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Genetic analysis of brown planthopper biotypes using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR)

Published online by Cambridge University Press:  08 April 2017

K. A. Shufran  and
M. E. Whalon
K. A. Shufran
Affiliation:
Department of Entomology and Pesticide Research Center, Michigan State University, East Lansing, Michigan 48824, USA
M. E. Whalon
Affiliation:
Department of Entomology and Pesticide Research Center, Michigan State University, East Lansing, Michigan 48824, USA
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Abstract

Random amplified polymorphic DNA (RAPD) amplified by the polymerase chain reaction (PCR) was used to estimate the relatedness of three biotypes of brown planthopper, Nilaparvata lugens (Stål), from the Philippines. Each individual brown planthopper had a unique set of RAPD bands, but no bands were diagnostic for any one biotype. Brown planthopper types 1, 2 and 3 were genetically homogeneous. This supports results of other studies which conclude that brown planthopper biotypes are not sub-specific categories, but merely represent individual variation for virulence to rice cultivars.

Résumé

Le random amplified polymorphic DNA amplifié au PCR (RAPD-PCR) a été utilisé pour determiner les liens de parenté de trois biotypes de la sauterelle brune, Nilaparvata lugens (Stål), provenant des Philippines. Chaque sauterelle brune a un lot unique de band RAPD mais aucune bande n'a été identifiée pour un quelconque biotype. Les biotypes 1, 2 et 3 de la sauterelle brune était génétiquement homogenes. Ce resultat est en accord avec ceux d'autres études qui ont conclu que les biotypes de la sauterelle brune ne sont pas des catégories subspécifiques mais ils représentent plutôt des variations individuelles de virulence à l'encontre des cultivars de riz.

Keywords

biotypebrown planthopperNilaparvata lugensRAPD-PCRbiotypesauterelle bruneNilaparvata lugensRAPD-PCR
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 16 , Issue 1 , March 1995 , pp. 27 - 33
Copyright
Copyright © ICIPE 1995

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