar habitat and behavioural ecology.In contrast, the velvet asity (P.castanea), a relative of the common sunbird asity, is expected to exhibit differing infection rates.Furthermore, it was tested whether (1) the nectarivorous species have lower prevalence for Plasmodium species but a higher diversity in contrast to the frugivorous P. castanea, (2) Nectariniidae and Philepitiidae do have their own specialized Haemoproteus and filarioid nematode species and (3) due to their breeding in closed nests, the prevalence of Haemoproteus and Leucocytozoon of both bird taxa will be lower than in other species at that study site using open cups.

Study site and test material
Birds of the family Nectariniidae and Philepittidae were captured in the Maromizaha tropical rainforest located in the eastern part of Madagascar (18580800 S, 482704800 E), 30 km from the city of Moramanga, at 5 different sampling sites with an altitude between 943 and 1213 m.In the course of a ringing project, birds were caught in mist nets mostly during the breeding season in November and December (2003-2007, 2010, 2012, 2014, 2016, 2018 and 2022).Individual data of birds such as age, sex or weight were measured whenever possible.Birds in juvenile plumage were aged as juveniles until having completed post-juvenile moult.This plumage differs in its colouration and structure.In addition, juveniles often displayed some rest of a gape.Birds lacking juvenile characteristics were categorized as adults, with no further opportunity for a more precise determination of their age.
Before release, a tiny drop of blood was taken by puncturing the brachial vein.The protocol was approved by the Direction de la Préservation de la Biodiversité, Antananarivo, Madagascar.Blood was immediately stored in lysis buffer (Wink, 2006) and, if possible, 1-3 blood smears per bird were prepared before the bird was released back into the wild.A total of 82 blood samples of Nectariniidae (C.sovimanga, n = 56; C. notatus, n = 13) and 74 Philepittidae (P.castanea, n = 61; N. coruscans, n = 13) were collected.Blood smears were prepared for 29 Nectariniidae (C.is sovimanga, n = 26; C. notatus, n = 3) and 31 Philepittidae species (P.castanea, n = 26; N. coruscans, n = 5).The slides were dried on site and fixed with >99% methanol for 10 min in the field.Blood smears from 2022 were Giemsa stained on site using the Hemacolor® staining set (Merck KGaA, Darmstadt, Germany) following the manufacturer's protocol, blood smears of other years were stained in the lab with the same staining set.

Microscopic examination of blood smears
Every slide was examined using an AxioImager M2 (Carl Zeiss AG, Oberkochen, Germany).At first, slides were screened at 400 magnification for 10 min to quickly detect larger parasites 1318 Hannah Barbon et al.

DNA extraction and molecular detection methods of blood parasites
Total DNA extraction of blood samples was performed using the QIAamp DNA Blood Mini Kit (QIAGEN, Hilden, Germany) following the manufacturer's instructions.DNA concentration and purity were quantified using a NanoDrop N50 UV-Vis spectrophotometer (Implen GmbH, Munich, Germany) and stored at -20°C until further use.Molecular parasite detection was performed using different polymerase chain reaction (PCR) setups.
In each test run, a positive control as well as a negative control (nuclease-free water) were included.

Haemosporidian parasites
Detection of haemosporidian infections was done by applying 2 different PCR setups.At first, all samples were screened using a nested PCR targeting a 479 bp region of the cytochrome b gene (cytb) of the 3 parasite genera (Hellgren et al., 2004) following the protocol described by Magaña Vázquez et al. (2022).

Trypanosomes
Presence of Trypanosoma DNA was detected by nested PCR targeting a 770 bp SSU rRNA fragment (Valkiunas et al., 2011).Reaction mixtures were equal to the nested PCR used for haemosporidian detection.The first set of primers was Tryp763 and Tryp1016 and the second pair was Tryp99 and Tryp957.Cycling conditions of both PCRs were performed as described by Valkiunas et al. (2011).Amplification products (5 μL) of the nested PCR were also mixed with GelRed™ stain and then visualized on a 1.5% agarose gel after 20 min at 90 V.

