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Morphological and molecular characterization of Paractinolaimus sahandi n. sp. (Nematoda: Actinolaimidae) from the Sahand Mountains in Iran

Published online by Cambridge University Press:  21 September 2010

M. Pedram ,
G. Niknam ,
M.T. Vinciguerra ,
W. Ye  and
R.T. Robbins
M. Pedram
Affiliation:
Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran College of Agriculture, University of Tarbiat Modarres, Tehran, Iran
G. Niknam*
Affiliation:
Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
M.T. Vinciguerra
Affiliation:
Dipartimento di Biologia Animale, Università di Catania, via Androne 81, I-95124Catania, Italy
W. Ye
Affiliation:
Nematode Assay Section, North Carolina Department of Agriculture, Raleigh, North Carolina, USA
R.T. Robbins
Affiliation:
Department of Plant Pathology, University of Arkansas, Fayetteville, Arkansas, USA
*
*Fax: 0098 411 3356006 E-mail: g_niknam@tabrizu.ac.ir
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Abstract

Paractinolaimus sahandi n. sp., found in wet soil samples collected from the rhizosphere of grasses of Sahand Mountains, Iran, is described. This new species is characterized by its long body (3.5–4.7 mm), high a value (74.5–88.5), anterior location of posterior subventral nuclei, occupying 62.5–68.0% of glandularium distance, the presence of 1–4 pre- and 1–3 post-vulval papillae and numerous tiny, not innervated papillae in front and behind the vulva in the outer layer of cuticle; common functional males in the population, with 62.5–81.3 μm long spicules and 15–17 ventromedian supplements. The new species, which is the only one in the genus showing the advulval cuticular tiny papillae and is unusually slender, is compared to four species of Paractinolaimus, namely P. macrolaimus, P. longidrilus, P. spanithelus and P. rafiqi. The ribosomal 18S rDNA (1246 bp sequenced) and 28S rDNA D2/D3 region (844 bp sequenced) of P. sahandi n. sp. were sequenced for molecular characterization. Sequences of the 18S and 28S D2/D3 of P. sahandi n. sp. have distinct differences from those of the only sequenced P. macrolaimus, with 6 bp differences in 18S and 38 bp differences and five gaps in 28S. This is the first report of the occurrence of members of Actinolaimidae in Iran.

Type
Research Papers
Information
Journal of Helminthology , Volume 85 , Issue 3 , September 2011 , pp. 276 - 282
Copyright
Copyright © Cambridge University Press 2010

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References

Alther, E. (1968) Nematodes de la nappe phreatique de resau fluvial de la Saale (Thuringe) et psammique du Lac Stechlin (Brandenbourg du nord). Limnologica, Berlin 6, 247–320.Google Scholar
Andrássy, I. (1964) Süsswasser Nematoden aus den Grossen Gebirgsgegenden Ostafrikas. Acta Zoologica Academiae Scientiarum Hungaricae 10, 1–59.Google Scholar
Andrássy, I. (1998) Once more: the oesophageal gland nuclei in the dorylaimoid nematodes. Opuscula Zoologica Budapestinensis 31, 165–170.Google Scholar
Andrássy, I. (2006) Free-living nematodes of Hungary (nematoda errentia) ш. Budapest, Hungarian History Museum.Google Scholar
De Grisse, A.T. (1969) Redescription ou modifications de quelques technique utilisées dans l'étude des nematodes phytoparasitaires. Mededelingen Rijksfakulteit Landbouwhogeschool Gent 34, 351–369.Google Scholar
De Ley, I.T., De Ley, P., Vierstraete, A., Karssen, G., Moens, M. & Vanfleteren, J. (2002) Phylogenetic analysis of Meloidogyne small subunit rDNA. Journal of Nematology 34, 319–327.Google ScholarPubMed
De Man, J.G. (1880) Die einheimischen, frei in der reinen Erde und im süssen wasser lebenden Nematoden. Tijdschrift der Nederlandsche Dierkundige Veereiniging 2, 78–196.Google Scholar
Dorris, M., Viney, M.E. & Blaxter, M.L. (2002) Molecular phylogenetic analysis of the genus Strongyloides and related nematodes. International Journal of Parasitology 32, 1507–1517.CrossRefGoogle ScholarPubMed
Eveleigh, E.S. (1982) Two new species of Paractinolaimus Meyl, 1957 (Nematoda: Paractinolaimidae) from the Mackenzie and Porcupine river systems, Northwest Territories, Canada. Canadian Journal of Zoology 60, 158–164.CrossRefGoogle Scholar
Floyd, R., Abeb, E., Papert, A. & Blaxter, M. (2002) Molecular barcodes for soil nematode identification. Molecular Ecology 11, 839–850.CrossRefGoogle ScholarPubMed
Khan, Z. & Jairajpuri, M.J. (1998) Description of Paractinolaimus rafiqi sp. n. (Actinolaimoidea: Dorylaimida) from Nepal. International Jounal of Nematology 8, 68–70.Google Scholar
Loof, P.A.A. (1961) The nematode collection of Dr. J.G. de Man, 1. Beaufortia 8, 169–254.Google Scholar
Mullin, P.G., Harris, T.S. & Powers, T.O. (2005) Phylogenetic relationships of Nygolaimina and Dorylaimina (Nematoda: Dorylaimida) inferred from small subunit ribosomal DNA sequences. Nematology 7, 59–79.CrossRefGoogle Scholar
Nunn, G.B. (1992) Nematode molecular evolution. Unpublished PhD dissertation, University of Nottingham, UK.Google Scholar
Thorne, G. (1939) A monograph of the nematodes of the superfamily Dorylaimoidea. Capita Zoologica 8, 1–261.Google Scholar
Vrain, T.C., Wakarchuk, D.A., Levesque, A.C. & Hamilton, R.I. (1992) Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group. Journal of Nematology 29, 250–254.Google Scholar