Filarioid nematodes
Screening for microfilariae was performed using a nested PCR assay targeting an approximately 690 bp fragment of the 28S rRNA.Reaction mixtures were equal to the nested PCR used for haemosporidian and Trypanosoma detection.The first set of primers was 28SNemF1 and 28SNemR1 and the second set was 28SNemF2 and 28SNemR2.Cycling conditions of both PCRs were performed as described by Magaña Vázquez et al. (2022).A second nested PCR, targeting a 650 bp fragment of the cox1 gene, was used for samples where DNA amplification of the microfilarial 28S rRNA fragment was successful.Reaction mixtures were equal to the nested PCR targeting the microfilarial 28S rRNA, beside the use of the specific primer set COINemF1/COINemR1 and COINemF2/COINemR2, and cycling conditions were also performed after Magaña Vázquez et al. (2022).

Sequence identification
All amplification products, except those of the nested multiplex PCR, were purified using the PCR Product Purification Kit (Roche, Mannheim, Germany).After sequencing (Microsynth AG, Balgach, Switzerland), the resulting sequence data were checked and edited using Geneious v. 2021.1.1 (https://www.geneious.com).If the chromatogram showed clear double peaks, the sample was considered to contain a mixed infection.The final sequences were then distinguished by identifying their closest matches in GenBank (Benson et al., 2013) using the NCBI nucleotide BLAST search.Avian haemosporidian parasites were additionally identified using the BLAST search of the MalAvi database (Bensch et al., 2009).If haemosporidian sequences differed in at least 1 base pair, it was considered a new lineage and naming was performed according to the system used for the MalAvi database: the first 3 letters of the bird genus, the first 3 letters of the species name, followed by a number (e.g.CINNOT03 from C. notatus).In the text, those lineage names were always accompanied by an abbreviation of its parasite genus (p: Plasmodium, h: Haemoproteus, l: Leucocytozoon).Newly amplified sequences of trypanosomes and filarioid nematodes were named according to their closest match in GenBank and given an isolate-abbreviation for easier identification.All newly detected parasite lineages/ sequences were deposited in GenBank (accession numbers OQ995051-OQ995057, OR148589-OR148300, OR149496-149504, OR159320-OR159331; Supplementary Tables 1-3).

Morphological and molecular-based sexing
Sexing based on morphology was only deemed trustworthy for adult males in breeding plumage and for females presenting a clear brood patch (N = 62).The sex of all other samples was determined using a molecular methodology (N = 74) of Díaz Casana et al. (2019).As a basis of comparison for the PCR approach, 2 samples for which sexing based on morphology was certain were randomly chosen for both sexes in each species (N = 8).PCR reactions were carried out in a total volume of 25 μL, containing 2.5 μL GeneAmp™ 10× PCR Buffer II (Applied Biosystems), 2 μL MgCl 2 (25 mM), 1 μL of each primer (HPF/ HPR; 10 mM), 0.5 μL of each dNTP (10 mol), 0.125 μL AmpliTaq™ DNA polymerase (5 U L −1 ; Applied Biosystems), 2 μL template DNA (1-5 ng μL −1 ) and 15.875 μL nuclease-free water.Cycling conditions were performed after Díaz Casana et al. (2019).Amplification products (10 μL) were mixed with GelRed™ stain and visualized on a 2% agarose gel after 30 min at 90 V.The PCR products in female individuals should show 2 bands, the W band (350-450 bp) and the Z band (500-600 bp), while males show a single band (500-600 bp).Absolute numbers of blood smears (Nbs) and blood samples (N) are given.Results of parasite detections are listed for haemosporidian parasites, trypanosomes and filarioid nematodes, respectively.For each parasite taxon, percentages (%) of infected samples identified with microscopic methods (Pmic) and molecular methods (Pmol), along with the name of the identified lineage/sequence, are given.'-' indicates that percentages were not measurable due to a lack of samples or that no sequence was amplified.If the parasite lineage/sequence was detected more than once, sample sizes are given in brackets.Newly detected lineages/sequences are given in bold letters.

Phylogenetic analyses
Phylogenetic analyses of haemosporidian lineages, trypanosomes and filarioid nematodes detected in this study were performed using MEGA v.10.2 (Kumar et al., 2018).The dataset used for the first phylogenetic approach consisted of cytochrome b sequences of haemosporidian lineages obtained in this study and reference sequences of different morphospecies of Haemosporida downloaded from GenBank, each trimmed to 453 bp to ensure consistency in sequence length.A cytochrome b sequence from Leucocytozoon grallariae (MK103895) was used as an outgroup.The second tree included all 18S rRNA sequences of Trypanosoma detected in the sample set and homologous Trypanosoma sequences from GenBank, each trimmed to 669 bp.The human pathogen Trypanosoma brucei (AF306777) was used as an outgroup.The third and fourth dataset consisted of all sequences of microfilariae detected in this study.Partial sequences of cox1 ( 571) and 28S rRNA (681) were used to perform separate phylogenetic analyses.Ascaridia galli (KY990014 and KT613888) was used as an outgroup in both approaches.Phylogenies were generated by implementing the best-fitting model, which was identified by MEGA v.10.2 for every parasite taxon.All maximum likelihood methods were performed using 1000 replicates.The resulting phylograms were viewed and edited with MEGA v.10.2.

Statistical analyses
Significance of detected differences in prevalence of blood parasite infection between the different bird taxa was measured using the χ 2 -test implemented in R-4.2.1.The influence of age and sex of the birds was also tested with the χ 2 -test.

Degree of specialization
The study utilized the host-diversity index developed by Musa et al. (2019) to assess the level of specialization exhibited by each haemosporidian parasite lineage.The aim was to determine whether specialization occurred at the species, genus or family level, or if the lineages demonstrated a more generalized pattern.This index assigns a value of 1 to indicate maximum host diversity and 0 to signify minimal host diversity.In the context of parasitism, generalist parasite species are capable of infecting a wide range of host taxa, while specialists have a more limited set of potential host taxa.Consequently, parasite species with a high Hd value (>0.6) were categorized as generalists, while those with a value closer to 0 (<0.3) were considered specialists.To arrive at these conclusions, data from the present study and previous research (sourced from MalAvi in June 2023) were compiled.Furthermore, the Hd index was cross-referenced with information from the phylogenetic analysis to provide comprehensive insights into the degree of specialization.
The degree of specialization within each haemosporidian parasite lineage was determined by utilizing the host-diversity index (Hd), as indicated in Supplementary Tables 6 and 7. Notably, an Hd value of 1 was assigned to pGRW04 and pGRW09, as they are acknowledged as generalists.Table 3 included the specialization categorizations that resulted from a combination of Hd values and phylogenetic data.
Infections in Nectariniidae species consist mostly of specialized haemosporidian lineages, whereas infections in Philepittidae are mainly caused by generalist lineages (Fig. 5).pNEWAM07 and hXANZOS01 are considered to be abortive infections as they seem to be specialized on other bird taxa.

Trypanosoma abundance, prevalence and diversity
No trypanosomes were detected in blood smears during microscopic examination.Prevalence of Trypanosoma species detected in blood samples by molecular approach was between 7.7 and 17% in Nectariniidae and Philepittidae (Table 1).Trypanosoma sp.CORVOID01 was the only sequence shared between the families, once detected in C. sovimanga and 3 times in P. castanea.With 8 isolated Trypanosoma sequences, C. sovimanga exhibited the greatest diversity.
In the phylogenetic tree, Trypanosoma sequences detected in this study form 2 separate branches (Fig. 6).One of them contains Trypanosoma avium sequences, including the newly detected  T. avium isolate SOSU, and 2 T. avium species sequences, one of them the isolate SOSU2.Other sequences isolated from Nectariniidae and Philepittidae in this study form the second clade, together with sequences of T. anguiformis and T. bennetti.

Filarioid nematode abundance, prevalence and diversity
Highest prevalence for filarioid nematode infection was detected in C. notatus (31%) using molecular detection methods (Table 1).Other examined bird taxa showed prevalence of 6-13.1%.No sequence was shared between the different bird taxa.
Splendidofilaria bartletti VELAS1 was detected once in a blood smear of P. castanea during microscopic examination (Fig. 7).
Phylogenetic examination was performed separately for 28S rRNA (Fig. 8a) and cox1 (Fig. 8b) sequences of filarioid nematodes because the comparable datasets of molecular markers are very different.In the phylogenetic tree of 28S rRNA sequences, Aproctella alessandroi clusters with Madathamugadia, and Chandlerella species sequences form a distinct clade.The Eufilaria species isolates form another clade, while Onchocercidae and Splendidofilaria species group together, forming a sister clade.This clear separation of groups cannot be seen in the phylogenetic tree using cox1 sequences of the filarioid nematodes.

Mixed infections
Mixed infections of all blood parasite taxa were most abundant in C. notatus (30.7%), fewer in C. sovimanga (18.8%) and P. castanea (14.8%) and lowest in N. coruscans (7.7%).Single infections with haemosporidian parasites were the most common kind of infection in all avian taxa (Table 4).

Molecular sexing
The PCR approach used for sexing gave slightly different results than expected from the original protocol by Díaz Casana et al. (2019).For P. castanea only, female bird samples showed a single band at approximately 400 bp in the agarose gel instead of 2 bands (Fig. 9).This has been approved by 7 samples of birds, Data of other findings are given (other hosts and sites) along with the predicted specialization of each lineage based on the host-diversity index and phylogenetic data.
which have been additionally identified as females by morphological features in the field.
Haemosporidian parasites varied with the age of the different bird host families.In Nectariniidae species, juvenile birds of C. sovimanga were significantly less infected with each haemosporidian genus than adults and showed also lower numbers of multiple infections.Cinnyris notatus showed no significant differences, but combined with the results of C. sovimanga as Nectariniidae, differences were even more significant than with C. sovimanga alone.However, no significant difference was detected in Philepittidae species.For infections with Trypanosoma species or filarioid nematodes the age of the birds did not have a significant effect for either bird taxa.

Discussion
The objective of this study was to investigate whether the blood parasite composition in the Malagasy common sunbird asity (N.coruscans) is influenced by ecological traits or by its phylogenetic background.Our initial hypothesis suggested that Nectariniidae  Parasitology 1325 species (C.sovimanga and C. notatus) would share similar parasite compositions and prevalence rates with N. coruscans, despite belonging to different avian families, due to their similarities in habitat and behavioural ecology.However, our findings did not align with this expectation.We observed significant variations in parasite prevalence and diversity between N. coruscans and the Cinnyris species.Instead, the parasite composition in N. coruscans appeared to be more akin to that of its close relative, P. castanea, particularly concerning haemosporidian parasites.
In both Nectariniidae species, a substantial number of haemosporidian lineages were detected, and there was also a high diversity of these lineages.The majority of the samples showed evidence of mixed infections, with triple infections involving all 3 genera being the most prevalent type.Specialized haemosporidian lineages appear to exist in both Malagasy sunbird species.Neodrepanis coruscans showed the lowest prevalence of haemosporidian infections and was only found to be infected with generalist Leucocytozoon lineages.Philepitta castanea also harboured these lineages but additionally exhibited infection with a potentially specialized Leucocytozoon lineage (lPHICAS01) and a few other generalist Plasmodium lineages.Notably, the occurrence of mixed infections was significantly lower in both Philepittidae species compared to the Nectariniidae species.The absence of triple infections involving different haemosporidian genera was attributed to the complete absence of Haemoproteus lineages in Philepittidae species.Given the absence of reports of generalist Haemoproteus species in Madagascar so far, the lack of Haemoproteus spp. in Philepittidae species may suggest a resistance against specialized Haemoproteus species within the study area.The absence of specialized haemosporidian lineages in the Philepittidae species is quite surprising.Among these lineages, lPHICAS01 appears to be the only one that might be specialized or even prefers to infect P. castanea, as it has been just sporadically detected in 2 other bird species (Musa et al., 2022).Phylogenetic analysis revealed a very close relationship (with only 4 out of 479 base pairs differing) between lPHICAS01 and the generalist lineage lHYPMA02, which was also detected in both Philepittidae species.It is possible that lPHICAS01 is in the process of developing into a specialized lineage.
The Philepittidae family of birds has ancient origins, with its roots tracing back to Asia, where it embarked on a radiation process in Madagascar over 40 million years ago (Warren et al., 2010).It is plausible that the ancestors of the Philepittidae were initially infected solely by generalist haemosporidian lineages that maintained their host range throughout this extended period.Interestingly, there is no evidence of speciation on the same host  (with the exception of lPHICAS01) or host switching events that led to the development of specialized haemosporidian species within this family.In contrast, the Nectariniidae family arrived in Madagascar roughly 3 million years ago from Africa (Warren et al., 2003).It is possible that they already harboured specialized lineages, such as pCOSUN2, prior to their colonization of Madagascar.Subsequent to their arrival on the island, haemosporidian parasites within the Cinnyris species underwent further diversification, resulting in a broad array of specialized lineages.Phylogenetic analysis suggests that the specialized lineages (lCINSOV02, lCINSOV04-lCINSOV06) may have evolved from the generalist lineage lFOMAD01.However, it remains unclear why this radiation occurred in Nectariniidae but not in Philepittidae species, which are also suitable hosts for lFOMAD01.One possible explanation is that Philepittidae species exhibit a higher tolerance to these parasites, thereby reducing the selection pressure that would drive parasite evolution towards specialization.In lineages presumed to be already specialized, as observed in Nectariniidae, there exists a certain degree of selection pressure due to coevolutionary dynamics, which favours further diversification.It would be valuable for future studies to focus on ancient bird taxa and investigate whether this observed phenomenon holds true more broadly across different avian families.Data from 2 bird taxa at the same study site, which, unlike the species examined in this study, construct open nests, were utilized to explore the impact of nest construction on haemosporidian prevalence.In the Madagascar paradise flycatcher (Terpsiphone mutata), the prevalence of Haemoproteus and Leucocytozoon infection was found to be 0% (Musa et al., 2019), while of Vangidae showed prevalence rates in the range of 45-52% (Magaña Vázquez et al., 2022).Similarly, notable differences in prevalence were observed within the studied bird families that utilize closed nests (Nectariniidae exhibited a prevalence of 63-70%, whereas Philepittidae showed 0% prevalence for Haemoproteus and 31% for Leucocytozoon).Consequently, it can be concluded that the nesting behaviour of the studied bird species did not have a discernible effect on the prevalence of Haemoproteus and Leucocytozoon when compared to bird species employing open cups for nesting at the same study site.
The age of the birds had a notable impact on avian haemosporidian prevalence within the Nectariniidae family, but this influence was not observed in the Philepittidae.In adult birds, both prevalence and the occurrence of mixed infections were higher compared to juveniles, and this can be attributed to the multiplication of infections over time.Haemosporidian parasites are known to persist in bird hosts for extended periods, potentially spanning years or even a lifetime (Valkiunas, 2005).Consequently, the likelihood of being infected with haemosporidian parasites increases with the age of the host (Slowinski et al., 2021).Conversely, no such age-related effect was observed in Philepittidae species.This discrepancy might be attributed to the relatively low diversity of parasites within this avian taxon.Given that the pool of suitable parasites for Philepittidae appears to be limited, the maximum number of potential infections within the population may be quickly reached.
The prevalence of Trypanosoma and filarial nematodes exhibited similarity across different avian taxa.Neither the distinct ecological niches nor the phylogenetic backgrounds of these birds appear to result in variations in the likelihood of infection with these blood parasites.Significant differences in prevalence were not found based on the sex and age of the various bird species.While age-related differences have been documented in Phasianidae (Holmstad et al., 2003) and Accipitridae (Svobodová et al., 2015), no such variations were identified in Passeriformes, such as the pied flycatcher (Muscicapidae) (Merino and Potti, 1995).The study by Svobodová et al. (2015),  Parasitology which involved recaptures of Accipitridae, suggested that Trypanosoma infection persists throughout the lifetime of these birds.This finding elucidates the observed differences in prevalence between juveniles and adults.Conversely, since no such age-related discrepancy has been reported thus far in Passeriformes, it is reasonable to assume that Trypanosoma infections in these avian taxa are not of a long-lasting nature.Moreover, as there were no discernible differences in prevalence between the sexes and age groups of birds concerning microfilariae infections, it can be inferred that these infections also do not typically result in permanent, chronic infections.Additional research is needed to delve deeper into this subject.Mixed infections involving both blood parasite taxa, Trypanosoma and filarioid nematodes, were frequently encountered.However, instances of double infections, where both Trypanosoma and filarioid nematodes were present together, were not observed.This outcome implies a potential positive correlation wherein haemosporidian infection might enhance the likelihood of acquiring infections with other blood parasite taxa.Infections with haemosporidian parasites, but not with Trypanosoma and filarioid nematodes, may lead to a decrease in host fitness and an increased vulnerability to other infections.
Host specialization is likely to be the case for filarioid nematodes, given the clear phylogenetic separation of their lineages and the absence of a single sequence detected in different bird species.On the other hand, the proposed generalism of avian trypanosomes (Zídková et al., 2012) is supported by the presence of Trypanosoma sp.CORVOID01 in both Nectariniidae and Philepittidae.Nevertheless, the identification of a substantial number of newly discovered Trypanosoma sequences exclusively in a single bird species may suggest the existence of host-specific haplotypes.However, making more precise conclusions is challenging due to limited data availability for trypanosomes and the unclear systematics in this context.
In summary, Nectariniidae and Philepittidae exhibit disparities in terms of haemosporidian parasite prevalence, diversity and specialization, while no such distinctions are apparent in the case of Trypanosoma sp. or filarioid nematode infections.Despite N. coruscans sharing similarities in habitat and behavioural ecology with both Cinnyris spp., it closely resembles its relative, P.ta castanea, in all aspects of parasitism.The initial hypothesis suggesting that nectarivorous species would display lower prevalence but higher diversity in contrast to the frugivorous P. castanea was not supported by our findings.Specialized Haemoproteus lineages have only been proposed for Nectariniidae species, as Philepittidae lack these parasites.However, specialized filarioid nematode species are detected in both avian families.Interestingly, the construction of closed nests or feeding behaviour does not appear to significantly influence the likelihood of infection with Haemoproteus and Leucocytozoon spp. in the examined bird species.Haemosporidian parasite infections in Malagasy Philepittidae and Nectariniidae appear to be primarily driven by phylogenetic factors rather than ecological ones, whereas Trypanosoma and filarioid nematode infections do not exhibit a clear association with either factor.Supplementary material.The supplementary material for this article can be found at https://doi.org/10.1017/S0031182023001075. Data availability statement.The authors confirm that the data supporting the findings of this study are available within the article and its supplementary materials.

Figure 6 .
Figure 6.Phylogenetic relationship of Trypanosoma SSU rRNA sequences (Acc.No.) detected in blood samples of Nectariniidae and Philepittidae species on Madagascar, along with highly homologous sequences of Trypanosoma species deposited in GenBank (Acc.No.), constructed using maximum likelihood (K2 + G).Dots on nodes indicate bootstrap values >70%.Bold text indicates new sequences.

Figure 8 .
Figure 8. Phylogenetic relationship of filarioid nematode 28S rRNA (a) and cox1 (b) sequences detected in blood samples of Nectariniidae and Philepittidae species on Madagascar, along with highly homologous sequences of avian filarioid nematodes deposited in GenBank (Acc.No.), constructed using maximum likelihood (a: T92; b: TN93).Bootstrap values are given.Bold text indicates new sequences.

Table 1 .
Dataset of Nectariniidae and Philepittidae species examined in this study